Article
Microbiology
Nicole Brimer, Scott Vande Pol
Summary: This study reveals that the enhanced competitiveness of HPV16-infected cells is primarily due to the expression of the E6 oncoprotein, rather than the E7 or E5 oncoproteins. E6 can induce a super-competitor phenotype, which has important implications for understanding the mechanism of cell competition.
Article
Biochemistry & Molecular Biology
Ethan L. Morgan, James A. Scarth, Molly R. Patterson, Christopher W. Wasson, Georgia C. Hemingway, Diego Barba-Moreno, Andrew Macdonald
Summary: The study identified activation of the JNK signaling pathway in cervical disease and cancer, with the HPV E6 oncogene inducing JNK1/2 phosphorylation. Blocking JNK signaling reduced cell proliferation and induced apoptosis, while promoting EGFR signaling. This positive feedback loop between EGFR signaling and HPV E6/E7 expression may offer a novel therapeutic target for cervical cancer treatment.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Medical Laboratory Technology
Xin Yang, Hai-Yang Liao, Hai-Hong Zhang
Summary: The MET gene plays a significant role in the initiation and development of various human cancers, affecting cell proliferation, migration, and invasion. MET serves as a biomarker for diagnosis and prognosis, and is also a potential therapeutic target. Inhibiting MET expression and function may have clinical benefits in cancer treatment.
CLINICA CHIMICA ACTA
(2022)
Article
Oncology
Nima Hemmat, Mohammad Amin Doustvandi, Zahra Asadzadeh, Ahad Mokhtarzadeh, Behzad Baradaran, Hossein Bannazadeh Baghi
Summary: The study demonstrates that silencing the E5 protein in cervical cancer cells can slow down cell proliferation and increase apoptosis, which may help inhibit the progression of cervical cancer.
Article
Microbiology
Ramon Antano-Arias, Oscar Del Moral-Hernandez, Julio Ortiz-Ortiz, Luz Del Carmen Alarcon-Romero, Jorge Adan Navor-Hernandez, Marco Antonio Leyva-Vazquez, Marco Antonio Jimenez-Lopez, Jorge Organista-Nava, Berenice Illades-Aguiar
Summary: This study identified specific E7 variants associated with the highest risk of cervical cancer, with E6/E7 bicistron analysis revealing even greater risk potential for specific variants. These findings underscore the importance of analyzing combinations of E6/E7 variants in HPV 16 infection for predicting cervical cancer development.
Article
Biochemistry & Molecular Biology
Magdalena Millan, Lihuen Villarreal, Natali D'Aiuto, Ronell Bologna-Molina, Jose Sotelo-Silveira, Juan C. Benech, Jimena Hochmann, Miguel Arocena
Summary: During tumorigenesis, the mechanical properties of cancer cells change, with decreased stiffness often accompanying a more invasive phenotype. A recent study used atomic force microscopy to measure cell stiffness and observed a decrease in Young's modulus and cell rigidity in the pre-tumoral cell model of cervical cancer. This study suggests that decreased stiffness and perturbation in cell shape are early mechanical and morphological changes during malignant transformation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Diego Carrillo-Beltran, Julio C. Osorio, Rances Blanco, Carolina Oliva, Enrique Boccardo, Francisco Aguayo
Summary: Although epidemiological studies suggest that tobacco smoking and HR-HPV infection are mutually exclusive risk factors for developing HNC, some individuals are both HPV-positive and smokers. Both factors increase oxidative stress and DNA damage. This study found that oral cells expressing HPV16 E6/E7 oncoproteins exposed to cigarette smoke condensate showed synergistic increases in SOD2 levels and DNA damage, independent of Akt1 and ATM. These findings suggest that the interaction between HPV and cigarette smoke in HNC promotes SOD2 alterations, leading to increased DNA damage and the development of a distinct clinical entity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Jiajian Wang, Jie Dong, Yinjian Zhou, Kaiyi Wang, Min Pan, Zaixing Deng, Pengfei Wang, Ying Du, Weiguo Lu
Summary: This study aimed to assess the clinical performance of an HPV E6/E7 mRNA assay (Aptima HPV, AHPV) and AHPV 16 18/45 genotype assay (AHPV-GT) combined with age stratification for triaging women with atypical squamous cells of undetermined significance (ASC-US) cytology. The results showed that AHPV testing with age stratification is effective for triaging women with ASC-US cytology, and AHPV-GT testing may be a proper risk stratification method for women with AHPV-positive ASC-US cytology.
GYNECOLOGIC ONCOLOGY
(2022)
Article
Virology
Christine Kottaridi, Panagiota Resta, Danai Leventakou, Katerina Gioti, Ioannis Zygouras, Alina-Roxani Gouloumi, Georgios Sakagiannis, Khalid J. Alzahrani, Maria S. Venetikou, Fragkiski Anthouli-Anagnostopoulou, Apostolos Beloukas
Summary: Recent trends show a significant increase in the incidence of oropharyngeal squamous cell carcinoma associated with high-risk HPV type 16. However, there is limited published data on the genetic variations of this HPV type in oropharyngeal cancer. In this study, the genetic variations of HPV16 E6 gene sequences from a small cohort of Greek patients diagnosed with oropharyngeal cancer were assessed. The majority of sequences belonged to the European variant branch, with the T350G variation being the most predominant. Further studies are needed to explain the potential impact of this variation in the pathomechanisms of oropharyngeal cancer.
Article
Virology
M. Elina Totaro, Juan A. Gili, D. Javier Liotta, Theodore G. Schurr, Maria A. Picconi, Ines Badano
Summary: The province of Misiones is considered a region with a high mortality rate due to cervical cancer. Genetic variation in the E6 350G gene of HPV16 is associated with an increased risk of CC in this region. Phylogenetic analysis shows the presence of Lineage A and D subtypes in the samples.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Microbiology
James C. Romero-Masters, Laura K. Muehlbauer, Mitchell Hayes, Miranda Grace, Evgenia Shishkova, Joshua J. Coon, Karl Munger, Paul F. Lambert
Summary: The E6 protein encoded by MmuPV1 has potent oncogenic activities in mouse keratinocytes, including inhibition of differentiation, promotion of proliferation, and decreased dependence on growth factors. Proteomic and transcriptomic analyses revealed that MmuPV1 E6 upregulates the expression of proteins related to cellular proliferation and other cancer hallmarks. These findings indicate that MmuPV1 E6 is a major driver of MmuPV1-induced pathogenesis.
Article
Virology
Kerry Dust, Michael Carpenter, Julie Chih-yu Chen, Chris Grant, Stuart McCorrister, Garret R. Westmacott, Alberto Severini
Summary: This study investigates the effects of HPV16 viral proteins E6 and E7 on the host proteome in human neonatal keratinocytes. The results show that the expression of E6 and E7 leads to dysregulation in pathways related to interferon response, inflammation, and DNA damage repair. The study also identifies potential biomarkers associated with HPV oncoproteins in primary keratinocytes.
Article
Oncology
Wenjun Yuan, Shuying Li, Jun Jia, Li Wang, Yuchao Huang, Minglian Wang, Fei Xie, Jintao Li, Yanzhe Hao
Summary: This study found that HPV infection, especially HPV16 and HPV18, may be a risk factor for esophageal cancer in the Chinese population. The E6 protein may play a key role in HPV-associated malignancies. These findings are important for the prevention and treatment of HPV-positive esophageal cancer in China.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Virology
Shuo Li, Mengxia Ye, Yonglin Chen, Quan Gong, Bing Mei
Summary: This study sheds light on the relationship between HPV-52 and cervical cancer, identifying variations in the E6-E7 genes and analyzing the protein's structure and selective pressure. Multiple single nucleotide variants were found in the E6 and E7 sequences of HPV-52, some impacting B cell epitopes.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Pharmacology & Pharmacy
Yang Zhao, Wenling Ye, Yan-Dong Wang, Wei-Dong Chen
Summary: Hepatocyte growth factor (HGF) is a multifunctional cytokine that regulates cell growth, proliferation, and survival by activating the c-Met signaling pathway, and it plays a crucial role in liver regeneration.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ximena Maria Muresan, Marie-Sophie Narzt, Brittany Woodby, Francesca Ferrara, Florian Gruber, Giuseppe Valacchi
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Virology
Gaurav Raikhy, Brittany L. Woodby, Matthew L. Scott, Grace Shin, Julia E. Myers, Rona S. Scott, Jason M. Bodily
JOURNAL OF VIROLOGY
(2019)
Article
Biochemistry & Molecular Biology
Francesca Ferrara, Erika Pambianchi, Alessandra Pecorelli, Brittany Woodby, Nicolo Messano, Jean-Philippe Therrien, Mary Ann Lila, Giuseppe Valacchi
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Virology
Matthew L. Scott, Brittany L. Woodby, Joseph Ulicny, Gaurav Raikhy, A. Wayne Orr, William K. Songock, Jason M. Bodily
JOURNAL OF VIROLOGY
(2020)
Article
Virology
J. E. Myers, J. T. Guidry, M. L. Scott, K. Zwolinska, G. Raikhy, K. Prasai, M. Bienkowska-Haba, J. M. Bodily, M. J. Sapp, R. S. Scott
Article
Environmental Sciences
Franco Cervellati, Mascia Benedusi, Francesco Manarini, Brittany Woodby, Mara Russo, Giuseppe Valacchi, Maria Chara Pietrogrande
Article
Nutrition & Dietetics
David C. Nieman, Giuseppe Valacchi, Laurel M. Wentz, Francesca Ferrara, Alessandra Pecorelli, Brittany Woodby, Camila A. Sakaguchi, Andrew Simonson
INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM
(2020)
Review
Food Science & Technology
Brittany Woodby, Kayla Penta, Alessandra Pecorelli, Mary Ann Lila, Giuseppe Valacchi
ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 11
(2020)
Article
Biochemistry & Molecular Biology
Brittany Woodby, Claudia Sticozzi, Erika Pambianchi, Gino Villetti, Maurizio Civelli, Giuseppe Valacchi, Fabrizio Facchinetti
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Biochemistry & Molecular Biology
Alessandra Pecorelli, Francesca Ferrara, Nicolo Messano, Valeria Cordone, Maria Lucia Schiavone, Franco Cervellati, Brittany Woodby, Carlo Cervellati, Joussef Hayek, Giuseppe Valacchi
Article
Environmental Sciences
Brittany Woodby, Maria Lucia Schiavone, Erika Pambianchi, Angela Mastaloudis, Shelly N. Hester, Steven M. Wood, Alessandra Pecorelli, Giuseppe Valacchi
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Biochemistry & Molecular Biology
Francesca Ferrara, Brittany Woodby, Alessandra Pecorelli, Maria Lucia Schiavone, Erika Pambianchi, Nicolo' Messano, Jean-Philippe Therrien, Hina Choudhary, Giuseppe Valacchi
Article
Biochemistry & Molecular Biology
Brittany Woodby, Erika Pambianchi, Francesca Ferrara, Jean-Philippe Therrien, Alessandra Pecorelli, Nicolo' Messano, Mary Ann Lila, Giuseppe Valacchi
Summary: Exposure to O3 is reported to affect AMP levels in the skin, playing a role in skin inflammatory conditions, indicating a possible defensive strategy against pollution-associated skin conditions.
Article
Biochemistry & Molecular Biology
Alexandra C. Finney, Matthew L. Scott, Kaylea A. Reeves, Dongdong Wang, Mabruka Alfaidi, Jake C. Schwartz, Connor M. Chitmon, Christina H. Acosta, James M. Murphy, J. Steven Alexander, Christopher B. Pattillo, Ssang-Taek Lim, A. Wayne Orr
Summary: The study reveals that EphA2 plays a crucial role in regulating fibrillar adhesion elongation and its deficiency affects fibronectin deposition by influencing cell contractility and RhoA signaling.
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.