Review
Chemistry, Analytical
Reza Eivazzadeh-Keihan, Zahra Saadatidizaji, Ali Maleki, Miguel de la Guardia, Mohammad Mahdavi, Sajjad Barzegar, Samad Ahadian
Summary: Thrombin is a serine protease that plays a crucial role in blood clotting and homeostasis. Detecting thrombin quickly and accurately is important for biological analysis and clinical diagnosis. Various biosensors have been developed to achieve highly selective and specific detection of thrombin. Aptasensors, which use DNA or RNA aptamers as biological recognition elements, have shown great sensitivity and affinity in identifying thrombin.
Article
Chemistry, Analytical
Houman Moteshareie, Walid M. Hassen, Jonathan Vermette, Jan J. Dubowski, Azam F. Tayabali
Summary: The study investigated the sensitivity and selectivity of commercially available detection reagents for Bt spores, as well as the functionalization of GaAs surface for photonic biosensing. The results demonstrate that the selected aptamer and llama rAb show high efficiency in capturing Bt spores on the surface of GaAs, indicating their potential for developing GaAs-based biosensing devices.
Article
Chemistry, Multidisciplinary
Man Zhu, Fan Xu, Siyuan Miao, Chongyu Xie, Hui Li, Shaoguang Li, Fan Xia
Summary: This study reports the design of an E-AB biosensor with dual recognition sites for rapid detection of thrombin. The biosensor showed faster response time and good performance in terms of dynamic range and specificity.
Article
Food Science & Technology
Han Du, Tao Ping, Wei Wu, Qingli Yang
Summary: This study prepared a simple and sensitive DNA tetrahedral fluorescence signal amplifier for sandwich detection of Staphylococcus aureus. The amplifier improved the fluorescence detection sensitivity and selectivity of S. aureus, and it may also be applicable to the detection of other food pathogens or environmental microorganisms in the future.
Article
Nanoscience & Nanotechnology
Kamila Malecka, Elena E. Ferapontova
Summary: Thrombin is a central biomarker in cardiovascular diseases and Alzheimer's disease development, but its electroanalysis is complicated by low physiological concentrations and nonspecific binding. A new electrochemical method was developed to detect femtomolar levels of thrombin in serum and CSF using G4-hemin DNAzyme, which is simple, sensitive, and selective, providing a new tool for ultrasensitive electrocatalytic assays.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Environmental
Qian Zhu, Lanhua Liu, Ruoyu Wang, Xiaohong Zhou
Summary: A sensitive EWF biosensor for STR detection using SPA has been developed, showing good selectivity and reuse capability. The detection steps are simple and the recovery rate for water samples is high.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Analytical
Jing Xu, Yinbing Liu, Ke-Jing Huang, Yang-Yang Hou, Xiaoxuan Sun, Jiaqiang Li
Summary: Realization of a highly sensitive analysis and sensing platform is crucial for early-stage tumor diagnosis. In this study, a self-powered biosensor with a novel sandwich graphdiyne (SGDY) structure combined with an aptamer-specific recognition function was developed to sensitively and accurately detect tumor markers.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Dharmendra Neupane, Keith J. Stine
Summary: The development of sensitive and selective assays for protein biomarkers and other biological analytes is crucial for advancements in clinical diagnostics and bioanalytical chemistry. The use of aptamers in electrochemical sandwich assays is increasingly recognized for its potential advantages, such as working with different partners like antibodies, lectins, or nanomaterials. Impressive progress has been made in electrochemical sandwich assays, with the use of nanoparticles as carriers for detection partners and nanostructured surfaces to increase surface area and improve electron transfer.
APPLIED SCIENCES-BASEL
(2021)
Article
Biophysics
Lei Huang, Shulin Tian, Wenhao Zhao, Ke Liu, Xing Ma, Jinhong Guo
Summary: The lateral flow assay (LFA) is a widely used paper-based on-site biosensor for detecting target analytes in minutes. Aptamers, as biorecognition molecules, are being explored as an alternative to antibodies in LFAs, offering advantages over traditional methods. Different formats of aptamer-based LFAs can achieve on-site detection of target analytes efficiently.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Medicine, Research & Experimental
Tao Wang, Lanmei Chen, Arpitha Chikkanna, Suxiang Chen, Isabell Brusius, Nabayet Sbuh, Rakesh N. Veedu
Summary: This study analyzes key issues in the design of aptamer-based LFAs, including immobilization strategies, signaling methods, and target capturing approaches, providing a comprehensive overview of aptamer-based LFA design strategies to facilitate the development of optimized aptamer-based LFAs by researchers.
Article
Biophysics
Anabel Villalonga, Itziar Estabiel, Ana M. Perez-Calabuig, Beatriz Mayol, Concepcion Parrado, Reynaldo Villalonga
Summary: The novel amperometric aptasensor constructed for the specific detection of cardiac troponin I (cTnI) showed high sensitivity, specificity, and wide applicability for detecting the cardiac biomarker in a broad concentration range. It utilized a sandwich-type structure for detection and provided a detection limit of 0.6 pg/mL, along with high reproducibility and stability for quantifying cTnI in reconstituted human serum samples.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Feng Qiu, Xiaoyan Gan, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: This study presents a novel method for sensitive detection of thrombin in human serums. By utilizing target-promoted magnetic nanoparticle-decorated DNA polymers for proximity binding hybridization chain reaction, the detection of thrombin is significantly enhanced.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Jing Jiang, Yuanyuan Wang, Xianwen Kan
Summary: A label-free and ultra-sensitive sensing strategy for colorimetric detection of thrombin was developed using a catechol oxidase-mimicking nanozyme, MOF-818. The nanozyme composite showed high catalytic activity and could effectively recognize and bind thrombin, resulting in accurate detection with a low limit of detection. This strategy has great potential for medical diagnosis and human health treatment.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Multidisciplinary Sciences
Carlotta Peruzzi, Silvia Battistoni, Daniela Montesarchio, Matteo Cocuzza, Simone Luigi Marasso, Alessio Verna, Laura Pasquardini, Roberto Verucchi, Lucrezia Aversa, Victor Erokhin, Pasquale D'Angelo, Salvatore Iannotta
Summary: The novel gate electrode composed of graphene, gold nanoparticles, and aptamers achieves high selectivity and sensitivity in a biosensor, with a limit of detection in the picomolar range. The hierarchical organization reduces steric hindrance and false positives, making it suitable for various biomedical applications.
SCIENTIFIC REPORTS
(2021)
Article
Materials Science, Biomaterials
Kristina Ditte, Trang Anh Nguyen Le, Oliver Ditzer, Diana Isabel Sandoval Bojorquez, Soosang Chae, Michael Bachmann, Larysa Baraban, Franziska Lissel
Summary: During the COVID-19 pandemic, a quick and effective solution is required for fast screening of large groups. Researchers have developed an organic field-effect transistor (OFET)-based biosensing device that can detect both SARS-CoV-2 antigens and anti-SARS-CoV-2 antibodies in less than 20 minutes. The device utilizes a stretchable and semiconducting triblock copolymer (TBC) film functionalized with specific antibodies or receptor-binding domains for the detection of the virus and antibodies. This innovative biosensor has high sensitivity and the potential for low-cost wearable or on-skin diagnostic platforms.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemical Research Methods
Xuan Peng, Zeljko Janicijevic, Sandy Lemm, Markus Laube, Jens Pietzsch, Michael Bachmann, Larysa Baraban
Summary: The functional interaction between cancer cells and the surrounding microenvironment is not well understood, leading to the development of various in vitro tumor models. Factors such as nutrient transport, space availability, and confinement affect the size, shape, and metabolism of tumoroids. A low-cost method based on fluidics is presented to generate alginate and alginate-chitosan microcapsules for growing human hepatoma (HepG2) spheroids of different dimensions and geometries. The composition and thickness of the hydrogel shell are selectively tuned to control the permeability of the microcapsules. The diffusion of benchmark molecules through the shell is systematically investigated using experiments and simulations, ensuring efficient mass transfer and filtering of biochemical species. The metabolic activity of spheroids in microcapsules is confirmed through chromatography studies. Different phenotypic 3D cell assemblies are observed inside capsules based on available space, varying in cell aggregation tightness and shape. Overall, the system with tunable shell thickness and permeability provides a promising platform for studying cancer spheroid formation and their interaction with the surrounding microenvironment.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Electrical & Electronic
Waltenegus Dargie, Jianjun Wen, Luis Antonio Panes-Ruiz, Leif Riemenschneider, Bergoi Ibarlucea, Gianaurelio Cuniberti
Summary: Human beings live and work in close proximity to dangerous gases, which can cause considerable damages to human lives and properties, as well as have high impact on the environment. Hence, diligent monitoring and management of these gases are of profound importance.
IEEE SENSORS JOURNAL
(2023)
Article
Computer Science, Artificial Intelligence
Tianfei Zhou, Fatih Porikli, David J. Crandall, Luc Van Gool, Wenguan Wang
Summary: Video segmentation is crucial in various practical applications such as enhancing visual effects in movies, understanding scenes in autonomous driving, and creating virtual background in video conferencing. Deep learning-based approaches have shown promising performance in video segmentation. This survey comprehensively reviews two main research lines - generic object segmentation and video semantic segmentation - by introducing their task settings, background concepts, need, development history, and challenges. Representative literature and datasets are also discussed, and the reviewed methods are benchmarked on well-known datasets. Open issues and opportunities for further research are identified, and a public website is provided to track developments in this field: https://github.com/tfzhou/VS-Survey.
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE
(2023)
Article
Chemistry, Multidisciplinary
Diana Isabel Sandoval Bojorquez, Zeljko Janicijevic, Brenda Palestina Romero, Eduardo Sergio Oliveros Mata, Markus Laube, Anja Feldmann, Alexandra Kegler, Laura Drewitz, Ciaran Fowley, Jens Pietzsch, Juergen Fassbender, Torsten Tonn, Michael Bachmann, Larysa Baraban
Summary: Detection of COVID-19-associated antigens and respective antibodies is achieved using an interdigitated gold nanowire-based impedance nanobiosensor. The sensor devices demonstrate high sensitivity and a low limit of detection for the antibodies and virus antigen. Clinical plasma samples analysis shows the applicability of this platform in determining the infection or immunity status of patients.
Article
Construction & Building Technology
Jitong Zhao, George Karalis, Marco Liebscher, Lazaros Tzounis, Thomas Koeberle, Dieter Fischer, Frank Simon, Muhannad Al Aiti, Gianaurelio Cuniberti, Viktor Mechtcherine
Summary: This proof-of-concept study demonstrates the fabrication of a multifunctional reinforcing grid-building material within a thermoelectric element generator configuration. Carbon fiber yarns were impregnated with a geopolymer-based suspension and tested for their properties. The resulting hardened mineral-impregnated carbon-fiber reinforcements were used as thermoelements to assemble a grid-like TEG, which generated power upon exposure to a temperature difference.
ENERGY AND BUILDINGS
(2023)
Article
Chemistry, Physical
Neeraj Bangruwa, Mayra Suryansh, Mayra Peralta, Rafael Gutierrez, Gianaurelio Cuniberti, Debabrata Mishra
Summary: In this study, we investigate the sequence-dependent chiral-induced spin selectivity (CISS) in double-stranded DNA. By using time-correlated single-photon counting and electrochemical impedance spectroscopy, we find that the average lifetime of photo-excited electrons is influenced by the CISS effect generated by the DNA molecule. The observed experimental trends are supported by simulations using a tight binding method.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Mayra Peralta, Steven Feijoo, Solmar Varela, Rafael Gutierrez, Gianaurelio Cuniberti, Vladimiro Mujica, Ernesto Medina
Summary: We investigate the role of electron-spin-phonon coupling in DNA using an effective model Hamiltonian and its connection to the Chiral-Induced Spin Selectivity (CISS) effect. The semiclassical electron transfer in a tight-binding model of DNA with intrinsic spin-orbit coupling is described using the envelope function approach. We find that only acoustic modes exhibit spin-phonon coupling, while optical modes show electron-phonon interaction without spin coupling. An effective Hamiltonian is derived, in which the eigenstates carry spin currents protected by spin-inactive stretching optical modes. The stronger interaction between optical phonons and electrons allows for the decoherence effects and the spin filtering effects observed in CISS.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Mohammad Khazaei, Soungmin Bae, Rasoul Khaledialidusti, Ahmad Ranjbar, Hannu-Pekka Komsa, Somayeh Khazaei, Mohammad Bagheri, Vei Wang, Yasuhide Mochizuki, Mitsuaki Kawamura, Gianaurelio Cuniberti, S. Mehdi Vaez Allaei, Kaoru Ohno, Hideo Hosono, Hannes Raebiger
Summary: MAX phases are layered non-van der Waals materials composed of stacking hexagonal layers of transition metal (M), a group III-VI element(A), and carbon or nitrogen (X). Through high-throughput calculations, 761 dynamically stable MAX phases have been identified, with 466 structures potentially synthesizable. To discover new structural phases, 361 MAX systems with dynamical instabilities were investigated, leading to the discovery of novel superlattices by reconstructing the triangular lattice of A-atoms into 0D-clusters, quasi-1D-chains, or creating 2D Haeckelite or Kagome-like lattices. This work introduces a new approach to discovering novel MAX phases from conventional structures without element alloying.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Physical
Alvaro Rodriguez Mendez, Arezoo Dianat, Leonardo Medrano Sandonas, Rafael Gutierrez, Gianaurelio Cuniberti
Summary: In this study, the electronic properties of phosphorene monolayers functionalized with hydroxyl and cysteine molecules were investigated using spin-polarized density-functional theory. It was found that functionalization not only induced electronic states within the semiconducting gap, but also resulted in local magnetism. The ferro- or anti-ferromagnetic ground states could be obtained depending on molecular coverage, lattice direction, and molecular chirality. Functionalized phosphorene monolayers were proposed as bipolar magnetic semiconductors based on the obtained spin-dependent band structures. The electronic parameters used to characterize bipolar magnetic semiconductors became increasingly distinct with increasing surface coverage, suggesting a possible route for designing such materials via targeted molecular functionalization.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Review
Chemistry, Analytical
Alexandra Parichenko, Shirong Huang, Jinbo Pang, Bergoi Ibarlucea, Gianaurelio Cuniberti
Summary: Inspired by biological noses, e-noses imitate them by using gas sensor arrays to detect and identify surrounding gases and volatile compounds. Two-dimensional materials have shown remarkable sensitivity at room temperature, addressing energy efficiency and sensitivity issues. This review highlights advancements in the development of e-noses, including transduction mechanisms and deposition methods for two-dimensional materials. Artificial intelligence tools are discussed for smart data analysis to overcome selectivity limitations.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Suchetana Sarkar, Kwan Ho Au-Yeung, Tim Kuehne, Albrecht Waentig, Dmitry A. Ryndyk, Thomas Heine, Gianaurelio Cuniberti, Xinliang Feng, Francesca Moresco
Summary: We investigated a prochiral thiophene-based molecule that self-assembles on the Au(111) surface forming islands with different domains using low temperature scanning tunneling microscopy. Two different conformations of the single molecule were observed in the domains, depending on the rotation of bromothiophene groups. By applying voltage pulses from the tip, single molecules could be switched between the two conformations. Scanning tunneling spectroscopy revealed that electronic resonances were mainly localized at the same positions in both conformations. Density-functional theory calculations supported the experimental findings. Moreover, we found that on Ag(111), only one configuration was present and the switching effect was suppressed.
SCIENTIFIC REPORTS
(2023)
Article
Computer Science, Artificial Intelligence
Wenqi Wang, Run Wang, Lina Wang, Zhibo Wang, Aoshuang Ye
Summary: Deep neural networks have achieved remarkable success in various tasks, but they are vulnerable to adversarial examples in both image and text domains. Adversarial examples in the text domain can evade DNN-based text analyzers and pose threats to the spread of disinformation. This paper comprehensively surveys the existing studies on adversarial techniques for generating adversarial texts and the corresponding defense methods, aiming to inspire future research in developing robust DNN-based text analyzers against known and unknown adversarial techniques.
IEEE TRANSACTIONS ON KNOWLEDGE AND DATA ENGINEERING
(2023)
Article
Chemistry, Physical
Vyacheslav R. Misko, Larysa Baraban, Denys Makarov, Tao Huang, Pierre Gelin, Ileana Mateizel, Koen Wouters, Neelke De Munck, Franco Nori, Wim De Malsche
Summary: This study proposes a novel method for selecting active species based on their motility using an acoustofluidic setup. Highly motile species can escape from the acoustic trap, making this method suitable for selecting active species like sperm for medically assisted reproduction. The proposed method is more flexible and can also be applied to other active systems.
Article
Biophysics
Zeljko Janicijevic, Trang-Anh Nguyen-Le, Ahmed Alsadig, Isli Cela, Rugile Zilenaite, Taufhik Hossain Tonmoy, Manja Kubeil, Michael Bachmann, Larysa Baraban
Summary: The study presents a portable multiplexed biosensor platform based on the extended gate field-effect transistor and demonstrates its amplified response using gold nanoparticle-based bioconjugates. The platform achieves sensitivities comparable to fully customized nanofabricated potentiometric sensors and shows a significant amplification of the potentiometric response using gold nanoparticle bioconjugation. The results highlight the potential of the biosensor for multidimensional diagnostics in clinical and laboratory settings.
BIOSENSORS & BIOELECTRONICS
(2023)