Article
Chemistry, Analytical
Eduardo Sommella, Albino Carrizzo, Fabrizio Merciai, Veronica Di Sarno, Daniela Carbone, Massimiliano De Lucia, Simona Musella, Carmine Vecchione, Pietro Campiglia
Summary: This study investigated the potential effects of Spirulina platensis peptide SP6 on metabolic modulation in a high fat diet ApoE(-/-) mice atherosclerotic model. The results showed that SP6 peptide significantly decreased the levels of certain metabolites, including sphingolipids, glycerophospholipids, TCA cycle intermediates, and amino acids, which are key players in the atherosclerotic process and progression. The study suggests the potential of SP6 peptide in regulating atherosclerotic disease development and progression and demonstrates the utility of DI-FT-ICR as a fast analytical tool for metabolic changes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Ting Liu, Shu Lin
Summary: A rapid and sensitive method combining UHPLC-FT-ICR-MS was used to identify the chemical constituents in Lanqin oral liquid. A total of 441 compounds including alkaloids, flavonoids, terpenoids, organic acids, phenylpropanoids, and other types were identified or tentatively characterized. This study provides reference for quality control, higher quality standards, pharmacodynamic research, and understanding the relationship between constituents and therapeutic effects of Lanqin oral liquid.
Article
Energy & Fuels
Jianxun Wu, Weilai Zhang, Yuanfeng Wang, Yahe Zhang, Quan Shi
Summary: A series of novel carotenoid-derived non-thiadiamondoids cage organic sulfur compounds (OSCs) were identified from crude oil. Non-polar sulfur compounds were isolated using the methylation/demethylation method and characterized using FT-ICR MS and GC-MS. The cage OSCs were tentatively identified as 2-thiatricyclo[4.4.0.0<3.8>]decanes. These novel OSCs were found to be generated by biological sulfate reduction, providing new clues for the preservation of hydrocarbons in geological environments through sulfurization. The cage OSCs have the potential to be biomarkers for studying sedimentary environments.
Article
Biochemical Research Methods
Ting Liu, Ruiyun Li, Nan Zhang, Yue Cui, Yunli Zhao, Zhiguo Yu
Summary: UHPLC combined with Fourier-transform ion cyclotron resonance MS was used to identify differential components in the plasma of normal and pyrexia rats after oral administration of Gegenqinlian decoction. A validated UHPLC-MS method was developed for the simultaneous determination of these components. Pharmacokinetic study showed changes in the behavior of analytes in pyrexia rats compared to normal rats.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Engineering, Environmental
Dennys Leyva, Muhammad Usman Tariq, Rudolf Jaffe, Fahad Saeed, Francisco Fernandez-Lima
Summary: Dissolved Organic Matter (DOM) is a significant component of the global carbon cycle. This study used high-resolution mobility and ultrahigh-resolution Fourier transform ion cyclotron resonance tandem mass spectrometry (FT-ICR MS/MS) to compare four solid phase extracted (SPE) DOM samples from three freshwater ecosystems. Comparison of structural families revealed dissimilarities in the structural footprint of the samples. Inclusion of isomeric content in the structural family description suggested additional transformation pathways and/or source variations.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Energy & Fuels
Shunmugiah Mahendran, Subbiah Sankaralingam, Pandiaraj Maheswari, Pandian Annalakshmi, Jeyaraj Pandiarajan, Parthasarathy Seethapathy, Balasundaram Harinathan, Selvam Palpperumal, Natarajan Yasothkumar, Sakthivel Venkatesh
Summary: This study investigated the anticancer and antioxidant properties of phycocyanin isolated from Spirulina. The results showed that phycocyanin exhibited effective scavenging role and displayed anticancer activity against HeLa cells. The physiochemical characterization of phycocyanin was also conducted, revealing its structural backbone. This study introduces phycocyanin as a potentially useful anticancer and antioxidant agent and provides a new path for future researchers to combat diseases such as cancer and cardiovascular.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Green & Sustainable Science & Technology
Li Zhang, Yue You, Chundi Gao, Yongzhen Peng, Zhiguo Cao
Summary: The study found that the overlying water of the Beiyun River mainly consists of lignin and protein, with low lignin content and high protein content making the DON unstable. Upstream to downstream, lignin remains stable, while lipids and condensed aromatics are unstable.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Plant Sciences
Jing-Wei Li, Yu-Meng Zhang, Chun-Jie Zhao, Min Zhao, Yi-He Huang
Summary: This study quickly identified the blood components of danyikangtai powder and evaluated its therapeutic effect on pancreatitis. It provides a scientific reference for its pharmacological research and rational clinical application.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2023)
Article
Engineering, Environmental
Yingxin Shang, Zhidan Wen, Kaishan Song, Ge Liu, Fengfa Lai, Lili Lyu, Sijia Li, Hui Tao, Junbin Hou, Chong Fang, Chen He, Quan Shi, Ding He
Summary: This study demonstrates the diverse sources and compositions for humic-rich lakes and protein-rich lakes in large-scale regions across China, linking them to optical components and molecular high-resolution mass spectrometry properties.
Article
Engineering, Environmental
Yingxin Shang, Zhidan Wen, Kaishan Song, Ge Liu, Fengfa Lai, Lili Lyu, Sijia Li, Hui Tao, Junbin Hou, Chong Fang, Chen He, Quan Shi, Ding He
Summary: DOM is crucial for the global carbon biogeochemical cycle in aquatic ecosystems, with diverse sources and compositions across different lake regions. Humic-rich lakes exhibit more complex molecular compositions and higher aromaticity compared to protein-rich lakes.
Article
Engineering, Environmental
A. Butturini, P. Herzsprung, O. J. Lechtenfeld, P. Alcorlo, R. Benaiges-Fernandez, M. Berlanga, J. Boadella, Z. Freixinos Campillo, R. M. Gomez, M. M. Sanchez-Montoya, J. Urmeneta, A. M. Romani
Summary: This study investigates the availability and quality of dissolved organic matter (DOM) in the water column of hypersaline endorheic aquatic systems (H-SEAS). The research finds that during a hydrological wetting-drying-rewetting cycle, the DOM pool undergoes significant alterations in molecular composition, with the release of organic solutes originating from decaying microplankton under large osmotic stress at extremely high salinities.
Article
Energy & Fuels
Lidya Cardozo da Silva, Jeferson Valencia Davila, Felipe Pereira Fleming, Marianny Yajaira Combariza, Boniek Gontijo Vaz
Summary: In this study, laser desorption ionization (LDI) and collision-induced dissociation (CID) experiments were introduced as effective tools for characterizing asphaltene structures. These tools facilitated the analysis of asphaltene samples enriched in different structures and proposed corresponding structures. Additionally, correlations between asphaltene structures and their stability were attempted to be established through calculating the relative percentages of product ions.
Article
Energy & Fuels
Yi Qiu, Dian Zhong, Kuo Zeng, Jun Li, Gilles Flamant, Ange Nzihou, Haiping Yang, Hanping Chen
Summary: This study investigated the influence of cellulose-lignin interaction on the evolution of biomass pyrolysis bio-oil heavy components. The results showed that the interaction between cellulose and lignin promoted the generation of lipids and unsaturated hydrocarbons while inhibiting the generation of condensed aromatic hydrocarbons, phenolic species, and sugars.
Article
Microbiology
Christian Ayala-Ortiz, Nathalia Graf-Grachet, Viviana Freire-Zapata, Jane Fudyma, Gina Hildebrand, Roya AminiTabrizi, Cristina Howard-Varona, Yuri E. Corilo, Nancy Hess, Melissa B. Duhaime, Matthew B. Sullivan, Malak M. Tfaily
Summary: MetaboDirect is an open-source, command-line-based pipeline for the analysis, visualization, and presentation of FT-ICR MS data sets. It can generate a wide range of plots with a single line of code and automatically generate biochemical transformation networks based on mass differences.
Article
Food Science & Technology
Ane Arrizabalaga-Larranaga, Guillem Campmajo, Javier Saurina, Oscar Nunez, Francisco Javier Santos, Encarnacion Moyano
Summary: The study used the UHPLC-APCI-HRMS method for the geographical origin authentication of paprika based on capsaicinoids and carotenoids determination, achieving an authentication rate of 80.9%.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Dermatology
Simona Pisanti, Teresa Mencherini, Tiziana Esposito, Valeria D'Amato, Tania Re, Maurizio Bifulco, Rita P. Aquino
Summary: This study critically compares medieval and modern dermocosmetics through the analysis of Trotula de Ruggiero's works. The results highlight Trotula's focus on female care, beauty and well-being, as well as the extraordinary combination of tradition and modernity in her work.
JOURNAL OF COSMETIC DERMATOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Giovanni Falcone, Sonja Kuth, Aldo R. Boccaccini, Rita P. Aquino, Tiziana Esposito, Paola Russo
Summary: To fully utilize the potential of 3D printing, particularly in the case of semisolid extrusion (SSE), the technological challenges need to be addressed. This study focuses on investigating the physicochemical properties of natural polymers, specifically alginate hydrogel, for 3D printing tablets that are easy to swallow. By adding sorbitol as a plasticizer, the aim is to improve the swallowability of the tablets. Different concentrations of sorbitol solution are evaluated to determine the best composition in terms of printing and technological properties. The results highlight a predictable relationship between the amount of sorbitol loaded and the residual mass of the final platforms.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Simone Di Micco, Veronica Di Sarno, Martina Rossi, Vincenzo Vestuto, Takumi Konno, Sara Novi, Mario Felice Tecce, Valeria Napolitano, Tania Ciaglia, Andrea Vitale, Isabel Maria Gomez-Monterrey, Giuseppe Bifulco, Alessia Bertamino, Carmine Ostacolo, Paolo Blasi, Alessio Fasano, Pietro Campiglia, Simona Musella
Summary: Different mechanisms contribute to cancer multidrug resistance, including increased drug efflux, enhanced cellular repair mechanisms, and alterations of drug metabolism or targets. ABCG2, a transporter protein, promotes drug efflux and causes chemotherapeutic resistance in malignant cells. Through a multidisciplinary approach, a new selective inhibitor (8) of ABCG2 has been identified, which effectively improves the cytotoxicity of mitoxantrone in hepatocellular carcinoma and transfected breast cancer cell lines. This inhibitor also demonstrates its efficacy in reversing multidrug resistance with the overexpression of the ABCG2 pump.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Vincenzo Vestuto, Veronica Di Sarno, Simona Musella, Giorgio Di Dona, Ornella Moltedo, Isabel Maria Gomez-Monterrey, Alessia Bertamino, Carmine Ostacolo, Pietro Campiglia, Tania Ciaglia
Summary: The endoplasmic reticulum (ER) has various functions including calcium storage, protein synthesis, and lipid metabolism. ER stress, caused by disturbances in ER homeostasis, triggers unfolded protein response (UPR) to counteract the stress. Calcium levels are crucial in maintaining ER homeostasis and regulating cell fate, and dysregulation of calcium signaling is associated with diseases like neurodegeneration, cancer, and metabolic disorders. Recent research suggests that TRP channels play a role in UPR-mediated ER stress and can influence cell survival or death, making them a potential therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Carmela Tommasino, Giulia Auriemma, Carla Sardo, Carmen Alvarez-Lorenzo, Emilia Garofalo, Silvana Morello, Giovanni Falcone, Rita P. Aquino
Summary: In this study, a PCL/INU-PLA hybrid biomaterial was prepared, which showed improved surface properties, swelling ability, and in vitro biodegradation rate compared to PCL alone. The 3D printed scaffolds exhibited dimensional and structural parameters close to the digital model and showed favorable results in biocompatibility and osteogenic activity tests.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Simona Musella, Danilo D'Avino, Lukas Klaus Peltner, Veronica Di Sarno, Ida Cerqua, Fabrizio Merciai, Vincenzo Vestuto, Tania Ciaglia, Gerardina Smaldone, Francesca Di Matteo, Simone Di Micco, Valeria Napolitano, Giuseppe Bifulco, Giacomo Pepe, Eduardo Maria Sommella, Manuela Giovanna Basilicata, Giovanna Aquino, Isabel M. M. Gomez-Monterrey, Pietro Campiglia, Carmine Ostacolo, Fiorentina Roviezzo, Oliver Werz, Antonietta Rossi, Alessia Bertamino
Summary: This study describes the rational development of soluble epoxide hydrolase (sEH) inhibitors for the treatment of acute pancreatitis (AP). The most promising compound, 28, was identified through in vitro screening and molecular modeling studies. Compound 28 demonstrated remarkable efficacy in reducing inflammatory damage in a mouse model of cerulein-induced AP. Metabololipidomic analysis further confirmed the molecular mechanism of sEH inhibition in the anti-AP activity of compound 28. Overall, compound 28 shows strong effectiveness as an sEH inhibitor with potential for pharmacological treatment of AP.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Marco Persico, Paola Galatello, Maria Grazia Ferraro, Carlo Irace, Marialuisa Piccolo, Avazbek Abduvakhidov, Oleh Tkachuk, Maria Luisa d'Aulisio Garigliota, Pietro Campiglia, Patrizia Iannece, Michela Varra, Anna Ramunno, Caterina Fattorusso
Summary: A series of tetrasubstituted pyrrole derivatives (TSPs) were synthesized based on a previous hypothesis and showed significant toxicity against human epithelial melanoma A375 cells. The most active compound, 10a, resulted as the most soluble among previous and present TSPs and exhibited apoptotic cell-death pathways activation. The molecular mechanism of action based on the ability of TSPs to mimic the hydrophobic side chains of protein motifs was suggested.
Review
Biochemistry & Molecular Biology
Pasquale Marino, Giacomo Pepe, Manuela Giovanna Basilicata, Vincenzo Vestuto, Stefania Marzocco, Giuseppina Autore, Alfredo Procino, Isabel Maria Gomez-Monterrey, Michele Manfra, Pietro Campiglia
Summary: Nutrition plays a crucial role in disease prevention, especially cancer. The consumption of fruits and vegetables and a sedentary lifestyle are closely linked to cancer. Nutraceuticals, with their pleiotropic effects and non-toxic behaviors, have gained attention in cancer research. Recent clinical studies highlight the efficacy of dietary phytochemicals in cancer prevention and treatment.
Article
Biochemistry & Molecular Biology
Maria Carmina Scala, Magda Marchetti, Fabiana Superti, Mariangela Agamennone, Pietro Campiglia, Marina Sala
Summary: This study aimed to synthesize a new library of peptidomimetics active against the influenza virus and conducted a preliminary screening using biophysical assays. Through biological and computational studies on the most interesting compounds, a small peptide sequence with high activity was identified, providing a good starting point for the design of new peptidomimetics and small molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Tiziana Esposito, Simona Pisanti, Rosanna Martinelli, Rita Celano, Teresa Mencherini, Tania Re, Rita P. Aquino
Summary: This study compared the metabolite profile of Couroupita guianensis bark decoction produced by Amazonian shamans with that obtained under laboratory conditions and investigated their biological properties in wound healing and inflammation. The analysis revealed the presence of polyphenolic compounds and uncommon sulphated derivatives of ellagic acid in the decoction. A new sulphated molecule was identified as the potential active compound responsible for wound healing. The decoction also showed anti-inflammatory effects and potential therapeutic applications in skin diseases.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Patrizia Picerno, Lucia Crasci, Patrizia Iannece, Tiziana Esposito, Silvia Franceschelli, Michela Pecoraro, Virgilio Giannone, Anna Maria Panico, Rita Patrizia Aquino, Maria Rosaria Lauro, Francisca Rodrigues, Ana Margarida Silva
Summary: This study evaluated the biological and technological activity of a dry aqueous extract from Sicilian almond peels, finding significant antioxidant and metalloproteinase inhibition effects. The use of Eudraguard (R) Natural improved the solubility and release of the extract and protected it from degradation and oxidation, prolonging the shelf life of the final product.
Article
Biochemistry & Molecular Biology
Valeria Capaci, Lorenzo Monasta, Michelangelo Aloisio, Eduardo Sommella, Emanuela Salviati, Pietro Campiglia, Manuela Giovanna Basilicata, Feras Kharrat, Danilo Licastro, Giovanni Di Lorenzo, Federico Romano, Giuseppe Ricci, Blendi Ura
Summary: This study utilized a multi-omics approach to investigate common dysregulated pathways in type 1 and type 2 endometrial cancer (EC) using four EC cell lines. The analysis identified shared pathways and identified specific proteins and metabolites involved in EC tumor growth. These findings provide insights into the mechanisms underlying EC development and growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
