Article
Microbiology
Martha V. Fernandez-Baca, Cristian Hoban, Rodrigo A. Ore, Pedro Ortiz, Young-Jun Choi, Cesar Murga-Moreno, Makedonka Mitreva, Miguel M. Cabada
Summary: This study characterized a large sample of Fasciola hepatica with varying triclabendazole susceptibility to determine the complexity of resistance in this parasite. The results showed that resistance varied by geographic location, season, and morphology, providing a basis for further research on the association between resistance phenotype and genotype.
Article
Agriculture, Dairy & Animal Science
Daniel Rondelaud, Philippe Vignoles, Gilles Dreyfuss
Summary: The study investigates the impact of global warming and drug treatment on snail populations and parasite infections. The findings reveal a decline in snail populations and a decrease in infection rates of one parasite but an increase in infections of another parasite. These changes are attributed to the widespread use of drugs and recent heatwave events.
Article
Infectious Diseases
Melinda Barbara Tanabe, John Prochaska, Maria Luisa Morales, Martha Lopez, Benicia Baca-Turpo, Eulogia Arque, Miguel Mauricio Cabada
Summary: Fasciola hepatica is a neglected parasitic infection that has a significant impact on human health and the livestock industry. This study aimed to investigate the geospatial relationships between Fasciola eggs in livestock feces and the risk of infection in households. The results showed that infected and uninfected cattle and sheep feces were more likely to be found near households with infected children compared to households without infected children. The study also identified areas with higher and lower infection rates. This information and strategy can be used for the control of the disease.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
Santiago Mas-Coma, Maria Mercedes Cafrune, Ilra Renata Funatsu, Atilio Jose Mangold, Rene Angles, Paola Buchon, Maria Cecilia Fantozzi, Patricio Artigas, Maria Adela Valero, Maria Dolores Bargues
Summary: Although South American camelids serve as definitive hosts for Fasciola hepatica, their role in transmission and epidemiology of the disease has not been thoroughly studied. Experimental investigations suggest that the contribution of llamas to fascioliasis transmission is negligible, and therefore, they do not need to receive priority in control measures in endemic areas.
Article
Parasitology
Tania Rojas-Campos, Froylan Ibarra-Velarde, Yolanda Vera-Montenegro, Miguel Flores-Ramos, Irene Cruz-Mendoza, Gerardo Leyva-Gomez, Alicia Hernandez-Campos
Summary: This study compared the fasciolicidal efficacy of a new injectable formulation of fosfatriclaben with subcutaneous closantel and oral triclabendazole. Results showed that fosfatriclaben and triclabendazole had similar efficacy, but fosfatriclaben's intramuscular administration was more convenient and had lower dosage.
VETERINARY PARASITOLOGY
(2021)
Article
Parasitology
Carolin M. Morawietz, Alejandra M. Peter Ventura, Christoph G. Grevelding, Simone Haeberlein, Bernhard Spengler
Summary: Understanding drug penetration, distribution, and metabolization in parasites is essential for drug efficacy. Recently, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has been used to visualize the distribution patterns of drug compounds in blood flukes and liver flukes. This technique provides high spatial resolution and has the potential to contribute to preclinical drug discovery in liver fluke research.
PARASITOLOGY RESEARCH
(2022)
Article
Parasitology
Froylan Ibarra-Velarde, Tania Rojas-Campos, Yolanda Vera-Montenegro, Miguel Flores-Ramos, Rafael Castillo, Gerardo Leyva-Gomez
Summary: The study aimed to determine the effective dose of fosfatriclaben through a field study in sheep naturally infected with F. hepatica. Results showed that fosfatriclaben had a high fasciolicidal efficacy similar to triclabendazole, with only half the dose required to remove eggs and flukes in the sheep studied.
PARASITOLOGY RESEARCH
(2022)
Article
Veterinary Sciences
Jane M. Kelley, Grant Rawlin, Travis Beddoe, Mark Stevenson, Terry W. Spithill
Summary: This study in Australia highlighted deficiencies in controlling Fasciola hepatica infection in dairy cattle, such as excessive use of clorsulon, limited rotation of flukicides, lack of diagnostic testing to inform treatment, and potential underdosing of animals. Communication gaps were identified in diagnostic testing for flukicide use, rotation of flukicide actives, flukicide administration, and testing of replacement animals.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Parasitology
Anand Chakroborty, Deiniol Pritchard, Marc E. Bouillon, Anna Cervi, Alan Cookson, Charlotte Wild, Caroline Fenn, Joseph Payne, Peter Holdsworth, Colin Capner, Jenna O'Neill, Gilda Padalino, Josephine Forde-Thomas, Sandeep Gupta, Brendan G. Smith, Maggie Fisher, Martina Lahmann, Mark S. Baird, Karl F. Hoffmann
Summary: Control of liver fluke infections is a significant challenge in the livestock sector due to drug resistant parasites. This study evaluated the anthelmintic activities of chemically modified abietic acid analogues against F. hepatica and found moderate activity in one analogue, but it also showed toxicity to mammalian cells. Therefore, further medicinal chemistry improvements are needed to consider these analogues as potential anthelmintics against liver fluke pathogens.
VETERINARY PARASITOLOGY
(2022)
Review
Infectious Diseases
Mpumelelo Ian Hadebe, Tawanda Manyangadze, Chester Kalinda, Tafadzwa Mindu, Moses John Chimbari
Summary: This systematic review and meta-analysis examined the infection rates and distribution of Fasciola spp. in intermediate host snails in Africa. The study found that Galba truncatula had an overall infectivity prevalence of 52%, while Radix natalensis and Pseudosuccinea columella had infectivity prevalences of 8% and 3% respectively. Native snail species in Africa (R. natalensis and G. truncatula) had been studied more extensively than the invasive P. columella. The highest prevalence of G. truncatula was found in North Africa, with an infection rate of 52%.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Angelica Terashima, Marco Canales, Vicente Maco, A. Luis Marcos
Summary: This retrospective study in Peru investigated alternative TCBZ regimens for patients with chronic fascioliasis who failed the standard-of-care treatment, showing a cure rate of 74%. The term 'TCBZ resistance' may need to be re-evaluated using biomarkers to guide treatment decisions.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Claud Roldan, Mattia Begovoeva, Jorge Ramon Lopez-Olvera, Roser Velarde, Oscar Cabezon, Anna Rita Molinar Min, Federica Pizzato, Mario Pasquetti, Xavier Fernandez Aguilar, Gregorio Mentaberre, Emmanuel Serrano, Maria Puig Ribas, Johan Espunyes, Raquel Castillo-Contreras, Josep Estruch, Luca Rossi
Summary: This study investigated the presence of Fasciola hepatica in a multi-host system in a European alpine area. The liver parasite was detected in cattle, sheep, chamois, and domestic cattle, with a higher prevalence in the former two species. Hepatic lesions associated with F. hepatica were observed in some chamois. The DNA of the parasite was found in a very low percentage of freshwater snails in the area.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Parasitology
Monique Costa, Anderson Saravia, Diego Ubios, Pablo Lores, Valeria da Costa, Maria Florencia Festari, Mercedes Landeira, Santiago A. Rodriguez-Zraquia, Georgget Banchero, Teresa Freire
Summary: The aim of this research was to investigate the relationship between hepatic function, hematological parameters, leukocyte counts in circulation, parasite egg shedding, and the effects of triclabendazole (TCZ) treatment during Fasciola hepatica infection in cattle. The results showed increased levels of serum aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) in the early stages of infection. Additionally, high circulating eosinophil count and plateletcrit levels were correlated with the number of flukes in the livers of infected cattle. TCZ treatment reduced parasite burden and liver damage in chronically infected cattle but did not completely eliminate them.
EXPERIMENTAL PARASITOLOGY
(2022)
Article
Multidisciplinary Sciences
Mohamed M. Salim, Sally Ashraf, Heba M. Hashem, Fathalla Belal
Summary: A simple and sensitive voltammetric method for the recognition of nitroxynil was developed and validated. The method utilized square wave voltammetry and showed linear relationship between the anodic peak current and nitroxynil concentration. The method was successfully applied to evaluate the drug in commercial vials and monitor residual amounts in various samples.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Michal Babjak, Alzbeta Konigova, L'udmila Burcakova, Michaela Komaromyova, Michaela Urda Dolinska, Marian Varady
Summary: This study compared in vivo and in vitro methods for detecting anthelmintic resistance and drug efficacy in a cattle farm with a long-term use of albendazole and a history of fasciolosis. Results showed that the in vivo and in vitro tests for F. hepatica were consistent, suggesting potential reduced efficacy of ABZ in the 12-hour version of the EHT.
VETERINARY SCIENCES
(2021)
Article
Parasitology
N. J. Beesley, K. Cwiklinski, D. J. L. Williams, J. Hodgkinson
Article
Parasitology
Nicola J. Beesley, Diana J. L. Williams, Steve Paterson, Jane Hodgkinson
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2017)
Article
Infectious Diseases
N. J. Beesley, C. Caminade, J. Charlier, R. J. Flynn, J. E. Hodgkinson, A. Martinez-Moreno, M. Martinez-Valladares, J. Perez, L. Rinaldi, D. J. L. Williams
TRANSBOUNDARY AND EMERGING DISEASES
(2018)
Article
Parasitology
J. Hodgkinson, K. Cwiklinski, N. J. Beesley, S. Paterson, D. J. L. Williams
Article
Parasitology
Nao Takeuchi-Storm, Matthew Denwood, Heidi Huus Petersen, Heidi Larsen Enemark, Anna-Sofie Stensgaard, Mita Eva Sengupta, Nicola Jane Beesley, Jane Hodgkinson, Diana Williams, Stig Milan Thamsborg
PARASITES & VECTORS
(2018)
Article
Veterinary Sciences
Juriah Kamaludeen, John Graham-Brown, Nathalie Stephens, Josephine Miller, Alison Howell, Nicola J. Beesley, Jane Hodgkinson, Jane Learmount, Diana Williams
Article
Parasitology
Jane E. Hodgkinson, Ray M. Kaplan, Fiona Kenyon, Eric R. Morgan, Andrew W. Park, Steve Paterson, Simon A. Babayan, Nicola J. Beesley, Collette Britton, Umer Chaudhry, Stephen R. Doyle, Vanessa O. Ezenwa, Andy Fenton, Sue B. Howell, Roz Laing, Barbara K. Mable, Louise Matthews, Jennifer McIntyre, Catherine E. Milne, Thomas A. Morrison, Jamie C. Prentice, Neil D. Sargison, Diana J. L. Williams, Adrian J. Wolstenholme, Eileen Devaney
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2019)
Article
Parasitology
Nicola J. Beesley, Elizabeth Attree, Severo Vazquez-Prieto, Roman Vilas, Esperanza Paniagua, Florencio M. Ubeira, Oscar Jensen, Cesar Pruzzo, Jose D. Alvarez, Jorge Bruno Malandrini, Hugo Solana, Jane E. Hodgkinson
Summary: Population genetic studies on liver fluke populations in four provinces of Argentina revealed significant genetic structuring, with three population clusters identified. Despite the population clustering, there was no deviation from Hardy-Weinberg equilibrium, suggesting random mating within sub-populations. A high clonal diversity of 82% was observed, with 26.6% of parasites displaying identical genotypes, indicating potential clonemate transmission.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Microbiology
Nicola J. Beesley, Krystyna Cwiklinski, Katherine E. Allen, Rebecca C. Hoyle, Terry W. G. Spithill, E. James La Course, Diana J. L. G. Williams, Steve Paterson, Jane E. G. Hodgkinson
Summary: Fasciola hepatica infection causes significant economic losses in livestock and poses a threat to human health. Triclabendazole (TCBZ) is the main drug used to control the infection, but resistance to TCBZ is a global problem. This study used genetic cross and whole-genome sequencing to identify genes associated with TCBZ resistance in F. hepatica, providing important insights for future anthelmintic treatments.