Article
Chemistry, Analytical
Amirali Selahi, Sanjukta Chakraborty, Mariappan Muthuchamy, David C. Zawieja, Abhishek Jain
Summary: This study demonstrates the coupling of intracellular calcium dynamics between lymphatic endothelial cells (LECs) and muscle cells (LMCs) under pulsatile shear stress. The Lymphangion-Chip platform provides real-time analysis of calcium signaling in LECs and LMCs, revealing the dependence on pulsatile shear rate and the functional consequences of mechanotransduction.
Review
Biochemistry & Molecular Biology
Xin Geng, Yen-Chun Ho, R. Sathish Srinivasan
Summary: The lymphatic vasculature is crucial for maintaining interstitial fluid balance, regulating lipid assimilation, and inflammatory response. Defects in lymphatic vessels or valves can lead to fluid accumulation, metabolic disorders, and inflammation. Studies have identified molecules and mechanical signals that play key roles in the development and function of the lymphatic vasculature, providing insights for potential therapies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Magda N. Hernandez Vasquez, Maria H. Ulvmar, Alejandra Gonzalez-Loyola, Ioannis Kritikos, Ying Sun, Liqun He, Cornelia Halin, Tatiana Petrova, Taija Makinen
Summary: This study identifies FOXP2 as a new flow-induced transcriptional regulator of collecting lymphatic vessel morphogenesis. Loss of FOXP2 results in enlarged collecting vessels and defective valves, highlighting the unique transcription factor codes in establishing vessel-type-specific endothelial cell identities.
Article
Biochemistry & Molecular Biology
Eleftheria Michalaki, Vinay N. Surya, Mariana Rodriguez-Hakim, Gerald G. Fuller, Alexander R. Dunn
Summary: This study demonstrates that the flow environment within lymphatic valves can activate key regulatory pathways involved in valve maintenance, as evidenced by the upregulation of gene transcription and calcium signaling in response to spatial and temporal variations in wall shear stresses.
Article
Cell Biology
Fengchan Li, Kunmin Yan, Lili Wu, Zhong Zheng, Yun Du, Ziting Liu, Luyao Zhao, Wei Li, Yulan Sheng, Lijie Ren, Chaojun Tang, Li Zhu
Summary: The study showed that disturbed blood flow can alter arterial wall cells, increasing the risk of atherosclerosis. Single-cell RNA sequencing revealed multiple cell subpopulations related to disturbed blood flow, including mechanosensitive endothelial cells and smooth muscle cells potentially impacting arterial stiffness.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Raghu P. Kataru, Jung Eun Baik, Hyeung Ju Park, Catherine L. Ly, Jinyeon Shin, Noa Schwartz, Theresa T. Lu, Sagrario Ortega, Babak J. Mehrara
Summary: The study shows that intracellular modulation of the VEGF-C signaling pathway can induce the formation of functional lymphatic networks without off-target effects and inflammation.
Review
Physiology
Tomohiro Shiiya, Masanori Hirashima
Summary: During development, lymphatic endothelial cell (LEC) progenitors differentiate from venous endothelial cells only in limited regions of the body. LEC migration and subsequent tube formation are essential processes for the development of tubular lymphatic vascular network throughout the body. This review discusses the chemotactic factors, LEC-extracellular matrix interactions, and planar cell polarity regulating LEC migration and formation of tubular lymphatic vessels, providing insights into the molecular mechanisms underlying physiological lymphatic vascular development and lymphangiogenesis associated with pathological conditions.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Immunology
Masayuki Miyasaka
Summary: Recent studies have shown that lymphatic endothelial cells (LECs) consist of multiple cell subsets expressing unique sets of genes in distinct locations in the body, with both conserved and species-specific gene expression patterns. However, the adaptive nature of this heterogeneity in response to the external environment and the transcriptional and regulatory pathways programming the different LEC subsets remain unclear.
INFLAMMATION AND REGENERATION
(2021)
Article
Biochemistry & Molecular Biology
Ahmad Chehaitly, Anne-Laure Guihot, Coralyne Proux, Linda Grimaud, Jade Aurriere, Benoit Legouriellec, Jordan Rivron, Emilie Vessieres, Clement Tetaud, Antonio Zorzano, Vincent Procaccio, Francoise Joubaud, Pascal Reynier, Guy Lenaers, Laurent Loufrani, Daniel Henrion
Summary: The reduction in mitochondrial fusion in mouse endothelial cells was found to impair the dilator response to shear stress, leading to excessive superoxide production and promoting greater atherosclerosis development.
Article
Biochemistry & Molecular Biology
Marta Turati, Gianluca Mattei, Alessia Boaretto, Alberto Magi, Maura Calvani, Roberto Ronca
Summary: The lymphatic vascular system plays a crucial role in cancer progression by activating lymphatic endothelial cells and facilitating the formation of new lymphatic vessels. This study revealed that cancer-derived and microenvironmental mediators activate specific molecular pathways in lymphatic endothelial cells, leading to endothelial activation, morphogenesis, and cytokine-mediated signaling. The findings provide new insights into the lymphangiogenesis process and its relevance in different types of tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chak Kwong Cheng, Xiao Lin, Yujie Pu, Joyce Ka Yu Tse, Yu Wang, Cheng-Lin Zhang, Xiaoyun Cao, Chi Wai Lau, Juan Huang, Lei He, Jiang-Yun Luo, Yu-Tsung Shih, Song Wan, Chi Fai Ng, Li Wang, Ronald Ching Wan, Jeng-Jiann Chiu, Ting Fung Chan, Xiao Yu Tian, Yu Huang
Summary: This study utilized single-cell RNA sequencing to explore the transcriptional landscape and heterogeneity of dysfunctional endothelial cells in atherosclerotic aortae. SOX4 was identified as a novel marker for atherosclerosis and was shown to promote atherogenesis and endothelial-to-mesenchymal transition. Hyperlipidemia-associated cytokines and oscillatory blood flow were found to upregulate SOX4 expression, while the anti-diabetic drug metformin suppressed SOX4 levels in endothelial cells.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Food Science & Technology
Yeonju Ko, Eun-Hye Kim, Donghyun Kim, Sungbin Choi, Junkyung Gil, Han Jin Park, Yusun Shin, Wondong Kim, Ok-Nam Bae
Summary: This study found that parabens, commonly used as preservatives, can induce morphological and functional changes in red blood cells (RBCs), leading to an increased risk of blood clot formation.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Wenji Piao, Lushen Li, Vikas Saxena, Jegan Iyyathurai, Ram Lakhan, Yigang Zhang, Isadora Tadeval Lape, Christina Paluskievicz, Keli L. Hippen, Young Lee, Emma Silverman, Marina W. Shirkey, Leonardo Riella, Bruce R. Blazar, Jonathan S. Bromberg
Summary: This study reveals the regulatory role of PD-L1 signaling in the transendothelial migration of T cells and highlights the importance of PD-L1 in cell migration and immune regulation.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Kiho Son, Amer Hussain, Roma Sehmi, Luke Janssen
Summary: Mechanosensitive properties of eosinophil integrins allow for detection of fluid shear stress, triggering intracellular calcium release and cytoskeletal reorganization. Pharmacological inhibitors targeting calcium signaling and actin polymerization pathways affect shear stress-induced migration of eosinophils, impacting their ability to move across surfaces.
Article
Cell Biology
Matthew Herrera, Patricia Molina, Flavia M. Souza-Smith
Summary: Chronic alcohol consumption increases susceptibility to inflammation, bacterial, and viral infections, while alcohol-induced lymphatic leakage may be attributed to disruption of junctional protein complexes in lymphatic endothelial cells. Phosphorylated p38 may play a key role in regulating alcohol-induced lymphatic endothelial cell permeability.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Mechanics
James E. Moore, Christopher D. Bertram
ANNUAL REVIEW OF FLUID MECHANICS, VOL 50
(2018)
Article
Biology
Hojjat Bazzazi, Yu Zhang, Mohammad Jafarnejad, Aleksander S. Popel
JOURNAL OF THEORETICAL BIOLOGY
(2018)
Article
Physiology
Hojjat Bazzazi, Yu Zhang, Mohammad Jafarnejad, Jeffrey S. Isenberg, Brian H. Annex, Aleksander S. Popel
FRONTIERS IN PHYSIOLOGY
(2018)
Article
Pharmacology & Pharmacy
Mohammad Jafarnejad, Chang Gong, Edward Gabrielson, Imke H. Bartelink, Paolo Vicini, Bing Wang, Rajesh Narwal, Lorin Roskos, Aleksander S. Popel
Article
Immunology
David C. Zawieja, Sangeetha Thangaswamy, Wei Wang, Raquel Furtado, Cristina C. Clement, Zachary Papadopoulos, Marco Vigano, Eric A. Bridenbaugh, Lello Zolla, Anatoliy A. Gashev, Jonathan Kipnis, Gregoire Lauvau, Laura Santambrogio
JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Oleg Milberg, Chang Gong, Mohammad Jafarnejad, Imke H. Bartelink, Bing Wang, Paolo Vicini, Rajesh Narwal, Lorin Roskos, Aleksander S. Popel
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
M. Jafarnejad, A. Z. Ismail, D. Duarte, C. Vyas, A. Ghahramani, D. C. Zawieja, C. Lo Celso, G. Poologasundarampillai, J. E. Moore
SCIENTIFIC REPORTS
(2019)
Editorial Material
Oncology
Qian Xu, James E. Moore
JOURNAL OF PATHOLOGY
(2020)
Article
Multidisciplinary Sciences
Sarah C. Johnson, Sanjukta Chakraborty, Anastasios Drosou, Paula Cunnea, Dimitrios Tzovaras, Katherine Nixon, David C. Zawieja, Mariappan Muthuchamy, Christina Fotopoulou, James E. Moore
Article
Biophysics
Christopher J. Morris, David C. Zawieja, James E. Moore
Summary: The study developed a multiscale computational model to simulate phasic and tonic contractions in lymphatic muscle and coupled it with a lymphangion pumping model to investigate lymphedema treatment. By relating model results to experiments and showing similarities with rat experiments, it provided initial estimates of lymphatic muscle contraction energetics.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Willy V. Bonneuil, Daniel J. Watson, Jennifer Frattolin, Matthew J. Russell, Francesca Fasanella Masci, Mikaila Bandara, Bindi S. Brook, Robert J. B. Nibbs, James E. Moore
Summary: This study presents a microfluidic chip that can generate precisely controlled chemotactic gradients under advective-diffusive conditions. By using torque-actuated membranes and modifying the resistance of fluid channels, stable gradients and constant interstitial flow can be achieved. This method is important for studying the formation and maintenance of molecular gradients and cell migration under physiological conditions.
Article
Multidisciplinary Sciences
Sarah C. Johnson, Jennifer Frattolin, Lowell T. Edgar, Mohammad Jafarnejad, James E. Moore
Summary: The research indicates that swelling in lymph nodes can enhance the activation of T cells, but early occurrence of swelling or low TC cognate frequency can reduce CD8(+) T cell production. Temporarily extending retention of effector T cells can mitigate the negative effects of swelling.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2021)
Article
Mathematical & Computational Biology
Mohammad Jafarnejad, Richard J. Sove, Ludmila Danilova, Adam C. Mirando, Yu Zhang, Mark Yarchoan, Phuoc T. Tran, Niranjan B. Pandey, Elana J. Fertig, Aleksander S. Popel
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2019)
Review
Engineering, Biomedical
James E. Moore, Bindi S. Brook, Robert J. B. Nibbs
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2018)