Article
Agriculture, Multidisciplinary
Wei Song, Kai Zheng, Xin Xu, Cong Gao, Liang Guo, Jia Liu, Xiulai Chen, Liming Liu, Guipeng Hu, Jing Wu
Summary: Protein engineering was used to enhance the phosphorylation efficiency of Pseudomonas aeruginosa acid phosphatase for AsA-2P production, resulting in a mutant with significantly increased enzyme activity and conversion rate, showing great potential for large-scale production of AsA-2P.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Thibaud Laffargue, Claire Moulis, Magali Remaud-Simeon
Summary: Polysaccharides are potential candidates for future polymer materials due to their biological origin and degradability. The functionalization through phosphorylation can enhance their properties, leading to a wider range of applications. Enzymatic phosphorylation offers a promising alternative to chemical phosphorylation for polysaccharides, considering the unresolved metabolic pathways and the diversity of kinase sequences.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Chemistry, Physical
Yunjie Li, Ting Shi, Pingping Han, Chun You
Summary: An in vitro synthetic enzymatic biosystem was designed and constructed for the thermodynamics-driven production of D-allulose from low-cost starch, achieving high product yields. This strategy could significantly reduce the production cost of D-allulose and provide a promising alternative for the cost-efficient production of other rare sugars on an industrial scale.
Article
Microbiology
Krishna Kumar Singh, P. J. Athira, Neerupma Bhardwaj, Devendra Pratap Singh, Uchenna Watson, Deepak Kumar Saini
Summary: The study demonstrates that the essential response regulator protein MtrA in Mycobacterium tuberculosis undergoes non-enzymatic acetylation through acetyl phosphate, independent of phosphorylation. Acetylation of MtrA enhances its phosphorylation and interaction with its sensor kinase MtrB, while also affecting its DNA-binding ability on gene targets. Acetylation is proposed as a dominant post-translational modification overriding phosphorylation to regulate the activities of MtrA in M. tuberculosis.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Immunology
Frederic Bornancin, Carien Dekker
Summary: This review focuses on the regulation of the NLRP3 inflammasome by phosphorylation and dephosphorylation, and discusses the kinases and phosphatases that have been reported to modulate NLRP3 activity. The aim is to integrate the current understanding and highlight potential gaps for further studies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Enrico Benassi, Haiyan Fan, Qinglei Sun, Kanat Dukenbayev, Qian Wang, Ainur Shaimoldina, Aigerim Tassanbiyeva, Lazzat Nurtay, Ayan Nurkesh, Aidana Kutzhanova, Chenglin Mu, Adilet Dautov, Madina Razbekova, Anar Kabylda, Qing Yang, Ziye Li, Amr Amin, Xugang Li, Yingqiu Xie
Summary: Processed herbs exhibit phosphatase-like activity, which can effectively inhibit cancer cell growth via phosphatase signaling and potentially interact with kinase signaling or directly bind/unwind DNA. The study introduces a new term "herbzyme" to describe the enzymatic/co-enzymatic activity attributed to the nanosized assemblies in herbs and herbal food beyond their known pharmacological properties.
NANOSCALE ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Thijs W. van Veldhuisen, Wiggert J. Altenburg, Madelief A. M. Verwiel, Lenne J. M. Lemmens, Alexander F. Mason, Maarten Merkx, Luc Brunsveld, Jan C. M. van Hest
Summary: Membraneless organelles play a crucial role in spatial organization and regulation of intracellular processes. This study presents a coacervate system that utilizes the 14-3-3 scaffold protein to investigate enzymatically regulated recruitment of 14-3-3-binding proteins. Synthetic coacervates efficiently load 14-3-3 and phosphorylated binding partners, allowing for increased local concentration and controlled release of proteins.
ADVANCED MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Shutang Tan, Christian Luschnig, Jiri Friml
Summary: The plant hormone auxin is central to plant growth and development, with protein phosphorylation emerging as a crucial post-translational modification type regulating auxin output. This review comprehensively discusses the genetics, biochemistry, and cell biology of kinases and phosphatases involved in auxin action, providing insights into the mechanisms of developmental auxin responses and future directions for integrating protein phosphorylation control into the molecular auxin network.
Article
Chemistry, Applied
Menghan Wu, Yanning Li, Jinge Li, Shuang Xu, Zhengbiao Gu, Li Cheng, Yan Hong
Summary: In this study, a method for synthesizing starch phosphate using the transferase properties of alkaline phosphatase was successfully explored. The crystallinity and enthalpy of the phosphorylated samples were reduced by up to 26.8% and 23.3%, respectively, and the grafting of phosphate radicals was further confirmed by X-ray photoelectron spectroscopy and time of flight secondary ion mass spectrometry.
CARBOHYDRATE POLYMERS
(2022)
Article
Cell Biology
Gaifen Li, Jia Liu, Mengyuan Guo, Yakun Gu, Yuying Guan, Qianqian Shao, Wei Ma, Xunming Ji
Summary: Chronic hypoxia leads to irreversible cognitive impairment, primarily due to hippocampal neurodegeneration. The underlying mechanism of this process is poorly understood. This study found that chronic hypoxia can induce alpha-synuclein hyperphosphorylation, protein aggregation, hippocampal neurodegeneration, and cognitive deficits. It was also discovered that chronic hypoxia enhances ceramide catabolism, leading to the inhibition of protein phosphatase 2A and sustained induction of alpha-synuclein hyperphosphorylation. Intermittent hypoxic preconditioning can protect against chronic hypoxia-induced hippocampal neurodegeneration and cognitive impairment by preventing alpha-synuclein hyperphosphorylation. This research reveals a novel mechanism underlying alpha-synuclein pathology during chronic hypoxia and has implications for developing therapeutic interventions for chronic hypoxia-related brain disorders.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Elena I. Ilina, Camille Cialini, Dietlind L. Gerloff, Maitane Duarte Garcia-Escudero, Celine Jeanty, Marie-Laetitia Thezenas, Antoine Lesur, Vincent Puard, Francois Bernardin, Alina Moter, Anne Schuster, Monika Dieterle, Anna Golebiewska, Jean-Jacques Gerardy, Gunnar Dittmar, Simone P. Niclou, Tanja Mueller, Michel Mittelbronn
Summary: This study demonstrated that the expression of glycosyltransferase GLT8D1 is significantly higher in GBM patients compared to normal brain tissue, and it may be associated with decreased patient survival. In vitro experiments showed that increased GLT8D1 expression enhances GBM cell migration. Point mutation experiments revealed that the active site of GLT8D1 is essential for its glycosyltransferase activity and cell migration. Furthermore, the study identified potential interaction partners of GLT8D1 related to cytoskeleton and intracellular transport.
Article
Chemistry, Physical
Meihui Yi, Jiaqi Guo, Hongjian He, Weiyi Tan, Nya Harmon, Kesete Ghebreyessus, Bing Xu
Summary: This study demonstrates novel enzyme triggers, phosphonaphthyl (pNP) and phosphobiphenyl (pBP) motifs, which act faster than phosphotyrosine for enzymatic self-assembly and hydrogelation.
Article
Chemistry, Analytical
Ke Xiao, Rong Zhu, Cuicui Du, Hejie Zheng, Xiaohua Zhang, Jinhua Chen
Summary: A self-powered photoelectrochemical (PEC) sensor using zinc-air batteries as a power source was developed for activity assay of protein tyrosine phosphatase 1B (PTP1B). The sensor showed superior performance with a broad linear response range and low detection limit, indicating great potential in the diagnosis of protein phosphatase-related diseases and the development of targeted drugs.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Ying Wang, Xin Li, Dequan Zhang, Zheng Li, Baocai Xu, Jie Zhu
Summary: This study investigated the effect of phosphorylation on titin degradation and found that treatment with alkaline phosphatase could make titin more susceptible to degradation by mu-calpain.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Chemistry, Applied
Jielong Zhang, Dasong Liu, Xiumei Tao, Jun Tang, Xiaoyu Peng, Thom Huppertz, Xiaoming Liu, Peng Zhou
Summary: The effect of enzymatic dephosphorylation on the structure of casein micelles in caprine micellar casein concentrate was studied. The results showed that with increasing dephosphorylation, the molar mass decreased, the radii decreased, the hydration increased, and the internal protein inhomogeneity disappeared.
FOOD HYDROCOLLOIDS
(2024)
Article
Biotechnology & Applied Microbiology
Marina Simona Robescu, Mattia Niero, Melanie Hall, Laura Cendron, Elisabetta Bergantino
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Michael Lukesch, Gabor Tasnadi, Klaus Ditrich, Melanie Hall, Kurt Faber
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2020)
Article
Chemistry, Multidisciplinary
Gabor Tasnadi, Marcin Stasko, Klaus Ditrich, Melanie Hall, Kurt Faber
Article
Electrochemistry
Wolfgang Jud, C. Oliver Kappe, David Cantillo
Article
Chemistry, Organic
Bence Barna, Tamas Gati, Andras Kotschy, Gabor Tasnadi
Summary: This study developed a one-pot two-step enzymatic approach for the functionalization of THIQs. The method is efficient and simple, providing valuable building blocks for organic synthesis.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Physical
Vladimir Kren, Wolfgang Kroutil, Melanie Hall
Summary: Kurt Faber, a synthetic organic chemist, has made significant contributions to the field of biocatalysis. His work has recognized the importance of natural catalysts in organic synthesis and has provided a solid foundation for this discipline. He has also been involved in teaching and mentoring young scientists, and his book "Biotransformations in Organic Chemistry" has become a renowned resource for chemists and biotechnologists. In recognition of his innovative contributions, he received the Biotrans senior award at the Biotrans 2021 symposium.
Article
Chemistry, Physical
Marina S. Robescu, Laura Cendron, Arianna Bacchin, Karla Wagner, Tamara Reiter, Ignacy Janicki, Kemal Merusic, Maximilian Illek, Matteo Aleotti, Elisabetta Bergantino, Melanie Hall
Summary: This study reports the asymmetric isomerization of a prochiral model substrate by two stereocomplementary OYES enzymes, originating from algae and fungi. The enzymes operate through asymmetric proton transfer and achieve high enantiomeric excess (>99% ee). Mechanistic studies reveal the crucial role of partially nonconserved tyrosine residues for the activity and stereoselectivity of the isomerases. The findings offer insights into the potential of flavoproteins in nonredox catalysis and provide hints for discovering olefin isomerases in stereodivergent classes of OYES enzymes.
Review
Biotechnology & Applied Microbiology
Andras Telek, Zsofia Molnar, Beata G. Vertessy, Gabor Tasnadi
Summary: This review summarizes the current knowledge of opines and opine-type chemicals, highlighting their diverse biochemical roles and potential as synthetic building blocks of bioactive compounds. The synthesis of opines involves the reductive amination of ketoacids with amino acids, which has high synthetic potential in producing enantiopure secondary amines. While only one enzyme has been used as a biocatalyst for this transformation, analysis suggests that more enzymes can be exploited in synthetic organic chemistry.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Organic
Nandor Gyorfi, Gabor Tasnadi, Mario Gyuris, Andras Kotschy
Summary: The photoredox three-component reaction utilizing α-acyloxy-N-heterocycles as radical precursors, styrene derivatives as radical trapping agents, and alcohols as nucleophilic quenchers enables the synthesis of a diverse set of branched ethers with high structural complexity. This reaction shows potential for the synthesis of complex drug derivatives and is easily scalable for industrial production. Mechanistic insights and limitations of the reaction are also discussed.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sebastian Roth, Somayyeh Gandomkar, Federico Rossi, Melanie Hall
Summary: This study explores the biocatalytic potential of bacterial enzymes OplA and OplB for the hydrolysis of medium-sized lactams. By optimizing reaction conditions, improving protein expression and stability, and using a cheap recycling system for ATP, high conversion rates were achieved.
Review
Biochemistry & Molecular Biology
Melanie Hall
Summary: Enzymes have gained momentum at the turn of the 21st century, especially in synthetic organic chemistry, where they are increasingly being applied in a variety of processes. Synthetic chemists are recognizing the value of enzymes for their selectivity, paving the way for their use in asymmetric synthesis. The field has matured over the past two decades, proposing a panel of catalytically diverse enzymes for stereoselective reactions, enantioselective replacements, and atroposelective reactions.
RSC CHEMICAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Flora Nagy, Imre Gyujto, Gabor Tasnadi, Bence Barna, Diana Balogh-Weiser, Kurt Faber, Laszlo Poppe, Melanie Hall
JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Erika Tassano, Kemal Merusic, Isa Buljubasic, Olivia Laggner, Tamara Reiter, Andreas Vogel, Melanie Hall
CHEMICAL COMMUNICATIONS
(2020)
