期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 37, 期 -, 页码 61-68出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2015.10.003
关键词
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资金
- North Carolina State University
- University of Wisconsin-Madison
- Food Research Institute at UW-Madison
The CRISPR-Cas immune system is a DNA-encoded, RNA mediated, DNA-targeting defense mechanism, which provides sequence-specific targeting of DNA. This molecular machinery can be engineered into the sgRNA:Cas9 technology, for programmable cleavage of DNA. Following the genesis of double-stranded DNA breaks, the DNA repair machinery generates mutations at the cleavage site using various pathways. This technology has revolutionized eukaryotic genome editing, and we are at the cusp of full exploitation in bacteria. Here, we discuss the potential of CRISPR-based technologies for use in bacteria, and highlight the application of single stranded DNA recombineering combined with CRISPR-Cas selection to edit the genome of a probiotic organism. We envision that CRISPR-Cas technologies will play a key role in the development of next-generation industrial bacteria.
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