Article
Cell Biology
I-Lun Hsin, Pei-Ju Wu, Sheau-Chung Tang, Chu-Chyn Ou, Hui-Yi Chang, Huang-Pin Shen, Jiunn-Liang Ko, Po-Hui Wang
Summary: ICG-001 inhibits endometrial cancer by reducing cell viability, cancer stem cell sphere formation, and cell cycle progression, while activating autophagy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Chemistry, Medicinal
Geoffroy Danieau, Sarah Morice, Sarah Renault, Regis Brion, Kevin Biteau, Jerome Amiaud, Marie Cade, Dominique Heymann, Frederic Lezot, Franck Verrecchia, Francoise Redini, Benedicte Brounais-Le Royer
Summary: This study analyzed the effects of ICG-001 on three human osteosarcoma cell lines and found that ICG-001 treatment significantly inhibited cell proliferation and induced cell cycle arrest, but unexpectedly increased cell migration. In a mouse model of osteosarcoma, ICG-001 did not affect tumor growth but significantly increased metastatic dissemination to the lungs.
Article
Endocrinology & Metabolism
Linlin Zhang, Kecheng Zhu, Jingzun Xu, Xiaojing Chen, Chunxiang Sheng, Deng Zhang, Yuying Yang, Lihao Sun, Hongyan Zhao, Xiao Wang, Bei Tao, Libin Zhou, Jianmin Liu
Summary: CBP and p300, as important histone acetyltransferases, have been found to affect bone density and strength. The study further demonstrates that CBP/p300 HAT regulates osteogenic gene expression by modulating transcription of beta-catenin and Stat1. These findings reveal an epigenetic mechanism responsible for maintaining bone homeostasis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Urology & Nephrology
Mingsheng Zhu, Xian Ling, Shan Zhou, Ping Meng, Qiyan Chen, Shuangqin Chen, Kunyu Shen, Chao Xie, Yaozhong Kong, Maosheng Wang, Lili Zhou
Summary: This study compared the therapeutic effects of two beta-catenin inhibitors, KYA1797K and ICG-001, on protecting against kidney aging. The results showed that KYA1797K had superior effects in preserving mitochondrial homeostasis, inhibiting cellular senescence, and retarding age-related kidney fibrosis. In addition, KYA1797K demonstrated better effects in inhibiting cellular senescence, suppressing mitochondrial dysfunction, and ameliorating fibrotic changes compared to ICG-001.
Article
Oncology
Cuicui Wu
Summary: The study investigated the synergistic effect of beta-catenin inhibitors, ICG-001 and pyrvinium (PP), with BTZ in treating BTZ-resistant MM cells. Results showed that these inhibitors not only increased apoptosis but also sensitized BTZ-resistant MM cells to BTZ, indicating their potential therapeutic application in MM.
Article
Biochemistry & Molecular Biology
Chikashi Minemura, Shunichi Asai, Ayaka Koma, Ikuko Kase-Kato, Nozomi Tanaka, Naoko Kikkawa, Atsushi Kasamatsu, Hidetaka Yokoe, Toyoyuki Hanazawa, Katsuhiro Uzawa, Naohiko Seki
Summary: Our recent study focused on the passenger strand of pre-miR-30e, miR-30e-3p, which was found to be downregulated in cancer tissues. We identified 11 target genes of miR-30e-3p that were highly expressed in head and neck squamous cell carcinoma (HNSCC) patients, and these genes were associated with poor survival rates. Silencing the expression of one of the target genes, SERPINE1, suppressed the malignant phenotypes of HNSCC cells. This study contributes to our understanding of the molecular pathogenesis of HNSCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Gianina Demarchi, Sofia Perrone, Gaela Esper Romero, Cristian De Bonis, Juan Pablo Casasco, Gustavo Sevlever, Silvia Ines Berner, Carolina Cristina
Summary: This article presents a case of an aggressive cavernous sinus tumor and discusses its response to treatment and the role of relevant biomarkers.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Qiu-Chi Ran, Sheng-Rong Long, Yan Ye, Chen Xie, Zhuo-Lin XuXiao, Yu-Song Liu, Hong-Xia Pang, Diwas Sunchuri, Nai-Chia Teng, Zhu-Ling Guo
Summary: This study identified tumor microenvironment-related genes associated with poor prognosis in HNSCC, providing potential new targets and approaches for HNSCC treatment.
JOURNAL OF DENTAL SCIENCES
(2021)
Article
Genetics & Heredity
Chikashi Minemura, Shunichi Asai, Ayaka Koma, Naoko Kikkawa, Mayuko Kato, Atsushi Kasamatsu, Katsuhiro Uzawa, Toyoyuki Hanazawa, Naohiko Seki
Summary: Analysis of miRNA expression in head and neck squamous cell carcinoma (HNSCC) revealed downregulation of the miR-30 family, especially miR-30e-5p and miR-30c-1-3p, which are associated with shorter patient survival. Further investigation showed that miR-30e-5p suppresses HNSCC cell migration and controls multiple oncogenic genes, including FOXD1. Knockdown of FOXD1 inhibited malignant phenotypes of HNSCC cells. These findings provide potential prognostic markers and therapeutic targets for HNSCC.
Article
Materials Science, Multidisciplinary
Fan Sheng, Yinli Luo, Di Wu, Jiachen Yuan, Ge Zheng, Yuhao Zheng, Zhehu Jin
Summary: This study investigates the effects of ICG-001 on the proliferation, invasion, and apoptosis of keloid fibroblasts, as well as the excessive deposition of collagen. It also explores the inhibitory effect of ICG-001 on tumor growth in a keloid mouse model. The results show that ICG-001 is effective in inhibiting keloid fibroblast proliferation and promoting apoptosis.
MATERIALS & DESIGN
(2022)
Article
Medicine, Research & Experimental
Xia Cheng, Dandan Liu, Xinxin Ren, You Nie, Yibing Zhao, Ruyu Chen, Hongwei Wang
Summary: This study investigated the effect of beta-catenin on sepsis-induced inflammatory lung injury and the underlying mechanisms. The results showed that inhibition of beta-catenin expression and blockage of beta-catenin/CBP interactions effectively decreased mortality, alleviated lung injury, and reduced inflammatory cytokine levels in the sepsis model mice.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Article
Oncology
Qingchao Meng, Lei Li, Liping Wang
Summary: This study found that CBX8 is highly expressed in laryngeal squamous cell carcinoma (LSCC), and it induces the epithelial to mesenchymal transition (EMT) process by activating the Wnt/β-catenin signaling pathway, promoting LSCC cell proliferation and migration, and inhibiting apoptosis, resulting in poor prognosis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Weifeng Xu, Caiyun Nie, Huifang Lv, BeiBei Chen, Jianzheng Wang, Saiqi Wang, Jing Zhao, Yunduan He, Xiaobing Chen
Summary: Based on molecular subtypes, this study classified hepatocellular carcinoma and explored the relationship between Wnt signaling and cancer development. The high Wnt subtype was found to be associated with immune suppression, poor prognosis, and low response to immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Amelie Kasperek, Anthony Beguin, Olivia Bawa, Kevin De Azevedo, Bastien Job, Christophe Massard, Jean-Yves Scoazec, Thierry Heidmann, Odile Heidmann
Summary: Human endogenous retrovirus HEMO is specifically expressed in tumors and may be linked to activation of the Wnt/β-catenin signaling pathway. Tumors with different levels of HEMO expression are negatively associated with immune response signatures. These findings suggest that HEMO could be a promising tumor biomarker and therapeutic target.
MOLECULAR ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Waqas Azeem, Ragnhild Maukon Bakke, Benjamin Gabriel, Silke Appel, Anne Margrete Oyan, Karl-Henning Kalland
Summary: Research indicates that axitinib and nitazoxanide did not significantly inhibit beta-catenin signaling in monocyte-derived dendritic cells, but exhibited lower cytotoxicity. Axitinib stimulated various aspects of dendritic cell function, such as IL12-p70 secretion, and countered IL-10 secretion.
Article
Physiology
Elim Na, Eri Allen, Lillia A. Baird, Christine V. Odom, Filiz T. Korkmaz, Anukul T. Shenoy, Adeline M. Matschulat, Matthew R. Jones, Darrell N. Kotton, Joseph P. Mizgerd, Xaralabos Varelas, Katrina E. Traber, Lee J. Quinton
Summary: During bacterial pneumonia, alveolar epithelial cells play a critical role and LIF signaling in epithelial cells regulates the response to bacterial pneumonia. However, LIFR may have more prominent roles in other cell types, which requires further investigation.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
Anukul T. Shenoy, Carolina Lyon De Ana, Kimberly A. Barker, Emad Arafa, Ian M. C. Martin, Joseph P. Mizgerd, Anna C. Belkina
Summary: Recovery from pneumococcal pneumonia leads to the development of tissue-resident memory lymphocytes in experienced lungs, providing protection against subsequent challenges. Utilizing full spectrum flow cytometry reveals unforeseen heterogeneity in the lung's adaptive immune landscape, including unexpected subsets of T-RM and B-RM cells. This study highlights the value of FSFC in confirmatory and discovery studies related to tissue resident adaptive immunity.
Article
Oncology
Thabet Alhousami, Michael Diny, Faiza Ali, Jennifer Shin, Gaurav Kumar, Vikas Kumar, Joshua D. Campbell, Vikki Noonan, Glenn J. Hanna, Gerald Denis, Stefano Monti, Maria A. Kukuruzinska, Xaralabos Varelas, Manish Bais
Summary: LSD1 inhibition suppresses the development of oral squamous cell carcinoma and shows a synergistic effect when combined with YAP inhibitor. Moreover, LSD1 inhibition upregulates the expression of PD-L1, leading to enhanced responses to immune checkpoint inhibitor therapy.
MOLECULAR CANCER RESEARCH
(2022)
Article
Dentistry, Oral Surgery & Medicine
Joao L. G. C. Monteiro, Toru Takusagawa, Belmiro C. E. Vasconcelos, Sara I. Pai, Joseph P. McCain, Fernando P. S. Guastaldi
Summary: This study aimed to describe rabbit temporomandibular joint (TMJ) anatomy and provide a minimally invasive approach for creating TMJ defects in order to investigate the regenerative potential of biomaterials. The surgical procedure had an average duration of 35.5 minutes with no major complications, demonstrating the feasibility of the model.
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY
(2022)
Editorial Material
Biology
Chinyere Kemet, Emily Hill, Hui Feng
Summary: Screening for drugs that disrupt embryonic development in zebrafish can aid in the identification of treatments that suppress metastasis.
Article
Biochemical Research Methods
Kim Vanuytsel, Anthony K. Yeung, Todd W. Dowrey, George J. Murphy, Anna C. Belkina
Summary: Hematopoietic stem cells have the ability to produce all mature blood cell types and maintain their population through self-renewing divisions. A multi-parameter spectral flow cytometry panel has been developed to better characterize HSC and their downstream progeny. In this study, this panel is used to comprehensively profile the HSC compartment in fetal liver.
Review
Oncology
Juhyun Oh, Tae Yeon Yoo, Talia M. Saal, Lisa Tsay, William C. Faquin, Jonathan C. T. Carlson, Daniel G. Deschler, Sara Pai, Ralph Weissleder
Summary: The authors developed a multiplexed cycling technology for the rapid analysis of single cells in salivary gland tumors, allowing for molecular analysis and diagnosis. They validated antibodies against 29 protein markers associated with different subtypes of salivary gland tumors, and developed a FAST molecular diagnostic assay that can distinguish between benign and malignant tumors. Using multiple markers increased the diagnostic accuracy compared to using a single marker.
CANCER CYTOPATHOLOGY
(2022)
Article
Oncology
Takanobu Shirai, Miho Sekai, Kei Kozawa, Nanami Sato, Nobuyuki Tanimura, Shunsuke Kon, Tomohiro Matsumoto, Takeru Murakami, Shoko Ito, Andrew Tilston-Lunel, Xaralabos Varelas, Yasuyuki Fujita
Summary: This study found that basal extrusion of single oncogenic mutant cells can induce a tumor microenvironment and epithelial-mesenchymal transition, generating characteristic precancerous lesions and providing molecular insights into the earlier steps of cancer development.
Editorial Material
Immunology
Paulin Brosinsky, Hanna Leister, Nan Cheng, Xaralabos Varelas, Alexander Visekruna, Maik Luu
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Oncology
Boting Ning, Andrew M. Tilston-Lunel, Justice Simonetti, Julia Hicks-Berthet, Adeline Matschulat, Roxana Pfefferkorn, Avrum Spira, Matthew Edwards, Sarah Mazzilli, Marc E. Lenburg, Jennifer E. Beane, Xaralabos Varelas
Summary: The study reveals the important gene regulatory roles of YAP/TAZ, TEADs, and TP63 in bronchial epithelial cells and premalignant lesions, which are closely associated with the development of lung squamous cell carcinoma. These factors promote basal epithelial cell proliferation and suppress signals related to interferon responses and immune cell communication. The findings provide molecular insights into the gene expression events driving premalignant lesion progression and offer potential therapeutic targets for lung cancer interception.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Biochemical Research Methods
Anthony Federico, Joseph Kern, Xaralabos Varelas, Stefano Monti
Summary: Network biology is a systems-based approach to better understand complex biological mechanisms. This article presents a novel approach to overcome challenges in reconstructing biological networks, by using biological organizing principles to limit model parameters and encourage shared learning. This method has been evaluated and shown promising results, especially in the analysis of tumor-specific networks and the identification of potential therapeutic targets for breast cancer.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Cell Biology
Fabrice J. F. Laroche, Sheng Li, Ning Shen, Soo Kyung Hwang, Gina Nguyen, Wenling Yu, Chen Khuan Wong, Ryan J. J. Quinton, Jason N. N. Berman, Ching-Ti Liu, Anurag Singh, Neil J. J. Ganem, Sam Thiagalingam, Hui Feng
Summary: Hyperactive sphingosine 1-phosphate (S1P) signaling is associated with poor prognosis of triple-negative breast cancer (TNBC). The phosphorylation of S1P receptor 1 (S1P1) at threonine 236 (T236) plays a critical role in TNBC dissemination and invasion. Inhibiting the phosphorylation of S1P1 T236 using AKT inhibitors or an S1P1 functional antagonist suppresses TNBC cell migration and tumor invasion.
Article
Cell & Tissue Engineering
Liang Ma, Bibek R. Thapa, Jake A. Le Suer, Andrew Tilston-Lunel, Michael J. Herriges, Andrew Berical, Mary Lou Beermann, Feiya Wang, Pushpinder S. Bawa, Anat Kohn, Alexandra B. Ysasi, Hirofumi Kiyokawa, Taylor M. Matte, Scott H. Randell, Xaralabos Varelas, Finn J. Hawkins, Darrell N. Kotton
Summary: In this study, the engraftment of airway epithelial stem cell compartment was successfully demonstrated through intra-airway transplantation of mouse or human primary and pluripotent stem cells. The engrafted cells showed stable morphologic, molecular, and functional phenotypes of airway epithelia for at least two years. This may pave the way for future cell-based therapy for diseases resulting from airway epithelial cell damage or dysfunction.
Article
Genetics & Heredity
Mohammed Muzamil Khan, Jennifer Frustino, Alessandro Villa, Bach-Cuc Nguyen, Sook-Bin Woo, William Evan Johnson, Xaralabos Varelas, Maria Kukuruzinska, Stefano Monti
Summary: Head and neck cancers, specifically oral cancer (OC), are highly deadly malignancies and early detection is lacking. A study on human oral premalignant lesions (PMLs) revealed gene signatures associated with cellular plasticity and immune response. Additionally, the study found associations between the oral microbiome and PML pathway activity, suggesting the potential contribution of oral microbiome to the progression of PMLs to oral squamous cell carcinoma (OSCC). These findings provide insights for early diagnosis and interception of the disease.
Review
Pathology
Ruben Bill, William C. Faquin, Sara I. Pai
Summary: Immune checkpoint inhibitors have improved the outcome of inoperable recurrent or metastatic head and neck squamous cell carcinoma. Predicting treatment response through biomarkers, particularly PD-L1 expression assessed by immunohistochemistry, is important for guiding clinical decision-making. This article discusses the clinical context, scoring methods, and impact on antibody approval related to PD-L1 expression, as well as the heterogeneity and technical considerations of determining this biomarker.
HEAD & NECK PATHOLOGY
(2023)