Efficient in planta gene targeting in Arabidopsis using egg cell-specific expression of the Cas9 nuclease of Staphylococcus aureus

Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation

(2018) Ahmed Mahas et al.   BIOTECHNOLOGY ADVANCES

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

(2017) Mugui Wang et al.   Molecular Plant

Erratum: Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2017) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Towards CRISPR/Cas crops - bringing together genomics and genome editing

(2017) Armin Scheben et al.   NEW PHYTOLOGIST

High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9

(2017) Javier Gil-Humanes et al.   PLANT JOURNAL

From classical mutagenesis to nuclease-based breeding - directing natural DNA repair for a natural end-product

(2017) Michael Pacher et al.   PLANT JOURNAL

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks

(2017) Alexandre Paix et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus

(2017) Anne Bothmer et al.   Nature Communications

Plant genome editing with TALEN and CRISPR

(2017) Aimee Malzahn et al.   Cell and Bioscience

Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule

(2017) Haroon Butt et al.   Frontiers in Plant Science

Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases

(2017) Matthew B. Begemann et al.   Scientific Reports

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2016) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

(2016) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks

(2016) Lianne E.M. Vriend et al.   NUCLEIC ACIDS RESEARCH

Homology-based double-strand break-induced genome engineering in plants

(2016) Jeannette Steinert et al.   PLANT CELL REPORTS

Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes

(2016) Holger Puchta   PLANT JOURNAL

Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas

(2016) Simon Schiml et al.   Plant Methods

Oligonucleotide-Mediated Genome Editing Provides Precision and Function to Engineered Nucleases and Antibiotics in Plants

(2016) Noel J. Sauer et al.   PLANT PHYSIOLOGY

Repair of adjacent single-strand breaks is often accompanied by the formation of tandem sequence duplications in plant genomes

(2016) Simon Schiml et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Biochemical mechanism of DSB end resection and its regulation

(2015) James M. Daley et al.   DNA REPAIR

Stable gene replacement in barley by targeted double-strand break induction

(2015) Koichi Watanabe et al.   JOURNAL OF EXPERIMENTAL BOTANY

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System

(2015) Liuhua Yan et al.   Molecular Plant

Highly efficient heritable plant genome engineering using Cas9 orthologues fromStreptococcus thermophilusandStaphylococcus aureus

(2015) Jeannette Steinert et al.   PLANT JOURNAL

Biallelic Gene Targeting in Rice

(2015) Masaki Endo et al.   PLANT PHYSIOLOGY

A Defect in DNA Ligase4 Enhances the Frequency of TALEN-Mediated Targeted Mutagenesis in Rice

(2015) Ayako Nishizawa-Yokoi et al.   PLANT PHYSIOLOGY

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

(2015) Sergei Svitashev et al.   PLANT PHYSIOLOGY

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

(2015) Zhi-Ping Wang et al.   GENOME BIOLOGY

DNA recombination in somatic plant cells: mechanisms and evolutionary consequences

(2014) Alexander Knoll et al.   CHROMOSOME RESEARCH

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

The CRISPR/Cas system can be used as nuclease forin plantagene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

(2014) Simon Schiml et al.   PLANT JOURNAL

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering inArabidopsis thaliana

(2014) Friedrich Fauser et al.   PLANT JOURNAL

Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair

(2014) L. Davis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gene targeting in plants: 25 years later

(2013) Holger Puchta et al.   INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

Gene targeting in maize by somatic ectopic recombination

(2012) Ayhan Ayar et al.   PLANT BIOTECHNOLOGY JOURNAL

ZFN-mediated gene targeting of the Arabidopsisprotoporphyrinogen oxidasegene throughAgrobacterium-mediated floral dip transformation

(2012) Sylvia de Pater et al.   PLANT BIOTECHNOLOGY JOURNAL

In Planta Somatic Homologous Recombination Assay Revisited: A Successful and Versatile, but Delicate Tool

(2012) H. Puchta et al.   PLANT CELL

The requirement for recombination factors differs considerably between different pathways of homologous double-strand break repair in somatic plant cells

(2012) Nadine Roth et al.   PLANT JOURNAL

In planta gene targeting

(2012) F. Fauser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Egg Cell-Secreted EC1 Triggers Sperm Cell Activation During Double Fertilization

(2012) S. Sprunck et al.   SCIENCE

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

Precise genome modification in the crop species Zea mays using zinc-finger nucleases

(2009) Vipula K. Shukla et al.   NATURE

Evaluation of seven promoters to achieve germline directed Cre-lox recombination in Arabidopsis thaliana

(2009) Frédéric Van Ex et al.   PLANT CELL REPORTS