Article
Biochemistry & Molecular Biology
Christian Fernandez, Andres Burgos, Diego Morales, Roberto Rosales-Rojas, Javiera Canelo, Ariela Vergara-Jaque, Gabriel Viliod Vieira, Rodrigo Alberto Alves da Silva, Katiuchia Uzzun Sales, Michael J. Conboy, Eun Ji Bae, Kang-Sik Park, Vicente A. Torres, Mauricio Garrido, Oscar Cerda, Irina M. Conboy, Monica Caceres
Summary: TMPRSS11a is an age-specific protein that plays a role in skin wound healing, with levels increasing with age. Overexpression of TMPRSS11a inhibits cell migration and spreading, leading to cellular senescence.
Review
Chemistry, Multidisciplinary
Afsana Sheikh, Shadab Md, Prashant Kesharwani
Summary: A bird's eye view is needed in cancer research to address the shortcomings of chemotherapy, with targeted therapy showing promise in providing more efficient treatment for tumor cells.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Oncology
Mark Sutherland, Andrew Gordon, Fatemah O. F. O. Al-Shammari, Adam Throup, Amy Cilia La Corte, Helen Philippou, Steven D. Shnyder, Laurence H. Patterson, Helen M. Sheldrake
Summary: This study presents a new ligand-mimetic beta 3 integrin antagonist that exhibits high activity against alpha v beta 3 and moderate affinity for alpha IIb beta 3, suggesting a new approach to integrin targeting in cancer.
Article
Biochemistry & Molecular Biology
Cynthia L. Schreiber, Canjia Zhai, Bradley D. Smith
Summary: The new class of deep-red fluorescent probes SF8 exhibit high fluorescence brightness and stability, making them suitable for fluorescence cell microscopy research. By synthesizing and evaluating different SF8 probes for probe uptake by cancer cells, it was found that the probe with the RGD motif permeated the cells through receptor-mediated endocytosis, while the other two homologous SF8 probes showed negligible cell uptake, indicating differences in targeting capability. Additionally, the synthetic method allows for easy alteration of the peptide sequence, enabling the development of new peptidyl SF8 probes targeting other cancer biomarkers.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xiaocui Zheng, Yiran Li, Tongxiao Cui, Jing Yang, Xiangfeng Meng, Haiqi Wang, Liusheng Chen, Jian He, Nan Chen, Liying Meng, Lin Ding, Ran Xie
Summary: Executing precise glycan editing is crucial for understanding glycan-related biological processes and improving disease therapy. This study introduces a versatile and traceless glycan modification method that allows customization of targeted protein glycoforms through chemical and photoregulation. The method minimizes interference and enables precise temporal control of editing events.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Medicinal
Yajun Chen, Lixuan Jia, Guilan Zhu, Wei Wang, Ming Geng, Hongxia Lu, Yan Zhang, Minghui Zhou, Fangyan Zhang, Xiaozhong Cheng
Summary: Cyclic arginine-glycine-aspartic (RGD) peptides with specific binding ability to integrin alpha nu beta 3 have been developed for drug delivery, tracing, and imaging in tumor diagnosis and treatment. In this study, a series of polycyclic RGD peptides with dual, tri, and tetra rings were designed and synthesized using sortase A-mediated ligation. In vitro cell adhesion inhibition tests revealed that the tricyclic RGD peptide exhibited outstanding potency and selectivity for alpha nu beta 3 integrin.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Immunology
Christopher A. Beaudoin, Samir W. Hamaia, Christopher L. -H. Huang, Tom L. Blundell, Antony P. Jackson
Summary: The spike protein of SARS-CoV-2 may interact with integrins independently of the RGD sequence, as suggested by studies showing changes in integrin expression during infection and high sequence similarity of integrin-binding motifs with regions of the spike receptor-binding domain.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Tamas Gerecsei, Peter Chrenko, Nicolett Kanyo, Beatrix Peter, Attila Bonyar, Inna Szekacs, Balint Szabo, Robert Horvath
Summary: This study utilized a computer-controlled micropipette (CCMP) to measure the dissociation constant (K-d) of integrin-RGD binding, combined with techniques such as surface coating and local flow inducing hydrodynamic lifting force to study cell adhesion behavior. The results demonstrated that CCMP could effectively characterize cell adhesion at both single-cell and whole population levels, offering a convenient and automated approach for quantifying cell adhesivity before further processing.
Article
Materials Science, Biomaterials
Jie Dai, Zixing Xu, Jinhua Xu, Huoyue Lin, Xuan Yang, Jun Wang, Gang Ruan
Summary: The challenge of biological delivery in biotechnology is due to the need to overcome multiple barriers. SDot nanoparticles were designed with a hydrophobic bio-interface to enhance interactions with lipid membranes. In experiments, PTX@SDots-RGD displayed excellent intracellular targeting ability to microtubules in U87MG cells, suggesting the potential of SDots-RGD for bio-delivery.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Materials Science, Multidisciplinary
Ping Zhou, Fang Feng, Yameng Song, Jing Li, Qin Li, Zerong Xu, Jiamin Shi, Liying Qin, Fei He, Hongjiao Li, Yu Han, Rongzhi Zhang, Huanxiang Liu, Feng Lan
Summary: The design of novel peptide sequences and investigation of cell adhesion mechanisms are crucial for hiPSCs culture on peptide displaying surfaces. This study reveals the importance of near-Asp sequences in RGD-containing peptides and presents a novel peptide, Ac-KGGVFTMPRGDTYRAY, with excellent ability to sustain hiPSCs cultures. Structural modeling and molecular docking confirm the strong affinity between the peptide and ocV133 integrin protein, contributing to its superior performance. These findings shed light on the mechanisms underlying RGD-containing peptides' support of adhesion and offer a better peptide option for hiPSCs culture.
MATERIALS & DESIGN
(2022)
Article
Cardiac & Cardiovascular Systems
Yihui Wang, Jizheng Wang, Ling Shi, Xiuyu Chen, Desheng Li, Chen Cui, Kai Yang, Minjie Lu, Jinhua Huang, Lei Zhang, Fei Li, Jinxi Wang, Biyi Chen, Bin Wang, Duane D. Hall, Zhenwei Pan, Jiang Hong, Long-Sheng Song, Lei Song, Shihua Zhao
Summary: This study identified CIB2 as a unique gene expressed in the atria, which is essential for maintaining atrial structure and function. Loss of CIB2 exacerbates atrial fibrillation occurrence and increases atrial fibrosis, while overexpression of CIB2 decreases atrial fibrillation occurrence and reduces atrial fibrosis. Therefore, CIB2 may represent a potential new target for treating atrial fibrillation.
Review
Oncology
Beatrice S. Ludwig, Horst Kessler, Susanne Kossatz, Ute Reuning
Summary: Integrins have been extensively researched as therapeutic targets in the past few decades, particularly in the field of cancer. Despite clinical trials not meeting expectations yet, integrins remain promising targets due to their expression on diseased cells. To successfully translate integrin-targeted compounds into clinical practice, refined approaches and innovative treatment strategies need to be explored based on accumulated knowledge of integrin biology.
Review
Ophthalmology
Inge Van Hove, Tjing-Tjing Hu, Karen Beets, Tine Van Bergen, Isabelle Etienne, Alan W. Stitt, Elke Vermassen, Jean H. M. Feyen
Summary: Integrins play key roles in eye diseases such as diabetic retinopathy and age-related macular degeneration. Drugs targeting integrin-linked pathways have potential to block disease progression by modulating molecular pathways. In particular, integrins binding to arginyl-glycyl-aspartic acid (ArgGly-Asp; RGD) are involved in disease pathogenesis and therapeutic targets.
PROGRESS IN RETINAL AND EYE RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Xiaolin Yu, Lu Xue, Jing Zhao, Shuhua Zhao, Daqing Wu, Hong Yan Liu
Summary: In this study, a genetically engineered non-cationic non-viral tumor-targeted universal siRNA nanocarrier was developed, which showed great potential for tumor-targeted siRNA delivery with low toxicity and high efficiency. This multifunctional and biomimetic siRNA carrier is biodegradable, suitable for mass production, and holds promise for widespread application in vivo.
Article
Pharmacology & Pharmacy
Barbara Gyuricza, Judit P. Szabo, Viktoria Arato, Daniel Szucs, Adrienn Vagner, Dezso Szikra, Aniko Fekete
Summary: A novel radiotracer for cancer diagnosis containing lactosamine was successfully synthesized, showing high affinity and stability. The compound was labeled with a Ga-68 isotope with high radiochemical purity. This radiopharmaceutical has potential applications in tumor imaging in the future.
Article
Engineering, Biomedical
Kazuro L. Fujimoto, Aika Yamawaki-Ogata, Koichiro Uto, Akihiko Usui, Yuji Narita, Mitsuhiro Ebara
Summary: A novel biodegradable and elastic P(CL-DLLA) copolymer material was developed for RVOT replacement surgery. The material showed enhanced cell infiltration at 8 weeks, complete absorption at 24 weeks, and induced cardiac regeneration at 48 weeks.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Lili Chen, Nanami Fujisawa, Masato Takanohashi, Mazaya Najmina, Koichiro Uto, Mitsuhiro Ebara
Summary: This study demonstrates the rational fabrication of a magnetic composite nanofiber mesh that exhibits distinct hyperthermia, enhanced chemotherapeutic efficiency, and thermo-molecularly targeted therapy for potent therapeutic effects in cancer treatment. The nanofiber system represents a smart approach for localized medication delivery and may offer a new strategy for advanced cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Laszlo Szabo, Xingtao Xu, Koichiro Uto, Joel Henzie, Yusuke Yamauchi, Izumi Ichinose, Mitsuhiro Ebara
Summary: The study focuses on the relationship between the structure and electrochemical performance of porous carbon nanofibers (PCNFs) derived from the biobased building block chitosan. The researchers found that by varying the amount and molecular weight of the sacrificial pore-forming polymer, they could tailor the structure of the PCNFs to have a high surface area and hierarchical pore network, resulting in fast ion diffusion, high specific capacitance, and rate capability. The chitosan-based materials demonstrated high stability and charge efficiency, making them promising for use in charge storage devices.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Erika Yoshihara, Makoto Sasaki, Ahmed Nabil, Michihiro Iijima, Mitsuhiro Ebara
Summary: In this study, a temperature-responsive polymer-protein conjugate was synthesized by introducing a chain transfer agent (CTA) into bovine serum albumin (BSA). The research findings showed that the thermally responsive behavior of the polymer was controlled by the monomer ratio to CTA and the amount of CTA introduced to BSA. Furthermore, a human serum albumin (HSA)-poly(N-isopropylacrylamide) (PNIPAAm) conjugate was synthesized, which could specifically absorb the uremic toxin indoxyl sulfate (IS) and decrease its concentration by thermal precipitation.
Article
Multidisciplinary Sciences
Takahiro Aoyama, Koichiro Uto, Hidetoshi Shimizu, Mitsuhiro Ebara, Tomoki Kitagawa, Hiroyuki Tachibana, Kojiro Suzuki, Takeshi Kodaira
Summary: The newly developed PCL material 4b45/2b20 has a low melting point and high deformation ability, meeting clinically acceptable standards. Compared to commercially available masks, it has a lower elastic modulus and higher elongation at break.
SCIENTIFIC REPORTS
(2021)
Article
Polymer Science
Yasuhiro Nakagawa, Jeonggyu Lee, Yihua Liu, Saed Abbasi, Taehun Hong, Horacio Cabral, Satoshi Uchida, Mitsuhiro Ebara
Summary: This study demonstrates the microglial polarization effect of PtdSer-exposing polymeric particles (PSPs), which can upregulate anti-inflammatory microglia and suppress inflammatory microglia through specific receptor activation. This highlights the potential of PSPs for anti-inflammatory therapy.
Editorial Material
Biotechnology & Applied Microbiology
Saji Uthaman, In-Kyu Park, Kang Moo Huh, James Lai, Mitsuhiro Ebara
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Ailifeire Fulati, Koichiro Uto, Masanobu Iwanaga, Miho Watanabe, Mitsuhiro Ebara
Summary: This article reports a novel shape-memory polymeric string that is ultra-stretchable, strong, tough, and highly recoverable. The contraction force of this string can be customized by adjusting the energy storage capacity, and it displays a controllable shape-memory response time. It has potential applications in blood vessel contraction in minimally invasive surgeries.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Terufumi Yoshida, Masaki Kaibori, Nanami Fujisawa, Mariko Ishizuka, Fusao Sumiyama, Masahiko Hatta, Hisashi Kosaka, Kosuke Matsui, Kensuke Suzuki, Tomoya O. Akama, Tayo Katano, Kengo Yoshii, Mitsuhiro Ebara, Mitsugu Sekimoto
Summary: In this study, lenvatinib sheets incorporating a drug delivery system showed significant antitumor effects in a murine HCC model, inhibiting tumor growth and improving survival rates. The sustained release of lenvatinib from the sheets maintained stable serum levels and effectively suppressed tumor proliferation, indicating their potential as a novel and effective treatment strategy for advanced HCC.
Article
Biotechnology & Applied Microbiology
Lili Chen, Nanami Fujisawa, Masato Takanohashi, Mitsuhiro Ebara
Summary: Researchers developed a smart nanofiber mesh with anticancer abilities, injectability, and fast recovery, which can modulate and control anticancer effects by loading chemotherapeutic drugs and magnetic nanoparticles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mazaya Najmina, Mitsuhiro Ebara, Takahito Ohmura, Koichiro Uto
Summary: This study explores the possibility of promoting the permanent cell cycle arrest of breast cancer cells on a viscoelastic liquid substrate, and highlights the role of rapid bulk stress relaxation in generating reactive oxygen species in semi-3D multicellular aggregates of breast cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
M. Watanabe, M. Okamoto, S. Komichi, H. Huang, S. Matsumoto, K. Moriyama, J. Ohshima, S. Abe, M. Morita, M. Ali, K. Takebe, I. Kozaki, A. Fujimoto, K. Kanie, R. Kato, K. Uto, M. Ebara, A. Yamawaki-Ogata, Y. Narita, Y. Takahashi, M. Hayashi
Summary: This study investigates the effects of functional peptides derived from the S100A8 protein on dental pulp tissue. It found that one of the peptides can promote tertiary dentin formation and inhibit inflammatory responses. These findings provide important insights for the development of next-generation biological vital pulp therapies.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Polymer Science
Ailifeire Fulati, Koichiro Uto, Mitsuhiro Ebara
Summary: This study investigates the effects of crystallinity and crosslinking density on the mechanical properties and shape recovery force of shape-memory polymers (SMPs). The experimental results reveal that the crosslinking density affects the shape recovery force, which is crucial for understanding the energy storage capacity of the polymers.
Article
Chemistry, Multidisciplinary
Yinuo Li, Yoshitaka Matsumoto, Lili Chen, Yu Sugawara, Emiho Oe, Nanami Fujisawa, Mitsuhiro Ebara, Hideyuki Sakurai
Summary: This study proposes a new treatment model for glioblastoma (GBM) using a safe and non-toxic drug-releasing composite Nanofiber mesh (NFM). The NFM combines chemotherapy, molecular targeted therapy, and radiotherapy in a highly simultaneous manner. The combination of temozolomide (TMZ) and 17-allylamino-17-demethoxygeldanamycin (17AAG) in the NFM showed synergistic cytotoxicity and radiation-sensitization effects. The NFM has the potential to address the challenge of high recurrence of GBM post-operatively due to its sustained drug release.
Article
Biochemistry & Molecular Biology
Gyeongwoo Lee, Taishu Iwase, Shunsuke Matsumoto, Ahmed Nabil, Mitsuhiro Ebara
Summary: This study successfully synthesized an antibody-drug conjugate with anti-inflammatory effects and optimized the antibody-polymer ratio. The introduction of p(HEMA-co-MPS) to IgG did not decrease the polymer's anti-inflammatory potential while maintaining its targeting ability. It is suggested to adjust the antibody-polymer ratio appropriately for effective therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
