Article
Immunology
Alisha Chetty, Matthew G. G. Darby, Jamie Pillaye, A'ishah Taliep, Adam F. F. Cunningham, Matthew K. K. O'Shea, Gnatoulma Katawa, Laura E. E. Layland, Manuel Ritter, William G. C. Horsnell
Summary: Helminth-induced eosinophils accumulate around the parasite after it has left the infection site. These eosinophils have a complex role in parasite control and may contribute to long-term immunopathogenesis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Yi Qin, Milad Ashrafizadeh, Vera Mongiardini, Benedetto Grimaldi, Francesco Crea, Katja Rietdorf, Balazs Gyorffy, Daniel J. Klionsky, Jun Ren, Wei Zhang, Xianbin Zhang
Summary: The emergence of drug resistance is a major challenge for oncologists. Autophagy plays a complex physio-pathological role in cancer drug resistance, either promoting cell survival or contributing to cell death. Understanding the regulation and modulation of autophagy can lead to potential therapeutic strategies for cancer treatment.
Article
Biochemistry & Molecular Biology
Pei Li, Binghui Zeng, Weihong Xie, Xue Xiao, Ling Lin, Dongsheng Yu, Wei Zhao
Summary: In this study, a Kdf1 missense mutation knock-in mouse model was successfully constructed using CRISPR/Cas9 gene-editing technology. The enamel structure defects in the Kdf1 mutant mice were characterized, including chalky appearance, abnormal prism structure, decreased mineral density, altered crystal ordering degree, and chemical composition changes. These findings support the potential role of the KDF1 gene in the natural development of enamel.
Article
Multidisciplinary Sciences
Joel Crespo, Yi Ting Koh, Ningjie Hu, Paul A. Moore, Ezio Bonvini, Andrew L. Glasebrook, Andrea P. Martin, Robert J. Benschop
Summary: The study introduces a murine genetic knock-in model expressing both murine and humanized CD3 epsilon-exon, which is responsive to manipulation with anti-human CD3 therapy, showing normal development and immune functionality. Results indicate no gross abnormalities in thymocyte development or T cell peripheral homeostasis, with comparable immune responses to wild type mice. Additionally, a graft-vs-host disease model driven by effector T cell responses revealed a wasting disease upon transfer of huCD3 epsilon(HET) T cells, demonstrating the effectiveness of this humanized CD3 murine model in pre-clinical tests of anti-human CD3 antibodies.
Article
Multidisciplinary Sciences
Ernesto Rodriguez, Kelly Boelaars, Kari Brown, R. J. Eveline Li, Laura Kruijssen, Sven C. M. Bruijns, Thomas van Ee, Sjoerd T. T. Schetters, Matheus H. W. Crommentuijn, Joost C. van der Horst, Nicole C. T. van Grieken, Sandra J. van Vliet, Geert Kazemier, Elisa Giovannetti, Juan J. Garcia-Vallejo, Yvette van Kooyk
Summary: Changes in glycosylation during tumour progression, specifically the increased sialylation in PDAC tumour cells, can modulate immune cells such as monocyte-derived macrophages through Siglec receptors, leading to the generation of immune suppressive tumour associated macrophages.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yuxuan Xie, Ming Wang, Liang Gu, Yang Wang
Summary: The genetic modification of cattle has various applications in agriculture and biomedicine. However, random integration often results in unstable or differentially expressed exogenous genes, limiting the application and development of transgenic technologies. In this study, we efficiently integrated three targeted vectors into the cattle Rosa26 locus using CRISPR/Cas9 technology, and found that the CAG promoter had the highest activity in activating the expression of the exogenous gene.
Article
Pharmacology & Pharmacy
Yanchao Wang, Chen Zheng, Chao Zhuang, Qiang Fu, Baohong Zhang, Yanling Bian, Nianmin Qi, Jianwei Zhu
Summary: KA, a biosimilar mAb of Omalizumab, showed minimum differences compared to its originator in physicochemical, biological, pharmacological, and toxicological characteristics. In vitro and in vivo studies demonstrated comparable activity and efficacy to the reference product, suggesting the bio-similarity of KA. Clinical evaluation of KA is warranted based on these findings.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Review
Thermodynamics
Kristian Ronn, Andre Swarts, Vickey Kalaskar, Terry Alger, Rupali Tripathi, Juha Keskivali, Ossi Kaario, Annukka Santasalo-Aarnio, Rolf Reitz, Martti Larmi
Summary: The introduction of downsized, turbocharged Gasoline Direct Injection (GDI) engines in the automotive market has led to an increase in research on Low-speed Pre-ignition (LSPI) and super-knock within the last decade. LSPI is characterized by early ignition of fuel-air mixture, while super-knock is an occasional development from pre-ignition to high intensity knocking through detonation. Experimental research has included detailed approaches using different setups, and fuel and lubricant surrogates have allowed for modeling of various aspects of the phenomena. This paper provides a comprehensive review of LSPI and super-knock, discusses experimental methodologies, and suggests mitigating strategies based on fuel, oil, and engine parameters.
PROGRESS IN ENERGY AND COMBUSTION SCIENCE
(2023)
Article
Cell Biology
Dongjie Zhou, Ming-Hong Sun, Wen-Jie Jiang, Xiao-Han Li, Song-Hee Lee, Geun Heo, Jungseok Choi, Kwan-Suk Kim, Xiang-Shun Cui
Summary: In this study, the mRNA level of YME1L1 was knocked down in pig embryos and the expression patterns of YME1L1 and related proteins were examined. It was found that YME1L1 is localized in the punctate structures of mitochondria and highly expressed from the 4-cell stage. Knocking down YME1L1 led to decreased blastocyst rate and quality, mitochondrial fragmentation, excessive ROS production, lower mitochondrial membrane potential and ATP levels. Moreover, YME1L1 knockdown induced OPA1 cleavage and cytochrome c release. These findings demonstrate that YME1L1 is crucial for regulating mitochondrial fission, function, and apoptosis during porcine embryo preimplantation development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Immunology
Eline J. H. van Houtum, Christian Bull, Lenneke A. M. Cornelissen, Gosse J. Adema
Summary: Siglecs are a family of receptors that recognize sialic acid-containing glycans, expressed on most immune cells and have an important role in the tumor microenvironment. Their signaling pathway in the TME is enhanced through multiple mechanisms favoring tumor immune evasion.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rowan Pentz, M. Florencia Iulita, Adriana Ducatenzeiler, David A. Bennett, A. Claudio Cuello
Summary: The study revealed that in Alzheimer's disease, there are changes in the metabolism of NGF, leading to disease progression. Mature NGF degradation is enhanced while proNGF maturation is impaired, with these changes correlating with cognition, pathology, and cholinergic tone.
MOLECULAR PSYCHIATRY
(2021)
Review
Immunology
Yang Liu, Pan Zheng
Summary: CD24 is a small GPI-anchored glycoprotein with broad expression, which interacts with various receptors to mediate multiple physiological functions. Sialylated CD24 has been found to be a major ligand for CD33-family of Siglecs, playing a protective role in inflammatory and autoimmune diseases, metabolic disorders, and especially respiratory distress in COVID-19. The discoveries on CD24-Siglec interactions have driven translational research for the treatment of graft-vs-host diseases, cancer, COVID-19, and metabolic disorders. This mini-review provides a concise summary of the biological significance of the CD24-Siglec pathway and its potential for clinical translation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Liang Huang, Zhan-Qi Dong, Fei-Fang Dong, Xi-Bo Yu, Zhi-Gang Hu, Na-Chuan Liao, Peng Chen, Cheng Lu, Min-Hui Pan
Summary: In this study, a transgenic silkworm was generated using the CRISPR/Cas9 system to knockout the BmNPV inhibitor of apoptosis 2 (iap2) gene. Economic traits and growth tests showed no differences between the knockout strain and control groups, but a significant reduction in mortality rate and delay in onset time were observed after knocking out BmNPV iap2. Overall, gene editing of BmNPV iap2 was found to effectively inhibit BmNPV replication and proliferation, providing a new strategy for antiviral research.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Mahshid Deldar Abad Paskeh, Sepideh Mirzaei, Sima Orouei, Amirhossein Zabolian, Hossein Saleki, Negar Azami, Kiavash Hushmandi, Behzad Baradaran, Mehrdad Hashmi, Amir Reza Aref, Yavuz Nuri Ertas, Ali Zarrabi, Milad Ashrafizadeh, Saeed Samarghandian
Summary: miRNA-489 serves as a tumor suppressor in various cancers by sensitizing cancer cells to chemotherapy, inhibiting metastasis, and disrupting oncogenic pathways. Restoring miRNA-489 expression could enhance drug sensitivity in tumors and potentially serve as a diagnostic tool for cancer detection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Zhicong Li, Adrien Holzgreve, Lena M. Unterrainer, Viktoria C. Ruf, Stefanie Quach, Laura M. Bartos, Bogdana Suchorska, Maximilian Niyazi, Vera Wenter, Jochen Herms, Peter Bartenstein, Joerg-Christian Tonn, Marcus Unterrainer, Nathalie L. Albert, Lena Kaiser
Summary: This study built and evaluated a prediction model using clinical parameters and radiomic features extracted from [F-18]FET PET for survival stratification in patients with newly diagnosed IDH-wildtype glioblastoma. The model achieved high predictability of short-term survival and improved patient stratification beyond established prognostic markers.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Meeting Abstract
Allergy
Jennifer A. Regan, Rebecca Krier-Burris, Jeremy O'Sullivan, Paul Bryce, Bruce S. Bochner
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2016)
Article
Allergy
Jeremy A. O'Sullivan, Bruce S. Bochner
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Article
Allergy
Daniela J. Carroll, Jeremy A. O'Sullivan, David B. Nix, Yun Cao, Michael Tiemeyer, Bruce S. Bochner
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Article
Allergy
Jeremy A. O'Sullivan, Daniela J. Carroll, Yun Cao, Adriano N. Salicru, Bruce S. Bochner
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Article
Cell Biology
Jessica E. Bolden, Erin C. Lucas, Geyu Zhou, Jeremy A. O'Sullivan, Carolyn A. de Graaf, Mark D. McKenzie, Ladina Di Rago, Tracey M. Baldwin, Jake Shortt, Warren S. Alexander, Bruce S. Bochner, Matthew E. Ritchie, Douglas J. Hilton, Kirsten A. Fairfax
JOURNAL OF LEUKOCYTE BIOLOGY
(2018)
Article
Immunology
Frederick J. Kohlhapp, Andrew Zloza, Jeremy A. O'Sullivan, Tamson V. Moore, Andrew T. Lacek, Michael C. Jagoda, James McCracken, David J. Cole, Jose A. Guevara-Patino
JOURNAL OF IMMUNOLOGY
(2012)
Article
Chemistry, Multidisciplinary
Corwin M. Nycholat, Shiteng Duan, Eva Knuplez, Charli Worth, Mila Elich, Anzhi Yao, Jeremy O'Sullivan, Ryan McBride, Yadong Wei, Steve M. Fernandes, Zhou Zhu, Ronald L. Schnaar, Bruce S. Bochner, James C. Paulson
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Review
Cell Biology
Jeremy A. O'Sullivan, Alan T. Chang, Bradford A. Youngblood, Bruce S. Bochner
JOURNAL OF LEUKOCYTE BIOLOGY
(2020)
Article
Cell Biology
Eva Knuplez, Rebecca Krier-Burris, Yun Cao, Gunther Marsche, Jeremy O'Sullivan, Bruce S. Bochner
JOURNAL OF LEUKOCYTE BIOLOGY
(2020)
Review
Cell Biology
Bradford A. Youngblood, John Leung, Rustom Falahati, Jason Williams, Julia Schanin, Emily C. Brock, Bhupinder Singh, Alan T. Chang, Jeremy A. O'Sullivan, Robert P. Schleimer, Nenad Tomasevic, Christopher R. Bebbington, Bruce S. Bochner
Summary: Siglec-8 is a cell surface receptor with inhibitory activities on immune cells, being selectively expressed on human mast cells and eosinophils. AK002, an antibody targeting Siglec-8, has shown potential in clinical development for diseases involving mast cells and eosinophils.
Article
Immunology
Daniela J. Carroll, Yun Cao, Bruce S. Bochner, Jeremy A. O'Sullivan
Summary: Siglec-8 signals through an unexpected set of signaling molecules in IL-5-primed eosinophils to induce cell death, challenging the expectation that ITIM-bearing Siglecs signal through protein tyrosine phosphatases for their downstream functions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Piper A. Robida, Clayton H. Rische, Netali Ben-Baruch Morgenstern, Rethavathi Janarthanam, Yun Cao, Rebecca A. Krier-Burris, Wouter Korver, Alan Xu, Thuy Luu, Julia Schanin, John Leung, Marc E. Rothenberg, Joshua B. Wechsler, Bradford A. Youngblood, Bruce S. Bochner, Jeremy A. O'Sullivan
Summary: Mast cells are tissue-resident cells that contribute to allergic diseases through excessive or inappropriate cellular activation and degranulation. Siglec-6, a receptor selectively expressed by mast cells, could be a promising target for therapeutic intervention. This study found that Siglec-6 is highly expressed by human mast cells and has a potent inhibitory effect on mast cell activation, making it a potential therapeutic target for mast cell-driven diseases.
Review
Biochemistry & Molecular Biology
Bruce S. Bochner, Jeremy A. O'Sullivan, Alan T. Chang, Bradford A. Youngblood
Summary: Allergic diseases refer to a range of common conditions mediated by IgE, that can be from annoying to life-threatening. Current treatments include avoidance of allergens and the use of various medications. However, there are still unmet therapeutic needs. Siglecs, which are found on immune cells, play a role in regulating allergic and asthmatic responses. This review focuses on targeting specific Siglecs on cells like eosinophils and mast cells for therapeutic benefit.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Article
Biology
Cao Yun, Sooncheon Shin, Daniela J. Carroll, Jeremy A. O'Sullivan, Bruce S. Bochner
BMC RESEARCH NOTES
(2020)