Article
Biochemistry & Molecular Biology
Wei Ou, Yu Liang, Yu Qing, Wei Wu, Maodi Xie, Yabing Zhang, Yarong Zhang, Liwei Ji, Haiyang Yu, Tao Li
Summary: The study revealed that hypoxic acclimation (HA) can alleviate cardiac ischemia-reperfusion (I/R) injury by enhancing antioxidative capacity through activation of glucose metabolism, particularly by upregulating the enzyme G6PDH. This cardioprotective effect highlights the potential of HA as a promising strategy against I/R injury, suggesting that O-GlcNAc modification of G6PDH could be a therapeutic target for ischemic heart disease.
Article
Biochemistry & Molecular Biology
Austin C. Boese, Sumin Kang
Summary: Cancer cells exhibit abnormal metabolic activity, often preferring aerobic glycolysis, and mitochondrial metabolic pathways are also reprogrammed in cancer. Cancer cells have higher levels of reactive oxygen species compared to non-cancerous cells and must employ diverse metabolic strategies to prevent oxidative stress.
Article
Biochemistry & Molecular Biology
Qifei Wang, Pu Wang, Zelian Qin, Xin Yang, Bailin Pan, Fangfei Nie, Hongsen Bi
Summary: Keloids exhibit enhanced glycolysis and attenuated oxidative phosphorylation under hypoxic conditions. The upregulation of HIF1 alpha and PI3K/AKT signaling pathways was observed in keloid fibroblasts. Overall, hypoxia promoted cell proliferation, migration, invasion, and collagen synthesis while inhibiting apoptosis in KFB.
Review
Cell Biology
Danilo Cucchi, Amy Gibson, Sarah A. Martin
Summary: The DNA damage response (DDR) and cellular metabolism are closely related, with metabolic pathways influencing DDR repair process and DDR proteins regulating metabolic flux. This connection holds great importance in cancer biology.
Article
Cell Biology
Jie Li, Cuimiao Zheng, Qiuwen Mai, Xi Huang, Wenfeng Pan, Jingyi Lu, Zhengfan Chen, Suman Zhang, Chunyu Zhang, Hua Huang, Yangyang Chen, Hongbo Guo, Zhenyin Wu, Chunnuan Deng, Yiting Jiang, Bo Li, Junxiu Liu, Shuzhong Yao, Chaoyun Pan
Summary: The depletion of FAH reduces chemosensitivity in epithelial ovarian cancer. Under genotoxic chemotherapy, FAH is oxidized and translocates to the nucleus, where it suppresses translesion DNA synthesis and improves chemosensitivity. Tyrosine supplementation can enhance sensitivity to genotoxic chemotherapeutics.
Review
Biochemistry & Molecular Biology
Robert T. Mallet, Johannes Burtscher, Vincent Pialoux, Qadar Pasha, Yasmin Ahmad, Gregoire P. Millet, Martin Burtscher
Summary: High-altitude illnesses result from acute exposure to high altitude/hypoxia. Understanding molecular mechanisms is essential for optimizing hypoxic acclimatization for efficient prophylaxis and treatment of these illnesses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Jay Jethva, Romy R. Schmidt, Margret Sauter, Jennifer Selinski
Summary: This review discusses the mechanisms by which plants adapt to low-oxygen environments and survive re-oxygenation, focusing on mitochondrial retrograde signaling, chromatin modification, and mitochondrial alternative electron transport pathways.
Article
Biochemistry & Molecular Biology
Nadine Abdel Hadi, Emeline Boet, Airelle Lahalle, Laura Lauture, Alice Refeyton, Gabriela Reyes-Castellanos, Nathalie Caplet, Alice Carrier, Laurent Le Cam, Nathalie M. Mazure, Jean-Ehrland Ricci, Stephane Rocchi, Jean-Emmanuel Sarry, Sophie Vasseur, Marija Vlaski-Lafarge, Rodrigue Rossignol, Frederic Bost
Summary: The 4th International Metabolism and Cancer meeting was originally scheduled to take place in Bordeaux, France, but was held virtually with around 600 participants from 47 countries attending daily. The meeting featured 21 speakers, including Nobel Prize winner Sir Peter J. Ratcliffe, and covered topics such as redox and energy metabolism, hypoxia, metabolic profiling, epigenetic control, signaling, and cancer metabolism. A summary of the scientific highlights of the event is provided in this report.
Review
Cell Biology
Matthew Hunt, Monica Torres, Etty Bachar-Wikstrom, Jakob D. Wikstrom
Summary: Mitochondria play a critical role in wound healing and chronic wound pathogenesis through various mechanisms such as metabolism, apoptosis, and redox signaling. However, most studies have used animal models instead of patient tissue, highlighting a need for more research using human samples.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Haojian Li, Yue Liu, Yunjie Xiao, Crystal N. Wilson, Hui Jen Bai, Maxwell D. Jones, Shihchun Wang, Jennie E. DeVore, Esther Y. Maier, Stephen T. Durant, Myriem Boufraqech, Urbain Weyemi
Summary: Cancer treatments targeting DNA repair deficiencies often encounter drug resistance, likely due to alternative metabolic pathways that counteract the most damaging effects. In this study, we identified KEAP1 as a key factor that desensitizes cancer cells to ATM inhibition, leading to increased sensitivity to DNA damage. Moreover, we found a negative correlation between ATM levels and KEAP1 levels in multiple solid malignancies, suggesting the potential of targeting ATM and KEAP1 as vulnerabilities in solid tumors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemical Research Methods
Stefan Rudloff, Andrea Bileck, Lukas Janker, Nicola Wanner, Nastassia Liaukouskaya, Carsten Lundby, Tobias B. Huber, Christopher Gerner, Uyen Huynh-Do
Summary: This research focuses on the response of fetal kidney to chronic hypoxia and explores the key events that lead to accelerated aging in chronic hypoxic human diseases. The study reveals a dichotomous response in fetal kidneys, with both cellular adaptations for survival and processes inducing a senescence-like phenotype. Additionally, the expression of antiaging proteins is reduced under chronic hypoxia. These findings provide a solid foundation for the hypothesis of fetal programming of adult diseases and offer potential biomarkers for detecting and targeting accelerated aging in chronic hypoxic human diseases.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Review
Immunology
Yubo Li, Chengming Li, Tao Luo, Tian Yue, Wenjing Xiao, Ling Yang, Zaiyuan Zhang, Fei Han, Pan Long, Yonghe Hu
Summary: With the increasing participation of low-altitude individuals in high-altitude activities, the study of high altitude cerebral edema (HACE) has regained attention. HACE is a severe acute mountain sickness that occurs due to hypobaric hypoxia at high altitudes and is characterized by consciousness disturbance and ataxia. Previous research has suggested that the pathogenesis of HACE may involve cerebral blood flow disorders, blood-brain barrier disruption, and brain parenchymal cell injury caused by inflammatory factors. Recent studies have also shown that the imbalance of REDOX homeostasis is involved in HACE, resulting in abnormal activation of microglia and disruption of tight junctions in vascular endothelial cells through excessive production of mitochondrial-related reactive oxygen species. Therefore, this review focuses on the role of REDOX homeostasis and its potential in HACE treatment, which is of great importance for expanding the understanding of HACE pathogenesis and exploring possible therapeutic approaches targeting REDOX homeostasis.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Endocrinology & Metabolism
Kerri L. M. Smith, Agnieszka Swiderska, Mitchell C. Lock, Lucia Graham, Wulan Iswari, Tashi Choudhary, Donna Thomas, Hager M. Kowash, Michelle Desforges, Elizabeth C. Cottrell, Andrew W. Trafford, Dino A. Giussani, Gina L. J. Galli
Summary: Insufficient oxygen supply during fetal development affects fetal cardiac mitochondrial respiration in a sex-dependent manner. Maternal antioxidant treatments do not provide similar protection to cardiac mitochondria in male and female fetuses.
JOURNAL OF PINEAL RESEARCH
(2022)
Article
Environmental Sciences
Priyanka Prasad, Sushant Kumar Singh, Sukanya Ghosh, Suchisnigdha Dutta, Dona Sinha
Summary: This study investigated the impact of different exposure levels of arsenic in groundwater on cellular redox status, DNA damage, and repair mechanisms in rural women of West Bengal. The results showed a dose-dependent relationship between arsenic exposure levels and oxidative stress, DNA damage, and suppression of DNA repair proteins. Additionally, the exposed population had a very high risk of cancer.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Rami Mosaoa, Anna Kasprzyk-Pawelec, Harvey R. Fernandez, Maria Laura Avantaggiati
Summary: The mitochondrial citrate/isocitrate carrier (CIC) plays a crucial role in human diseases by facilitating the transport of ions and metabolites through the mitochondrial membrane, impacting mitochondrial metabolism and respiration. Additionally, CIC also serves various fundamental activities in the cytosol, such as acting as a metabolic substrate, an allosteric enzymatic regulator, and an epigenetic modifier.
Article
Oncology
Alina Wittka, Julia Ketteler, Lars Borgards, Patrick Maier, Carsten Herskind, Verena Jendrossek, Diana Klein
Summary: In prostate cancer, inhibiting the gain of CAV1 in malignant epithelial cells and limiting the loss of stromal CAV1 are crucial for improving the response to radiotherapy. Additionally, targeting activated stromal fibroblasts by limiting fibroblast activation plays a key role in inhibiting the resistance-promoting CAV1-dependent signals of the tumor stroma.
FRONTIERS IN ONCOLOGY
(2022)
Correction
Cell Biology
Violetta Ritter, Franziska Krautter, Diana Klein, Verena Jendrossek, Justine Rudner
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Luca Hegedues, Kata D. Szuecs, Matthias Kudla, Julian Heidenreich, Verena Jendrossek, Samuel Pena-Llopis, Tamas Garay, Andras Czirok, Clemens Aigner, Till Ploenes, Silvia Vega-Rubin-de-Celis, Balazs Hegedues
Summary: This study investigated the effect of Nintedanib on SRC kinase, one of its targets, in MPM cells. The results showed that Nintedanib did not inhibit SRC activation and even increased SRC phosphorylation in some MPM cell lines. However, combination treatment with the SRC inhibitor Dasatinib reversed this effect. The study also revealed that combination treatment promoted autophagy in MPM cells, which was independent of the AKT/mTOR and ERK pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Kexu Xiang, Christian Kalthoff, Corinna Muench, Verena Jendrossek, Johann Matschke
Summary: Inhibition of SLC25A1 leads to accumulation of oncometabolite D-2-HG, increasing the sensitivity of cancer cells to radiotherapy and delaying DNA damage repair. Combining SLC25A1 inhibition with PARP or DNA-PKcs inhibitors further enhances radiotherapy-induced DNA damage and delays repair, resulting in increased radiosensitivity of cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Sied Kebir, Vivien Ullrich, Pia Berger, Celia Dobersalske, Sarah Langer, Laurel Rauschenbach, Daniel Trageser, Andreas Till, Franziska K. Lorbeer, Anja Wieland, Timo Wilhelm-Buchstab, Ashar Ahmad, Holger Froehlich, Igor Cima, Shruthi Prasad, Johann Matschke, Verena Jendrossek, Marc Remke, Barbara M. Gruener, Alexander Roesch, Jens T. Siveke, Christel Herold-Mende, Tobias Blau, Kathy Keyvani, Frank K. H. van Landeghem, Torsten Pietsch, Jorg Felsberg, Guido Reifenberger, Michael Weller, Ulrich Sure, Oliver Bruestle, Matthias Simon, Martin Glas, Bjoern Scheffler
Summary: This study aimed to identify and target tumor cells that can survive, adapt, and expand under primary therapy in glioblastoma. The researchers found that ALDH1A1+/pAKT+ subclones accumulate and acquire drug resistance in response to temozolomide treatment. They propose a combination therapy of temozolomide and AKT inhibitors as a potential treatment strategy.
CLINICAL CANCER RESEARCH
(2023)
Article
Neurosciences
Maya Junghans, Felix John, Hilal Cihankaya, Daniel Schliebs, Konstanze F. Winklhofer, Verian Bader, Johann Matschke, Carsten Theiss, Veronika Matschke
Summary: This study found that the levels of reactive oxygen species (ROS) were elevated in motor neurons of an ALS model, leading to an increased number of DNA damage response proteins. The study also identified a deficit in ROS detoxification mechanisms in the ALS model. The results suggest that maintaining redox homeostasis may play a key role in ALS therapy.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Oncology
Sara Jennrich, Martin Pelzer, Tobias Tertel, Benjamin Koska, Melanie Vullings, Basant Kumar Thakur, Verena Jendrossek, Beate Timmermann, Bernd Giebel, Justine Rudner
Summary: Glioblastoma multiforme (GBM) is an aggressive tumor of the central nervous system with limited treatment options. Radiation therapy, specifically proton-based irradiation, shows promising results in treating GBM tumors. Extracellular vesicles (EVs) released by GBM cells can potentially serve as markers for predicting tumor response to radiotherapy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Christina Wenzek, Philine Steinbach, Florian Wirsdoerfer, Kathrin Sutter, Julia D. Boehme, Robert Geffers, Robert Klopfleisch, Dunja Bruder, Verena Jendrossek, Jan Buer, Astrid M. Westendorf, Torben Knuschke
Summary: CD47 plays a significant role in immune responses, but its role in antiviral immunity is not fully understood. This study revealed that CD47-deficient mice showed enhanced clearance of IAV, and this enhanced clearance was mediated by aM Phi. The study also found that CD47 restricts HB expression in aM Phi after IAV and SARS-CoV-2 infection, and HB enhances the IFN-b response, showing antiviral properties.
Article
Biochemistry & Molecular Biology
Maurice Dellin, Ina Rohrbeck, Purva Asrani, Julian A. Schreiber, Nadine Ritter, Frank Glorius, Bernhard Wuensch, Thomas Budde, Louisa Temme, Timo Struenker, Birgit Stallmeyer, Frank Tuettelmann, Sven G. Meuth, Marc Spehr, Johann Matschke, Andrea Steinbicker, Christos Gatsogiannis, Raphael Stoll, Nathalie Strutz-Seebohm, Guiscard Seebohm
Summary: The study identified two binding sites, PS1 and S0, for PI(3,5)P-2 on the KCNQ1/KCNE1 channel, and found that binding of PI(3,5)P-2 to both sites is necessary for stabilizing the channel's open state.
BIOLOGICAL CHEMISTRY
(2023)
Article
Oncology
Nika Guberina, Florian Wirsdoerfer, Martin Stuschke, Verena Jendrossek
Summary: The risk of overlapping pulmonary toxicity induced by thoracic radio(chemo)therapy and immune checkpoint inhibitor therapy in the treatment of patients suffering from non-small cell lung cancer (NSCLC) is a significant challenge in successful radioimmunotherapy. Factors such as patient selection, monitoring biomarkers, and early detection of lung tissue changes play crucial roles in optimizing treatment outcomes and minimizing side effects. The concurrent use of radiotherapy and immunotherapy can provide new curative options for locally advanced NSCLC patients, but careful management of adverse events, especially in the lung, is necessary.
Article
Immunology
Kristina Zec, Stephanie Thiebes, Jenny Bottek, Devon Siemes, Philippa Spangenberg, Duc Viet Trieu, Nils Kirstein, Nirojah Subramaniam, Robin Christ, Diana Klein, Verena Jendrossek, Maria Loose, Florian Wagenlehner, Jadwiga Jablonska, Thilo Bracht, Barbara Sitek, Bettina Budeus, Ludger Klein-Hitpass, Dirk Theegarten, Olga Shevchuk, Daniel R. Engel
Summary: This study provides novel insights into the molecular adaptations of alveolar macrophages (AM) upon lung infection with Streptococcus pneumoniae. The results reveal a strong neutrophil-associated proteomic signature in AM, and identify CD11b as a central molecular hub influencing neutrophil recruitment, activation, and migration.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Yoshiyuki Henning, Katrin Willbrand, Safa Larafa, Gesa Weissenberg, Veronika Matschke, Carsten Theiss, Gina-Eva Goertz, Johann Matschke
Summary: Age-related macular degeneration (AMD) is the most common blinding disease in elderly. Impaired energy metabolism in retinal pigment epithelium (RPE) cells and dysregulated hypoxia-inducible factors (HIFs) play important roles in the pathophysiology of AMD. Smoking, a modifiable risk factor for AMD, is known to impair mitochondrial integrity. In this study, a cell-based assay was established to investigate the effects of smoking on mitochondrial function and HIF signaling in RPE cells under different oxygen levels. The results showed that smoking impaired mitochondrial integrity and glycolytic rate, and the effects were HIF-dependent. This in vitro model provides insights into the regulation of glycolysis under normal oxygen conditions, which is important for understanding RPE metabolism in health and disease.
CELL DEATH DISCOVERY
(2023)
Meeting Abstract
Oncology
Razan Hessenow, Carina Behrends, Xixi Lin, Emil Mladenov, Johann Matschke, Aashish Soni, Martin Stuschke, George Iliakis, Verena Jendrossek, Beate Timmermann
STRAHLENTHERAPIE UND ONKOLOGIE
(2023)
Meeting Abstract
Immunology
C. Wenzek, P. Steinbach, F. Wirsdoerfer, K. Sutter, J. D. Boehme, R. Geffers, D. Bruder, V. Jendrossek, J. Buer, A. M. Westendorf, T. Knuschke
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)