Article
Biochemistry & Molecular Biology
Cesar I. Ortiz-Garcia, Monica L. Salgado-Lucio, Ana L. Roa-Espitia, Aide A. Munoz-Sanchez, Joaquin Cordero-Martinez, Enrique O. Hernandez-Gonzalez
Summary: This study found that guinea pig and mouse spermatozoa express NOX2 and NOX4 during capacitation, and these enzymes play an important role in ROS production. The study also discovered an interaction between NOX2 and NOX4, which depends on the activation of calpain. The results indicate that NOX2 and NOX4 are the most important ROS producers during guinea pig and mouse sperm capacitation, and their activation depends on calpain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
David Weissman, Christoph Maack
Summary: Heart failure is a growing health burden globally, characterized by alterations in excitation-contraction coupling, cardiac energy deficit, and oxidative stress. While targeting metabolism may provide prognostic benefits, treatments specifically targeting reactive oxygen species (ROS) are still in preclinical stages. More research is needed in this area to develop effective therapies for heart failure.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Valentin Platel, Diane Lechevalier, Clara Bourreau, Sarah Renault, Ivana Soborova, Caroline Jeanniere, Ludovic Martin, Olivier Herault, Isabelle Corre, Nicolas Clere
Summary: The tumor microenvironment is important for the progression of melanoma, and recent studies have found that endothelial cells (EC) play a role in the endothelial-to-mesenchymal transition (EndMT). Reactive oxygen species (ROS), primarily produced by NAPDH oxidases (NOX), can control tumor growth. This study aimed to investigate the role of NOX1 and NOX2 in EndMT induced by conditioned media (CM) from melanoma cells. The results showed that NOX1 and NOX2 played important roles in EndMT.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Gastroenterology & Hepatology
Juan F. Burgueno, Julia Fritsch, Eddy E. Gonzalez, Kevin S. Landau, Ana M. Santander, Irina Fernandez, Hajar Hazime, Julie M. Davies, Rebeca Santaolalla, Matthew C. Phillips, Sophia Diaz, Rishu Dheer, Nivis Brito, Judith Pignac-Kobinger, Ester Fernandez, Gregory E. Conner, Maria T. Abreu
Summary: Chronic colonic inflammation can lead to dysplasia and cancer. Activation of TLR4 is associated with up-regulation of DUOX2 and NOX1, leading to increased epithelial production of H2O2, promoting colorectal tumor formation.
Article
Cell Biology
Marc Herb, Alexander Gluschko, Michael Schramm
Summary: Reactive oxygen species (ROS) play important roles in cellular processes but can also lead to diseases, their effects depending on cell type and ROS production conditions. Cellular redox balance, like cell pH and calcium levels, is tightly controlled, with evidence showing that cells regulate ROS production by controlling activation and localization factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Se-Hyun Oh, Ji-Sun Ahn, Eun-Joo Oh, You-Jin Kim, Ju-Min Yook, Jeong-Hoon Lim, Hee-Yeon Jung, Ji-Young Choi, Chan-Duck Kim, Sun-Hee Park, Yong-Lim Kim, Jang-Hee Cho
Summary: The lethal intraperitoneal injection dose of ML171 for both males and females is 500 mg/kg. No mortality was observed for lower doses of ML171. The safe dose of single intraperitoneal ML171 in ICR mice was 250 mg/kg or less. Further studies are needed to confirm the safety of ML171 in the human body.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Ana Karina Aranda-Rivera, Alfredo Cruz-Gregorio, Omar Emiliano Aparicio-Trejo, Ariadna Jazmin Ortega-Lozano, Jose Pedraza-Chaverri
Summary: In the UUO model, overproduction of ROS leads to alterations in signaling pathways, promoting inflammation, oxidative stress, and apoptosis, contributing to fibrosis development. Impairment of mitochondrial metabolism is also involved in this process.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Francesca Beretti, Francesca Farnetani, Luca Reggiani Bonetti, Luca Fabbiani, Manuela Zavatti, Antonino Maiorana, Giovanni Pellacani, Tullia Maraldi
Summary: This study reveals a correlation between high positivity for Nox4 and c-met in BRAF mutated patients with melanoma, and metastasis occurring at least one year later than diagnosis. In vitro experiments demonstrate that the HGF/c-met/Nox4/ROS axis triggers epithelial-mesenchymal transition. The findings suggest that Nox4 inhibitors could be a potential addition to current therapies for treating melanoma patients with BRAF mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Markus Waldeck-Weiermair, Shambhu Yadav, Jonas Kaynert, Venkata Revanth Thulabandu, Arvind K. Pandey, Fotios Spyropoulos, Taylor Covington, Apabrita Ayan Das, Christina Kruger, Thomas Michel
Summary: Statins have multiple protective effects on the cardiovascular system, including lowering cholesterol levels and antioxidant effects. Simvastatin, a statin drug, inhibits the production of oxidant H2O2 induced by histamine and vascular endothelial growth factor, and also regulates the localization of signaling protein Rac1.
Review
Biochemistry & Molecular Biology
Marc Herb, Michael Schramm
Summary: Reactive oxygen species (ROS) play essential roles in various cellular processes, including antimicrobial activity and immune signaling regulation. However, in-depth studies on ROS and their regulated target molecules are still limited. This review provides an overview of ROS sources, functions, and related probes, scavengers, and inhibitors.
Article
Ecology
Xiao-Zhang Yu, Yun-Peng Chu, Hua Zhang, Yu-Juan Lin, Peng Tian
Summary: This study showed that NOXs activity in rice was enhanced during SCN(-) exposure, but repressed by JA and NaHS. SCN(-) exposure increased the levels of reactive oxygen species significantly in rice tissues, but JA and NaHS application reduced these levels. The different expression patterns of NOX genes in rice roots and shoots suggested tissue-specific regulation and determination of NOX activity.
Article
Cardiac & Cardiovascular Systems
Sofiane Ouerd, Noureddine Idris-Khodja, Michelle Trindade, Nathanne S. Ferreira, Olga Berillo, Suellen C. Coelho, Mario F. Neves, Karin A. Jandeleit-Dahm, Pierre Paradis, Ernesto L. Schiffrin
Summary: Endothelial ET-1 overexpression exacerbates atherosclerosis, perivascular oxidative stress, and inflammation in type 1 diabetes through NOX1.
CARDIOVASCULAR RESEARCH
(2021)
Article
Multidisciplinary Sciences
Ichiro Matsuo, Naoya Kawamura, Yoshiki Ohnuki, Kenji Suita, Misao Ishikawa, Takehiro Matsubara, Yasumasa Mototani, Aiko Ito, Yoshio Hayakawa, Megumi Nariyama, Akinaka Morii, Kenichi Kiyomoto, Michinori Tsunoda, Kazuhiro Gomi, Satoshi Okumura
Summary: Oral infections, particularly periodontitis, have been linked to cardiovascular diseases, but the molecular mechanisms are not yet fully understood. This study injected lipopolysaccharide derived from Porphyromonas gingivalis (PG-LPS) into mice and found that it had negative effects on cardiac function. Further investigation revealed that the activation of TLR4-NOX4 signaling was involved in this impact.
Review
Biochemistry & Molecular Biology
Anthony L. Sylvester, David X. Zhang, Sophia Ran, Natalya S. Zinkevich
Summary: Reactive oxygen species (ROS) play important roles in health and disease, with both beneficial and harmful effects. Different sources of ROS have different roles in various pathologies. NOX1 is associated with hypertension through ROS production, making it a promising therapeutic target. NOX2 and NOX4 produce hydrogen peroxide, influencing cellular processes. Increased ROS production from NOX5 contributes to atherosclerosis.
Article
Biochemistry & Molecular Biology
Rami S. Najjar, Shengyu Mu, Rafaela G. Feresin
Summary: Blueberry polyphenols demonstrate anti-hypertensive properties by reducing oxidative stress and increasing nitric oxide levels.