Article
Biochemistry & Molecular Biology
Lei Liu, Shuyu Guo, Xing Chen, Shuhui Yang, Xi Deng, Mingxia Tu, Yufei Tao, Wenliang Xiang, Yu Rao
Summary: This study found that biofilm probiotics have better performance in immune modulation and microbiota composition, and are more active in amino acid and carbohydrate metabolism. Through the production of various metabolites, they may impact the host's intestinal microbiome and immune activity.
Article
Microbiology
Xuejiao Zhu, Baochao Fan, Shiying Song, Jie Gao, Jinzhu Zhou, Yongxiang Zhao, Weilu Guo, Xue Zhang, Jiali Qian, Mingjun Zhu, Jizong Li, Yunchuan Li, Rongli Guo, Huiying Fan, Hailong Dong, Xuehan Zhang, Bin Li
Summary: The study investigated the role of the porcine IFN-delta (PoIFN-8) family in porcine epidemic diarrhea virus (PEDV) infection and found that PoIFN-85 showed a better antiviral effect against PEDV compared to PoIFN-81. These findings provide valuable insights into the antiviral function of porcine enteric viruses and broaden our understanding of this type of interferon.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Kiramage Chathuranga, Yeseul Shin, Md Bashir Uddin, Jayoung Paek, W. A. Gayan Chathuranga, Yebin Seong, Lu Bai, Hongik Kim, Jeong Hwan Shin, Young-Hyo Chang, Jong-Soo Lee
Summary: In this study, a strain of C. butyricum was isolated from infant feces and evaluated for its physiological characteristics and antiviral efficacy. It showed resistance to bile acid, antibacterial ability, and promoted the growth of lactic acid bacteria. The C. butyricum strain also demonstrated significant antiviral effects against influenza A, Newcastle Disease Virus, and Herpes Simplex Virus in vitro. In vivo experiments on mice showed that oral administration of C. butyricum provided prophylactic effects against highly pathogenic influenza A subtypes and modulated immune responses to reduce inflammation. These findings suggest that C. butyricum has potential as a probiotic with antiviral properties.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Blanda Di Luccia, Vittoria Acampora, Anella Saggese, Viola Calabro, Maria Vivo, Tiziana Angrisano, Loredana Baccigalupi, Ezio Ricca, Alessandra Pollice
Summary: The gut microbiota plays a positive role in maintaining intestinal homeostasis through various mechanisms. This study investigated the effects of molecules secreted by Lactobacillus gasseri SF1183 on HCT116 cells and found that these molecules can reversibly reduce cell proliferation and protect cells from apoptosis. Additionally, they enhance the barrier function between cells.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Multidisciplinary
Xingtao Zhou, Duoduo Zhang, Wucheng Qi, Tao Hong, Tao Xiong, Tingqin Wu, Fang Geng, Mingyong Xie, Shaoping Nie
Summary: EPS116 promotes intestinal epithelial regeneration by increasing the number of intestinal stem cells and modulating the gut microbiota profile, thereby maintaining intestinal barrier and homeostasis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
T. Gupta, H. Kaur, S. Kapila, R. Kapila
Summary: The study showed that Lactobacillus fermentum can alleviate Escherichia coli-induced inflammatory responses by modulating immune-related gene expression, reducing overexpression of proinflammatory cytokines, and regulating the NF-kappa B signaling pathway. This probiotic not only suppresses the excessive production of pro-inflammatory cytokines, but also modulates regulatory cytokines and NF-kappa B to alleviate inflammation.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Emanuela Casula, Maria Barbara Pisano, Gabriele Serreli, Sonia Zodio, Maria Paola Melis, Giulia Corona, Adele Costabile, Sofia Cosentino, Monica Deiana
Summary: Oxidative stress and inflammation caused by oxidised lipids in the diet, such as oxysterols, are associated with the loss of intestinal barrier integrity, which is a crucial event in intestinal disorders. Probiotic lactobacilli have shown potential in improving intestinal health due to their antioxidant and anti-inflammatory properties. This study aimed to assess the ability of two commercial probiotic strains of lactobacilli to attenuate oxysterols-induced alteration of intestinal permeability in Caco-2 cells. The results indicated that both live bacteria and intracellular components of the probiotics were able to counteract the permeability changes and loss of tight junction proteins, at least partially through modulation of the p38 pathway.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Veterinary Sciences
Shilei Huang, Qiuhan Yu, Luyi Xie, Ling Ran, Kai Wang, Yang Yang, Lu Gan, Zhenhui Song
Summary: The study showed that Lactobacillus plantarum metabolites and exopolysaccharides have inhibitory effects on Porcine Epidemic Diarrhea Virus replication, with exopolysaccharides exhibiting strong antiviral properties. Exopolysaccharides can prevent virus adsorption and induce early apoptosis of infected cells.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Andrew Foey, Neama Habil, Alex Strachan, Jane Beal
Summary: This study found that the probiotic Lacticaseibacillus casei strain Shirota (LcS) selectively modulates epithelial barrier integrity, pathogen sensing, and inflammatory cytokine profile. The specific effects depend on the subset of macrophages and their activation status.
Article
Plant Sciences
Dingfa Wang, Luli Zhou, Hanlin Zhou, Guanyu Hou
Summary: The study identified alkaloids and phenolics as the main constituents in n-butanol extract of Piper sarmentosum Roxb. This extract exerted anti-inflammatory effects by regulating the NF-kappa B signaling pathway and methionine metabolism, reducing inflammatory responses induced by lipopolysaccharide in IPEC-J2 cells.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Nutrition & Dietetics
Francesca Algieri, Jose Garrido-Mesa, Teresa Vezza, Maria Jesus Rodriguez-Sojo, Maria Elena Rodriguez-Cabezas, Monica Olivares, Federico Garcia, Julio Galvez, Rocio Moron, Alba Rodriguez-Nogales
Summary: The study found that different probiotics have intestinal anti-inflammatory effects, particularly with slight differences in miRNA expression. Additionally, probiotics improved colitis-associated dysbiosis, although the specific bacterial groups affected varied among the probiotics tested.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Article
Food Science & Technology
Shen Zhuang, Ke Ming, Ning Ma, Jinrui Sun, Dehai Wang, Mingxing Ding, Yi Ding
Summary: A specific polysaccharide fraction (POPZ) from Portulaca oleracea L. was found to inhibit LPS-induced barrier injury and inflammatory responses in porcine intestinal epithelial monolayer, possibly through inactivating TLR4/NF-κB pathway and activating EGF/EGFR pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Agriculture, Dairy & Animal Science
Rujiu Hu, Hua Lin, Mimi Wang, Yuezhen Zhao, Haojing Liu, Yuna Min, Xiaojun Yang, Yupeng Gao, Mingming Yang
Summary: L. reuteri BBC3-derived EVs (LrEVs) play a regulatory role in attenuating LPS-induced inflammation in a chicken model. They improve growth performance, reduce mortality, and decrease intestinal injury. LrEVs suppress pro-inflammatory gene expression and enhance anti-inflammatory gene expression in the jejunum, indicating their potential immunoregulatory effects.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2021)
Review
Veterinary Sciences
Dengjin Chen, Lei Zhang, Shengkui Xu
Summary: This article summarizes the research progress on the pathogenesis and immune regulation strategies of Porcine circovirus 3 (PCV3), aiming to deepen the understanding of this virus and PCV3-related diseases.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Nutrition & Dietetics
Maria do Carmo Gouveia Peluzio, Mariana de Moura e Dias, J. Alfredo Martinez, Fermin I. Milagro
Summary: Kefir, a fermented food with probiotic properties, can modulate gut microbiota composition and has preventive and management effects on diseases such as obesity, diabetes mellitus, liver disease, cardiovascular disorders, immunity, and neurological disorders. Due to its reported benefits, low cost, and ease of preparation, kefir seems to be a promising approach for preventing and managing microbiota-related diseases in Latin America and globally. Further research is needed to standardize bioactive compounds in kefir and better understand the mechanisms linking kefir and intestinal microbiota modulation.
FRONTIERS IN NUTRITION
(2021)
Article
Agriculture, Dairy & Animal Science
Md. Aminul Islam, Kenji Hashiguchi, A. K. M. Humayun Kober, Kyoko Morie, Binghui Zhou, Mikado Tomokiyo, Tomoyuki Shimazu, Hisashi Aso, Julio Villena, Yoshihito Suda, Haruki Kitazawa
Summary: The study evaluated the effects of adding Lactiplantibacillus plantarum N14 fermented rakkyo pickled juice as a feed supplement on the immune-health and production performance of pigs. Results showed that using a 5% mixture of fermented rakkyo pickled juice and residual liquid through drinking water could be a cost-effective approach to improve the immune-health and production performance of pigs. This approach may contribute to the sustainable management of food waste and provide a value-added feed supplement for animal health and production.
Review
Microbiology
A. K. M. Humayun Kober, Muhammad Shahid Riaz Rajoka, Hafiza Mahreen Mehwish, Julio Villena, Haruki Kitazawa
Summary: In the past decade, the use of probiotics as feed supplements in animal production has significantly increased due to the ban on antibiotic growth promoters. Probiotics have been suggested to improve the health, immunity, growth performance, nutritional digestibility, and intestinal microbial balance of livestock. Furthermore, they can help balance the beneficial microbial population and turnover in animals by stimulating the host immune response and excluding potentially pathogenic bacteria.
Article
Biochemistry & Molecular Biology
Leonardo Albarracin, Ramiro Ortiz Moyano, Juan Martin Vargas, Bruno G. N. Andrade, Juan Cortez Zamar, Stefania Dentice Maidana, Kohtaro Fukuyama, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: In this study, the virulence and immune response of two hypermucoviscous carbapenem-resistant Klebsiella pneumoniae strains were evaluated. The results showed that LABACER01 had higher pathogenicity and caused more severe lung tissue damage compared to LABACER27. Genomic analysis revealed the presence of virulence-related genes and adhesion genes in both strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Mikado Tomokiyo, Fernanda Raya Tonetti, Hikari Yamamuro, Ryoko Shibata, Kohtaro Fukuyama, Nadia Gobbato, Leonardo Albarracin, Muhammad Shahid Riaz Rajoka, A. K. M. Humayun Kober, Wakako Ikeda-Ohtsubo, Julio Villena, Haruki Kitazawa
Summary: The study found that postimmunobiotics derived from Lactobacillus gasseri can alter the antiviral immune response of alveolar macrophages and have a protective effect against lung inflammatory damage caused by TLR3 activation.
Article
Microbiology
Stefania Dentice Maidana, Ramiro Ortiz Moyano, Juan Martin Vargas, Kohtaro Fukuyama, Shoichiro Kurata, Vyacheslav Melnikov, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: In this study, we evaluated the immunomodulatory effect of Corynebacterium pseudodiphtheriticum 090104 strain against the respiratory infection induced by hypermucoviscous carbapenemase-producing Klebsiella pneumoniae strains. The results showed that pre-treatment with 090104 strain significantly reduced lung bacterial cell counts and tissue damage, and regulated the respiratory innate immune response. This study advances the positioning of 090104 strain as a next-generation probiotic for the respiratory tract and suggests its potential as a non-antibiotic therapeutical approach.
Article
Food Science & Technology
Masahiko Suzuki, Leonardo Albarracin, Yuji Tsujikawa, Kohtaro Fukuyama, Iwao Sakane, Julio Villena, Haruki Kitazawa
Summary: This study characterized the potential probiotic properties of Lactiplantibacillus plantarum LOC1, including its immunomodulatory effects on innate immunity. The study demonstrated that LOC1 strain can modulate inflammatory response in macrophages and enhance their protective effects mediated by the stimulation of the Th1 response. Comparative genomics analysis revealed that LOC1 possesses genes related to adhesion and immunomodulation. These findings contribute to the development of immune-related functional foods containing LOC1.
Article
Agriculture, Dairy & Animal Science
Kasumi Suzuki, Hiroki Shinkai, Gou Yoshioka, Toshimi Matsumoto, Takato Takenouchi, Junji Tanaka, Masanori Shimizu, Haruki Kitazawa, Hirohide Uenishi
Summary: This study found that DNA markers in immune-genes can be used to reduce respiratory diseases caused by bacterial pathogens in pig farming.
Article
Microbiology
Ayelen Baillo, Julio Villena, Leonardo Albarracin, Mikado Tomokiyo, Mariano Elean, Kohtaro Fukuyama, Sandra Quilodran-Vega, Silvina Fadda, Haruki Kitazawa
Summary: The immunobiotic potential of L. plantarum CRL681 and CRL1506 was studied in this research. The results showed that both strains could induce the expression of IL-6 and IL-8 in intestinal epithelial cells and enhance the levels of IFN-gamma and TNF-alpha in mice. In addition, these strains could modulate the expression of inflammatory cytokines and chemokines, reduce the pathogen counts, and prevent its spread to other organs. L. plantarum CRL1506 was found to be more efficient in modulating mucosal immunity than CRL681.
Review
Microbiology
A. K. M. Humayun Kober, Sudeb Saha, Md. Aminul Islam, Muhammad Shahid Riaz Rajoka, Kohtaro Fukuyama, Hisashi Aso, Julio Villena, Haruki Kitazawa
Summary: This review article provides an overview of recent advances in the use of probiotics for the prevention and treatment of bovine mastitis. Research has shown that probiotics can suppress mammary pathogens through competitive exclusion or the production of antimicrobial compounds. Additionally, evidence suggests that probiotics can modulate the immune response of mammary glands to inflammation. These findings indicate that probiotics can be used as a preventive strategy for managing mastitis and antimicrobial resistance, while also improving milk production and animal and human health.
Article
Biochemistry & Molecular Biology
Stefania Dentice Maidana, Mariano Elean, Kohtaro Fukuyama, Yoshiya Imamura, Leonardo Albarracin, Sudeb Saha, Yoshihito Suda, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: An increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) was observed in hospitals in Norwest Argentina. In this study, it was found that the ST25 strains of K. pneumoniae can invade human intestinal epithelial cells and induce moderate inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ramiro Ortiz Moyano, Fernanda Raya Tonetti, Kohtaro Fukuyama, Mariano Elean, Mikado Tomokiyo, Yoshihito Suda, Vyacheslav Melnikov, Haruki Kitazawa, Julio Villena
Summary: Cp and BLPs can stimulate alveolar macrophages and enhance the humoral immune response induced by the commercial pneumococcal vaccine. Mice immunized with Cp + PCV or BLPs + PCV showed higher levels of specific antibodies and lower levels of pneumococcal cell counts and lung damage compared to the control group. These findings demonstrate the potential of Cp and BLPs as adjuvants to enhance the immune response against respiratory pathogens.
Article
Microbiology
Stefania Dentice Maidana, Yoshiya Imamura, Mariano Elean, Leonardo Albarracin, Keita Nishiyama, Yoshihito Suda, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: Orally administered Lacticaseibacillus rhamnosus CRL1505 enhances respiratory immunity, providing protection against respiratory viruses and Streptococcus pneumoniae. However, its effectiveness against Gram-negative bacterial infections has not been evaluated. This study aimed to assess whether Lcb. rhamnosus CRL1505 could beneficially regulate the respiratory innate immune response and improve resistance to hypermucoviscous KPC-2-producing Klebsiella pneumoniae of the sequence type 25 (ST25).
Article
Biochemistry & Molecular Biology
Mariano Elean, Fernanda Raya Tonetti, Kohtaro Fukuyama, Luciano Arellano-Arriagada, Fu Namai, Yoshihito Suda, Nadia Gobbato, Keita Nishiyama, Julio Villena, Haruki Kitazawa
Summary: In this study, nasally administered L. salivarius FFIG58 was found to modulate the innate immune response in the respiratory tract and provide long-term protection against Streptococcus pneumoniae infection. The strain enhanced the production of various immune factors and protected mice from secondary infection for up to 30 days.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mariano Elean, Leonardo Albarracin, Julio Villena, Haruki Kitazawa, Lucila Saavedra, Elvira M. Hebert
Summary: A comprehensive genomic analysis of L. delbrueckii strains was conducted, focusing on the proteolytic system and the key enzyme, cell envelope-associated proteinase (CEP). The study compared amino acid sequences and protein structures of CEPs, as well as analyzed syntenic and genomic variations among different strains. Investigation of CEP promoter region and regulatory elements provided insights into the control of proteolytic activity in lactobacilli. This study contributes to our understanding of proteolytic activity and potential applications of L. delbrueckii in the dairy industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Sudeb Saha, Kohtaro Fukuyama, Marina Debnath, Fu Namai, Keita Nishiyama, Haruki Kitazawa
Summary: This review focuses on the effect of probiotics on improving the quality of meat from small ruminants. The findings of the reviewed articles show inconsistent results due to the use of different probiotic strains, dosage rates, experiment duration, nutrition, breed, age, and animal health status. Future research should emphasize specific strains, optimal dose and days of administration, route, and mechanisms for the specific probiotic strains to host.
