Article
Multidisciplinary Sciences
Na Sun, Yuchuan Zhang, Jian Dong, Geng Liu, Zhenbin Liu, Jiamin Wang, Zilin Qiao, Jiayou Zhang, Kai Duan, Xuanxuan Nian, Zhongren Ma, Xiaoming Yang
Summary: This study explores the metabolic differences between MDCK cells with varying proliferative capabilities using untargeted and targeted metabolomics. The findings suggest that fluctuations in metabolite levels, such as choline, tryptophan, and tyrosine, may have a significant impact on cell proliferation.
Article
Cell Biology
Magdalena Kot, Ewa Mazurkiewicz, Maciej Wiktor, Wojciech Wiertelak, Antonina Joanna Mazur, Andrei Rahalevich, Mariusz Olczak, Dorota Maszczak-Seneczko
Summary: SLC35A2 deficiency promotes epithelial-to-mesenchymal transition (EMT), leading to cell morphological changes, impaired cell-cell adhesion, altered gene expression, and Golgi reorganization.
Article
Virology
Mohamed Eisa, Samar Micky, Angela Pearson
Summary: Canid herpesvirus 1 (CHV-1) infects polarized canine epithelial cells without changing their morphological protrusions. Infection can occur from both apical and basolateral sides, but higher viral titers are produced when infecting from the basolateral side. The virus remains predominantly cell-associated throughout the experiment.
Article
Biotechnology & Applied Microbiology
Hana Malenovska
Summary: The effect of ruxolitinib medium supplement and trypsin on the adaptation of IAV in MDCK cell line was investigated. The combination of ruxolitinib and trypsin facilitated the adaptation of IAV in the cell line.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Suthat Saengchoowong, Pattaraporn Nimsamer, Kritsada Khongnomnan, Witthaya Poomipak, Kesmanee Praianantathavorn, Somruthai Rattanaburi, Yong Poovorawan, Qibo Zhang, Sunchai Payungporn
Summary: This study investigated the interaction between MDCK cells-derived microRNAs and seasonal influenza viruses. The results showed that MDCK cell-derived microRNAs target viral genes in a strain-specific manner to suppress viral replication. Moreover, the use of microRNA inhibitors may facilitate the production of influenza viruses.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jiamin Wang, Lixia Liu, Di Yang, Li Zhang, Ayimuguli Abudureyimu, Zilin Qiao, Zhongren Ma
Summary: This study revealed the role of miRNA in the tumorigenic phenotype of MDCK cell line. The miRNA expression profiles of MDCK cells with different tumorigenicity were characterized and investigated. The results showed that certain miRNAs may influence the tumorigenicity of MDCK cells by regulating target genes. These findings provide important insights into the miRNA-mediated regulatory networks that control the tumorigenicities of MDCK cells.
Article
Virology
Matthew Suderman, Mariko Moniwa, Tamiru N. Alkie, Davor Ojkic, Andre Broes, Neil Pople, Yohannes Berhane
Summary: The study found that MDCK-SIAT1 cells had the highest sensitivity for isolating IAV-S from clinical specimens, especially nasal swabs and lung tissues. Samples with lower PCR cycle threshold values were more suitable for virus isolation.
Article
Biochemistry & Molecular Biology
Minki Chang, Kevin Montagne, Katsuko S. Furukawa, Takashi Ushida
Summary: In this study, the mechanism of Ca2+ signaling induced by autocrine ATP release during cell membrane deformation by cell poking was investigated. The results revealed differences between cell poking and conventional ATP stimulation in the pathways for supplying Ca2+ into the cytoplasm. It was also found that GPCR are activated by both ATP-only stimulation and the mechanical effects of cell poking.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Nanoscience & Nanotechnology
Yun-Feng Liu, Yong-Mei Guan, Shi-Yu Huang, Lu Wu, Wei-Feng Zhu, Li-Hua Chen
Summary: The study demonstrated that paeoniflorin and albiflorin can enhance the efflux of aconitine and hypaconitine, reducing their intracellular accumulation and attenuating their cytotoxic effects.
Article
Cell Biology
Mohamed Eisa, Hamza Loucif, Julien van Grevenynghe, Angela Pearson
Summary: CHV-1 enters epithelial cells via a macropinocytosis-like pathway. It induces extensive membrane ruffling and does not depend on pH for entry into MDCK cells. The virus does not enhance fluid uptake during infection.
CELLULAR MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Sean R. Coyne, Greg M. Landry
Summary: The study aimed to evaluate the effect of tartaric acid on MDCK and HK-2 cells, and found that tartaric acid had a greater toxicity to MDCK cells. Inhibition of tartaric acid uptake through OAT-1 can effectively reduce the cytotoxicity. Additionally, transfection of hOAT-4 gene can increase the efflux of tartaric acid through OAT-4, thereby reducing cytotoxicity.
JOURNAL OF VETERINARY EMERGENCY AND CRITICAL CARE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Fabio R. M. Leite, Gustavo G. Nascimento, Holger J. Moller, Georgios N. Belibasakis, Nagihan Bostanci, Patricio C. Smith, Rodrigo Lopez
Summary: The study found that fast gingivitis development was associated with higher levels of non-organized response and lower levels of organized response, while slow responders were not associated with gingivitis. The results suggest that individual variability may play a role in susceptibility to gingivitis.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Review
Radiology, Nuclear Medicine & Medical Imaging
Pankaj Gupta, Usha Dutta, Pratyaksha Rana, Manphool Singhal, Ajay Gulati, Naveen Kalra, Raghuraman Soundararajan, Daneshwari Kalage, Manika Chhabra, Vishal Sharma, Vikas Gupta, Thakur Deen Yadav, Lileshwar Kaman, Santosh Irrinki, Harjeet Singh, Yashwant Sakaray, Chandan Krishuna Das, Uma Saikia, Ritambhara Nada, Radhika Srinivasan, Manavjit Singh Sandhu, Raju Sharma, Nitin Shetty, Anu Eapen, Harmeet Kaur, Avinash Kambadakone, Robbert de Haas, Vinay K. Kapoor, Savio George Barreto, Atul K. Sharma, Amol Patel, Pramod Garg, Sujoy K. Pal, Mahesh Goel, Shraddha Patkar, Anu Behari, Anil K. Agarwal, Bhawna Sirohi, Milind Javle, Giuseppe Garcea, Flavio Nervi, Volkan Adsay, Juan Carlos Roa, Ho-Seong Han
Summary: The GB-RADS ultrasound risk stratification system categorizes gallbladder wall thickening into six categories based on features such as symmetry, layered appearance, intramural features, and interface with the liver. This collaborative effort aims to improve the accuracy of detecting malignant lesions and enhance management strategies for gallbladder conditions.
ABDOMINAL RADIOLOGY
(2022)
Review
Oncology
Ismael Riquelme, Pablo Perez-Moreno, Pablo Letelier, Priscilla Brebi, Juan Carlos Roa
Summary: This article reviews the important aspects of piRNAs, including their molecular characteristics, biosynthesis processes, gene expression silencing mechanisms, and their study in GI cancer samples and cell lines. It also discusses the potential clinical utility of piRNAs as biomarkers and therapeutic targets in gastrointestinal cancers.
Article
Health Care Sciences & Services
Pablo Perez-Moreno, Ismael Riquelme, Patricia Garcia, Priscilla Brebi, Juan Carlos Roa
Summary: Gallbladder cancer is an aggressive neoplasm that is often asymptomatic in early stages and is typically diagnosed at an advanced stage. The presence of gallstones is the main risk factor, while other factors such as inflammation can also contribute to the development of gallbladder cancer. Understanding the early carcinogenic mechanisms is crucial for prevention.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Editorial Material
Immunology
Lesley Ann Bergmeier, Nicolas Dutzan, Patricio C. Smith, Heleen Kraan
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Gabriela Vargas, Omar Cortes, Eloisa Arias-Munoz, Sergio Hernandez, Cristobal Cerda-Troncoso, Laura Hernandez, Alexis E. Gonzalez, Michael H. Tatham, Hianara A. Bustamante, Claudio Retamal, Jorge Cancino, Manuel Varas-Godoy, Ronald T. Hay, Alejandro Rojas-Fernandez, Viviana A. Cavieres, Patricia V. Burgos
Summary: Macroautophagy and the ubiquitin proteasome system are interconnected in maintaining cellular homeostasis. HERPUD1, a protein regulated by proteasomal degradation, stabilizes ER and lysosomal network and negatively regulates autophagy, promoting cell survival under stress conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Susana Rios, Simon Alvarez, Patricio C. Smith, Claudia G. Saez, Catherine Andrade, Nelson Pinto, Constanza E. Martinez
Summary: This study analyzed the release of biomolecules from leucocyte- and platelet-rich fibrin (L-PRF) derived from smokers and nonsmokers, and investigated their effects on cell proliferation and migration in periodontal ligament cells. The results showed that L-PRF stimulated cell proliferation and migration, with no significant difference between smokers and nonsmokers. Nicotine inhibited cell proliferation and migration, but this effect was recovered in the presence of L-PRF conditioned media.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)
Article
Medicine, General & Internal
Juan C. Roa, Patricia Garcia, Vinay K. Kapoor, Shishir K. Maithel, Milind Javle, Jill Koshiol
Summary: Gallbladder cancer is the most common cancer of the biliary tract and often has a poor prognosis. Early detection is challenging and chronic inflammation plays a significant role in the development of this cancer. Surgical resection is the main curative treatment option, but it is only suitable for a small number of cases.
NATURE REVIEWS DISEASE PRIMERS
(2022)
Correction
Medicine, General & Internal
Juan C. Roa, Patricia Garcia, Vinay K. Kapoor, Shishir K. Maithel, Milind Javle, Jill Koshiol
NATURE REVIEWS DISEASE PRIMERS
(2022)
Review
Biochemistry & Molecular Biology
Ismael Riquelme, Pablo Perez-Moreno, Barbara Mora-Lagos, Carmen Ili, Priscilla Brebi, Juan Carlos Roa
Summary: Gastric cancer is a significant cause of cancer-related deaths, particularly among younger people. Late diagnosis, metastasis, and drug resistance contribute to the high mortality rate of gastric cancer. There is a need for new biomarkers and therapeutic targets. RNA-seq technology has identified long non-coding RNAs (lncRNAs) as potential regulators of cancer cells' signaling pathways, including the PI3K/AKT/mTOR pathway, and they may serve as diagnostic or prognostic biomarkers or therapeutic targets for gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Luis Vergara-Gomez, Carolina Bizama, Jun Zhong, Kurt Buchegger, Felipe Suarez, Lorena Rosa, Carmen Ili, Helga Weber, Javiera Obreque, Karena Espinoza, Gabriela Repetto, Juan C. Roa, Pamela Leal, Patricia Garcia
Summary: This study investigated the molecular mechanisms of acquired resistance in gallbladder cancer by developing and characterizing gemcitabine-resistant cell lines. Transcriptome profiling and phosphotyrosine proteomic analyses revealed dysregulated biological processes and signaling pathways, as well as potential therapeutic targets in gallbladder cancer. The findings greatly enhance our understanding of acquired drug resistance in gallbladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maria Elena Reyes, Louise Zanella, Ismael Riquelme, Kurt Buchegger, Barbara Mora-Lagos, Pablo Guzman, Patricia Garcia, Juan C. Roa, Carmen Gloria Ili, Priscilla Brebi
Summary: This study aimed to investigate the frequency and genetic diversity of EBV in gastric cancer (GC) patients in southern Chile. The study found that 22.2% of the GC samples were EBV-positive and significantly associated with diffuse-type histology. The results provide important information for epidemiological follow-up of different EBV subtypes that may cause GC in southern Chile.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Bahar Memis, Burcu Saka, Juan Carlos Roa, Sudeshna Bandyopadhyay, Michelle Reid, Pelin Bagci, Berk Kaan Aktas, Ayse Armutlu, Olca Basturk, N. Volkan Adsay
Summary: Eosinophilic cholecystitis is a rare condition characterized by thickening of the gallbladder wall and infiltration of eosinophils, which is most commonly seen in patients with subacute and acute cholecystitis.
Review
Biochemistry & Molecular Biology
Jose Ignacio Erices, Carolina Bizama, Ignacio Niechi, Daniel Uribe, Arnaldo Rosales, Karen Fabres, Giovanna Navarro-Martinez, Angelo Torres, Rody San Martin, Juan Carlos Roa, Claudia Quezada-Monras
Summary: Glioblastoma (GBM) is a common and malignant brain cancer with poor prognosis. Its low effectiveness is attributed to tumor infiltration into healthy brain tissue, which depends on the interaction between GBM cells and the tumor microenvironment (TME). This review highlights the use of 3D patient-derived glioblastoma organoid cultures as a new platform for studying TME and invasiveness. It discusses the mechanisms involved in GBM-TME interaction and proposes potential prognostic biomarkers and therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Constanza Bay, Maria Jose Rodriguez, Paulina Tejada, Teo Feuerhake, Juan Pablo Cruz, Nicole Le Corre, Guiliana Cordova
Summary: Pediatric tuberculosis meningitis is rare and poses a diagnostic challenge. This case report demonstrates the use of radiological findings and real-time PCR to diagnose Mycobacterium tuberculosis complex in cerebrospinal fluid and brain tissue. Despite complications, the patient responded well to tuberculosis treatment. In Chile, pediatric tuberculosis is rare with nonspecific symptoms. Brain MRI findings and CSF alterations provide early suspicion of MTBC infection.
REVISTA CHILENA DE INFECTOLOGIA
(2022)
