Article
Fisheries
Qing Nie, Jiaoyang Xia, Weihong Zhao, Qingqing Yan, Bin Geng, Guo Qiao, Fu Lv, Linlan Lv, Fengjuan Jiang, Longyu Liu
Summary: In this study, a pathogenic bacterium was isolated from the Oriental river prawn with a red hepatopancreas and identified as a member of the Bacillus cereus group. The bacterium exhibited virulence genes and sensitivity to certain antibiotics, providing a theoretical basis for clinical treatment and prevention of diseases caused by this strain in the Oriental river prawn.
AQUACULTURE RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Marketa Nykrynova, Vojtech Barton, Matej Bezdicek, Martina Lengerova, Helena Skutkova
Summary: The study introduces a pipeline for identifying highly variable genomic fragments in unmapped reads through a modified hybrid assembly approach. These variable regions can be used in efficient laboratory methods for bacterial typing with high discriminatory power, such as mini-MLST, replacing expensive methods like MLST. Through this approach, infection monitoring can be carried out more rapidly.
Article
Biochemistry & Molecular Biology
Tomasz Wolkowicz, Katarzyna Zacharczuk, Aleksandra Anna Zasada
Summary: Infections caused by non-toxigenic Corynebacterium diphtheriae have been increasing in Poland, with the most common strains being ST8 biovar gravis. This study analyzed 30 strains isolated between 2017 and 2022, as well as six previously isolated strains, using classic methods and whole genome sequencing. The appearance of new strains in 2022 and the isolation of the first non-toxigenic tox gene-bearing strain suggest that C. diphtheriae should be considered a pathogen of special public health concern in Poland.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Cecilia Plesken, Patrick Pattar, Bianka Reiss, Zinnia Naoshin Noor, Lisha Zhang, Klaus Klug, Bruno Huettel, Matthias Hahn
Summary: Botrytis cinerea, a plant pathogen causing gray mold rot on various crops, was studied using a multilocus sequence typing (MLST) scheme to evaluate genetic diversity. Through MLST analysis, high-resolution strain differentiation and robust species separation were achieved. The study revealed unknown populations of B. cinerea and genetically distinct strains on Iris pseudacorus.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Madikay Senghore, Hannah Read, Priyali Oza, Sarah Johnson, Hemanoel Passarelli-Araujo, Bradford P. Taylor, Stephen Ashley, Alex Grey, Alanna Callendrello, Robyn Lee, Matthew R. Goddard, Thomas Lumley, William P. Hanage, Siouxsie Wiles
Summary: Identifying and interrupting transmission chains is crucial for controlling infectious diseases. This study assesses the use of deep-sequenced genomic surveillance to identify transmission pairs and highlights the importance of within-host variation in transmission.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Barbara M. Binney, Hayley Pragert, Jonathan Foxwell, Edna Gias, Meredith L. Birrell, Bernard J. Phiri, Oliver Quinn, Michael Taylor, Hye Jeong Ha, Richard J. Hall
Summary: New Zealand, a remote country in the South Pacific Ocean, introduced honey bees in 1839, which subsequently led to the discovery of American foulbrood in the 1870s. The genome sequencing of Paenibacillus larvae obtained from 164 New Zealand apiaries revealed that ST18 was the dominant sequence type, accounting for 90.2% of isolates.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Kristyna Dufkova, Matej Bezdicek, Marketa Nykrynova, Iva Kocmanova, Petra Kubackova, Jana Hanslikova, Karolina Fejkova, Jiri Mayer, Martina Lengerova
Summary: In this study, a novel mini-MLST typing scheme for Pseudomonas aeruginosa was designed. It has great discriminatory power, ease of performance, and short processing time, making it suitable for prospective typing of large isolate sets. Integrating the novel typing scheme enables the investigation of the development and spread of multidrug-resistant/extensively drug-resistant high-risk clones.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Hongxuan Li, Shiqian Fu, Danliangmin Song, Xue Qin, Wei Zhang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: In this study, 35 Cronobacter strains isolated from powdered infant formula and its processing environment were identified and typed using 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 different sequence types were obtained, including three new ones that were isolated for the first time. The antibiotic resistance analysis revealed that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Moreover, 68.57% of the strains showed multi-drug resistance, with the most drug-resistant Cronobacter strain exhibiting resistance to 13 different drugs. Transcriptomics analysis identified 77 differentially expressed genes related to drug resistance, and the study further revealed that Cronobacter strains can activate multidrug efflux systems to enhance drug resistance. Understanding the drug resistance and mechanisms of Cronobacter is of great public health significance for the rational selection and development of antibacterial drugs, as well as the control and treatment of infections caused by this pathogenic microorganism.
Article
Food Science & Technology
Hua Jiang, Yue Xiang, Xiaojuan He, Chencheng Li, Fuxing Lin, Jihong Shao, Yuanhong Li
Summary: This study investigated the prevalence and characterization of Cronobacter spp. in dried edible mushrooms in Jiangsu province, China. The overall prevalence of Cronobacter spp. in dried edible mushrooms was 14.8%, with high genetic diversity observed. Some Cronobacter strains showed resistance to certain antibiotics, although they were susceptible to most tested antibiotics. Most isolates showed weak biofilm formation ability. This study provides new epidemiological evidence for the presence of Cronobacter spp. in dried edible mushrooms.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Microbiology
Lu Wang, Pan Wu, Yingying Su, Yi Wei, Xi Guo, Lan Yang, Min Wang, Bin Liu
Summary: This study identified novel marker genes for the genus Cronobacter, as well as specific marker genes for three species within the genus. Duplex and multiplex PCR methods based on these markers were established and validated for accurate detection of the strains. The developed methods can be used as valuable tools for identifying these strains in various samples.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jiri Novotny, Barbora Svobodova, Jiri Santrucek, Ladislav Fukal, Ludmila Karamonova
Summary: This study presents an improved method for mapping the proteome of Cronobacter sakazakii, a genus responsible for severe infections. By fractionating the bacterial cells and using a novel protein identification approach, they identified a large number of proteins, providing important insights into the pathogenesis and virulence factors of this bacterium.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Zhenyu Wang, Haiyan Xu, Chao Chu, Yuanyue Tang, Qiuchun Li, Xinan Jiao
Summary: The monophasic variant of Salmonella Typhimurium has rapidly increased worldwide in the past twenty years, characterized by the loss of second-phase flagella genes and acquisition of multi-drug resistance. Two Salmonella strains isolated from a 4-year-old girl presented with septic arthritis were identified as a novel monophasic variant through whole-genome sequencing analysis, revealing a unique antimicrobial resistance mechanism and genetic relationship to other strains in different countries.
Article
Microbiology
Henrik Christensen, Sajid Mahmood Sajid, Magne Bisgaard, Chiara Francesca Magistrali, Francesca Romana Massacci, Martin Liman, Theresa Menke, Henning Bischoff, John E. Olsen
Summary: This study predicted the serotypes of Pasteurella multocida based on whole genomic sequences and validated the predictions by comparing with classical phenotypic typing. The results showed that the serotypes of Pasteurella multocida can be effectively predicted using whole genomic sequences.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Xiaorong Zhang, Yang Chen, Di Xie, Mengjiao Guo, Shuang Ma, Mengyao Chen, Dianfeng Chu, Yantao Wu
Summary: The study found that Chinese M. synoviae strains have new allelic sequences, indicating potential new sequence types. All isolates were classified into group 11, which is independent from other reference strains in the PubMLST database.
VETERINARY MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Maira Jalil, Fatima Quddos, Farha Anwer, Samavi Nasir, Abdur Rahman, Metab Alharbi, Abdulrahman Alshammari, Huda Kamel Alshammari, Amjad Ali
Summary: This study conducted a comprehensive pangenome and phylogenetic analysis of S. aureus genomes to understand its species divergence. The current MLST scheme was evaluated and revised, incorporating new candidate genes to improve the resolution of strain typing. The proposed MLST method offers a greater resolution with 58 sequence types (STs) compared to the previous scheme's 42 STs.
