Article
Biochemistry & Molecular Biology
Christian O. O. Martins, Leticia K. K. Sebastiany, Alejandro Lopez-Castillo, Rafael S. S. Freitas, Leandro H. H. Andrade, Henrique E. E. Toma, Caterina G. C. Marques Netto
Summary: Despite their similarity to the enzyme active site, inorganic models are unable to mimic the mechanism of urease. By synthesizing two nickel complexes, it was found that the formation of an outer-sphere complex in urease analogues may be the reason for the difference in mechanism.
Article
Chemistry, Inorganic & Nuclear
Bidyut Kumar Kundu, Pragti, Soumen Biswas, Abhijit Mondal, Shyamalava Mazumdar, Shaikh M. Mobin, Suman Mukhopadhyay
Summary: The study synthesized and characterized two dinickel(II) complexes which exhibited urease-like activity, liberating ammonia in aqueous solution. The liberated ammonia was utilized for the synthesis of biologically active products with high yields in aqueous medium.
DALTON TRANSACTIONS
(2021)
Review
Environmental Sciences
Marta Klimczyk, Anna Siczek, Lech Schimmelpfennig
Summary: The addition of urease inhibitor NBPT to urea fertilizers can reduce ammonia emissions, while nitrification inhibitors may lead to an increase in ammonia emission. Factors such as dose and date of application, method of application, type of soil and pH, as well as atmospheric conditions, also have an impact on ammonia emissions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Baha Ondes, Fatma Akpinar, Murat Uygun, Mihrican Muti, Deniz Aktas Uygun
Summary: A highly stable urea biosensor was prepared by immobilizing urease enzyme onto nanoparticles, showing a detection limit of 0.77 mu M and a linear concentration range of 0.01 mM-500 mM. The biosensor system exhibited high repeatability and retained a certain level of activity after long-term storage. Interference from common interferents was found to be negligible, and the biosensor was proven suitable for detecting urea in artificial human serum.
MICROCHEMICAL JOURNAL
(2021)
Article
Polymer Science
Natalia Guerrero-Alburquerque, Shanyu Zhao, Daniel Rentsch, Matthias M. Koebel, Marco Lattuada, Wim J. Malfait
Summary: Urea, as an alternative to KOCN, shows potential for grafting ureido groups onto amines in aqueous media due to its low cost, non-toxicity, and biocompatibility. Reaction times are almost independent of substrate and pH, but excess urea is required for practically feasible reaction rates.
Article
Engineering, Environmental
Salhah D. Al-Qahtani, Hanan K. Alzahrani, Omer A. Azher, Zeid O. Owidah, Matokah Abualnaja, Turki M. Habeebullah, Nashwa M. El-Metwaly
Summary: A high-sensitivity viscose fabric was developed for colorimetric recognition of urea in fluids using co-encapsulated calcium alginate nanoparticles. Extracted anthocyanin from red-cabbage and urease enzyme were studied for their role in the fabrication of a biosensor on fabrics. The colorimetric changes of the fabric were monitored with increasing urea concentration, showing a shift in absorbance spectra and color variations.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Analytical
Yuan Yuan, Yuhan He, Dongni Pei, Liujuan Tong, Shengqiang Hu, Lin Liu, Xinyao Yi, Jianxiu Wang
Summary: Urease-functionalized near-infrared (NIR) light-responsive gold nanoflowers (AuNFs) were used as a sensing platform for urea assay. By changing the temperature and activity of the urease-AuNFs composites, the concentration of urea could be rapidly detected. This method conveniently measured urea in blood samples within 10 minutes, and the results were in good agreement with clinical results.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Physical
Yue Shi, Huiru Li, Dana Ao, Ying Chang, Aiju Xu, Meilin Jia, Jingchun Jia
Summary: This study synthesized nickel selenide and 3D nickel diselenide architectures on N-doped carbon successfully, showing good catalytic activity for methanol oxidation and urea oxidation reactions. The enhanced performance of NiSe2/NC-450 is attributed to the expanded electrochemically active area after selenization and the enhanced conductivity after N-doped carbon.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Materials Science, Multidisciplinary
Reena K. Sajwan, Purnima Sharma, G. B. V. S. Lakshmi, Pratima R. Solanki
Summary: A fluorescence Turn-On based strategy using MPA-CdSe QDs was utilized for urea determination, with a detection range from 1 to 120 mM and a lower detection limit of 1 mM. The pH sensitivity of MPA-CdSe QDs was key in detecting urea through the enzymatic reaction with urease and the release of ammonia.
Article
Chemistry, Analytical
Fares Zouaoui, Nadia Zine, Abdelhamid Errachid, Nicole Jaffrezic-Renault
Summary: In this article, a mathematical model was developed to optimize the configuration of a urea biosensor. The model was validated using experimental data and can simulate the biosensor's response under different conditions, guiding experiments, improving analytical performance, and reducing development costs.
Article
Environmental Sciences
Rui Zhao, Juying Liu, Na Xu, Tianyi He, Jun Meng, Zunqi Liu
Summary: Biochar has different effects on urease activity and nitrogen content in different soil types. It enhances urease activity by stimulating urease-producing microorganisms and increasing soil pH. However, in some cases, biochar may lead to an increase in nitrogen loss.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Chemistry, Physical
Linlin Yang, Ren He, Xiang Wang, Tingting Yang, Ting Zhang, Yong Zuo, Xuan Lu, Zhifu Liang, Junshan Li, Jordi Arbiol, Paulina R. Martinez-Alanis, Xueqiang Qi, Andreu Cabot
Summary: Direct urea fuel cells (DUFCs) offer a solution for treating residual wastewater and generating electricity. The use of self-supported electrodes consisting of NiO nanosheets grown on CuO nanowires proves to be effective for urea oxidation reaction (UOR). These electrodes exhibit excellent UOR performance, requiring 1.39 V vs. RHE to achieve a current density of 100 mA/cm2. Additionally, the DUFCs demonstrate high open circuit voltages and power densities of 0.88 V and 11.35 mW/cm2, respectively. The formation of NiOOH and the presence of a NiO/CuO p-p heterostructure contribute to the enhanced performance through improved charge transfer and modulated electronic states.
Article
Oncology
Michael J. Maroney, Stefano Ciurli
Summary: Helicobacter pylori, a human pathogen causing peptic ulcers and classified as a Class I carcinogen, relies on nickel-urease and hydrogenase enzymes for survival and virulence. Nickel plays a crucial role in virulence, and proteins involved in nickel trafficking may be potential antibiotic targets.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Chemistry, Analytical
Hongqin Liu, Dongsheng Wen, Bingjun Zhu
Summary: Urea electrolysis is a promising method for treating urea-polluted water and producing hydrogen fuel as clean energy. In this study, a hierarchically three-dimensional structure is obtained by growing nickel sulfide composites on nickel foam, which shows efficient urea oxidation reaction catalytic performance and practical application in urine and urea-rich wastewater treatment.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Sivakumar Gowthaman, Moeka Yamamoto, Kazunori Nakashima, Volodymyr Ivanov, Satoru Kawasaki
Summary: Biocementation technology is a new soil improvement method which involves the enzymatic formation of calcium carbonate or calcium phosphate. Compared to conventional methods, calcium phosphate biocementation can control the release of ammonium ions and gaseous ammonia at different pH ranges, while improving the soil's compressive strength.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Agricultural Engineering
Luis R. A. Gabriel Filho, Alexsandro O. da Silva, Camila Pires Cremasco, Fernando Ferrari Putti
Summary: The objective of this study was to develop a Fuzzy Rule-Based System (FRBS) for mathematically modeling the effect of irrigation level on beet cultivars. The results showed that the fuzzy models performed better in predicting beet yield, root diameter, and plant height, and revealed that the Early Wonder cultivar has higher yields when subjected to water deficit.
ENGENHARIA AGRICOLA
(2022)
Article
Plant Sciences
Kamilla Silva Oliveira, Renato de Mello Prado, Mirela Vantini Checchio, Priscila Lupino Gratao
Summary: The results of this study suggest that silicon can alleviate the negative effects of manganese deficiency on energy cane, improving its antioxidant capacity and manganese uptake efficiency, and promoting growth.
Article
Chemistry, Physical
Rita de Cassia Alves, Maria Fernanda dos Santos Zucco, Kevein Ruas Oliveira, Mirela Vantini Checchio, Carolina Andrade Franco, Katalin Korosi, Priscila Lupino Gratao
Summary: The study demonstrated the potential of silicon (Si) in inducing resistance to lettuce downy mildew. The results showed that Si reduced disease incidence, increased photosynthetic pigments contents, maintained seedling growth, reduced harmful substances content, and increased the activity of antioxidant enzymes.
Article
Agronomy
Andre L. Abreu, Priscila L. Gratao, Carlos A. V. Rodriguez, Gilmar S. Sousa Junior
Summary: This study assesses the protective effect of a novel mineral composition, consisting of concentrated suspension of microparticles of crystalline and insoluble quartz sand, applied as foliar spray over plants to mitigate the stress effects of UV-A or UV-B radiation. The results demonstrate that the foliar spray effectively mitigates the damages caused by UV-A and UV-B radiation, and enhances the sustainable yield of soybeans and potentially other field crops. This study provides important insights for growers in facing the increasing challenges of climate change.
Article
Agronomy
Nandhara Angelica Carvalho Mendes, Maria Gabriela Dantas Bereta Lanza, Vinicius Martins Silva, Marco Antonio Bosse, Gabriel Sgarbiero Montanha, Hudson Wallace Pereira de Carvalho, Matheus Luis Oliveira Cunha, Andre Rodrigues dos Reis
Summary: Selenium has a narrow range between beneficial and toxic concentrations for plants, and dose-response studies are needed to determine critical doses of Se toxicity. This study characterized the dose response of Se in cowpea plants by evaluating ureide metabolism, ultrastructural responses, and Se distribution. Results showed that Se concentration in leaves and seeds increased linearly with sodium selenite application. Se application also increased ureide and nitrate concentrations in cowpea leaves, and the increase in ureides was attributed to biologically fixed nitrogen and antioxidant capacity. However, above a certain dose, Se induced chlorosis and necrotic lesions on the leaf blade. X-ray fluorescence spectroscopy confirmed higher Se count rates in the necrotic regions, indicating the toxicity of Se. Calcium intensity also increased in regions with higher Se intensity, suggesting a possible role of Ca in attenuating Se stress. Selenium was found throughout all seed regions, especially in the embryo.
Article
Chemistry, Analytical
Yonatan Meir, Jayme Garcia Arnal Barbedo, Omri Keren, Claudia Vieira Godoy, Nofar Amedi, Yaar Shalom, Amir B. Geva
Summary: One of the biggest challenges in applying computer vision and artificial intelligence to agriculture is the time-consuming and expensive process of labeling training data for models. This study explores using electroencephalograms from plant pathology experts to improve the accuracy and robustness of image-based AI models for plant disease recognition. The experiments demonstrate that this approach can significantly reduce labeling time and improve accuracies from 96% to 99% using brain-generated labels and active learning.
Article
Computer Science, Interdisciplinary Applications
Samuel da Costa Alves Basilio, Fernando Ferrari Putti, Angelica Carvalho Cunha, Leonardo Goliatt
Summary: Solar radiation prediction is necessary for various applications. This study presents a methodology that optimizes machine learning models to efficiently predict solar radiation in Minas Gerais, Brazil. The results show that the computational models perform better than the empirical models currently used.
EARTH SCIENCE INFORMATICS
(2023)
Article
Horticulture
Rafaelly Calsavara Martins, Sarita Leonel, Jackson Mirellys Azevedo Souza, Giuseppina Pace Pereira Lima, Magali Leonel, Fernando Ferrari Putti, Gean Charles Monteiro, Patricia Graosque uelguim Zuge, Gabriel Maluf Napoleao, Ricardo Figueira, Jaime Duarte Filho
Summary: The concentrations of bioactive compounds in orange juice are affected by different citrus scion/rootstock combinations. Packaging and storage can optimize the shelf life of freshly squeezed juice. The combination of 'Rubi' orange in both 'Rangpur' lime and 'Swingle' citrumelo rootstocks showed higher concentrations of ascorbic acid and dehydroascorbic acid. Overall, scions grafted onto 'Swingle' citrumelo rootstock had higher concentrations of ascorbic acid, dehydroascorbic acid, and some phenolic compounds. Phenolic compounds had the highest concentrations at the time of fruit extraction and decreased during storage. Dark packaging increased the levels of bioactive compounds in juices stored for longer periods. These findings can help diversify scion/rootstock cultivars to produce pasteurized orange juice with higher nutritional value.
Article
Horticulture
Fernando Ferrari Putti, Eduardo Festozo Vicente, Prinscilla Pamela Nunes Chaves, Luis Paulo Benetti Mantoan, Camila Pires Cremasco, Bruna Arruda, Juliane Cristina Forti, Josue Ferreira Silva, Marcelo Campos, Andre Rodrigues dos Reis, Luis Roberto Almeida Gabriel
Summary: Climate change is affecting vegetable production through changes in rainfall patterns and soil moisture. To mitigate this, technologies such as magnetically treated water are being explored to maintain ideal soil moisture for plant uptake. This study investigated the effect of magnetically treated water on lettuce biomass and nutrient uptake, finding that it improved nitrogen assimilation and resulted in higher agronomical characteristics and yield.
Article
Horticulture
Francisco Jose Domingues Neto, Adilson Pimentel Jr, Lenon Romano Modesto, Mara Fernandes Moura, Fernando Ferrari Putti, Carmen Silvia Fernandes Boaro, Elizabeth Orika Ono, Joao Domingos Rodrigues, Marco Antonio Tecchio
Summary: The study aimed to evaluate the influence of rootstock and trellis height on photosynthesis, biochemical, and yield performance of grapevine hybrids. The results showed that under subtropical conditions, better photosynthesis, biochemical, and yield performance were observed when both cultivars were grafted on the 'IAC 766' rootstock. The 'IAC 138-22 Maximo' trained until 2.0 and grafted on the 'IAC 766' rootstock, increasing grape production and photosynthesis efficiency.
Article
Plant Sciences
Gustavo Ferreira da Silva, Juliano Carlos Calonego, Bruno Cesar Ottoboni Luperini, Vinicius Brasil Silveira, Larissa Chamma, Rogerio Peres Soratto, Fernando Ferrari Putti
Summary: Soil management systems can directly impact crop yield by altering the physical and hydraulic properties of the soil. Long-term experiments have shown that the no-tillage system improves soil density and compaction resistance, enhances root development, and increases soybean yield by 6.5%, without affecting water infiltration.
Article
Plant Sciences
Fernando Ferrari Putti, Jessica Pigatto de Queiroz Barcelos, Bruno Cesar Goes, Renata Fernandes Alves, Mario Mollo Neto, Alexsandro Oliveira da Silva, Luis Roberto Almeida Gabriel Filho, Willian Aparecido Leotti Zanetti, Angela Vacaro de Souza
Summary: The objective of this study was to investigate the effects of very low-frequency electromagnetic resonance field treatment on water for tomato crops. The results showed that irrigation with treated water can reduce oxidative stress caused by water deficit and increase tomato crop productivity.
Article
Agriculture, Multidisciplinary
Rafaelly C. Martins, Sarita Leonel, Jackson M. A. Souza, Magali Leonel, Fernando F. Putti, Patricia G. U. Zuge, Rafael B. Ferreira, Marcelo S. Silva, Jaime Duarte Filho
Summary: The aim of this study was to investigate the diversification of citrus cultivation to increase variety and profitability of orchards in response to environmental changes. The study was conducted in the subtropical region of Sao Paulo, Brazil. The results showed that the rootstocks had little influence on the duration of phenological intervals. Scion cultivars grafted onto RL demonstrated larger canopy volumes and better fruit quality compared to those grafted onto SC.
SPANISH JOURNAL OF AGRICULTURAL RESEARCH
(2023)
Article
Biology
Gustavo Ferreira da Silva, Nilbe Carla Mapeli, Cassiano Cremon, Larissa Chamma, Gabrieli Ferreira da Silva, Bruno Cesar Ottoboni Luperini, Fernando Ferrari Putti
Summary: This study analyzed the impact of different diluted solutions applied to soybean plants and consumed by Anticarsia gemmatalis on insect biology. The results showed that the use of diluted solutions is an effective alternative method for controlling A. gemmatalis, reducing its food conversion efficiency and biological benefits.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2023)
Article
Plant Sciences
Fernando Ferrari Putti, Camila Pires Cremasco, Alfredo Bonini Neto, Ana Carolina Kummer Barbosa, Josue Ferreira da Silva Junior, Andre Rodrigues dos Reis, Bruno Cesar Goes, Bruna Arruda, Luis Roberto Almeida Gabriel Filho
Summary: This study used a mathematical model based on fuzzy logic to estimate the replacement rates of magnetic water provided by irrigation and compared it with multiple polynomial regression analysis. The results showed that irrigating with magnetic water resulted in higher biometric values for lettuce, and the fuzzy model provided a more accurate adjustment compared to multiple polynomial regression.