Article
Biotechnology & Applied Microbiology
Yue Liu, Jun-Jian Li, Hong-Yuan Li, Shi-Ming Deng, Ai-Qun Jia
Summary: In this study, analogs of hordenine demonstrated inhibitory activities against quorum sensing and biofilm formation in Pseudomonas aeruginosa and Serratia marcescens, with some analogs showing better activity than hordenine. Structure-activity relationship analysis indicated that the inhibitory activities of the analogs were associated with carbon chain length, presence or absence of an alpha,beta-C--C bond, amino group with/without lipophilic group, and hydroxyl group in benzene ring.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Review
Microbiology
Xuejie Li, Nixuan Gu, Teng Yi Huang, Feifeng Zhong, Gongyong Peng
Summary: Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative pathogenic microorganism known for its virulence factors, biofilm forming capability, and antimicrobial resistance. Despite being underestimated, it is a foodborne pathogen that can be found in various food groups. Chemical preservatives used to suppress microbial growth in food can pose food safety concerns. Therefore, finding effective and natural alternative antimicrobial agents for food processing is crucial. This review focuses on recent advances in food safety related to P. aeruginosa, including antimicrobial resistance, virulence factors, and prevention measures.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Wen-Ru Li, Tao-Hua Zeng, Zhi-Qing Zhang, Qing-Shan Shi, Xiao-Bao Xie
Summary: In this study, it was found that geraniol can inhibit the growth of Pseudomonas aeruginosa, prolong the lag phase and growth period. Geraniol also suppresses the expression of the three quorum sensing systems of P. aeruginosa, thereby attenuating the virulence factors regulated by these systems. This research is significant for improving the treatment of bacterial infections caused by P. aeruginosa.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shen Mao, Qiaoqiang Li, Zhikun Yang, Yasheng Li, Xinyi Ye, Hong Wang
Summary: In this paper, six series of benzoheterocyclic sulfoxide derivatives were designed and synthesised as Pseudomonas aeruginosa quorum sensing inhibitors. The experimental results showed that 6b significantly inhibited the formation of P. aeruginosa PAO1 biofilm without affecting the growth. Further mechanistic studies revealed that 6b mainly acted as a quorum sensing inhibitor through the las system. Silico molecular docking studies demonstrated the molecular binding between 6b and the P. aeruginosa quorum sensing receptor LasR via hydrogen bonding interactions. The structure-activity relationship and docking studies suggested the potential of 6b as anti-biofilm compounds for further studies on microbial resistance.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Microbiology
Alejandro Vasquez-Rifo, Jamie Cook, Deborah L. McEwan, Dania Shikara, Frederick M. Ausubel, Francesca Di Cara, Zhenyu Cheng
Summary: This study systematically compares the virulence of wild-type P. aeruginosa and QS mutants in selected hosts and conditions. It contributes to the long-term goal of understanding the intricate roles of different branches of the P. aeruginosa QS network in different hosts, and serves as a valuable resource for the field of host-pathogen interactions.
Article
Microbiology
Dan Yang, Suqi Hao, Ling Zhao, Fei Shi, Gang Ye, Yuanfeng Zou, Xu Song, Lixia Li, Zhongqiong Yin, Xiaoli He, Shiling Feng, Helin Chen, Yu Zhang, Yuanze Gao, Yinglun Li, Huaqiao Tang
Summary: The study found that paeonol has good anti-biofilm activity against Gram-negative bacteria and anti-QS activity, effectively reducing the virulence of P. aeruginosa. In vivo studies showed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Muzamil Ahmad Rather, Kuldeep Gupta, Manabendra Mandal
Summary: The study evaluated the inhibitory effect of methanol extract of Cuphea carthagenensis on biofilm and quorum sensing (QS) activity in Pseudomonas aeruginosa, demonstrating a significant suppression of biofilm formation and virulence factors.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Infectious Diseases
Nosipho Wendy S'thebe, Jamiu Olaseni Aribisala, Saheed Sabiu
Summary: Essential oils from two sunflower cultivars (AGSUN 5102 CLP and AGSUN 5106 CLP) were found to have significant antipathogenic effects against Pseudomonas aeruginosa, making them potential candidates for novel therapeutic alternatives.
Article
Microbiology
Priyanikha Jayakumar, Alexandre R. T. Figueiredo, Rolf Kummerli
Summary: Pseudomonas aeruginosa populations undergo extensive genetic adaptation and diversification in various environments. Mutations in the quorum sensing (QS) system affect the expression of virulence factors and public goods. This study found that mutations in different QS systems lead to diverging effects on the QS trait profile, potentially impacting fitness and virulence.
Article
Immunology
Giulia Giallonardi, Morgana Letizia, Marta Mellini, Emanuela Frangipani, Nigel Halliday, Stephan Heeb, Miguel Camara, Paolo Visca, Francesco Imperi, Livia Leoni, Paul Williams, Giordano Rampioni
Summary: Pseudomonas aeruginosa is a model pathogen with multiple quorum sensing circuits that control virulence factor production and biofilm formation. The pqs QS system in P. aeruginosa synthesizes AQs including HHQ and PQS, which act as signal molecules. Transcriptomic analyses show that HHQ and PQS influence gene expression through PqsR-dependent and -independent pathways. However, HQNO has no effect on P. aeruginosa transcriptome. Mutation of pqsL in P. aeruginosa leads to autolysis in colony biofilms through the accumulation of HHQ and activation of Pf4 prophage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Christian Schuetz, Duy-Khiet Ho, Mostafa Mohamed Hamed, Ahmed Saad Abdelsamie, Teresa Roehrig, Christian Herr, Andreas Martin Kany, Katharina Rox, Stefan Schmelz, Lorenz Siebenbuerger, Marius Wirth, Carsten Boerger, Samir Yahiaoui, Robert Bals, Andrea Scrima, Wulf Blankenfeldt, Justus Constantin Horstmann, Rebekka Christmann, Xabier Murgia, Marcus Koch, Aylin Berwanger, Brigitta Loretz, Anna Katharina Herta Hirsch, Rolf Wolfgang Hartmann, Claus-Michael Lehr, Martin Empting
Summary: A new generation of quorum sensing inhibitors (QSI) is reported to effectively inhibit the virulence of Pseudomonas aeruginosa and enhance antibiotic efficacy, potentially preventing the development of antimicrobial resistance. Additionally, a significant synergistic effect of QSI in combination with tobramycin against P. aeruginosa biofilms is demonstrated, showing promising implications for adjunctive pathoblocker-mediated therapy.
Article
Chemistry, Medicinal
Yinglu Wang, Qian Zheng, Li Li, Lile Pan, Hu Zhu
Summary: Quorum sensing (QS) plays an important role in regulating the pathogenicity of bacteria, and cyclo (L-Trp-L-Ser) and its derivatives have been found to exhibit anti-QS activity, making them potential candidates for developing anti-virulence agents.
Article
Biochemistry & Molecular Biology
Megha Shah, Veronique L. Taylor, Diane Bona, Yvonne Tsao, Sabrina Y. Stanley, Sheila M. Pimentel-Elardo, Matthew McCallum, Joseph Bondy-Denomy, P. Lynne Howell, Justin R. Nodwell, Alan R. Davidson, Trevor F. Moraes, Karen L. Maxwell
Summary: The study identified a new phage anti-activator protein, Aqs1, which inhibits phage infection by suppressing the quorum-sensing master regulator LasR. Aqs1 also inhibits the bacterial type IV pilus assembly ATPase protein PilB, preventing phages from superinfecting host cells. This research highlights the potential of small phage proteins to disrupt key biological pathways and offers insights into the evolutionary dynamics between phages and their hosts.
Article
Biochemistry & Molecular Biology
Zsofia Berkl, Ildiko Fekete-Kertesz, Kata Buda, Emese Vaszita, Eva Fenyvesi, Lajos Szente, Monika Molnar
Summary: Quorum sensing (QS) is a communication process in microorganisms that coordinates their activities through producing and detecting low-molecular-weight signal molecules. Cyclodextrins (CDs) can reduce signal communication and inhibit biofilm formation in pathogenic bacteria. The size, structure, and concentration of CDs are identified as key factors influencing biofilm formation. Alpha-CD and its random methylated and polymer derivatives demonstrate outstanding inhibitory efficiency.
Review
Chemistry, Medicinal
Qiaoqiang Li, Shen Mao, Hong Wang, Xinyi Ye
Summary: The survival pressure caused by antibiotics prompts bacteria to develop drug resistance. Quorum sensing is an intercellular communication system that regulates bacterial virulence and biofilm formation. In Pseudomonas aeruginosa, the formation and diffusion of biofilm is closely related to the quorum sensing system. Quorum-sensing inhibitors can reduce bacterial toxicity and enhance sensitivity to antibiotics, making them a popular topic for research and development in anti-infection. This review provides an overview of the Pseudomonas aeruginosa quorum-sensing system and quorum-sensing inhibitors research, which will aid in the future development of novel inhibitors.
Article
Biotechnology & Applied Microbiology
Jin-Wei Zhou, Ai-Qun Jia, Huan Jiang, Pei-Li Li, Hong Chen, Xiao-Juan Tan, En-Qi Liu
Summary: The endophytic fungus Phomopsis liquidambari S47 produces a quorum sensing inhibitor, 1-(4-amino-2-hydroxyphenyl)ethanone (AHE), which significantly suppresses the secretion of virulence factors in Pseudomonas aeruginosa PAO1. AHE disrupts the QS system of PAO1, leading to enhanced oxidative stress and reduced pathogenicity of the bacteria.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Lujun Yin, Xiaodong Chen, Qi Chen, Dongqing Wei, Xiang-Yang Hu, Ai-Qun Jia
Summary: This study evaluated the effects of 12 synthesized diketopiperazines (DKPs) on Arabidopsis thaliana roots and found that they promote lateral root development and root hair formation. The DKPs enhance the polar transport of auxin and interfere with auxin receptor transporter inhibitor response protein, leading to accelerated root growth and the development of lateral roots and root hair.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huai-Zhi Luo, Huan Jiang, Bing Sun, Zhen-Nan Wang, Ai-Qun Jia
Summary: Nine new compounds were isolated from an endophytic fungus of Orychophragmus violaceus, showing varying levels of inhibitory effects on NO release, with some compounds exhibiting strong effects.
Article
Food Science & Technology
Kai-Zhong Xu, Xiao-Juan Tan, Zeng-Yan Chang, Jun-Jian Li, Ai-Qun Jia
Summary: The oxidant TBQ has the potential to be a Quorum Sensing Inhibitor (QSI) and anti-biofilm agent against Chromobacterium violaceum. It can reduce the synthesis of virulence factors and inhibit biofilm formation, improving susceptibility to antibiotics in this multi-drug resistant bacteria.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhennan Wang, Pingping Zhang, Huan Jiang, Bing Sun, Huaizhi Luo, Aiqun Jia
Summary: Breast cancer is a major cause of cancer death in women worldwide, and the commonly used drug Epirubicin can lead to cardiac toxicity. Ursolic acid has been found to enhance the sensitivity of breast cancer cells to Epirubicin by modulating the autophagy pathway. This study may provide a new therapeutic strategy for combination therapy in breast cancer.
Article
Microbiology
Jin-Wei Zhou, Peng-Cheng Ji, Huan Jiang, Xiao-Juan Tan, Ai-Qun Jia
Summary: The natural product 4-hydroxycinnamic acid (HA) isolated from the endophytic fungus Phomopsis liquidambari effectively inhibits signal-based communication (QS) in Agrobacterium tumefaciens, reducing biofilm formation and pathogenicity through the induction of oxidative stress.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Huai-Zhi Luo, Huan Jiang, Xi-Shan Huang, Ai-Qun Jia
Summary: Bacteria produce virulence factors through quorum sensing mechanism, and inhibiting this mechanism can control multi-drug resistant pathogens. Compounds isolated from an endophytic fungus in Orychophragmus violaceus showed anti-quorum sensing activity, with compound 3 contributing to the potential QS inhibitory activity.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Deng Pan, Xing-Yun Wang, Jin-Wei Zhou, Liang Yang, Abbas Khan, Dong-Qing Wei, Jun-Jian Li, Ai-Qun Jia
Summary: This study investigated the anti-virulence and anti-biofilm activities of the cinnamic acid derivative, 3-methoxycinnamic acid, against Agrobacterium tumefaciens. The results showed that 3-methoxycinnamic acid interfered with the quorum sensing system of A. tumefaciens, inhibited biofilm formation, and decreased the production of 3-oxo-octanoylhomoserine lactone. It also reduced the motility and chemotaxis of A. tumefaciens, and down-regulated virulence-related genes. These findings suggest that 3-methoxycinnamic acid has the potential to be developed as a pesticide or adjuvant for the prevention and treatment of crown gall caused by A. tumefaciens.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Chemistry, Organic
Jiang Wang, Pinzhuo Ren, Gongping Gu, Zongyou Jiang, Bolin Xiang, Shi Tang, Ai-Qun Jia
Summary: An efficient and convenient method for the construction of diverse free (N-H)-benzazepinoindoles using Pd-catalyzed C(sp(2))-H imidoylative cyclization of 3-(2-isocyanobenzyl)-1H-indoles was developed. The reaction displays a broad substrate scope and can be scaled up, providing a practical route to valuable bioactive azepinoindoles.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Correction
Chemistry, Multidisciplinary
Huai-Zhi Luo, Huan Jiang, Xi-Shan Huang, Ai-Qun Jia
FRONTIERS IN CHEMISTRY
(2022)
Article
Microbiology
Lujun Yin, Ping-Ping Zhang, Wei Wang, Shi Tang, Shi-Ming Deng, Ai-Qun Jia
Summary: Serratia marcescens (S. marcescens) is a bacterium that causes infections with high morbidity and mortality rates. Infections caused by multidrug-resistant S. marcescens have become a global public health issue. In this study, a compound called 3-phenylpropan-1-amine (3-PPA) was found to inhibit biofilm formation and virulence factors of S. marcescens NJ01, and enhance its sensitivity to the antibiotic ofloxacin. 3-PPA achieved these effects by blocking quorum sensing (QS), which regulates the pathogenicity and multidrug resistance of microorganisms. These findings suggest that 3-PPA could be used as an adjuvant with ofloxacin to overcome multidrug-resistant S. marcescens and improve the treatment of intractable infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Junsheng Liu, Zengyan Chang, Xiaosa Chang, Junjian Li, Ulrich Glebe, Ai-Qun Jia
Summary: Drug-resistant bacteria pose serious social problems due to bacterial biofilms and virulence factors. Chromobacterium violaceum has developed regulatory mechanisms for biofilm development and multidrug resistance. Quorum sensing inhibitors (QSIs) offer a promising strategy to combat antibiotic resistance, and 2-tert-butyl-1,4-benzoquinone (TBQ) has been identified as a potent QSI. The combination of zinc oxide nanoparticles (ZnO-NPs) and TBQ (ZnO-TBQ) was investigated for the treatment of Chromobacterium violaceum ATCC 12472 infection. ZnO-NPs enhance the efficacy of TBQ in inhibiting virulence factors and biofilm formation, and ZnO-TBQ reduces gene expression related to QS, limiting the infectivity of C. violaceum ATCC 12472.
Article
Chemistry, Medicinal
Jia He, Luzhen Wang, DaZhao Mi, Tian Guan, Wenjing Liu, Peng He, Haijun Gu, Yuzhan Li, Yangrui Peng, Ai-Qun Jia, Ceshi Chen, Yihua Chen
Summary: JH530 is a drug that can induce cell death in triple-negative breast cancer cells and has shown significant suppression of tumor growth in vitro and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Lujun Yin, Yingjie Wang, Shiliang Xiang, Kaizhong Xu, Bo Wang, Ai-Qun Jia
Summary: In this study, it was found that tyramine could inhibit the production of virulence factors in Burkholderia cenocepacia by blocking the quorum sensing systems. It was also shown to have low toxicity and potential as a combination therapy with traditional antibiotics.
BIOCHEMICAL PHARMACOLOGY
(2023)
