Article
Biology
Di Peng, Koji Ando, Melina Hussmann, Marleen Gloger, Renae Skoczylas, Naoki Mochizuki, Christer Betsholtz, Shigetomo Fukuhara, Stefan Schulte-Merker, Nathan D. Lawson, Katarzyna Koltowska
Summary: In zebrafish, arterial mural cells have been identified as a crucial source of signals that guide the migration and survival of lymphatic endothelial cells (LECs), promoting the establishment of the lymphatic vascular network. The cooperative function of chemokine and growth factor signaling, particularly through the VEGFC-VEGFR3 pathway, plays a key role in LEC migration.
Article
Immunology
Sina Tadayon, Johannes Dunkel, Akira Takeda, Dominik Eichin, Reetta Virtakoivu, Kati Elima, Sirpa Jalkanen, Maija Hollmen
Summary: Clever-1, a molecule expressed on certain macrophages and lymphatic endothelial cells, plays a role in regulating DC trafficking and immune responses. Despite hindering DC entry into lymphatics, Clever-1 knockout mice showed increased MHC II expression on DCs and less tolerogenic LEC phenotype in lymph nodes, leading to stronger antigen-specific immune responses. This suggests that Clever-1 facilitates immunosuppressive interactions with migrating DCs, modulating immune responses in the draining lymph nodes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Hongtao He, Muliang Ding, Tao Li, Wenzhi Zhao, Lu Zhang, Peng Yin, Wei Zhang
Summary: This study discovers that NORAD, a non-coding RNA highly expressed in osteosarcoma cells, can be delivered to osteosarcoma cells through extracellular vesicles (EVs) released by bone mesenchymal stem cells (BMSCs), thereby promoting proliferation and invasion of osteosarcoma. NORAD is found to interact with miR-30c-5p and KLF10, and regulates the miR-30c-5p/KLF10 axis to influence the development and metastasis of osteosarcoma.
LABORATORY INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Mingda Li, Jie Jia, Shanshan Li, Baocheng Cui, Jiao Huang, Zhaoming Guo, Kun Ma, Li Wang, Changhao Cui
Summary: This study explored the effects of tendon stem cell-derived exosomes (TSC-exo) on tendon stem cells (TSCs), finding that TSC-exo were enriched in transforming growth factor beta (TGF beta) which accelerated the proliferation and migration of TSCs. Additionally, TGF beta carried by TSC-exo activated the TGF beta-Smad2/3 and ERK1/2 signaling pathways in TSCs, ultimately regulating the downstream molecule MMP2.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Pharmacology & Pharmacy
Shumin Luo, Jing Chen, Fang Xu, Huan Chen, Yiru Li, Weihua Li
Summary: This article summarizes a new approach in cancer immunotherapy, which is based on the exosomes derived from dendritic cells (DEXs). DEXs have the potential to improve therapeutic outcomes and overcome tumor cell-mediated immunosuppression. By capturing tumor antigens and promoting immune cell-dependent tumor rejection, DEXs play an important role in enhancing immune responses and overcoming immune escape by tumors.
Article
Cell Biology
Abby L. Bull, Leonard Campanello, Matt J. Hourwitz, Qixin Yang, Min Zhao, John T. Fourkas, Wolfgang Losert
Summary: Cells are able to prioritize among multiple guidance cues and regulate their migration direction by biasing the guidance cues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ranya Elsayed, Mahmoud Elashiry, Cathy Tran, Tigerwin Yang, Angelica Carroll, Yutao Liu, Mark Hamrick, Christopher W. Cutler
Summary: This study explores the therapeutic potential of human monocyte-derived dendritic cell (MoDC)-derived exosomes in modulating immune responses in gingival tissue to inhibit bone loss in periodontal disease. The results show that MoDC-derived exosomes can alter the function of dendritic cells and simulate immune responses in vitro, suggesting their potential as immunotherapeutic agents for controlling bone loss in periodontal disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jessica Filtjens, Anais Roger, Linda Quatrini, Elisabeth Wieduwild, Jordi Gouilly, Guillaume Hoeffel, Rafaelle Rossignol, Clara Daher, Guilhaume Debroas, Sandrine Henri, Claerwen M. Jones, Bernard Malissen, Laura K. Mackay, Aziz Moqrich, Francis R. Carbone, Sophie Ugolini
Summary: The study demonstrates that Nav(1.8)(+) sensory neurons play a crucial role in regulating CD8 T cell immune responses against HSV-1 infection, affecting skin damage severity and cytokine production. Sensory neurons are also involved in controlling neutrophil infiltration and dendritic cell responses, highlighting their potential as a therapeutic target.
NATURE COMMUNICATIONS
(2021)
Review
Materials Science, Multidisciplinary
Huinan Lai, Bo Gong, Jun Yin, Jin Qian
Summary: This review discusses the use of 3D printing technology to fabricate topographic cues for cell contact guidance. It covers typical 3D printing technologies, cellular responses on 3D printing topographic cues, and the mechanisms of contact guidance on cell outgrowth. The major challenges and future perspectives in this field are also discussed.
MATERIALS & DESIGN
(2022)
Article
Cell Biology
Furong Zeng, Zhizhao Chen, Rao Chen, William J. Shufesky, Mohna Bandyopadhyay, Geoffrey Camirand, Martin H. Oberbarnscheidt, Mara L. G. Sullivan, Catherine J. Baty, Mu-Qing Yang, Michel Calderon, Donna Beer Stolz, Geza Erdos, Roberta Pelanda, Todd Brennan, Sergio D. Catz, Simon C. Watkins, Adriana T. Larregina, Adrian E. Morelli
Summary: It has been found that donor MHC antigens are released from skin or cardiac allografts via extracellular vesicles (EVs) and captured by macrophages in lymph nodes or spleen, triggering B cell activation and DSA production. This unexpected role of graft-derived EVs opens up possibilities to interfere with EV biogenesis, trafficking, or function to restrict alloreactive B cell priming.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Chen Zong, Yan Meng, Fei Ye, Xue Yang, Rong Li, Jinghua Jiang, Qiudong Zhao, Lu Gao, Zhipeng Han, Lixin Wei
Summary: Using single-cell RNA sequencing, a distinct inflammation-associated subset of MSCs, AIF1(+)CSF1R(+) MSCs, was identified in the microenvironment before liver cancer occurrence. In a rat primary liver cancer model, MSCs with high SIRT1 expression promoted liver cancer occurrence by secreting CCL5 and recruiting macrophages. Furthermore, SIRT1 was shown to up-regulate CCL5 expression in MSCs through activation of the AKT/HIF1 alpha signaling axis.
Article
Geriatrics & Gerontology
Ranya Elsayed, Mahmoud Elashiry, Yutao Liu, Ana C. Morandini, Ahmed El-Awady, Mohamed M. Elashiry, Mark Hamrick, Christopher W. Cutler
Summary: As the aging population grows, chronic age-related bone degenerative diseases become more prevalent and severe. One such disease, periodontitis (PD), rises to 70.1% prevalence in Americans 65 years and older. Recent in vitro studies indicate that murine dendritic cells (DCs) and T cells are vulnerable to immune senescence caused by invasion of dysbiotic pathogen Porphyromonas gingivalis (Pg) and activation of the senescence associated secretory phenotype (SASP). Pg-induced DC exosomes transmit senescence to normal bystander DC and T cells, ablating antigen presentation. This study examines the biological significance of these findings in vivo, showing that immune senescence, compounded by advanced age and accelerated by oral dysbiosis and its induced SASP exosomes, play a pivotal role in the pathophysiology of experimental periodontitis.
Review
Physiology
Tomohiro Shiiya, Masanori Hirashima
Summary: During development, lymphatic endothelial cell (LEC) progenitors differentiate from venous endothelial cells only in limited regions of the body. LEC migration and subsequent tube formation are essential processes for the development of tubular lymphatic vascular network throughout the body. This review discusses the chemotactic factors, LEC-extracellular matrix interactions, and planar cell polarity regulating LEC migration and formation of tubular lymphatic vessels, providing insights into the molecular mechanisms underlying physiological lymphatic vascular development and lymphangiogenesis associated with pathological conditions.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Wenji Piao, Lushen Li, Vikas Saxena, Jegan Iyyathurai, Ram Lakhan, Yigang Zhang, Isadora Tadeval Lape, Christina Paluskievicz, Keli L. Hippen, Young Lee, Emma Silverman, Marina W. Shirkey, Leonardo Riella, Bruce R. Blazar, Jonathan S. Bromberg
Summary: This study reveals the regulatory role of PD-L1 signaling in the transendothelial migration of T cells and highlights the importance of PD-L1 in cell migration and immune regulation.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Michael Pamonag, Abigail Hinson, Elisha J. Burton, Nojan Jafari, Dominic Sales, Sarah Babcock, Rozlan Basha, Xiaofeng Hu, Kristopher E. Kubow
Summary: Directed cell migration is influenced by microenvironmental cues and cells' ability to modify their surroundings. This bi-directional relationship plays a fundamental role in cell migration in fibrillar environments.
Article
Urology & Nephrology
Christof Aigner, Martina Gaggl, Gunar Stemer, Michael Eder, Georg Bohmig, Renate Kain, Zoltan Prohaszka, Nora Garam, Dorottya Csuka, Raute Sunder-Plassmann, Leah Charlotte Piggott, Natalja Haninger-Vacariu, Alice Schmidt, Gere Sunder-Plassmann
Summary: The study examined the indications and outcomes of eculizumab therapy in a tertiary care nephrology center, showing that it is the treatment of choice for patients with complement gene-variant mediated TMA who do not respond to plasma therapy. However, response rates to therapy were lower in patients with secondary TMA and C3 glomerulopathy, highlighting the importance of carefully considering the decision to start therapy.
JOURNAL OF NEPHROLOGY
(2022)
Article
Hematology
Gabriel Gracia-Maldonado, Jason Clark, Matthew Burwinkel, Brenay Greenslade, Mark Wunderlich, Nathan Salomonis, Dario Leone, Evelina Gatti, Philippe Pierre, Ashish R. Kumar, Lynn H. Lee
Summary: By compiling a gene signature, we identified LAMP5 as a highly expressed membrane protein in MLL-r leukemia. Depletion of LAMP5 inhibited leukemia cell growth and modulated immune pathways.
Article
Oncology
Yajing Gao, Pekka Paivinen, Sushil Tripathi, Eva Domenech-Moreno, Iris P. L. Wong, Kari Vaahtomeri, Ashwini S. Nagaraj, Sarang S. Talwelkar, Marc Foretz, Emmy W. Verschuren, Benoit Viollet, Yan Yan, Tomi P. Makela
Summary: This study reveals that in LKB1-mutant LUAD, inactivation of AMPK leads to immune evasion, attenuated antigen presentation, and the formation of an immune suppressive tumor microenvironment.
CLINICAL CANCER RESEARCH
(2022)
Article
Rheumatology
Felix Kartnig, Daniel Mrak, Elisabeth Simader, Selma Tobudic, Helga Radner, Peter Mandl, Lisa Goeschl, Nikolaus Hommer, Margareta Mayer, Philipp Hofer, Thomas Hummel, Thomas Deimel, Irina Gessl, Antonia Puchner, Andreas Kerschbaumer, Renate Thalhammer, Alessandra Handisurya, Renate Kain, Stefan Winkler, Josef S. Smolen, Karin Stiasny, Thomas Perkmann, Helmuth Haslacher, Judith H. Aberle, Daniel Aletaha, Leonhard X. Heinz, Daniela Sieghart, Michael Bonelli
Summary: This clinical trial evaluated the immunogenicity and safety of a third COVID-19 vaccination in patients with immune-mediated inflammatory diseases (IMIDs). The results showed that the third vaccination had good immunogenicity and safety in these patients, but the effects of immunomodulatory therapy should be considered.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Mahzeiar Samadaei, Daniel Senfter, Sibylle Madlener, Karolina Uranowska, Christine Hafner, Michael Trauner, Nataliya Rohr-Udilova, Matthias Pinter
Summary: The combination of the RAD51 inhibitor B02 and sorafenib has a significant effect on reducing cell viability, colony formation ability, and invasion capacity of HCC cells. The in silico approach shows that higher expression of RAD51 is associated with shorter overall survival in HCC patients.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Immunology
Daniel Mrak, Felix Kartnig, Daniela Sieghart, Elisabeth Simader, Helga Radner, Peter Mandl, Lisa Goeschl, Philipp Hofer, Thomas Deimel, Irina Gessl, Renate Kain, Stefan Winkler, Josef S. Smolen, Thomas Perkmann, Helmuth Haslacher, Daniel Aletaha, Leonhard X. Heinz, Michael Bonelli
Summary: A study found that patients under immunosuppression experienced a rapid decline in antibodies after the third COVID-19 vaccine dose, and it remains unclear whether immunosuppressive therapy affects immune responses. Monitoring the immune response and adverse events in 50 immunosuppressed patients and 42 healthy controls, it was observed that immunosuppressed patients had significantly lower antibody levels, suggesting the need for an early fourth vaccination.
JOURNAL OF AUTOIMMUNITY
(2023)
Letter
Clinical Neurology
Sibylle Madlener, Julia Furtner, Natalia Stepien, Daniel Senfter, Lisa Mayr, Maximilian Zeyda, Leon Gramss, Barbara Aistleitner, Sabine Spiegl-Kreinecker, Elisa Rivelles, Christian Dorfer, Karl Roessler, Thomas Czech, Amedeo A. Azizi, Andreas Peyrl, Daniela Loetsch-Gojo, Leonhard Muellauer, Christine Haberler, Irene Slavc, Johannes Gojo
ACTA NEUROPATHOLOGICA
(2023)
Article
Hematology
Nyamdelger Sukhbaatar, Maria Schoeller, Stephanie Deborah Fritsch, Monika Linke, Stefanie Horer, Manuela Traeger, Mario Mazic, Stephan Forisch, Karine Gonzales, Jan Pascal Kahler, Carina Binder, Caroline Lassnig, Birgit Strobl, Mathias Mueller, Barbara Scheiber-Mojdehkar, Claudia Gundacker, Stefanie Dabsch, Renate Kain, Markus Hengstschlaeger, Steven H. L. Verhelst, Guenter Weiss, Igor Theurl, Thomas Weichhart
Summary: Iron is a crucial cellular metal for various physiological functions. Its absorption from the diet and transport by transferrin is important for iron metabolism. Regulation of iron absorption and transfer remains poorly understood. In this study, macrophage-specific deletion of Tsc2, a negative regulator of mTORC1, in mice led to defects in iron metabolism, including impaired erythropoiesis and reduced Tf saturation. This was due to an iron import block in the duodenal epithelial cells. Activation of mTORC1 in duodenal macrophages promoted Tf degradation, while depletion of macrophages increased Tf levels. Inhibition of mTORC1 or serine protease activity restored Tf levels in the Tsc2-deficient mice. These findings suggest that duodenal macrophages regulate iron transfer by controlling Tf availability.
Article
Virology
Ellen Gelpi, Sigrid Klotz, Miriam Beyerle, Sven Wischnewski, Verena Harter, Harald Kirschner, Katharina Stolz, Christoph Reisinger, Elisabeth Lindeck-Pozza, Alexander Zoufaly, Marlene Leoni, Gregor Gorkiewicz, Martin Zacharias, Christine Haberler, Johannes Hainfellner, Adelheid Woehrer, Simon Hametner, Thomas Roetzer, Till Voigtlaender, Gerda Ricken, Verena Endmayr, Carmen Haider, Judith Ludwig, Andrea Polt, Gloria Wilk, Susanne Schmid, Irene Erben, Anita Nguyen, Susanna Lang, Ingrid Simonitsch-Klupp, Christoph Kornauth, Maja Nackenhorst, Johannes Klaeger, Renate Kain, Andreas Chott, Richard Wasicky, Robert Krause, Guenter Weiss, Judith Loeffler-Rag, Thomas Berger, Patrizia Moser, Afshin Soleiman, Martin Asslaber, Roland Sedivy, Nikolaus Klupp, Martin Klimpfinger, Daniele Risser, Herbert Budka, Lucas Schirmer, Anne-Katrin Probstel, Romana Hoeftberger
Summary: We conducted a neuropathological analysis on 32 patients who died from COVID-19 in Austria between 2020 and 2021. The results showed diffuse white matter damage and microglial activation in all cases, indicating indirect brain damage rather than direct virus-specific damage. Inflammatory changes and vascular pathologies were also observed, along with silent neurodegenerative pathologies in elderly patients. These findings align with experimental data on SARS-CoV-2-related brain damage and provide a better understanding of the mechanisms underlying neurological symptoms after COVID-19.
Article
Genetics & Heredity
Sophie H. H. Schmidt, Ursula Barnas, Christof Aigner, Peter Wolf, Nicolas Kozakowski, Renate Kain, Thomas Scherer, Alice Schmidt, Gere Sunder-Plassmann
Summary: A 24-year-old man with diabetes mellitus and severe proteinuria was diagnosed with ABCC8-MODY12. Kidney biopsy showed nodular glomerulosclerosis. Dialysis and treatment with a sulfonylurea improved his glycemic control. This case highlights the risk of early-onset and severe diabetic kidney disease in patients with ABCC8-MODY12 and emphasizes the importance of timely genetic diagnosis for proper treatment and prevention of late sequelae of diabetes.
FRONTIERS IN GENETICS
(2023)
Article
Multidisciplinary Sciences
Mehmet Can Ucar, Edouard Hannezo, Emmi Tiilikainen, Inam Liaqat, Emma Jakobsson, Harri Nurmi, Kari Vaahtomeri
Summary: The authors reveal that developing lymphatic capillary networks utilize complementary branching strategies to optimize tissue coverage through a combination of experimental and theoretical approaches.
NATURE COMMUNICATIONS
(2023)
Article
Urology & Nephrology
Andre Oszwald, Raul A. Mejia-Pedroza, Helga Schachner, Christof Aigner, Andrew Rees, Renate Kain
Summary: The study used the DSP platform to analyze protein and mRNA expression in biopsies from patients with piFNGN, revealing gene expression profiles that are related to the morphological evolution of glomerular injury.
Article
Medicine, Research & Experimental
Emilia A. Korhonen, Aino Murtomaki, Sawan Kumar Jha, Andrey Anisimov, Anne Pink, Yan Zhang, Simon Stritt, Inam Liaqat, Lukas Stanczuk, Laura Alderfer, Zhiliang Sun, Emmi Kapiainen, Abhishek Singh, Ibrahim Sultan, Anni Lantta, Veli-Matti Leppanen, Lauri Eklund, Yulong He, Hellmut G. Augustin, Kari Vaahtomeri, Pipsa Saharinen, Taija Makinen, Kari Alitalo
Summary: This study reveals the involvement of Ang2 in VEGF-C-induced lymphangiogenesis through the PI3K/Akt signaling pathway in lymphatic endothelial cells. Blockade of Ang2 or deletion of Tie receptors in LECs inhibits lymphangiogenesis. The study highlights the important crosstalk between VEGF-C and Ang signaling pathways and suggests potential therapeutic approaches targeting Ang2/Tie/PI3K signaling for lymphangiogenesis.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Surgery
Anne-Kristin Schaefer, Attila Kiss, Andre Oszwald, Felix Nagel, Eylem Acar, Arezu Aliabadi-Zuckermann, Matthias Hackl, Andreas Zuckermann, Renate Kain, Andrzej Jakubowski, Peter Ferdinandy, Seth Hallstroem, Bruno K. Podesser
Summary: This study assessed the effects of a nitric oxide donor on graft injury after heart transplantation. The results showed that pre-treatment of the donor with S-NO-HSA reduced fibrosis and preserved levels of miR-126-3p and GATA2 in the myocardium.
TRANSPLANT INTERNATIONAL
(2022)
Article
Urology & Nephrology
Mladen Pavlovic, Andre Oszwald, Zeljko Kikic, Maja Carina Nackenhorst, Renate Kain, Nicolas Kozakowski
Summary: (English Summary:)
This study used digital image analysis (DIA) to evaluate interstitial fibrosis in pre-implantation biopsies. The results showed that frozen sections displayed a broader interstitial area compared to paraffin sections, and DIA was found to be more consistent and accurate in evaluating interstitial fibrosis. Additionally, DIA of interstitial fibrosis in paraffin sections was identified as an independent predictor for delayed graft function.
JOURNAL OF NEPHROLOGY
(2022)
