期刊
ACS APPLIED MATERIALS & INTERFACES
卷 5, 期 14, 页码 6494-6501出版社
AMER CHEMICAL SOC
DOI: 10.1021/am401935y
关键词
hybrid nanomaterials; silica nanoparticles; gold nanopartides; caspase-3; fluorescence resonance energy transfer
资金
- National Scientific Foundation of China [NSFC 21171038, 31100723]
- Guangdong Natural Science Foundation [S2011040001778]
- Funding Scheme of Guangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instruments [2011A060901013]
- Research Grants Council of the Hong Kong Special Administrative Region [CityU161310]
Recent developments in the rational design and the controlled assembly of nanoscale building blocks have resulted in functional devices such as nano-optoelectronics, novel contrast probes for molecular imaging, and nanosensors. In the present study, we designed and synthesized a hybrid nanomaterial consisting of [Ru(bpy)(3)](2+)-encapsulated silica nanoparticles (SiNPs) and gold nanoparticles (AuNPs) through peptide-bridged assembly in a controllable way. A peptide that contains recognition sequence DEVD specific for active caspase-3 cleavage was employed to bring SiNPs and AuNPs into close proximity through specific molecular recognition. A FRET system with SiNPs as energy donors and AuNPs as energy acceptors has been thus developed and applied for caspase-3 detection. A change in distance between the two building blocks resulted in a change in FRET efficiency, causing a ratiometric change in emission. Caspase-3 triggers the cleavage of the peptide links between the two nanoparticles and releases AuNPs from the nanohybrids, inducing the activation of SiNPs to the ON state. The fluorescence turn-on response is specific to caspase-3 and allows the detection of caspase-3 as low as 0.05 U mL(-1) (similar to 6 pM).
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