Article
Environmental Sciences
Shali Yu, Zhijie Li, Qin Zhang, Rui Wang, Zixuan Zhao, Wenjie Ding, Fengxu Wang, Chuan Sun, Juan Tang, Xiaoke Wang, Hongbing Zhang, Rongrong Huang, Qiyun Wu, Junkang Jiang, Xinyuan Zhao
Summary: This study reveals a novel mechanism by which antimony (Sb) triggers neurotoxicity through inducing ferroptosis in neurons by limiting the degradation of the antioxidant glutathione peroxidase 4 (GPX4). The study also found that inhibition of chaperone-mediated autophagy (CMA) can attenuate Sb-induced GPX4 degradation. These findings provide new insights into the understanding of Sb-induced neurotoxicity.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Fisheries
Jinjin Zhang, Wentao Wang, Shufei Liang, Rui Shao, Wenkai Shi, Gudmundur H. Gudmundsson, Peter Bergman, Qinghui Ai, Kangsen Mai, Min Wan
Summary: Short-chain fatty acids (SCFAs) promote the expression of IL-22 in fish macrophages, enhancing their bactericidal activity. Feeding turbot with sodium butyrate (NaB) reduces mortality after bacterial infection and increases IL-22 expression and bactericidal activity in macrophages. Treatment with NaB also promotes the expression of beta-defensins in zebrafish, although this effect is reduced in IL-22 mutant fish. Macrophages are identified as a cell source of IL-22 production in vivo.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Virology
Wei-Chung Chiou, Hsu-Feng Lu, Jui-Chieh Chen, Yu-Heng Lai, Ming-Fu Chang, Yuan-Li Huang, Ni Tien, Cheng Huang
Summary: This study identified a novel W box at the C terminus of HDV-III HDAg-L, which interacts with clathrin and may serve as a new molecular target for anti-HDV-III therapeutics.
Article
Entomology
Xuekai Shi, Shuai Li, Lin Yang, Xiaojian Liu, Hans Merzendorfer, Kun Yan Zhu, Jianzhen Zhang
Summary: In this study, a Chc ortholog was identified from a Locusta migratoria transcriptome database, and RNA interference experiments confirmed its important role in the development of L. migratoria, affecting growth, development, and reproduction.
Article
Multidisciplinary Sciences
Daniela Ivanova, Katharine L. Dobson, Akshada Gajbhiye, Elizabeth C. Davenport, Daniela Hacker, Sila K. Ultanir, Matthias Trost, Michael A. Cousin
Summary: The study reveals that regulating the copy number of the noncanonical vesicular SNARE protein VAMP4 on synaptic vesicles is crucial for controlling neurotransmitter release probability. VAMP4, with high synaptic turnover, is selectively sorted to endolysosomes during activity-dependent bulk endocytosis, disrupting which can lead to an increase in VAMP4 abundance in the SV pool and inhibition of SV fusion.
Article
Multidisciplinary Sciences
Tsuguto Masaki, Yoshio Kodera, Michishige Terasaki, Kazumi Fujimoto, Tsutomu Hirano, Masayoshi Shichiri
Summary: A new plasma peptidomic technique was used to identify a novel endogenous proatherogenic peptide, GIP_HUMAN[22-51], which accelerates atherosclerosis by modulating the cardiovascular system and inducing inflammatory responses.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Zhenzhu Jiang, Jing Yin, Guangyao Peng, Xiaoping Long
Summary: Circular RNA circ_0074027 was found to promote the proliferation and migration of NSCLC cells through the miR-362-3p/CLTC axis, while suppressing apoptosis.
Article
Agronomy
Da Xiao, Jianxiu Yao, Xiwu Gao, Kun Yan Zhu
Summary: Clathrin-dependent endocytosis is essential for insect development and reproduction in Tribolium castaneum. The knockdown of four major genes involved in this endocytic pathway resulted in larval mortality and deformed adults with wing development defects. The findings provide potential molecular targets for insect pest management strategies.
PEST MANAGEMENT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Sha Tang, Zhiying Zhao, Xiaotong Liu, Yi Sui, Dandan Zhang, Hui Zhi, Yuanzhu Gao, Hui Zhang, Linlin Zhang, Yannan Wang, Meicheng Zhao, Dongdong Li, Ke Wang, Qiang He, Renliang Zhang, Wei Zhang, Guanqing Jia, Wenqiang Tang, Xingguo Ye, Chuanyin Wu, Xianmin Diao
Summary: Researchers identify an E3 ligase SGD1 and its E2 partner responsible for grain yield control in foxtail millet, and reveal its conserved role in wheat, maize, and rice. Furthermore, SGD1 ubiquitinates the brassinosteroid receptor BRI1, thus stabilizing it and promoting grain yield in crops.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Bruno Sterlini, Alessandra Romei, Chiara Parodi, Davide Aprile, Michele Oneto, Anita Aperia, Pierluigi Valente, Flavia Valtorta, Anna Fassio, Pietro Baldelli, Fabio Benfenati, Anna Corradi
Summary: This study reveals that PRRT2 deficiency affects the function of Na+/K+ ATPase (NKA) in neurons, leading to increased clustering of NKA on the plasma membrane, which can be reversed by re-expression of PRRT2. Additionally, PRRT2 deficiency also reduces the NKA-dependent afterhyperpolarization in neurons following high-frequency firing.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Yuta Yamamura, Yasunori Iwata, Kengo Furuichi, Takahiro Kato, Naoki Yamamoto, Keisuke Horikoshi, Hisayuki Ogura, Koichi Sato, Megumi Oshima, Shiori Nakagawa, Taro Miyagawa, Shinji Kitajima, Tadashi Toyama, Akinori Hara, Norihiko Sakai, Miho Shimizu, Shinichi Horike, Takiko Daikoku, Ryuichi Nishinakamura, Takashi Wada
Summary: Kinesin family member 26b (Kif26b) is crucial for kidney development and plays an important role in the progression of renal fibrosis in adult settings. In a renal fibrosis model, Kif26b heterozygous mice showed protection against renal fibrosis. The study also found that Kif26b promotes migration and myofibroblast differentiation in renal fibroblasts through Myh9. Blocking this pathway could be a potential therapeutic approach for renal fibrosis.
Article
Biochemistry & Molecular Biology
Ryo Saito, Seisuke Mimori, Yasunobu Okuma, Koichi Kawada
Summary: The endoplasmic reticulum is crucial for intracellular quality control and ER stress can induce neuronal differentiation, with Sel1l playing an important role in this process.
NEUROCHEMICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Daisuke Saito, Yuko Nakagawa, Takashi Sato, Ayako Fukunaka, Ofejiro Blessing Pereye, Nobuhiro Maruyama, Hirotaka Watada, Yoshio Fujitani
Summary: In this study, we developed a method to specifically detect and measure the secretion of pancreatic polypeptide (PP), and investigated the molecular mechanisms that regulate PP secretion. We found that the secretion of PP is triggered by an increase in intracellular calcium concentrations via voltage-dependent calcium channels, unlike the mechanism of insulin secretion.
Article
Neurosciences
Carola I. Radulescu, Nazanin Doostdar, Nawal Zabouri, Leire Melgosa-Ecenarro, Xingjian Wang, Sadra Sadeh, Pavlina Pavlidi, Joe Airey, Maksym Kopanitsa, Claudia Clopath, Samuel J. Barnes
Summary: The study reveals dysregulation of neuronal activity control in the visual cortex of older adults, with downregulation of plasticity mechanisms leading to higher synaptic strengthening and activity levels following overstimulation. Overstimulation disrupts cognitive performance in older animals but not younger animals.
NATURE NEUROSCIENCE
(2023)
Article
Entomology
Hongshuang Wei, Shuqian Tan, Shuo Yan, Zhen Li, Jie Shen, Xiaoxia Liu
Summary: This study identified and cloned two genes, NPF1 and NPF2, from Grapholita molesta, revealing the important role of GmolNPF1 in regulating feeding behavior of the pest. Knockdown of GmolNPF1 using RNA interference significantly reduced larval feeding amount and survival rate. The combination of RNAi technology with dsRNA delivery system shows potential application in pest management.
JOURNAL OF PEST SCIENCE
(2022)
Article
Clinical Neurology
Michael S. Nahorski, Sateesh Maddirevula, Ryosuke Ishimura, Saud Alsahli, Angela F. Brady, Anaies Begemann, Tsunehiro Mizushima, Francisco J. Guzman-Vega, Miki Obata, Yoshinobu Ichimura, Hessa S. Alsaif, Shams Anazi, Niema Ibrahim, Firdous Abdulwahab, Mais Hashem, Dorota Monies, Mohamed Abouelhoda, Brian F. Meyer, Majid Alfadhel, Wafa Eyaid, Markus Zweier, Katharina Steindl, Anita Rauch, Stefan T. Arold, C. Geoffrey Woods, Masaaki Komatsu, Fowzan S. Alkuraya
Review
Cell Biology
Emma Lundberg, Georg H. H. Borner
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2019)
Article
Biology
Rolf M. Schmidt, Julia P. Schessner, Georg H. H. Borner, Sebastian Schuck
Article
Cell Biology
Anneri Sanger, Jennifer Hirst, Alexandra K. Davies, Margaret S. Robinson
JOURNAL OF CELL SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Robert Behne, Julian Teinert, Miriam Wimmer, Angelica D'Amore, Alexandra K. Davies, Joseph M. Scarrott, Kathrin Eberhardt, Barbara Brechmann, Ivy Pin-Fang Chen, Elizabeth D. Buttermore, Lee Barrett, Sean Dwyer, Teresa Chen, Jennifer Hirst, Antje Wiesener, Devorah Segal, Andrea Martinuzzi, Sofia T. Duarte, James T. Bennett, Thomas Bourinaris, Henry Houlden, Agathe Roubertie, Filippo M. Santorelli, Margaret Robinson, Mimoun Azzouz, Jonathan O. Lipton, Georg H. H. Borner, Mustafa Sahin, Darius Ebrahimi-Fakhari
HUMAN MOLECULAR GENETICS
(2020)
Review
Biochemical Research Methods
Georg H. H. Borner
MOLECULAR & CELLULAR PROTEOMICS
(2020)
Article
Cell Biology
Patrycja Kozik, Marine Gros, Daniel N. Itzhak, Leonel Joannas, Sandrine Heurtebise-Chretien, Patrycja A. Krawczyk, Pablo Rodriguez-Silvestre, Andres Alloatti, Joao Gamelas Magalhaes, Elaine Del Nery, Georg H. H. Borner, Sebastian Amigorena
Editorial Material
Biochemical Research Methods
Georg H. H. Borner
Article
Biochemistry & Molecular Biology
Lorena Martin-Jaular, Nathalie Nevo, Julia P. Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli, Damarys Loew, Kenneth W. Witwer, Matias Ostrowski, Georg H. H. Borner, Clotilde Thery
Summary: This study developed a proteomic profiling approach to study EV subtype composition and identified changes in cellular protein profiles in EVs upon HIV infection. The research also found that SERINC3 plays a role in controlling the surface composition of EVs.
Article
Multidisciplinary Sciences
Alexandra K. Davies, Julian E. Alecu, Marvin Ziegler, Catherine G. Vasilopoulou, Fabrizio Merciai, Hellen Jumo, Wardiya Afshar-Saber, Mustafa Sahin, Darius Ebrahimi-Fakhari, Georg H. H. Borner
Summary: The study identifies a potential mechanism for the childhood neurological disorder AP-4 deficiency syndrome, showing that the absence of AP-4 leads to defects in axonal growth. It is found that the enzyme responsible for producing the endocannabinoid 2-AG is not transported to the axon without AP-4, resulting in reduced levels of 2-AG and impaired axonal growth. The study also demonstrates that inhibiting the enzyme responsible for 2-AG breakdown rescues the growth defects in AP-4-deficient neurons.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Adam C. O'Neill, Fatma Uzbas, Giulia Antognolli, Florencia Merino, Kalina Draganova, Alex Jaeck, Sirui Zhang, Giorgia Pedini, Julia P. Schessner, Kimberly Cramer, Aloys Schepers, Fabian Metzger, Miriam Esgleas, Pawel Smialowski, Renzo Guerrini, Sven Falk, Regina Feederle, Saskia Freytag, Zefeng Wang, Melanie Bahlo, Ralf Jungmann, Claudia Bagni, Georg H. H. Borner, Stephen P. Robertson, Stefanie M. Hauck, Magdalena Goetz
Summary: The centrosome serves as an anchor for the cytoskeleton and plays a crucial role in cell division, migration, and cilia formation. By using spatial proteomics, researchers have uncovered protein interaction networks specific to the centrosome in human induced pluripotent stem cell-derived neural stem cells and neurons. These cell type-specific centrosome interactomes explain how genetic variants in widespread proteins can lead to brain-specific phenotypes.
Article
Multidisciplinary Sciences
Julia P. Schessner, Vincent Albrecht, Alexandra K. Davies, Pavel Sinitcyn, Georg H. H. Borner
Summary: The Dynamic Organellar Maps (DOMs) approach combines cell fractionation and shotgun-proteomics to analyze protein subcellular localization. By using data-independent acquisition (DIA) mass spectrometry, DIA-DOMs achieve deeper coverage and improve precision and reproducibility compared to previous methods. A software tool called DOM-ABC is introduced for analyzing profiling data. DIA-DOMs are applied to identify subcellular localization changes in HeLa cells, revealing cycles of Golgi proteins through endosomes. This workflow offers a superior label-free spatial proteomics approach for systematic phenotype discovery.
NATURE COMMUNICATIONS
(2023)
Article
Clinical Neurology
Darius Ebrahimi-Fakhari, Julian E. Alecu, Barbara Brechmann, Marvin Ziegler, Kathrin Eberhardt, Hellen Jumo, Angelica D'Amore, Parham Habibzadeh, Mohammad Ali Faghihi, Jan L. De Bleecker, Sandrine Vuillaumier-Barrot, Stephane Auvin, Filippo M. Santorelli, Sonja Neuser, Bernt Popp, Edward Yang, Lee Barrett, Alexandra K. Davies, Afshin Saffari, Jennifer Hirst, Mustafa Sahin
Summary: Adaptor protein complex 4-associated hereditary spastic paraplegia is caused by loss-of-function variants in AP4B1, AP4M1, AP4E1, or AP4S1. A functional assay using patient-derived fibroblasts to measure ATG9A subcellular localization has been established for diagnostic purposes, demonstrating robust diagnostic power and reliability in detecting disease-related variants.
BRAIN COMMUNICATIONS
(2021)