Article
Biochemistry & Molecular Biology
Aleena L. Patel, Lili Zhang, Shannon E. Keenan, Christine A. Rushlow, Cecile Fradin, Stanislav Y. Shvartsman
Summary: This study investigates the dynamics of transcriptional repression by Capicua (Cic) in early Drosophila embryos, revealing the speed limit and mechanism of rapid gene repression by Cic. It sets quantitative constraints on potential mechanisms for gene regulation by Cic, providing insights for future studies in gene regulation mechanisms.
Article
Biochemistry & Molecular Biology
Xin-Xin Kang, Qin-Qing Wang, Zhe Chi, Guang-Lei Liu, Zhong Hu, Zhen-Ming Chi
Summary: The study showed that by removing GATA-type transcriptional activator and repressor genes, as well as overexpressing mutated genes with specific residues, the pullulan production of Aureobasidium melanogenum P16 could be significantly increased.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Iti Saraav, Luisa Cervantes-Barragan, Philipp Olias, Yong Fu, Qiuling Wang, Leran Wang, Yi Wang, Matthias Mack, Megan T. Baldridge, Thaddeus Stappenbeck, Marco Colonna, L. David Sibley
Summary: Oral infection with Toxoplasma gondii exacerbates damage to the colon caused by chemical irritant and impairs wound healing by suppressing stem cell regeneration. Enhanced tissue damage is due to inflammatory monocytes releasing inflammatory mediators.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Abhishek Sinha, Vinson B. Fan, Aravinda-Bharathi Ramakrishnan, Nicole Engelhardt, Jennifer Kennell, Ken M. Cadigan
Summary: SOX9 promotes turnover of β-catenin independently of the destruction complex and proteasome in mammalian cell culture. This requires the activation of MAML2, which acts as a coactivator for SOX9 in antagonizing the Wnt/β-catenin pathway. This newly identified pathway provides a parallel mechanism for restricting Wnt/β-catenin signaling.
Article
Plant Sciences
Lu-Shen Li, Jun Ying, En Li, Ting Ma, Min Li, Li-Min Gong, Guo Wei, Yan Zhang, Sha Li
Summary: Plants have a dual defense system involving membrane receptors and disease-resistance proteins to combat microbial pathogens. CBP60b acts as a transcriptional activator of immunity genes and plays a role in both pathogen-induced defense and basal defense, being redundant with CBP60g and SARD1.
Article
Biochemistry & Molecular Biology
James K. Nunez, Jin Chen, Greg C. Pommier, J. Zachery Cogan, Joseph M. Replogle, Carmen Adriaens, Gokul N. Ramadoss, Quanming Shi, King L. Hung, Avi J. Samelson, Angela N. Pogson, James Y. S. Kim, Amanda Chung, Manuel D. Leonetti, Howard Y. Chang, Martin Kampmann, Bradley E. Bernstein, Volker Hovestadt, Luke A. Gilbert, Jonathan S. Weissman
Summary: CRISPRoff is a programmable epigenetic memory writer that can heritably alter gene expression by initiating specific DNA methylation and gene repression. The tool utilizes a single dead Cas9 fusion protein to establish these modifications, which are maintained through cell division and differentiation processes. By pairing CRISPRoff with genome-wide screens and analysis of chromatin marks, rules for heritable gene silencing can be established, with the tool capable of targeting a wide range of genes beyond canonical CpG islands.
Article
Infectious Diseases
Thais Cabral Monica, Fernanda Evers, Beatriz Souza Lima Nino, Fernanda Pinto-Ferreira, Jose Wander Bregano, Mariana Ragassi Urbano, Guita Rubinsky-Elefant, Roberta Lemos Freire, Italmar Teodorico Navarro, Regina Mitsuka-Bregano
Summary: The study examined the seroprevalence and risk factors of toxoplasmosis and toxocariasis in schoolchildren in Jataizinho, Parana, revealing associations with factors such as the mother's education level, age, presence of cats in the household, income, and presence of dogs. There was also an association between the two zoonoses, highlighting the importance of implementing control measures to reduce the incidence and risks of sequelae in children.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Aleksandra Anna Bonini, Stefano Maggi, Giulia Mori, Dario Carnuccio, Danila Delfino, Davide Cavazzini, Alberto Ferrari, Alessia Levante, Yoshihiro Yamaguchi, Claudio Rivetti, Claudia Folli
Summary: This study investigated the functional characteristics of DinJ-YafQ system in Lacticaseibacillus paracasei. The results showed that both YafQ orthologs can digest ribosomal RNA with different efficiencies, and their activity is neutralized by DinJ. Moreover, DinJ and DinJ-YafQ can cooperatively bind to the promoter of the TA operon, preventing RNA polymerase from binding. It was also found that a single amino acid substitution in DinJ abolishes its DNA binding ability.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Jian Wang, Xiao-fen Liu, Hui-qin Zhang, Andrew C. Allan, Wen-qiu Wang, Xue-ren Yin
Summary: The study demonstrates that exogenous ASA treatment significantly reduces ethylene production and inhibits the activity of related enzymes in kiwifruit tissues. Transcriptome analysis and transcription factor screening reveal ASA-responsive regulators for ethylene biosynthetic genes. Additionally, abundantly expressed genes are found in both ASA-treated and control tissues. The findings suggest that ASA inhibits ethylene biosynthesis by regulating protein activity at a post-transcriptional level.
HORTICULTURE RESEARCH
(2022)
Article
Microbiology
Asmaa M. El-kady, Wafa Abdullah Al-Megrin, Iman A. M. Abdel-Rahman, Eman Sayed, Eman Abdullah Alshehri, Majed H. Wakid, Fadi M. Baakdah, Khalil Mohamed, Hayam Elshazly, Hussah M. Alobaid, Safa H. Qahl, Hatem A. Elshabrawy, Salwa S. Younis
Summary: Ginger extract demonstrated significant therapeutic effects in mice with chronic toxoplasmosis, reducing inflammation, cyst count, and neuronal injury. It can be considered as a safe and effective treatment for chronic toxoplasmosis.
Article
Medicine, Research & Experimental
Lei Bao, Ashwani Kumar, Ming Zhu, Yan Peng, Chao Xing, Jennifer E. Wang, Yingfei Wang, Weibo Luo
Summary: SAP30 is an important protein in breast cancer, and its upregulation is associated with unfavorable prognosis. Research has shown that SAP30 promotes tumor growth and metastasis through mechanisms other than canonical gene silencing. Additionally, SAP30 enhances cell motility, angiogenesis, and lymphangiogenesis by interacting with other proteins, thereby driving breast cancer progression.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Qiang Zhang, Shuangshuang Wang, Qiujin Xie, Yuanjun Xia, Lei Lu, Mingxing Wang, Gang Wang, Siyu Long, Yunfei Cai, Ling Xu, Ertao Wang, Yina Jiang
Summary: Terrestrial plants establish symbiotic relationships with arbuscular mycorrhizal fungi through dynamic control of nutrient exchange. The ERM1/WRI5a-ERF12-TOPLESS negative feedback loop plays a key role in maintaining this mutualistic symbiosis.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Nisha Misra, Manohar Damara, Pierre Chambon
Summary: This article investigates the role of transcriptional regulators RevErbα, E4BP4, and CRY1/PER1 in generating circadian rhythms. Molecular and genetic analyses reveal that the chromatin protein HP1α plays a crucial role in transcriptional repressions of circadian clock genes and their output genes. The presence of HP1α is critical for chromatin compaction, formation of repressed biomolecular hydrophobic condensates, and recruitment and methylation of DNA methylating enzymes on the intronic deoxyCpG islands within the circadian output genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Y. Lobanova, G. Filonova, D. Kaplun, N. Zhigalova, E. Prokhortchouk, S. Zhenilo
Summary: TRIM28 enhances SUMOylation of Kaiso, leading to a decreased methyl-dependent repression ability. Additionally, Kaiso enhances SUMOylation of TRIM28, affecting the transcriptional activity of Kaiso.
Article
Biochemistry & Molecular Biology
Andrew L. Spencley, Shiran Bar, Tomek Swigut, Ryan A. Flynn, Cameron H. Lee, Liang-Fu Chen, Michael C. Bassik, Joanna Wysocka
Summary: The HUSH complex can recognize and silence foreign DNA without prior exposure. Endogenous targets of the complex fall into two classes based on the presence or absence of H3K9me3, which are distinguished by their transposon content and response to loss of HUSH. A genetic rearrangement induces a switch in HUSH targeting at the Sox2 locus, leading to silencing. HUSH interacts with WDR82 and MPP8 to associate with nascent RNA at sites of high RNAPII occupancy. The complex exploits evolutionarily ancient transcription termination machinery for co-transcriptional chromatin targeting and genome surveillance.
Article
Immunology
Edward A. Vizcarra, Arzu Ulu, Tyler A. Landrith, Xinru Qiu, Adam Godzik, Emma H. Wilson
Summary: A pro-inflammatory response is important for preventing clinical disease caused by Toxoplasma gondii reaction within the brain. T cells play a crucial role in preventing such disease, and the presence of specific signals, such as glutamate, can promote T cell function within the brain, contributing to protective immunity against CNS-infiltrating pathogens and neuroinflammation.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Immunology
Kristina V. Bergersen, Ashley D. Ramirez, Bill Kavvathas, Frances Mercer, Emma H. Wilson
Summary: This study identifies a novel response of human neutrophil-like cells to Toxoplasma cysts and may indicate a role for neutrophils in the clearance of cysts during human infection with Toxoplasma.
PARASITE IMMUNOLOGY
(2023)
Article
Microbiology
Edward A. Vizcarra, Amber L. Goerner, Arzu Ulu, David D. Hong, Kristina V. Bergersen, Michael A. Talavera, Karine Le Roch, Emma H. Wilson, Michael W. White
Summary: The recrudescence of Toxoplasma cysts is the cause of clinical disease in immunocompromised individuals. A new ex vivo model revealed the cell biology and gene expression underlying the reactivation of bradyzoite-cyst, and discovered previously unknown complexities in the clinically important bradyzoite stage. This revised model opens up opportunities for further study of these novel developmental features of the Toxoplasma intermediate life cycle.