Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection

Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System

(2017) Weizhong Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System

(2016) Josef Altenbuchner   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Exploiting CRISPR–Cas immune systems for genome editing in bacteria

(2016) Rodolphe Barrangou et al.   CURRENT OPINION IN BIOTECHNOLOGY

In vivo high-throughput profiling of CRISPR–Cpf1 activity

(2016) Hui K Kim et al.   NATURE METHODS

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

(2016) Ákos Nyerges et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Next Generation Prokaryotic Engineering: The CRISPR-Cas Toolkit

(2016) Ioannis Mougiakos et al.   TRENDS IN BIOTECHNOLOGY

Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli

(2016) Lynn C. Thomason et al.   mBio

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase

(2015) Tao Xu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35

(2015) Zhaopeng Sun et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Application of Whole-Genome Sequencing for Bacterial Strain Typing in Molecular Epidemiology

(2015) Stephen J. Salipante et al.   JOURNAL OF CLINICAL MICROBIOLOGY

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Harnessing CRISPR–Cas systems for bacterial genome editing

(2015) Kurt Selle et al.   TRENDS IN MICROBIOLOGY

Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages

(2015) Ian R. Monk et al.   mBio

The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli

(2015) Chris R. Reisch et al.   Scientific Reports

Staphylococcus aureuschronic and relapsing infections: Evidence of a role for persister cells

(2014) Brian P. Conlon   BIOESSAYS

Role of Adaptor TrfA and ClpPC in Controlling Levels of SsrA-Tagged Proteins and Antitoxins in Staphylococcus aureus

(2014) N. P. Donegan et al.   JOURNAL OF BACTERIOLOGY

Igniting the Fire: Staphylococcus aureus Virulence Factors in the Pathogenesis of Sepsis

(2014) Michael E. Powers et al.   PLoS Pathogens

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Staphylococcus aureus virulence factors in evasion from innate immune defenses in human and animal diseases

(2013) Alfonso Zecconi et al.   IMMUNOLOGY LETTERS

Attenuating Staphylococcus aureus Virulence Gene Regulation: A Medicinal Chemistry Perspective

(2013) Christopher P. Gordon et al.   JOURNAL OF MEDICINAL CHEMISTRY

An update on the molecular genetics toolbox for staphylococci

(2013) M. Prax et al.   MICROBIOLOGY-SGM

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Staphylococcus aureus autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner

(2012) Dan Yu et al.   BMC MICROBIOLOGY

Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria

(2012) M. V. Larsen et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases

(2012) Joshua A. Mosberg et al.   PLoS One

Transforming the Untransformable: Application of Direct Transformation To Manipulate Genetically Staphylococcus aureus and Staphylococcus epidermidis

(2012) I. R. Monk et al.   mBio

Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus aureus

(2012) Kazuya Morikawa et al.   PLoS Pathogens

Exploring optimization parameters to increase ssDNA recombineering inLactococcus lactisandLactobacillus reuteri

(2012) Jan-Peter van Pijkeren et al.   Bioengineered

Expression of Virulence Factors by Staphylococcus aureus Grown in Serum

(2011) Yuichi Oogai et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Clinical Practice Guidelines by the Infectious Diseases Society of America for the Treatment of Methicillin-Resistant Staphylococcus aureus Infections in Adults and Children: Executive Summary

(2011) Catherine Liu et al.   CLINICAL INFECTIOUS DISEASES

Impact of rpoB Mutations on Reduced Vancomycin Susceptibility in Staphylococcus aureus

(2011) Yukiko Watanabe et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Probing Cellular Processes with Oligo-Mediated Recombination and Using the Knowledge Gained to Optimize Recombineering

(2011) James A. Sawitzke et al.   JOURNAL OF MOLECULAR BIOLOGY

Vectors for improved Tet repressor-dependent gradual gene induction or silencing in Staphylococcus aureus

(2011) L. Helle et al.   MICROBIOLOGY-SGM

Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion

(2011) H. H. Wang et al.   NUCLEIC ACIDS RESEARCH

Adaptive Evolution of Staphylococcus aureus during Chronic Endobronchial Infection of a Cystic Fibrosis Patient

(2011) Paul R. McAdam et al.   PLoS One

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

An improved tetracycline-inducible expression vector for Staphylococcus aureus

(2008) Rebecca M. Corrigan et al.   PLASMID

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

(2008) S. Datta et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA