Article
Immunology
Kristina Zec, Stephanie Thiebes, Jenny Bottek, Devon Siemes, Philippa Spangenberg, Duc Viet Trieu, Nils Kirstein, Nirojah Subramaniam, Robin Christ, Diana Klein, Verena Jendrossek, Maria Loose, Florian Wagenlehner, Jadwiga Jablonska, Thilo Bracht, Barbara Sitek, Bettina Budeus, Ludger Klein-Hitpass, Dirk Theegarten, Olga Shevchuk, Daniel R. Engel
Summary: This study provides novel insights into the molecular adaptations of alveolar macrophages (AM) upon lung infection with Streptococcus pneumoniae. The results reveal a strong neutrophil-associated proteomic signature in AM, and identify CD11b as a central molecular hub influencing neutrophil recruitment, activation, and migration.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Plant Sciences
Regina Ninoles, Dolores Planes, Paloma Arjona, Carmen Ruiz-Pastor, Ruben Chazarra, Joan Renard, Eduardo Bueso, Javier Forment, Ramon Serrano, Ilse Kranner, Thomas Roach, Jose Gadea
Summary: Understanding the genetic factors involved in seed longevity is crucial in agriculture and ecology. This study investigated the mechanisms behind differing seed longevity in Arabidopsis thaliana, revealing the importance of redox poise and stored mRNA in determining seed viability. Multiple factors contribute to seed longevity, including heat-shock factors and RNA regulation.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Food Science & Technology
Jianfeng Huang, Xinxin Su, Qiyan Jia, Haoran Chen, Shaoxiao Zeng, Hui Xu
Summary: This study investigated the potential mechanism of action of tea polyphenols (TPs) to enhance heat resistance in Drosophila and the attenuating effect of heat treatment on their efficacy. The results showed that TPs were able to prolong the survival time of Drosophila under high-temperature stress, but this effect was significantly reduced with increasing heat-treatment time. The composition of TPs changed after heat treatment. Transcriptomic and metabolomic analysis revealed the pathways and processes involved in the heat tolerance improvement by TPs. The study provides important insights for the development and utilization of tea's heat-protection function.
Article
Plant Sciences
Peisen Su, Chao Sui, Yufei Niu, Jingyu Li, Shuhan Wang, Fanting Sun, Jun Yan, Shangjing Guo
Summary: Drought is a significant stress that reduces crop yields and quality globally. This study evaluated the drought tolerance of 15 wheat cultivars and explored the underlying mechanisms. The findings revealed that antioxidant capacity is associated with higher drought tolerance in resistant wheat cultivars. Transcriptomic analysis demonstrated distinct mechanisms of drought tolerance between two wheat cultivars.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biology
Athina Georgiadou, Claire Dunican, Pablo Soro-Barrio, Hyun Jae Lee, Myrsini Kaforou, Aubrey J. Cunnington
Summary: Recent research has focused on improving the translation of animal models to human disease, but determining the relevance of animal models remains a challenge. In this study, comparative transcriptomics were used to evaluate the systemic host response in humans with different clinical manifestations of malaria and commonly used mouse models. The results showed that the Plasmodium yoelii 17XL infection model in mice closely reproduced the gene expression changes seen in severe malaria in humans. However, there was also significant discordance in gene expression changes between different host species and models.
Article
Genetics & Heredity
Lakbira Sheffield, Noah Sciambra, Alysa Evans, Eli Hagedorn, Casey Goltz, Megan Delfeld, Haley Kuhns, Janna L. Fierst, Stanislava Chtarbanova
Summary: Older Drosophila are more susceptible to RNA virus infection and succumb more quickly due to a defect in disease tolerance. Compared to younger flies, older Drosophila exhibit a more complex immune response to virus infection.
G3-GENES GENOMES GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Angela del Castillo-Izquierdo, Jose Maria Moreno-Navarrete, Jessica Latorre, Maria Arnoriaga-Rodriguez, Marta Ballanti, Giovanni Monteleone, Omero Alessandro Paoluzi, Geltrude Mingrone, Josep Puig, Rafael Ramos, Josep Garre-Olmo, Mariona Jove, Reinald Pamplona, Manuel Portero-Otin, Joaquim Sol, Philippe Lefebvre, Bart Staels, Massimo Federici, Jose Manuel Fernandez-Real, Jordi Mayneris-Perxachs
Summary: In this study, the potential role of DPP9 in the gastrointestinal tract was explored, and its association with oxidative stress, antiviral pathways, and metabolites was identified. Promising existing drugs targeting DPP9-associated genes were also identified for potential repositioning against viral infection.
Review
Microbiology
Marta Arch, Maria Vidal, Romina Koiffman, Solomon Tibebu Melkie, Pere-Joan Cardona
Summary: This article discusses the latest research advances in the study of the memory mechanisms of the innate immune response and introduces the use of the fruit fly Drosophila melanogaster as a model for studying these mechanisms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
John Verigos, Dimitris Kordias, Styliani Papadaki, Angeliki Magklara
Summary: Cancer stem cells play a significant role in chemoresistance, tumor recurrence, and metastasis in breast cancer, making them a promising target for new therapies. Through RNA-sequencing, researchers identified differential gene expression patterns between breast CSCs and non-CSCs, with the TRPM4 gene being found to be overexpressed in tumorspheres and affecting stemness properties. Inhibiting TRPM4 showed potential anti-tumor effects by targeting the bCSC subpopulation, suggesting a novel therapeutic approach for improving breast cancer treatments.
Article
Biochemistry & Molecular Biology
Qiong Zong, Bin Mao, Hua-Bao Zhang, Bing Wang, Wen-Juan Yu, Zhi-Wei Wang, Yu-Feng Wang
Summary: This study reveals the deubiquitylating effects induced by Wolbachia infection, suggesting that Wolbachia can widely deubiquitinate proteins that have crucial functions in male fertility of their hosts, but are not involved in cytoplasmic incompatibility (CI). The findings provide new insights into the regulatory mechanisms of endosymbiont/host interactions and male fertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Enliang Liu, Linli Xu, Zhengqian Luo, Zhiqiang Li, Guohui Zhou, Haifeng Gao, Furong Fang, Jun Tang, Yue Zhao, Zhilin Zhou, Ping Jin
Summary: Drought has negative impacts on plant growth, development, agriculture, and food production. This study investigated the mechanisms of drought tolerance in seven sweet potato cultivars. Different drought-tolerance mechanisms were identified, providing important information for the selection of sweet potatoes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Desalegn D. Serba, Xiaoxi Meng, James Schnable, Elfadil Bashir, J. P. Michaud, P. V. Vara Prasad, Ramasamy Perumal
Summary: The study used RNA-Seq technology to analyze the effects of sugarcane aphid on moderately resistant and susceptible genotypes, revealing transcriptome differences between the two genotypes at multiple time points after infestation by SCA. The resistant genotype exhibited differential expression of several gene clusters during SCA infestation, while the susceptible genotype showed a series of down-regulated genes on the basis of the resistant genotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Qiuyu Wang, Pengfei Wang, Pingping Liu, Jie Ou
Summary: The study found significant differences in the expression levels of 956 genes in biofilms compared with planktonic cells, suggesting that two-component regulatory system (TCS) and quorum sensing (QS) regulate V. parahaemolyticus biofilm formation by affecting important factors such as flagellar motility, Extracellular polymeric substance (EPS) secretion, tripartite ATP-independent (TRAP) transport system, and ATP binding cassette (ABC) transport system. This work in transcriptomics provides a basis for future studies examining the complex network systems that regulate bacterial biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Gongliang Liu, Xinyu Bi, Changli Tao, Yongtao Fei, Sujuan Gao, Jinglong Liang, Weidong Bai
Summary: This study elucidated the transcriptional changes in Z. mellis in response to high-glucose osmotic stress, confirming several stress response pathways closely related to high-glucose tolerance.
FOOD SCIENCE AND HUMAN WELLNESS
(2021)
Article
Biology
Noushin Hadadi, Martina Spiljar, Karin Steinbach, Melis colakoglu, Claire Chevalier, Gabriela Salinas, Doron Merkler, Mirko Trajkovski, Ana Domingos
Summary: Thermal adaptation is widely used to enhance or suppress thermogenic and mitochondrial activity in adipose tissues and has been suggested as a lifestyle intervention for weight maintenance. This study provides a systematic characterization of the tissue-specific transcriptional responses to temperature alterations in various organs of mice, highlighting the importance of integrative transcriptional analysis in understanding temperature-mediated biological programming.
Review
Developmental Biology
Katia Troha, Nicolas Buchon
WILEY INTERDISCIPLINARY REVIEWS-DEVELOPMENTAL BIOLOGY
(2019)
Article
Microbiology
Philip Houtz, Alessandro Bonfini, Xiaoli Bing, Nicolas Buchon
CELL HOST & MICROBE
(2019)
Article
Immunology
Katia Troha, Peter Nagy, Andrew Pivovar, Brian P. Lazzaro, Paul S. Hartley, Nicolas Buchon
Review
Immunology
Katia Troha, Janelle S. Ayres
TRENDS IN IMMUNOLOGY
(2020)
Review
Immunology
Justin L. McCarville, Grischa Y. Chen, Victor D. Cuevas, Katia Troha, Janelle S. Ayres
ANNUAL REVIEW OF IMMUNOLOGY, VOL 38
(2020)
Article
Agronomy
Xiao-Li Bing, Jessica Winkler, Joseph Gerlach, Gregory Loeb, Nicolas Buchon
Summary: The study found that multiple microbes isolated from field-collected Drosophila suzukii are pathogenic to both larvae and adults through different routes of infection, suggesting potential candidates for biocontrol of this species.
PEST MANAGEMENT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Melissa A. White, Alessandro Bonfini, Mariana F. Wolfner, Nicolas Buchon
Summary: After mating, Drosophila melanogaster females experience significant changes in their digestive system, triggered by the seminal fluid protein Sex Peptide (SP), to support egg production by altering gene expression and metabolism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Stephen P. Ellner, Nicolas Buchon, Tobias Dorr, Brian P. Lazzaro
Summary: The mutual negative feedbacks between pathogens and host immune response could lead to different infection outcomes even in similar hosts, potentially resulting in life-or-death differences. Further research on pathogen modifications may provide new, testable predictions regarding bimodal infection outcomes.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Biology
Alessandro Bonfini, Adam J. Dobson, David Duneau, Jonathan Reva, Xi Liu, Philip Houtz, Nicolas Buchon
Summary: The gut of Drosophila can adapt its size based on dietary changes, with nutrients promoting growth and sugar inhibiting it. The diet influences cell numbers, enterocyte size, stem cell proliferation, and translation, showing complex relationships between nutrition, epithelial dynamics, and cell size. Despite attempts to genetically rescue diet-induced effects on gut size, the impact of diet on enterocyte size can be enhanced when stem cell proliferation is deficient.
Review
Microbiology
Katia Troha, Janelle S. Ayres
Summary: During co-evolution with pathogens, hosts developed cooperative defense strategies to maintain their health. These strategies limit physiological damage and promote health without compromising the pathogen's survival and replication within the host. The study of cooperative defenses offers an opportunity to protect against global pandemics, especially in the absence of vaccines and rising antibiotic resistance.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Article
Virology
Jeffrey J. Hodgson, Nicolas Buchon, Gary W. Blissard
Summary: Little is known about the trafficking process of viral envelope proteins in insect cells. In this study, a stable Drosophila cell line was engineered to investigate the host proteins and cellular trafficking pathways involved in the delivery of the baculovirus envelope protein GP64 to the plasma membrane. Through a targeted RNA interference screen, several host genes were identified to be important for the trafficking of GP64 to the cell surface. The findings suggest that recycling plays a role in maintaining GP64 at the cell surface. This study provides insight into the understanding of how insect virus envelope proteins are transported and contributes to the knowledge of virus infection processes.
JOURNAL OF VIROLOGY
(2022)
Article
Cell Biology
Xi Liu, Peter Nagy, Alessandro Bonfini, Philip Houtz, Xiao-Li Bing, Xiaowei Yang, Nicolas Buchon
Summary: Gut microbes play an important role in the differentiation of gut stem cells, influencing epithelial composition and gut physiology. The balance between microbial pattern recognition pathways and damage response pathways is crucial for stem cell differentiation.
Article
Biology
Bretta Hixson, Xiao-Li Bing, Xiaowei Yang, Alessandro Bonfini, Peter Nagy, Nicolas Buchon
Summary: With the help of Aegypti-Atlas, we have gained insights into mosquito biology, including the regionalization of gut function, blood feeding response, and immune defenses. We have also observed that digestive and immune specializations are conserved across different mosquito species.
Article
Behavioral Sciences
David Duneau, Nicolas Buchon
Summary: This study examined whether sick individuals are more likely to be preyed upon by predators. Using Drosophila melanogaster flies as manipulable prey, the researchers found that flies chronically infected with a bacterium were not selectively preyed upon by jumping spiders. However, flies with induced colon cancer had a higher incidence of predation. The results suggest that disease can have lethal consequences through predation, even in invertebrates.
Article
Gastroenterology & Hepatology
Ajeet P. Singh, Yu-Han Hung, Michael T. Shanahan, Matt Kanke, Alessandro Bonfini, Michael K. Dame, Mandy Biraud, Bailey C. E. Peck, Oyebola O. Oyesola, John M. Freund, Rebecca L. Cubitt, Ennessa G. Curry, Liara M. Gonzalez, Gavin A. Bewick, Elia D. Tait-Wojno, Natasza A. Kurpios, Shengli Ding, Jason R. Spence, Christopher M. Dekaney, Nicolas Buchon, Praveen Sethupathy
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2020)