Article
Chemistry, Multidisciplinary
Milica Veljkovic, Milica Simovic, Katarina Banjanac, Marija Corovic, Ana Milivojevic, Milan Milivojevic, Dejan Bezbradica
Summary: In this study, a heterofunctional carrier was obtained by modifying a macroporous polystyrene ion exchange resin, which enabled the covalent immobilization of fructosyltransferase for the synthesis of functionally active fructo-oligosaccharides. The developed immobilized preparation exhibited significantly higher activity compared to the commercial preparation and achieved successful continuous production of fructo-oligosaccharides in an air-lift reactor.
REACTION CHEMISTRY & ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Thays N. da Rocha, Roberto Morellon-Sterlling, Javier Rocha-Martin, Juan M. Bolivar, Luciana R. B. Goncalves, Roberto Fernandez-Lafuente
Summary: Penicillin G acylase from Escherichia coli was successfully immobilized on vinyl sulfone agarose beads. The immobilization was optimized with the purpose of obtaining a stable and active biocatalyst. The study found that vinyl sulfone agarose beads have heterofunctionality, offering new possibilities for enzyme immobilization.
Article
Biochemistry & Molecular Biology
Javier Santiago-Arcos, Susana Velasco-Lozano, Fernando Lopez-Gallego
Summary: Immobilized multienzyme systems were successfully achieved using a heterofunctional support activated with three different chemical functionalities. The support, based on agarose microbeads activated with aldehyde, amino, and cobalt chelate moieties, enabled fast and irreversible immobilization of enzymes, resulting in enhanced thermostability (up to 21-fold higher) compared to soluble enzymes. Moreover, this trifunctional support efficiently coimmobilized a multienzyme system consisting of alcohol dehydrogenase, reduced nicotinamide adenine dinucleotide (NADH) oxidase, and catalase, exhibiting higher performance than its free counterpart with a total turnover number (TTN) of 1 x 105 during five batch consecutive cycles. This solid material holds promise as a platform for coimmobilizing multienzyme systems with enhanced properties for stepwise biotransformations.
Article
Chemistry, Physical
Thays N. da Rocha, Roberto Morellon-Sterling, Luciana R. B. Goncalves, Juan M. Bolivar, Andres R. Alcantara, Javier Rocha-Martin, Roberto Fernandez-Lafuente
Summary: In this study, MANAE-vinyl sulfone (VS) agarose beads were found to be a better support for immobilizing PGA enzyme compared to agarose-VS beads. The modified beads allowed for rapid and covalent immobilization of PGA, resulting in improved stability compared to low ionic strength VS-agarose.
Article
Chemistry, Multidisciplinary
Ana Benitez-Mateos, Francesca Paradisi
Summary: A novel halotolerant purine nucleoside phosphorylase from Halomonas elongata (HePNP) was used to achieve high conversion rates in the synthesis of valuable nucleoside drugs, along with an immobilization strategy on agarose microbeads and a catch-and-release approach for recycling excess sugar donors. This study demonstrated a high reproducibility and increased sustainability of the process, while also enhancing cost-efficiency.
Review
Biochemistry & Molecular Biology
F. Rafiee, M. Rezaee
Summary: Immobilized enzymes, particularly lipase on chitosan support, have attracted significant attention due to their advantages in industry and biochemistry. This review highlights the recent studies on lipase immobilization and discusses the potential solutions for current limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Veymar G. Tacias-Pascacio, Roberto Morellon-Sterling, Daniel Castaneda-Valbuena, Angel Berenguer-Murcia, Majid Rasool Kamli, Olga Tavano, Roberto Fernandez-Lafuente
Summary: This paper reviews the immobilization of papain on different supports for the first time, with a special focus on the preparation of magnetic biocatalysts and specific problems related to the immobilization of proteases. The benefits of proper immobilization and potential artifacts are discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biotechnology & Applied Microbiology
Soumya Koippully Manikandan, Dimitrios A. Giannakoudakis, Jovana R. Prekodravac, Vaishakh Nair, Juan Carlos Colmenares
Summary: This article focuses on the use of material supports for developing immobilized biocatalysts in various applications. Different types of materials, including organic, inorganic, and composites, have been shown to be effective for supporting the immobilization of enzymes and microbial cells. Nanomaterial supports offer advantages such as large surface area and comfortable compartments for immobilization. The choice of catalyst support depends on the interaction between the material and the enzyme or microbial cell.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chan Hee Lee, Hye Sun Lee, Jae Won Lee, Jangyong Kim, Jin Hyung Lee, Eon Seon Jin, Ee Taek Hwang
Summary: Enzyme immobilization using inorganic materials, such as CaCO3, has been shown to improve enzyme stability and activity. In this study, in situ and crosslink-immobilized enzymes in a CaCO3 carrier were compared, with both methods preserving over 30% of initial enzyme activity after 180 days. However, differences in enzyme loading, activity, and characteristics were observed between the two methods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Green & Sustainable Science & Technology
Marziyeh Khozeymeh Nezhad, Hamidreza Aghaei
Summary: In this study, Cloisite 30B was converted to tosylated Cloisite as a new heterofunctional support for immobilizing lipase to produce biodiesel efficiently. The results showed that under certain conditions, TCLL can catalyze biodiesel production efficiently, with high stability and reusability.
Article
Biochemistry & Molecular Biology
Kangjing Wang, Liting Zhao, Ting Li, Qian Wang, Zhongyang Ding, Weifu Dong
Summary: The application of Ni-chelated ion exchange resin in enzyme immobilization and protein purification is reported in this paper. Acrylic weak acid cation exchange resin (D113H) was selected as the carrier resin, and phosphomannose isomerase (PMI) was successfully immobilized on Ni-chelated D113H resin. The immobilized enzyme showed excellent reusability and retained 92% of its initial activity. Additionally, PMI was successfully purified using an affinity chromatography column prepared by Ni-chelated D113H resin, indicating the potential for combining immobilization and purification in one step.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Sahar Zahirinejad, Roohullah Hemmati, Ahmad Homaei, Ali Dinari, Saman Hosseinkhani, Soheila Mohammadi, Fabio Vianello
Summary: This study found that organic nanomaterials have a significant improvement on enzyme immobilization, increasing enzyme stability and reusability while protecting against harmful metal ions. Organic nanomaterials also have environmental advantages in medical applications, and are expected to be widely used as novel nano-supports for enzyme immobilization.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Diego Carballares, Roberto Fernandez-Lafuente, Javier Rocha-Martin
Summary: This paper presents different methodologies for the immobilization of D-amino acid oxidase (DAAO). The results suggest that immobilization on glutaraldehyde support at pH 7 provides the best stability and activity for the enzyme.
PROCESS BIOCHEMISTRY
(2022)
Review
Chemistry, Physical
Francisco T. T. Cavalcante, Antonio L. G. Cavalcante, Isamayra G. de Sousa, Francisco S. Neto, Jose C. S. dos Santos
Summary: The market for industrial enzymes is growing rapidly, with lipases being the most widely used industrial biocatalysts. However, challenges such as low stability and complex solutions require new materials and technologies to improve their performance.
Article
Materials Science, Multidisciplinary
Antonio L. Medina-Castillo, Lucija Ruzic, Bernd Nidetzky, Juan M. Bolivar
Summary: High quality nonwoven nanofiber membranes with excellent mechanical and chemical properties were fabricated by electrospinning and used for enzyme immobilization. Two different polymer blends were successfully functionalized to immobilize two enzymes.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Applied
Lucas Dal Magro, Joao Pedro S. Pessoa, Manuela P. Klein, Roberto Fernandez-Lafuente, Rafael C. Rodrigues
Summary: This study immobilized a commercial enzyme cocktail for fruit juice clarification on chitosan beads and found that despite low activity recovery after immobilization, the stability of the immobilized enzyme was greater than that of the free enzyme. The enzyme retained higher activity levels under extreme conditions, and the fluidized-bed reactor showed better juice clarification capacity compared to the packed-bed reactor.
Article
Chemistry, Applied
Murilo Amaral-Fonseca, Roberto Morellon-Sterling, Roberto Fernandez-Lafuente, Paulo Waldir Tardioli
Summary: A new single-step saccharification and isomerization process using immobilized commercial enzymes has been proposed in this study, achieving high efficiency in high fructose syrup production by optimizing pH, temperature, and enzyme ratio. Experimental results show that this method not only improves yield, but also reduces catalyst loss, with good recyclability.
Article
Chemistry, Applied
Rodolpho R. C. Monteiro, Jose J. Virgen-Ortiz, Angel Berenguer-Murcia, Thays N. da Rocha, Jose C. S. dos Santos, Andres R. Alcantara, Roberto Fernandez-Lafuente
Summary: This review presents some of the latest studies on lipase A from Candida antarctica (CALA), focusing on its stability and ability to attack the sn-2 position of triglycerides, as well as its commercial availability. It discusses the cloning, production, structural facts, and applications of the enzyme, with a special emphasis on enzyme immobilization.
Article
Chemistry, Applied
Keysson V. Fernandes, Elisa D. C. Cavalcanti, Eliane P. Cipolatti, Erika C. G. Aguieiras, Martina C. C. Pinto, Fernanda A. Tavares, Priscila R. da Silva, Roberto Fernandez-Lafuente, Sara Arana-Pena, Jose Carlos Pinto, Charles L. B. Assuncao, Jose Andre C. da Silva, Denise M. G. Freire
Summary: This study successfully synthesized biolubricants from by-products of soybean oil processing using the lipase from Candida rugosa, showing high conversion rates and improved efficiency through immobilization. The products demonstrated good lubricant properties, indicating a potential environmentally friendly alternative to petroleum-based lubricants.
Article
Chemistry, Applied
Sara Arana-Pena, Nathalia S. Rios, Diego Carballares, Luciana R. B. Goncalves, Roberto Fernandez-Lafuente
Summary: CALB and CRL showed similar properties under different immobilization conditions, while RML displayed significant changes. The effects of immobilization conditions on substrate activity and stability vary greatly, requiring careful selection based on specific circumstances.
Review
Biotechnology & Applied Microbiology
Sara Arana-Pena, Diego Carballares, Roberto Morellon-Sterlling, Angel Berenguer-Murcia, Andres R. Alcantara, Rafael C. Rodrigues, Roberto Fernandez-Lafuente
Summary: Enzyme co-immobilization offers kinetic advantages but also drawbacks, such as the need to discard the entire biocatalyst if one enzyme becomes inactivated. The co-immobilization of cofactors and enzymes is of particular interest for the construction of artificial cells and complex one-pot transformations.
BIOTECHNOLOGY ADVANCES
(2021)
Review
Chemistry, Physical
Rodolpho R. C. Monteiro, Jose C. S. dos Santos, Andres R. Alcantara, Roberto Fernandez-Lafuente
Article
Biochemistry & Molecular Biology
Esteban Plata, Monica Ruiz, Jennifer Ruiz, Claudia Ortiz, John J. Castillo, Roberto Fernandez-Lafuente
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Physical
Sara Arana-Pena, Diego Carballares, Vicente Cortes Corberan, Roberto Fernandez-Lafuente
Article
Biochemistry & Molecular Biology
Jose Renato Guimaraes, Leticia Passos Miranda, Roberto Fernandez-Lafuente, Paulo Waldir Tardioli
Summary: The study evaluated the performance of Eversa-mCLEA in the enzymatic synthesis of biolubricants, showing good activity in transesterification reactions with different alcohols. By adjusting the WCO/alcohol molar ratio and enzyme load, high yields of bio-lubricants can be achieved.
Article
Biotechnology & Applied Microbiology
Dilek Alagoz, Ali Toprak, Deniz Yildirim, S. Seyhan Tukel, Roberto Fernandez- Lafuente
Summary: Different methods of immobilizing RML were studied, showing varying characteristics in activity, kinetic parameters, and recyclability. RML immobilized on Si-Glu exhibited higher catalytic efficiency and longer half-life.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sabrina Ait Braham, El Hocine Siar, Sara Arana-Pena, Hossein Bavandi, Diego Carballares, Roberto Morellon-Sterling, Diandra de Andrades, Jakub F. Kornecki, Roberto Fernandez-Lafuente
Summary: This study investigates the impact of glycerol on the stability of 13 immobilized enzymes, finding that its stabilizing effect is enzyme-dependent and influenced by concentration and pH. The results emphasize the importance of considering enzyme type, glycerol concentration, and pH level when using glycerol as a stabilizing agent.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Nicole S. Okura, Guilherme J. Sabi, Marcela C. Crivellenti, Raphael A. B. Gomes, Roberto Fernandez-Lafuente, Adriano A. Mendes
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Biochemistry & Molecular Biology
Roberto Morellon-Sterling, Hocine El-Siar, Olga L. Tavano, Angel Berenguer-Murcia, Roberto Fernandez-Lafuente
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Chemistry, Physical
Jakub F. Kornecki, Diego Carballares, Paulo W. Tardioli, Rafael C. Rodrigues, Angel Berenguer-Murcia, Andres R. Alcantara, Roberto Fernandez-Lafuente
CATALYSIS SCIENCE & TECHNOLOGY
(2020)