Article
Materials Science, Multidisciplinary
Yue Wang, Xiujue Zheng, Jun Liu, Qixian Chen, Yan Zhao
Summary: The emergence of CRISPR-based genome editing systems has revolutionized the field of molecular biology and holds great promise for the treatment of various diseases. However, the lack of suitable delivery vehicles for effective intracellular delivery of CRISPR components limits its practical applications. In this study, a nanometer-scaled vesicle platform (CRISPRsome) was developed to efficiently deliver the essential components of the CRISPR system, resulting in significant gene knockout efficacy in cancer cells. This study highlights the potential of CRISPRsome in facilitating the use of CRISPR technology in practical applications.
ACS MATERIALS LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Huimin Ji, Wei Xiong, Shaoyuan Guo, Shaoru Wang, Xiwen Xing, Tian Tian, Xiang Zhou
Summary: A method involving a bioorthogonal cleavage reaction between isonitrile and tetrazine is proposed to modulate the cleavage activity of the CRISPR system. Isonitrile has significant potential for modifying sgRNAs, which has not been previously reported. Through post-synthetic modifications and click-and-release chemistry, the regulation of RNA-guided nucleic acid cleavage has been successfully achieved, holding great promise for controlling gene editing in human cells.
ACS CHEMICAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zituo Yang, Yepin Yu, Le Wang, Sek-Man Wong, Gen Hua Yue
Summary: The gene gab3 plays an important role in NNV replication and silencing gab3 can inhibit virus replication.
MARINE BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yan Zhang, Yupeng Cai, Shi Sun, Tianfu Han, Li Chen, Wensheng Hou
Summary: CRISPR/SaCas9 is an efficient genome editing tool that shows comparable suitability to SpCas9 in soybean, allowing for a wider range of target sequences for genome editing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)
Review
Horticulture
Zheng Wang, Shoujun Cao, Xinyang Xu, Yanjun He, Weisong Shou, Eduardo D. Munaiz, Chao Yu, Jia Shen
Summary: Increased consumption of vegetables is recommended worldwide, making it crucial to determine gene function for vegetable improvement. Virus-Induced Gene Silencing (VIGS) offers a rapid and large-scale approach to gene silencing in plants, providing opportunities for functional research. VIGS can accelerate model plant research and enhance gene analysis in vegetable crops, especially with the advancement of virus-mediated genetic tools. This study summarizes recent achievements in VIGS and Virus-Induced Gene Editing (VIGE) in vegetables and identifies challenges, serving as a guide for future research.
Article
Multidisciplinary Sciences
J. Boutin, J. Rosier, D. Cappellen, F. Prat, J. Toutain, P. Pennamen, J. Bouron, C. Rooryck, J. P. Merlio, I. Lamrissi-Garcia, G. Cullot, S. Amintas, V. Guyonnet-Duperat, C. Ged, J. M. Blouin, E. Richard, S. Dabernat, F. Moreau-Gaudry, A. Bedel
Summary: The study reveals the genotoxicity caused by CRISPR-Cas9 leading to loss of heterozygosity changes, while also highlighting the potential safety concerns of this technology for gene therapy.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Liangbing Yuan, Xin Song, Lu Zhang, Yaoguang Yu, Zhenwei Liang, Yawen Lei, Jiuxiao Ruan, Bin Tan, Jun Liu, Chenlong Li
Summary: The study reveals that the transcriptional repressors VAL1 and VAL2 recruit PRC2 to target loci in Arabidopsis thaliana. They physically interact with PRC2 catalytic subunits SWN and CLF, colocalize at target loci, and regulate H3K27me3 levels, affecting transcription.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Yan Liu, Yang Wang, Jiao Lin, Liang Xu
Summary: CRISPR/Cas9 function can be activated through ligand-induced approach by integrating the theophylline aptamer into protein-unrecognized regions of guide RNA. This allosteric regulation design offers a new perspective for the conditional control of CRISPR/Cas9 function through the involvement of RNA aptamers.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Guoxu Song, Fei Zhang, Chunhong Tian, Xing Gao, Xiaoxiao Zhu, Dongdong Fan, Yong Tian
Summary: This study identified novel anti-CRISPR families that have the ability to inhibit Cas9 activity and even control genome editing post-translationally. By studying different Acr variants and developing chemically inducible control, new avenues for CRISPR-Cas9 applications have been established.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Serene Rose David, Sumanth Kumar Maheshwaram, Divya Shet, Mahesh B. Lakshminarayana, Gautam Soni
Summary: This study investigates the binding properties of Cas9 enzyme to specific target DNA at different temperatures and finds that Cas9 enzyme can efficiently bind and cleave DNA at temperatures as low as 4 degrees C. The study also shows that the cleaved DNA products remain bound to the Cas9 enzyme strongly and are released only at higher temperatures. These findings provide valuable insights for developing temperature-dependent applications of the CRISPR-Cas9 technology.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Hadia Hussain, Yuxin Cheng, Yating Wang, Yuan Yuan, Yingying Li, Hainan Tian, Saddam Hussain, Siyu Chen, Rao Lin, Tianya Wang, Shucai Wang
Summary: The plant hormone abscisic acid (ABA) plays an important role in regulating plant responses to abiotic stresses by regulating the expression of ABA-responsive genes. ASR1 and ASR2 are serine-rich novel transcription repressors closely related to ABA responses in Arabidopsis. They negatively regulate ABA responses in a redundant manner.
Article
Chemistry, Multidisciplinary
Bora Jang, Hyejin Jang, Hyunsook Kim, Minjeong Kim, Michaela Jeong, Gyeong Seok Lee, Kyuri Lee, Hyukjin Lee
Summary: This study reports a universal chemical modification approach for Dicer substrate RNA nanostructures by optimizing protein-RNA interactions. The optimized RNA nanostructures showed excellent binding and processability with RNA binding proteins and significantly enhanced in vivo gene silencing activity.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biochemistry & Molecular Biology
Xin Zhang, Sihan Lv, Zhenhuan Luo, Yongfei Hu, Xin Peng, Jie Lv, Shanshan Zhao, Jianqi Feng, Guanjie Huang, Qin-Li Wan, Jun Liu, Hongxin Huang, Bing Luan, Dong Wang, Xiaoyang Zhao, Ying Lin, Qinghua Zhou, Zhen-Ning Zhang, Zhili Rong
Summary: CRISPR-mediated gene activation (CRISPRa) is a promising therapeutic gene editing strategy that can activate endogenous target genes in vivo. The compact and robust miniCas9 activator miniCAFE has shown potential for treating human diseases by activating longevity-regulating genes and regulating energy metabolism.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xinyu Ling, Liying Chang, Heqi Chen, Xiaoqin Gao, Jianhang Yin, Yi Zuo, Yujia Huang, Bo Zhang, Jiazhi Hu, Tao Liu
Summary: Researchers have improved the genome editing efficiency of the Cas12a system by covalently linking CRISPR RNA and Cas12a, achieving high-efficiency gene editing and providing a new approach for engineering other low-efficiency Cas family members.
Article
Engineering, Biomedical
Kristin M. Poole, Christopher E. Nelson, Rucha V. Joshi, John R. Martin, Mukesh K. Gupta, Sky Lar C. Haws, Taylor E. Kavanaugh, Melissa C. Skala, Craig L. Duvall
Review
Chemistry, Multidisciplinary
Samantha M. Sarett, Christopher E. Nelson, Craig L. Duvall
JOURNAL OF CONTROLLED RELEASE
(2015)
Article
Multidisciplinary Sciences
Christopher E. Nelson, Chady H. Hakim, David G. Ousterout, Pratiksha I. Thakore, Eirik A. Moreb, Ruth M. Castellanos Rivera, Sarina Madhavan, Xiufang Pan, F. Ann Ran, Winston X. Yan, Aravind Asokan, Feng Zhang, Dongsheng Duan, Charles A. Gersbach
Article
Engineering, Biomedical
Thomas A. Werfel, Corban Swain, Christopher E. Nelson, Kameron V. Kilchrist, Brian C. Evans, Martina Miteva, Craig L. Duvall
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2016)
Article
Biotechnology & Applied Microbiology
Christopher E. Nelson, Charles A. Gersbach
NATURE BIOTECHNOLOGY
(2016)
Review
Chemistry, Applied
Christopher E. Nelson, Charles A. Gersbach
ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING, VOL 7
(2016)
Article
Engineering, Biomedical
John R. Martin, Christopher E. Nelson, Mukesh K. Gupta, Fang Yu, Samantha M. Sarett, Kyle M. Hocking, Alonda C. Pollins, Lillian B. Nanney, Jeffrey M. Davidson, Scott A. Guelcher, Craig L. Duvall
ADVANCED HEALTHCARE MATERIALS
(2016)
Review
Clinical Neurology
Christopher E. Nelson, Jacqueline N. Robinson-Hamm, Charles A. Gersbach
NATURE REVIEWS NEUROLOGY
(2017)
Article
Multidisciplinary Sciences
Jianguo Huang, Mark Chen, Melodi Javid Whitley, Hsuan-Cheng Kuo, Eric S. Xu, Andrea Walens, Yvonne M. Mowery, David Van Mater, William C. Eward, Diana M. Cardona, Lixia Luo, Yan Ma, Omar M. Lopez, Christopher E. Nelson, Jacqueline N. Robinson-Hamm, Anupama Reddy, Sandeep S. Dave, Charles A. Gersbach, Rebecca D. Dodd, David G. Kirsch
NATURE COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Hongmei Li, Martina Miteva, Kellye C. Kirkbride, Ming J. Cheng, Christopher E. Nelson, Elaine M. Simpson, Mukesh K. Gupta, Craig L. Duvall, Todd D. Giorgio
Article
Biochemistry & Molecular Biology
Christopher E. Nelson, Yaoying Wu, Matthew P. Gemberling, Matthew L. Oliver, Matthew A. Waller, Joel D. Bohning, Jacqueline N. Robinson-Hamm, Karen Bulaklak, Ruth M. Castellanos Rivera, Joel H. Collier, Aravind Asokan, Charles A. Gersbach
Article
Biotechnology & Applied Microbiology
Adrian Pickar-Oliver, Joshua B. Black, Mae M. Lewis, Kevin J. Mutchnick, Tyler S. Klann, Kylie A. Gilcrest, Madeleine J. Sitton, Christopher E. Nelson, Alejandro Barrera, Luke C. Bartelt, Timothy E. Reddy, Chase L. Beisel, Rodolphe Barrangou, Charles A. Gersbach
NATURE BIOTECHNOLOGY
(2019)
Article
Oncology
Ariel Mundo, Abdussaboor Muhammad, Kerlin Balza, Christopher E. Nelson, Timothy J. Muldoon
Summary: Metronomic chemotherapy (MET) is a promising strategy to address the shortcomings of traditional maximum-tolerated chemotherapy (MTD) in terms of toxicity and resistance development. This study analyzed the impact of MET as neoadjuvant chemotherapy (NAC) in colorectal cancer (CRC) using a primary model. The results showed that MET and MTD NAC led to increased tissue oxygen saturation and oxyhemoglobin, but with distinct molecular mechanisms.
Review
Biophysics
Made Harumi Padmaswari, Shilpi Agrawal, Mary S. Jia, Allie Ivy, Daniel A. Maxenberger, Landon A. Burcham, Christopher E. Nelson
Summary: Duchene muscular dystrophy (DMD) is a neuromuscular disorder affecting male births, and gene editing, particularly CRISPR, shows promising potential for treating DMD.
BIOPHYSICS REVIEWS
(2023)