期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 62, 期 5, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.201700917
关键词
adipocyte; exosome; glucose transport; insulin
资金
- MINECO [SAF2015-66312]
- Fondo Europeo de Desarrollo Regional (FEDER)
- CIBER obesidad (CIBERobn), Instituto de Salud Carlos III
Scope: We investigate the effects of extracellular vesicles (EVs) obtained from in vitro adipocyte cell models and from obese subjects on glucose transport and insulin responsiveness. Methods and results: EVs are isolated from the culture supernatant of adipocytes cultured under normoxia, hypoxia (1% oxygen), or exposed to macrophage conditioned media (15% v/v). EVs are isolated from the plasma of lean individuals and subjects with obesity. Cultured adipocytes are incubated with EVs and activation of insulin signalling cascades and insulin-stimulated glucose transport are measured. EVs released from hypoxic adipocytes impair insulin-stimulated 2-deoxyglucose uptake and reduce insulin mediated phosphorylation of AKT. Insulin-mediated phosphorylation of extracellular regulated kinases (ERK1/2) is not affected. EVs from individuals with obesity decrease insulin stimulated 2-deoxyglucose uptake in adipocytes (p = 0.0159). Conclusion: EVs released by stressed adipocytes impair insulin action in neighboring adipocytes.
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