Review
Medicine, Research & Experimental
Charlotte Wallaeys, Natalia Garcia-Gonzalez, Claude Libert
Summary: Paneth cells, located in the crypts of Lieberkuhn of the small intestine, play diverse roles in maintaining intestinal health by providing niche factors, regulating microbiome composition, performing phagocytosis and efferocytosis, taking up heavy metals, and preserving barrier integrity. Dysfunctions in these functions can lead to inflammatory and infectious diseases. This review discusses the functions, mechanisms, consequences of Paneth cell dysfunction, and available tools for studying Paneth cells.
EMBO MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Connie Fung, Lisa M. Fraser, Gabriel M. Barron, Matthew B. Gologorsky, Samantha N. Atkinson, Elias R. Gerrick, Michael Hayward, Jennifer Ziegelbauer, Jessica A. Li, Katherine F. Nico, Miles D. W. Tyner, Leila B. DeSchepper, Amy Pan, Nita H. Salzman, Michael R. Howitt
Summary: Succinate produced by commensal protist T. mu stimulates tuft cells, leading to type 2 immunity. Microbial-derived succinate increases Paneth cell numbers and alters the AMP landscape in the small intestine. Tuft cells utilize SUCNR1 and succinate sensing to modulate bacterial homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Environmental Sciences
Chenbin Cui, Xinru Wang, Yao Zheng, Lindeng Li, Fangke Wang, Hongkui Wei, Jian Peng
Summary: A study found that treatment with deoxynivalenol (DON) reduced the number of Paneth cells in mouse intestines. However, disrupting Paneth cells using dithizone worsened intestinal injury and affected intestinal stem cells and microbiota in DON-treated mice. Surprisingly, the number of goblet cells and proliferative cells increased after treatment with dithizone and DON.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Multidisciplinary Sciences
Nathalie Coutry, Julie Nguyen, Salima Soualhi, Francois Gerbe, Victoria Meslier, Valerie Dardalhon, Mathieu Almeida, Benoit Quinquis, Florence Thirion, Fabien Herbert, Imene Gasmi, Ali Lamrani, Alicia Giordano, Pierre Cesses, Laure Garnier, Steeve Thirard, Denis Greuet, Chantal Cazevieille, Florence Bernex, Christelle Bressuire, Douglas Winton, Ichiro Matsumoto, Herve M. Naomi And, Naomi Taylor, Philippe Jay
Summary: Imbalance of gut microbiota is associated with pathological conditions. We propose a three-step mechanism for dysbiosis initiation, involving Paneth cell dysfunction, activation of epithelial tuft cells, and chronic inflammation. Paneth cells play an essential role in maintaining a balanced microbiota and preventing dysbiosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Agriculture, Dairy & Animal Science
Malgorzata Gierynska, Lidia Szulc-Dabrowska, Justyna Struzik, Matylda Barbara Mielcarska, Karolina Paulina Gregorczyk-Zboroch
Summary: This article discusses the importance of the gastrointestinal tract and the key mechanisms of communication between the gut microbiota and epithelial cells in maintaining intestinal homeostasis. The article emphasizes the role of the mucosal barrier and how intestinal epithelial cells maintain barrier integrity through mucus secretion, formation of intercellular junctions, and production of antimicrobial peptides. The article also discusses how imbalances in regulation can lead to barrier damage and dysbiosis, contributing to inflammatory and metabolic diseases.
Article
Biochemistry & Molecular Biology
Jolanta Artym, Michal Zimecki
Summary: Women's intimate health is influenced by factors like metabolic disorders, hormonal balance, and the microbiota of the genital tract. Maintaining a balance of symbiotic bacteria like Lactobacillus is crucial for preventing infections and inflammation in the genital tract. The protein lactoferrin plays a key role in bridging innate and acquired immunity and has prebiotic activity that can benefit the microbiota in the gastrointestinal and genital tract.
Article
Biochemistry & Molecular Biology
Bin Wang, Xuheng Chen, Zhiyuan Chen, Huiwen Xiao, Jiali Dong, Yuan Li, Xiaozhou Zeng, Jinjian Liu, Guoyun Wan, Saijun Fan, Ming Cui
Summary: In this study, the researchers found that stable colonization of live A. muciniphila at lesion sites is essential for its anti-inflammatory function. They also developed a modified A. muciniphila that had enhanced inflammation targeting and intestinal colonization under an external magnetic field, leading to improved therapeutic efficacy against intestinal diseases. The findings further revealed that this modified A. muciniphila could drive M2 macrophage polarization by upregulating the protein level of IL-4 at inflammatory loci. These results demonstrate the importance of A. muciniphila in treating intestinal diseases.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Patrizia Malfa, Laura Brambilla, Silvana Giardina, Martina Masciarelli, Diletta Francesca Squarzanti, Federica Carlomagno, Marisa Meloni
Summary: The aim of this study was to evaluate the efficacy of a multi-strain probiotic formulation in inhibiting urogenital pathogen adhesion and growth. The results showed that the multi-strain formulation completely inhibited Trichomonas vaginalis infection and reduced Candida glabrata and Neisseria gonorrheae growth. Each single strain also exhibited significant antimicrobial activity against Escherichia coli. The study demonstrated that the three strains and their mixture are effective in preventing urogenital infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yi Hong, Zhou Zhou, Lingzi Yu, Keyang Jiang, Jiamiao Xia, Yuling Mi, Caiqiao Zhang, Jian Li
Summary: The feeding of L. salivarius + L. agilis in hens can improve egg-laying rate, egg weight, and albumen's amino acid levels, as well as enhance intestinal microflora and metabolism profile.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Wenzhen Yuan, Xingpeng Xiao, Xuan Yu, Fuquan Xie, Pengya Feng, Kamran Malik, Jingyuan Wu, Ze Ye, Peng Zhang, Xiangkai Li
Summary: The study showed that oxaliplatin treatment can alter the gut microbiota, and probiotics can help alleviate the damage caused by oxaliplatin to the intestines.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Review
Medicine, General & Internal
Barbara Skrzydlo-Radomanska, Bozena Cukrowska
Summary: Small Intestinal Bacterial Overgrowth (SIBO) is a form of dysbiosis that involves excessive bacterial colonization in the small intestine, with some bacteria more typical of the colon microbiota. The prevalence of SIBO has been estimated to range from 2.5% to 22%, increasing with age and comorbidities. The availability of noninvasive breath tests has led to an increase in diagnosed SIBO cases. This review provides an overview of the pathophysiology, manifestations, diagnostics, and recommended management of SIBO.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
J. I. Ramirez-Perez, M. Abud-Archila, S. L. Ovando-Chacon, R. E. Soria-Guerra, M. A. Ruiz-Cabrera, C. I. Godinez-Hernandez, A. Grajales-Lagunes
Summary: A completely randomized experimental design was conducted to evaluate the effect of coadministration of Lpb. fabifermentans and inulin/agave fructans mixtures on gut microbiota. The results showed that the combination of fructan mixtures and Lpb. fabifermentans significantly decreased glucose, cholesterol, and triglycerides levels, and improved short-chain fatty acids concentration throughout the gastrointestinal tract. It also promoted an increase in the abundance of beneficial intestinal microbiota. Therefore, the inclusion of fructan mixtures in combination with Lpb. fabifermentans could be a good alternative for the development of functional foods that enhance consumer health.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Maria Naama, Shahar Telpaz, Aya Awad, Shira Ben-Simon, Sarina Harshuk-Shabso, Sonia Modilevsky, Elad Rubin, Jasmin Sawaed, Lilach Zelik, Mor Zigdon, Nofar Asulin, Sondra Turjeman, Michal Werbner, Supapit Wongkuna, Rachel Feeney, Bjoern O. Schroeder, Abraham Nyska, Meital Nuriel-Ohayon, Shai Bel
Summary: Colonic goblet cells secrete mucus to protect against bacterial invasion and inflammation. Activation of autophagy in mice via Beclin 1 reduces endoplasmic reticulum (ER) stress, leading to thicker and less penetrable mucus production. This ER-stress-mediated regulation of mucus secretion is dependent on the microbiota and the Nod2 gene, and it plays a critical role in maintaining intestinal homeostasis.
CELL HOST & MICROBE
(2023)
Article
Agriculture, Dairy & Animal Science
Lingzi Yu, Xiaochen Xie, Keyang Jiang, Yi Hong, Zhou Zhou, Yuling Mi, Caiqiao Zhang, Jian Li
Summary: The study found that in the early stage of intestinal inflammation in chickens, Paneth cells were impaired but quickly recovered, leading to a decrease in intestinal stem cell activity initially, followed by rapid recovery.
Article
Environmental Sciences
Shuqiu Yang, Zhonghua Xiong, Tianfang Xu, Chengcheng Peng, Aiming Hu, Wenjuan Jiang, Zhiwei Xiong, Yunhui Wu, Fan Yang, Huabin Cao
Summary: This study investigated the effects of compound probiotics on intestinal dysfunction and cecal microbiota dysregulation induced by cadmium in broilers. The results showed that cadmium caused intestinal villus damage and inflammation, down-regulated genes related to intestinal barrier and inflammatory factors, and up-regulated certain other genes. However, the addition of compound probiotics effectively improved these changes. Furthermore, the analysis of intestinal microbiota showed that supplementation with compound probiotics increased the abundance of beneficial bacteria and regulated pathways related to metabolism and translation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Infectious Diseases
Augusto E. Bivona, Andres Sanchez Alberti, Marina N. Matos, Natacha Cerny, Alejandro C. Cardoso, Celina Morales, German Gonzalez, Silvia I. Cazorla, Emilio L. Malchiodi
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Biochemistry & Molecular Biology
Valeria P. Sulsen, Emilio F. Lizarraga, Orlando G. Elso, Natacha Cerny, Andres Sanchez Alberti, Augusto E. Bivona, Emilio L. Malchiodi, Silvia I. Cazorla, Cesar A. N. Catalan
Article
Microbiology
A. E. Bivona, L. Czentner, A. Sanchez Alberti, N. Cerny, A. C. Cardoso Landaburu, C. Nevot, O. Estevez, J. D. Marco, M. A. Basombrio, E. L. Malchiodi, S. Cazorla
JOURNAL OF CLINICAL MICROBIOLOGY
(2019)
Article
Immunology
Andres Sanchez Alberti, Augusto E. Bivona, Marina N. Matos, Natacha Cerny, Kai Schulze, Sebastian Weissmann, Thomas Ebensen, German Gonzalez, Celina Morales, Alejandro C. Cardoso, Silvia Cazorla, Carlos A. Guzman, Emilio L. Malchiodi
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Orlando G. Elso, Augusto E. Bivona, Andres Sanchez Alberti, Natacha Cerny, Lucas Fabian, Celina Morales, Cesar A. N. Catalan, Emilio L. Malchiodi, Silvia Cazorla, Valeria P. Sulsen
Article
Immunology
Natacha Cerny, Augusto Ernesto Bivona, Andres Sanchez Alberti, Sebastian Nicolas Trinitario, Celina Morales, Alejandro Cardoso Landaburu, Silvia Ines Cazorla, Emilio Luis Malchiodi
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Maria Jose Martinez Monteros, Carolina Maldonado Galdeano, Maria Florencia Balcells, Ricardo Weill, Juan Andres De Paula, Gabriela Perdigon, Silvia Ines Cazorla
Summary: Probiotic supplementation can reduce symptoms of NSAID-induced inflammation in the small intestine, increase antimicrobial activity in the intestines, and promote changes in the gut microbiota to maintain intestinal homeostasis.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
B. Layus, M. A. Gomez, S. Cazorla, A. Rodriguez
Summary: The study showed that the cell-free supernatant of Lactiplantibacillus plantarum CRL759 can reduce inflammatory parameters and clinical signs of ocular inflammation, both in vitro and in vivo. This promising strategy may provide a safe and effective alternative for those refractory to conventional therapies for ocular inflammation.
BENEFICIAL MICROBES
(2021)
Article
Immunology
Jose Maria Lemme-Dumit, Silvia Ines Cazorla, Gabriela Del Valle Perdigon, Carolina Maldonado-Galdeano
Summary: The study shows that oral administration of probiotics or their sub-cellular fraction can reduce bacterial burden in the spleen and liver after Salmonella challenge. The beneficial effects of probiotic-derived CW after challenge are attributed to Th1-type cell-mediated immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Nutrition & Dietetics
Florencia Balcells, Maria Jose Martinez Monteros, Alba Lorena Gomez, Silvia Ines Cazorla, Gabriela Perdigon, Carolina Maldonado-Galdeano
Summary: This study found that supplementation with Lactobacillus casei CRL 431 and/or its cell wall improved body weight, intestinal microbiota, and thymus structure and function in obese and aging mice, leading to a better immune system and quality of life.
Article
Medicine, General & Internal
Rodrigo Hernan Tomas-Grau, Diego Ploper, Cesar Luis avila, Esteban Vera Pingitore, Carolina Maldonado Galdeano, Silvina Chaves, Sergio Benjamin Socias, Agustin Stagnetto, Silvia Adriana Navarro, Rossana Elena Chahla, Monica Aguilar Lopez, Conrado Juan Llapur, Patricia Aznar, Maria Elena Alcorta, Dardo Costas, Isolina Flores, Dar Heinze, Gabriela Apfelbaum, Raul Mostoslavsky, Gustavo Mostoslavsky, Silvia Ines Cazorla, Gabriela del Valle Perdigon, Rosana Chehin
Summary: The COVID-19 pandemic has prompted the need for rapid development of diagnostic strategies. Research has shown that a proportion of individuals infected with COVID-19 develop antibodies, but only a small percentage have sufficient antibody levels to be considered as plasma donors.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Physical
Eliana Rita Solorzano, Paolo Pastore, Alessandro Dolmella, Silvia Cazorla, Maria Lucrecia Arias Cassara, Sankaran Venkatachalam Sankaran, Subbiah Thamotharan, Diego M. Gil
Summary: A detailed experimental and theoretical investigation was conducted on a chalcone derivative, DHMC, isolated from Argentinean type-Zuccagnia propolis. The compound was characterized using various spectroscopic techniques and its crystal structure was determined by X-ray diffraction. Theoretical investigations showed good agreement with experimental results in terms of molecular geometry and vibrational frequencies. The compound exhibited anti-leishmanial activity and molecular docking simulations predicted its binding potential to six different leishmanial protein targets.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Cell Biology
Rodrigo Hernan Tomas-Grau, Carolina Maldonado-Galdeano, Monica Aguilar Lopez, Esteban Vera Pingitore, Patricia Aznar, Maria Elena Alcorta, Eva Maria del Mar Velez, Agustin Stagnetto, Silvana Estefania Soliz-Santander, Cesar Luis Avila, Sergio Benjamin Socias, Dardo Costas, Rossana Elena Chahla, Gabriela Perdigon, Rosana Nieves Chehin, Diego Ploper, Silvia Ines Cazorla
Summary: The early development of vaccines against SARS-CoV-2 has been successful, but age-related immunosenescence remains a challenge. Analysis of the humoral immune response in elderly volunteers who received Gam-COVID-Vac vaccine revealed that at least two doses were necessary to elicit a robust immune response, especially in individuals without previous infection. Antibody titers reached their highest levels at 28 days post-vaccination and remained stable at 60 days, but started to decline at 180 days. Gender did not significantly affect the specific humoral immune responses at early time points, but at 60 days post-vaccination, females had more persistent antibody titers.
Article
Pharmacology & Pharmacy
Mariana G. Selener, Orlando Elso, Carla Grosso, Jimena Borgo, Maria Clavin, Emilio L. Malchiodi, Silvia Cazorla, Flavia Redko, Valeria P. Sulsen
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2019)
Review
Endocrinology & Metabolism
Carolina Maldonado Galdeano, Silvia Ines Cazorla, Jose Maria Lemme Dumit, Eva Velez, Gabriela Perdigon
ANNALS OF NUTRITION AND METABOLISM
(2019)