Article
Microbiology
Hai-Fan Zhao, Liang Meng, Zhi Geng, Zeng-Qiang Gao, Yu-Hui Dong, Hai-Wei Wang, Heng Zhang
Summary: This study reveals that phospholipids can bind to SVA 3C(pro) and regulate its protease activity, thereby affecting the replication and infection capacity of Seneca virus in host cells.
Article
Biochemistry & Molecular Biology
Yoann Abel, Christophe Charron, Camille Virciglio, Valerie Bourguignon-Igel, Marc Quinternet, Marie-Eve Chagot, Marie-Cecile Robert, Celine Verheggen, Christiane Branlant, Edouard Bertrand, Xavier Manival, Bruno Charpentier, Mathieu Rederstorff
Summary: In this study, it was found that the R2TP component RPAP3 binds to TRBP rather than PACT, and the binding of TRBP to RPAP3 or Dicer is mutually exclusive. Additionally, it was discovered that AGO(1/2), TRBP, and Dicer are all sensitive to HSP90 inhibition, with increased sensitivity of TRBP in the absence of RPAP3. Furthermore, RPAP3 appears to impede miRNA activity, suggesting that the R2TP chaperone might sequester TRBP to regulate the miRNA pathway.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Pascal Donsbach, Dagmar Klostermeier
Summary: RNA helicases are enzymes involved in various RNA metabolic processes, with a common core providing active and binding sites, regulated by multiple mechanisms. Case studies show how helicases with functions in translation, splicing, and RNA sensing are regulated in time and space.
BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Katarzyna Taylor, Agnieszka Piasecka, Arkadiusz Kajdasz, Aleksandra Brzek, Micaela Polay Espinoza, Cyril F. Bourgeois, Artur Jankowski, Malgorzata Borowiak, Katarzyna D. Raczynska, Lukasz J. Sznajder, Krzysztof Sobczak
Summary: This study aims to elucidate the mechanism of muscleblind-like splicing regulators (MBNLs) in alternative splicing (AS) processes. The findings indicate that DEAD-box (DDX) RNA helicases DDX5 and DDX17 play an important role in MBNL-dependent AS regulation. The helicases modulate MBNL splicing activity through changes in the ratio of MBNL splicing isoforms and accessibility of MBNL-binding sites. These findings contribute to a deeper understanding of the interdependencies between RNA-binding proteins and their implications in developmental homeostasis and pathological states.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jessica Cannavino, Mengle Shao, Yu A. An, Svetlana Bezprozvannaya, Shiuhwei Chen, Jiwoong Kim, Lin Xu, John R. McAnally, Philipp E. Scherer, Ning Liu, Rana K. Gupta, Rhonda Bassel-Duby, Eric N. Olson
Summary: The discovery of FAM195A, a BAT-enriched RNA binding protein, highlights its essential role in cold-dependent thermogenesis in mice. Knockout of FAM195A results in whitening of BAT and impaired thermoregulation, affecting the response to cold significantly.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Liyuan Gao, Zhitao Jiang, Yi Han, Yang Li, Xiang Yang
Summary: This review summarizes the regulation and function of non-coding RNA in cell pyroptosis and provides new insights for the study of pyroptosis in different diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Jianguo Feng, Jianlong Zhou, Yunxiao Lin, Wenhua Huang
Summary: This article highlights the importance of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) in regulating RNA metabolism and its dysregulation in various diseases. The selective binding sites of hnRNP A1 to RNA and DNA are summarized, along with the co-regulatory factors that interact with hnRNP A1. The potential therapeutic implications of targeting hnRNP A1 are discussed, including small-molecule drugs and biomedicines.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Dorian Farache, Sadie P. Antine, Amy S. Y. Lee
Summary: Translation factors not only play a role in protein synthesis, but also have additional functions in gene regulation, such as transcriptional control and extracellular signaling. These functions are highly regulated in response to external cues and the intrinsic cellular state, providing an additional mechanism for precise control of gene expression.
TRENDS IN CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ahmed M. Malik, Josephine J. Wu, Christie A. Gillies, Quinlan A. Doctrove, Xingli Li, Haoran Huang, Elizabeth H. M. Tank, Vikram G. Shakkottai, Sami Barmada
Summary: RNA-binding protein (RBP) dysfunction plays a fundamental role in amyotrophic lateral sclerosis (ALS) and related neuromuscular disorders. This study shows that activity-dependent processes regulate the levels and functions of the RBP Matrin 3 (MATR3). Glutamatergic activity drives MATR3 degradation, and mutations in MATR3 render it resistant to degradation, suggesting a link between activity-dependent MATR3 regulation and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Oncology
Morgane Le Bras, Noah Gorelick, Sylvain Pautet, Betty Tyler, Stephane Manenti, Nicolas Skuli, Stefania Millevoi, Anne Cammas
Summary: This study investigates the molecular mechanisms underlying protein synthesis deregulation in glioblastoma (GBM) and its impact on tumor progression and resistance to therapy. The results reveal the crucial role of RNA-binding proteins hnRNP H/F in controlling protein synthesis in GBM through overlapping mechanisms. Furthermore, hnRNP H/F are shown to potentiate cellular processes that contribute to the aggressive and resistant phenotype of GBMs, indicating their potential as therapeutic targets.
Article
Microbiology
Giraldina Trevejo-Nunez, Becky Lin, Li Fan, Felix E. Y. Aggor, Partha S. Biswas, Kong Chen, Sarah L. Gaffen
Summary: Excessive inflammation can cause tissue damage and autoimmunity. Reg1 deficiency exacerbates autoimmune diseases but confers resistance to Klebsiella pneumoniae (KP) by affecting type I interferon regulation.
Article
Biochemistry & Molecular Biology
Camila Pereira-Montecinos, Daniela Toro-Ascuy, Catarina Ananias-Saez, Aracelly Gaete-Argel, Cecilia Rojas-Fuentes, Sebastian Riquelme-Barrios, Barbara Rojas-Araya, Francisco Garcia-de-Gracia, Paulina Aguilera-Cortes, Jonas Chnaiderman, Monica L. Acevedo, Fernando Valiente-Echeverria, Ricardo Soto-Rifo
Summary: This study reveals the regulatory role of N-6-methyladenosine (m(6)A) deposition on HIV-1 full-length RNA packaging, as well as the interaction between HIV-1 Gag protein and RNA demethylase FTO. The results show that m(6)A deposition leads to increased Gag synthesis and reduced packaging, while FTO-mediated demethylation promotes the incorporation of the full-length RNA into viral particles.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Lilei Xu, Yu Xiao, Jie Zhang, Xianyang Fang
Summary: In bacteria, the expression of folate-related genes is controlled by different types of riboswitches, including the THF-I and recently discovered THF-II riboswitches. In this study, the crystal structures of THF-II riboswitch from Mesorhizobium loti were determined, revealing a unique architecture stabilized by continuous base pair stacking. The ligand binding pocket of the riboswitch accommodates the pterin moiety and its binding is influenced by Mg2+ ions. Ligand binding reduces accessibility of the ribosome binding site, regulating the expression of downstream genes. These findings provide mechanistic insights into translation regulation by THF-II riboswitch.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Tamar Szoke, Omer Goldberger, Nitsan Albocher-Kedem, Meshi Barsheshet, Nili Dezorella, Anat Nussbaum-Shochat, Reuven Wiener, Maya Schuldiner, Orna Amster-Choder
Summary: TmaR, the only known pole-localizer protein in Escherichia coli, assembles by phase separation via heterotypic interactions with RNA and controls flagella production, cell motility, and biofilm formation.
Article
Biochemistry & Molecular Biology
Lingyun Li, Kyrylo Krasnykov, David Homolka, Pascal Gos, Mateusz Mendel, Richard J. Fish, Radha Raman Pandey, Ramesh S. Pillai
Summary: The YTHDC2 protein plays a crucial role in mammalian fertility by binding to specific sites on the 3' UTRs of mouse testicular RNA targets. The loss of its 3'-> 5' RNA helicase activity leads to infertility in mice. Furthermore, its weak helicase activity is enhanced through interaction with the exoribonuclease XRN1. Studies on ythdc2 mutant zebrafish demonstrate its conserved role in animal germ cell development.
Article
Biochemistry & Molecular Biology
Nishita Parnandi, Veronic Rendo, Gaofeng Cui, Maria Victoria Botuyan, Michaela Remisova, Huy Nguyen, Pascal Drane, Rameen Beroukhim, Matthias Altmeyer, Georges Mer, Dipanjan Chowdhury
Summary: TIRR inhibits the formation of the 53BP1-p53 complex by preventing the recruitment of 53BP1 to DSBs, leading to aberrant p53 activity and affecting p53-mediated cell-fate programs.
Article
Biochemistry & Molecular Biology
Chloe Du Truong, Theodore A. Craig, Gaofeng Cui, Maria Victoria Botuyan, Rachel A. Serkasevich, Ka-Yi Chan, Georges Mer, Po-Lin Chiu, Rajiv Kumar
Summary: The study revealed that apo Pol zeta exhibits enhanced flexibility compared to its DNA-bound state, with concerted motions associated with expansion of the Pol zeta DNA-binding channel upon DNA binding. Additionally, a lysine residue obstructing the DNA-binding channel in apo Pol zeta was identified, suggesting a gating mechanism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Dejie Wang, Jian Ma, Maria Victoria Botuyan, Gaofeng Cui, Yuqian Yan, Donglin Ding, Yingke Zhou, Eugene W. Krueger, Jiang Pei, Xiaosheng Wu, Liguo Wang, Huadong Pei, Mark A. McNiven, Dingwei Ye, Georges Mer, Haojie Huang
Summary: The study demonstrates that SPOP facilitates HR repair over NHEJ by promoting the exclusion of 53BP1 from chromatin, contributing to the maintenance of genome integrity during DNA replication. This mechanism provides new insights into the choice between HR and NHEJ repair pathways in cells.
Article
Cell Biology
Zachary Mirman, Nanda Kumar Sasi, Ashleigh King, J. Ross Chapman, Titia de Lange
Summary: The primary function of the shieldin complex in double-strand break repair in BRCA1-deficient cells is to recruit the CST-Pol alpha-primase complex for fill-in synthesis. The effectiveness of poly(ADP)-ribose polymerase 1 inhibition (PARPi) in BRCA1-deficient cells depends on 53BP1 and shieldin, which limit single-stranded DNA at double-strand breaks through blocking resection and/or fill-in by CST-Pol alpha-primase. CST-Pol alpha-primase promotes radial chromosome formation and BrdU incorporation at DSBs in PARPi-treated BRCA1-deficient cells, and shieldin acts primarily by recruiting CST for radial formation.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jian Ma, Qing Shi, Gaofeng Cui, Haoyue Sheng, Maria Victoria Botuyan, Yingke Zhou, Yuqian Yan, Yundong He, Liguo Wang, Yuzhuo Wang, Georges Mer, Dingwei Ye, Chenji Wang, Haojie Huang
Summary: The study reveals that the CULLIN3 E3 ubiquitin ligase adaptor protein SPOP plays a crucial role in preventing DNA replication over-firing and genome instability by affecting Geminin ubiquitination. Mutations in SPOP may lead to replication origin over-firing, replication stress, and cell death, suggesting potential susceptibility to ATR inhibitor therapy in SPOP-mutated tumors.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Biomedical
Eloise Thomas, Clelia Mathieu, Patricia Moreno-Gaona, Vincent Mittelheisser, Francois Lux, Olivier Tillement, Xavier Pivot, Paiman Peter Ghoroghchian, Alexandre Detappe
Summary: A novel PET-based anti-BCMA nanoplatform labeled with Cu-64 has shown improved sensitivity and specificity in monitoring minute plasma cell populations in the spine and femur compared to traditional immunoPET approaches in detecting tumor cells in MM patients. This immuno-nanoPET platform has the potential to enhance the management of MM patients when combined with tumor biopsy.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Vincent Mittelheisser, Pierre Coliat, Eric Moeglin, Lilian Goepp, Jacky G. Goetz, Loic J. Charbonniere, Xavier Pivot, Alexandre Detappe
Summary: Tumor-targeted antibody/fragment-conjugated nanoparticles show promise in improving local delivery of small molecules. A meta-analysis of 161 studies confirmed that targeted nanoparticles had significantly higher tumor uptake compared to naked nanoparticles. The study also found that the choice of nanoparticle type, size, and antibody moiety affected tumor uptake.
ADVANCED MATERIALS
(2022)
Article
Clinical Neurology
Pitcha Chompoopong, Margherita Milone, Zhiyv Niu, Gaofeng Cui, Georges Mer, Teerin Liewluck
Summary: In this study, a novel variant in the HNRNPA1 gene was identified in a male patient with bilateral foot drop, expanding the phenotypic spectrum of hnRNPA1opathy.
NEUROMUSCULAR DISORDERS
(2022)
Article
Cell Biology
Micah J. McCauley, Michael Morse, Nicole Becker, Qi Hu, Maria Victoria Botuyan, Emily Navarrete, Ran Huo, Uma M. Muthurajan, Ioulia Rouzina, Karolin Luger, Georges Mer, James Maher, Mark C. Williams
Summary: The histone chaperone FACT enhances transcription by targeting DNA-protein interactions. This study demonstrates the impact of FACT on nucleosome stability and dynamics, revealing contradictory functions of different FACT subdomains in nucleosome remodeling. These findings provide insights into the catalytic role of key FACT domains in nucleosome disassembly and reassembly.
Editorial Material
Chemistry, Multidisciplinary
Qi Hu, Maria Victoria Botuyan, Georges Mer
Summary: The study presents a chemical trapping method to investigate short-lived intermediate states in substrate ubiquitylation. The effectiveness of this approach is demonstrated by determining single-particle cryo-EM structures related to nucleosome ubiquitylation.
Article
Multidisciplinary Sciences
Gaofeng Cui, Maria Victoria Botuyan, Pascal Drane, Qi Hu, Benoit Bragantini, James R. Thompson, David J. Schuller, Alexandre Detappe, Michael T. Perfetti, Lindsey I. James, Stephen V. Frye, Dipanjan Chowdhury, Georges Mer
Summary: This study reveals an autoinhibited state of 53BP1 that hinders its ability to bind to chromatin. Small molecule ligands present a potential avenue for the development of cancer therapy drugs.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Oncology
John Copland, Kendall Schick, Justyna Gleba, Truc Huynh, James Miller, Erin MIller, Aylin Alasonyalilar Demirer, Erin Tapper, Reona Sakemura, Elizabeth Siegler, Michelle Cox, Carli Stewart, Ismail Can, Ekene Ogbodo, Claudia Manriquez Roman, Bezerra Evandro, Cui Gaofeng, Mer Georges, Olivier Gloria, Yushi Qui, Robert Smallridge, Abba Zubair, Han Tun, Saad Kenderian
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Xiangyu Zeng, Fei Zhao, Gaofeng Cui, Yong Zhang, Rajashree A. Deshpande, Yuping Chen, Min Deng, Jake A. Kloeber, Yu Shi, Qin Zhou, Chao Zhang, Jing Hou, Wootae Kim, Xinyi Tu, Yuanliang Yan, Zhijie Xu, Lifeng Chen, Huanyao Gao, Guijie Guo, Jiaqi Liu, Qian Zhu, Yueyu Cao, Jinzhou Huang, Zheming Wu, Shouhai Zhu, Ping Yin, Kuntian Luo, Georges Mer, Tanya T. Paull, Jian Yuan, Kaixiong Tao, Zhenkun Lou
Summary: This study reveals the potential role of METTL16 in the treatment of PDAC, particularly in cases with high METTL16 expression. Combining PARP inhibitors with gemcitabine may be an effective treatment strategy.