Article
Microbiology
Elnaz Abbasi, Ehsanollah Ghaznavi-Rad
Summary: This study investigated the frequency and antibiotic resistance patterns of MDR and CRKP in patients' infectious samples in central Iran. The findings highlight the urgent need for tracking and immediate intervention to control the high prevalence of MDR and CRKP in this region.
Article
Microbiology
Feilong Zhang, Ziyao Li, Xinmeng Liu, Guolan Luo, Yongli Wu, Chen Li, Jiankang Zhao, Yulin Zhang, Yanning Hu, Binghuai Lu
Summary: The carbapenem-resistant Klebsiella pneumoniae (CRKP) strain GX34, recovered from a patient with severe pneumonia, was found to be susceptible only to amikacin, tigecycline, and colistin. The strain belonged to K51-ST16 and carried plasmid-encoded NDM-4 and OXA-181, located on two different plasmids. The transferability and stability of these plasmids were confirmed, and comparative genomic analysis indicated a potential for dissemination of the bla(NDM-4) and bla(OXA-181) genes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Review
Infectious Diseases
Burcu Isler, Abdullah Tarik Aslan, Murat Akova, Patrick Harris, David L. Paterson
Summary: This article reviews the literature on the treatment of OXA-48-like and NDM-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) and provides updated information on available antibiotic treatment strategies. The best treatment option for OXA-48 producers is ceftazidime-avibactam, followed by colistin. There is an unmet need for the treatment of NDM producers, but ceftazidime-avibactam and aztreonam combination or cefiderocol can be used if available.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Microbiology
Yujiao Wang, Bo Zhu, Min Liu, Xiutao Dong, Jianping Ma, Xiaofeng Li, Fang Cheng, Jianzhuang Guo, Sumei Lu, Furong Wan, Yingying Hao, Wanshan Ma, Mingju Hao, Liang Chen
Summary: The study reported three Klebsiella species strains carrying TmexCD2-ToprJ2, indicating the spread of this novel plasmid-mediated tigecycline resistance mechanism to different Klebsiella species. The co-occurrence of bla(NDM-1) and TmexCD2-ToprJ2 in the same plasmid is of particular public health concern.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Stacey Ceron, Zackary Salem-Bango, Deisy A. Contreras, Elizabeth L. Ranson, Shangxin Yang
Summary: This study aimed to investigate the clinical and genomic characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) co-producing New Delhi metallo-β-lactamases (NDM) and oxacillinase 48 (OXA-48)-like carbapenemases isolated from patients in Southern California since 2016. The results showed genetic diversity in the CRKP isolates, with various lineages and antimicrobial resistance genes identified. Patients infected with this rare type of CRKP had limited treatment options and poor clinical outcomes, highlighting the importance of real-time genomic surveillance for infection prevention and treatment guidance.
Article
Infectious Diseases
Qiucheng Shi, Xinhong Han, Qin Huang, Yan Meng, Ping Zhang, Zhengan Wang, Huangdu Hu, Yan Jiang, Xiaoxing Du, Yunsong Yu
Summary: In this study, five ST307 CRKP isolates were collected and various methods were used to evaluate their antimicrobial susceptibility, clonality, and population structure. The genome characteristics and plasmid profiles were analyzed, and the plasmid transfer ability and carbapenem resistance mechanism were assessed. The results showed that ST307 is a high-risk clone type and its prevalence should be given additional attention.
Article
Microbiology
Yali Gong, Yifei Lu, Dongdong Xue, Yu Wei, Qimeng Li, Gang Li, Shuguang Lu, Jing Wang, Yunying Wang, Yizhi Peng, Yan Zhao
Summary: This study describes the emergence of a carbapenem-resistant Klebsiella pneumoniae (CRKP) strain, KP2e, in a Chinese hospital. The strain, recovered from a fatal septic shock case, carried the NDM-6 gene and exhibited resistance to almost all beta-lactams except for monobactam. It belonged to sequence type 4024 and had a genome composed of one chromosome and three plasmids. Coexistence of bla(NDM-6) on two self-transmissible plasmids, belonging to types IncFIB and IncN, was observed. The study highlights the importance of monitoring and detecting bla(NDM)-harbouring plasmids.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Arnon Chukamnerd, Rattanaruji Pomwised, Kongpop Jeenkeawpiam, Chanida Sakunrang, Sarunyou Chusri, Komwit Surachat
Summary: This study elucidates the genetic features of carbapenem-resistant Klebsiella pneumoniae (CRKP) clinical isolates from a university hospital in Thailand, indicating the presence of various antimicrobial resistance and virulence genes, as well as genomic diversity. The findings highlight the importance of whole-genome sequencing (WGS) in the rapid and accurate genomic analysis of clinical isolates of CRKP.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Immunology
Xi Li, Weizhong Wang, Xi Jin, Xiaofan Zhang, Xuehan Zou, Qiang Ma, Qingfeng Hu, Haijun Huang, Yuexing Tu
Summary: In this study, two NDM-1-producing CRKP ST11 strains carrying tmexCD2-toprJ2 gene clusters were identified. These gene clusters are likely to spread into clinical high-risk CRKP clones, exacerbating the antimicrobial resistance crisis. In addition, the co-occurrence of bla(NDM-1), bla(KPC-2), and tmexCD2-toprJ2 in a single strain was found, which will undoubtedly accelerate the formation of superdrug-resistant bacteria. Therefore, effective control measures should be implemented to prevent the further dissemination of such organisms in clinical settings.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sanjay Singh, Ashutosh Pathak, Nida Fatima, Chinmoy Sahu, Kashi Nath Prasad
Summary: OXA-48-like carbapenemases were detected in 40% of clinical isolates of Escherichia coli and Klebsiella pneumoniae. These isolates also carried other carbapenemases, extended spectrum beta-lactamases (ESBLs) and 16S rRNA methyltransferases. OXA-48 producers exhibited high clonal diversity and carried transferable plasmids. Strict surveillance and detection methods are crucial for preventing the dissemination of OXA-48-like carbapenemases.
Article
Infectious Diseases
Pilar Lumbreras-Iglesias, Maria Rosario Rodicio, Pablo Valledor, Tomas Suarez-Zarracina, Javier Fernandez
Summary: This study aimed to analyze the outer membrane porin-encoding genes in OXA-48 producing Klebsiella pneumoniae and assess their impact on ceftazidime/avibactam susceptibility, as well as to describe a screening methodology. Various OmpK36 alterations were detected in OXA-48-producing K. pneumoniae isolates, with high-level meropenem resistance. All isolates were susceptible to ceftazidime/avibactam, suggesting its potential as a choice therapy for these challenging isolates.
Article
Infectious Diseases
Polina Starkova, Irina Lazareva, Alisa Avdeeva, Ofeliia Sulian, Darya Likholetova, Vladimir Ageevets, Marina Lebedeva, Vladimir Gostev, Julia Sopova, Sergey Sidorenko
Summary: The study found that carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) is a new threat to healthcare. Analysis of nine CR-hvKp isolates from patients with nosocomial infections in Saint Petersburg revealed that eight of them carried large mosaic plasmids with resistance to carbapenems and hypervirulence, and identified four types of hybrid plasmids. The discovery of similar plasmids in distant regions suggests a wider distribution of hybrid plasmids carrying virulence and resistance genes.
Article
Infectious Diseases
Renjing Hu, Qin Li, Feiyang Zhang, Manlin Ding, Jun Liu, Yingshun Zhou
Summary: K. pneumoniae strain KPWX136 is resistant to most antibiotics, with a genome comprising a single chromosome and six plasmids. It harbors multiple resistance and virulence genes, as well as various mobile genetic elements.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Mirco Sandfort, Jorg B. Hans, Martin A. Fischer, Felix Reichert, Martina Cremanns, Jessica Eisfeld, Yvonne Pfeifer, Annika Heck, Tim Eckmanns, Guido Werner, Soren Gatermann, Sebastian Haller, Niels Pfennigwerth
Summary: German surveillance systems in 2022 have observed a rapid increase in the numbers of NDM-1 and NDM-1/OXA48 producing Klesbiella pneumoniae. Cases in Germany have shown a frequent presence in Ukraine before diagnosis. Whole genome sequencing of 200 isolates has revealed a high prevalence of sequence types ST147, ST307, ST395, and ST23, indicating clonal dissemination and onward transmission in Germany. Screening and isolating patients from Ukraine may help prevent further transmission.
Article
Microbiology
Afif Jati, Pedro Sola-Campoy, Thijs Bosch, Leo Schouls, Antoni P. A. Hendrickx, Veronica Bautista, Noelia W. Lara, Erwin M. Raangs, Belen E. Aracil, John W. A. Rossen, Alex Friedrich, Ana Navarro Riaza, Javier Canada-Garcia, Eva Ramirez de Arellano, Jesus Oteo-Iglesias, Maria Perez-Vazquez, Silvia Garcia-Cobos
Summary: This study investigated the presence of virulence factors in OXA-48-producing K. pneumoniae clinical isolates from The Netherlands and Spain. The majority of isolates shared a core set of chromosomally encoded virulence factors, with KL17, KL24, and O1/O2v1 being the most prevalent K-Locus and K/O loci combinations. Multidrug-resistant lineages ST11, ST101, and ST405 were associated with different yersiniabactin gene clusters. The study emphasizes the importance of surveillance for both antimicrobial resistance and virulence characteristics to prevent the spread of multidrug-resistant and highly virulent K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Susan Khanjani, Hadi Sedigh Ebrahim-Saraie, Yalda Malekzadegan, Mehrdad Halaji, Ali Mojtahedi
REVIEWS IN MEDICAL MICROBIOLOGY
(2020)
Article
Immunology
Susan Khanjani, Hadi Sedigh Ebrahim-Saraie, Mohammad Shenagari, Ali Ashraf, Ali Mojtahedi, Zahra Atrkar Roushan
ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
(2020)
Review
Critical Care Medicine
Meysam Hasannejad-Bibalan, Alireza Jafari, Hoda Sabati, Rajendra Goswami, Zakiyeh Jafaryparvar, Farzaneh Sedaghat, Hadi Sedigh Ebrahim-Saraie
Summary: This study aimed to determine the prevalence of type III secretion systems effectors in Iranian burn patients with Pseudomonas aeruginosa wound infection. The results showed a remarkable prevalence of ExoT producing isolates among ExoS, ExoU, ExoT, and ExoY.
Review
Infectious Diseases
Mehrdad Halaji, Awat Feizi, Arezoo Mirzaei, Hadi Sedigh Ebrahim-Saraie, Amirhossein Fayyazi, Ali Ashraf, Seyed Asghar Havaei
MICROBIAL DRUG RESISTANCE
(2020)
Review
Infectious Diseases
Alireza Jafari, Siavash Falahatkar, Kourosh Delpasand, Hoda Sabati, Hadi Sedigh Ebrahim-Saraie
MICROBIAL DRUG RESISTANCE
(2020)
Article
Virology
Abouzar Babaei, Somayeh Shatizadeh Malekshahi, Neda Pirbonyeh, Jamal Sarvari, Afagh Moattari
Article
Virology
Reza Shahriarirad, Amirhossein Erfani, Mohammad Rastegarian, Ali Zeighami, Nasir Arefkhah, Fariba Ghorbani, Jamal Sarvari, Bahador Sarkari
Article
Mathematical & Computational Biology
Tayebeh Hashempour, Behzad Dehghani, Zahra Musavi, Javad Moayedi, Zahra Hasanshahi, Jamal Sarvari, Seyed Younes Hosseini, Ebrahim Hosseini, Maryam Moeini, Shahin Merat
INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES
(2020)
Article
Microbiology
Alireza Shiri, Jamal Sarvari, Saeed Firoozi Ghahestani, Nasser Gholijani, Ali Mohammad Tamaddon, Mahroo Rastegari, Afagh Moattari, Seyed Younes Hosseini
Summary: The study found that coagulation factors have an impact on the expression of inflammatory and fibrotic genes in adenovirus, with the Ad36 complex loaded with FVII inducing a higher level of inflammation response.
CURRENT MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Fatemeh Lavaee, Mohammad Motamedifar, Ghazal Rafiee
Summary: The experimental study evaluated the antibacterial and anti-biofilm effects of photodynamic therapy with a photosensitizer and Gold nanoparticles against Streptococcus mutans. The combination showed similar effectiveness compared to routine photodynamic therapy in inhibiting bacterial growth and biofilm formation of S. mutans.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Microbiology
Seyed Sajjad Khoramrooz, Saba Eslami, Mohammad Motamedifar, Abdoolah Bazargani, Kamiar Zomorodian
Summary: This study found that most Acinetobacter baumannii isolates from clinical specimens in Shiraz, Iran showed resistance to multiple antibiotics and harbored integrons and associated gene cassettes. The distribution of different gene cassette arrays highlights the significant role of geographical issues in disseminating multidrug-resistant (MDR) isolates.
JUNDISHAPUR JOURNAL OF MICROBIOLOGY
(2021)
Article
Biology
Hossein Hemmati, Meysam Hasannejad-Bibalan, Sara Khoshdoz, Parisa Khoshdoz, Tofigh Yaghubi Kalurazi, Hadi Sedigh Ebrahim-Saraie, Soheila Nalban
BMC RESEARCH NOTES
(2020)
Article
Gastroenterology & Hepatology
Saeed Firoozi Ghahestani, Alireza Shiri, Afagh Moattari, Jamal Sarvari, Ali Moahammad Tamaddon, Seyed Younes Hosseini
CLINICAL AND EXPERIMENTAL HEPATOLOGY
(2020)
Review
Genetics & Heredity
Meysam Hasannejad-Bibalan, Arshid Yousefi Avarvand, Yalda Malekzadegan, Hoda Sabati, Mohammad Esmaeil Amini, Hadi Sedigh Ebrahim-Saraie
Review
Genetics & Heredity
Aref Chakari-Khiavi, Meysam Hasannejad-Bibalan, Fatemeh Shahriari, Forough Chakari-Khiavi, Ali Mojtahedi, Hadi Sedigh Ebrahim-Saraie