Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system

Electron cryo-microscopy structure of the mechanotransduction channel NOMPC

(2017) Peng Jin et al.   NATURE

Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3

(2017) Marscha Hirschi et al.   NATURE

The cryo-electron microscopy structure of human transcription factor IIH

(2017) Basil J. Greber et al.   NATURE

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Rigidly connected multispecific artificial binders with adjustable geometries

(2017) Yufan Wu et al.   Scientific Reports

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

DARPin-Based Crystallization Chaperones Exploit Molecular Geometry as a Screening Dimension in Protein Crystallography

(2016) Alexander Batyuk et al.   JOURNAL OF MOLECULAR BIOLOGY

Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools

(2016) Marco Schütz et al.   JOURNAL OF MOLECULAR BIOLOGY

TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action

(2016) Yuan Gao et al.   NATURE

How good can cryo-EM become?

(2016) Robert M Glaeser   NATURE METHODS

The development of cryo-EM into a mainstream structural biology technique

(2016) Eva Nogales   NATURE METHODS

Flexible, symmetry-directed approach to assembling protein cages

(2016) Aaron Sciore et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

2.3 A resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition

(2016) S. Banerjee et al.   SCIENCE

Accurate design of megadalton-scale two-component icosahedral protein complexes

(2016) J. B. Bale et al.   SCIENCE

Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta

(2016) Ray Yu-Ruei Wang et al.   eLife

Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein

(2016) Francesca Coscia et al.   Scientific Reports

Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy

(2015) Andreas Plückthun   Annual Review of Pharmacology and Toxicology

Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression

(2015) Jacob B. Bale et al.   PROTEIN SCIENCE

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

(2015) Melody G Campbell et al.   eLife

Accurate design of co-assembling multi-component protein nanomaterials

(2014) Neil P. King et al.   NATURE

Structure of a designed protein cage that self-assembles into a highly porous cube

(2014) Yen-Ting Lai et al.   Nature Chemistry

Re-engineering protein interfaces yields copper-inducible ferritin cage assembly

(2013) Dustin J E Huard et al.   Nature Chemical Biology

Quantifying the local resolution of cryo-EM density maps

(2013) Alp Kucukelbir et al.   NATURE METHODS

Design, construction, and characterization of a second-generation DARPin library with reduced hydrophobicity

(2013) Markus A. Seeger et al.   PROTEIN SCIENCE

Relaxation of backbone bond geometry improves protein energy landscape modeling

(2013) Patrick Conway et al.   PROTEIN SCIENCE

Self-Assembling Cages from Coiled-Coil Peptide Modules

(2013) J. M. Fletcher et al.   SCIENCE

Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries

(2012) L. Kummer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end

(2012) Ludovic Pecqueur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of a 16-nm Cage Designed by Using Protein Oligomers

(2012) Y.-T. Lai et al.   SCIENCE

Systematic control of protein interaction using a modular ER/K  -helix linker

(2011) S. Sivaramakrishnan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structural Determinants for Improved Stability of Designed Ankyrin Repeat Proteins with a Redesigned C-Capping Module

(2010) Michaela A. Kramer et al.   JOURNAL OF MOLECULAR BIOLOGY

Residue-Resolved Stability of Full-Consensus Ankyrin Repeat Proteins Probed by NMR

(2010) Svava K. Wetzel et al.   JOURNAL OF MOLECULAR BIOLOGY

Crystal Structure and Function of a DARPin Neutralizing Inhibitor of Lactococcal Phage TP901-1

(2009) David Veesler et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Light-activated DNA binding in a designed allosteric protein

(2008) D. Strickland et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic charge interactions create surprising rigidity in the ER/K  -helical protein motif

(2008) S. Sivaramakrishnan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chaperone-Assisted Crystallography with DARPins

(2008) Gaby Sennhauser et al.   STRUCTURE