Article
Chemistry, Applied
Zeliang Zhu, Yi Yuan, Jingke Guo, Xiaolin Luo, Shutao Liu, Song Miao
Summary: The study developed chitosan-coated alginate gel particles (CS/SA) and chitosan-coated alginate-shellac gel particles (CS/SA/Lac) as potential oral delivery systems for enhanced stability and activity of superoxide dismutase (SOD). The encapsulation efficiency, enzyme activity retention ratio, particle structure, SOD release behavior, and the effect of shellac were evaluated. The optimal formula for CS/SA was determined, and the addition of shellac improved SOD stability in gastric conditions. CS/SA released SOD in stimulated intestinal fluid, while CS/SA/Lac released SOD in stimulated colonic fluid. These findings suggest that CS/SA and CS/SA/Lac are excellent candidates for SOD oral administration in developing functional products.
FOOD HYDROCOLLOIDS
(2023)
Article
Nanoscience & Nanotechnology
Hae-Ji Kang, Jiaoyang Li, Md Abdur Razzak, Gi-Deok Eom, Keon-Woong Yoon, Jie Mao, Ki-Back Chu, Hui Jin, Shin Sik Choi, Fu-Shi Quan
Summary: Lessons from the recent COVID-19 pandemic highlight the importance of rapidly developing and administering an effective vaccine. In this study, a chitosan-alginate complex carrier system was designed to encapsulate an inactivated influenza virus vaccine and evaluated for oral administration in mice. The nanocomposite vaccines demonstrated successful delivery of the vaccine, induction of virus-specific antibodies, and enhanced T-cell responses. These results suggest that chitosan-alginate polymeric nanocomposites hold promise as delivery systems for oral influenza vaccines.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Fan Yang, Shang Shang, Mengfei Qi, Yajinjing Xiang, Lingmin Wang, Xinyi Wang, Tao Lin, Doudou Hao, Jiajia Chen, Jia Liu, Qing Wu
Summary: This review summarizes the recent advances in the application of yeast glucan particles (YGPs) in oral targeted delivery systems, including their structure, preparation methods, drug encapsulation, and characterization. It highlights the mechanism and validation of the target recognition interaction between YGPs and macrophages, and presents inspiring cases to demonstrate the promising applications of yeast cells. The future opportunities and challenges that YGPs may face are also emphasized in this article.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Shengnan Sun, Da Bao, Yongchun Zhou, Chen Cheng, Sen Zhang, Miao Zhao, Jing Guo
Summary: SA/CS-coated TiO2NPs hybrid fibers with photocatalytic self-cleaning properties, UV resistance, and enhanced tensile strength were prepared successfully. The core-shell particles were uniformly dispersed in the SA matrix, and their addition significantly increased the tensile strength of the fibers. The fibers exhibited excellent photocatalytic degradation performance towards various dyes and stains encountered in daily life. The prepared fibers have potential applications in textiles, automotive engineering, electronics, and medicine.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Fengmin Yang, Lu Meng, Sisi Lin, Feng Wu, Jinyao Liu
Summary: By encapsulating antigen-carrying pseudoviruses inside positively charged polyethyleneimine-modified yeast capsules, a robust oral delivery system has been developed, providing a versatile platform for the development of various oral vaccines.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Wenjuan Wei, Nouredine Behloul, Weiqi Wang, Sarra Baha, Zhenzhen Liu, Ruihua Shi, Jihong Meng
Summary: The study focused on developing oral vaccine nanoparticles using truncated capsid protein p146 for hepatitis E (HE) and utilized chitosan nanoparticles as a mucosal delivery system. The produced CS/p146 NPs exhibited stable dispersion, near-spherical shape with optimal size, high encapsulation efficiency, and low cytotoxicity with sustained-release effects. Immunogenicity experiments in mice showed that CS/p146 NPs induced higher antigen-specific systemic and mucosal immune responses compared to purified p146, highlighting their potential as HE oral vaccine candidates.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Polymer Science
Viktoria Milkova
Summary: This study investigates the influence of the physicochemical characteristics of alginate on the electrical properties and stability of polymer-coated colloidal particle dispersions. The results indicate that the experimental values of the frequency of relaxation of the electro-optical effect are higher than the theoretical ones, suggesting the polarization of condensed Na+ counterions at specific distances according to the molecular structure. Furthermore, the presence of divalent ions in the polymer layer affects the electro-optical behavior of particles with adsorbed alginate molecules.
Article
Chemistry, Applied
Mulham Alfatama, Lee Yong Lim, Tin Wui Wong
Summary: This study focused on designing chitosan-coated calcium alginate beads with a core-coat structure, utilizing vibratory nozzle extrusion-microencapsulation method. By enhancing the attraction between tripolyphosphate ions in the core and chitosan species, the beads demonstrated improved resistance against swelling and water uptake.
CARBOHYDRATE POLYMERS
(2021)
Article
Pharmacology & Pharmacy
Ernesto R. R. Soto, Charles A. A. Specht, Chrono K. K. Lee, Stuart M. M. Levitz, Gary R. R. Ostroff
Summary: This paper describes a new method of encapsulating His-tagged vaccine antigens using GP-Ni as a delivery vehicle, allowing for one-step binding and targeted delivery to antigen-presenting cells. The efficacy of this approach was demonstrated using Cda2 cryptococcal antigens in a mouse infection model, showing comparable results to a previous method utilizing MSA and yeast RNA trapping of Cda2 in GPs.
Article
Biotechnology & Applied Microbiology
Yonghyun Choi, Jaehee Jang, Hyung-Jun Koo, Masayoshi Tanaka, Keun-Heon Lee, Jonghoon Choi
Summary: Natural polymer materials, such as alginate and chitosan, are widely used in medical and support applications due to their high biocompatibility and ability to form stable structures. Beta-glucan plays a significant role in material stability and skin effects. This study evaluated antimicrobial activity by incorporating beta-glucan nanoemulsions into alginate-chitosan hydrogel patches, potentially leading to wound dressings with antibacterial properties.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2021)
Article
Food Science & Technology
Matheus A. S. Santos, Mariana T. C. Machado
Summary: External ionic gelation is a promising technique for protecting probiotics, as shown in this study where particles containingSaccharomyces boulardiiproduced using sodium alginate and chitosan coating were evaluated. The alginate-chitosan particles provided better protection to the yeast during storage and exhibited resistance to simulated gastrointestinal conditions, indicating their potential use to enhance food products.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dong Yang, Keyi Gao, Yang Bai, Lei Lei, Tongtong Jia, Kaidi Yang, Chaohua Xue
Summary: This study utilized a microfluidic approach to synthesize magnetic alginate microparticles and chitosan-coated magnetic alginate microparticles, achieving controlled drug release. By investigating the model drug amoxicillin, it was found that particle size affects drug release rate, while chitosan coating extended drug release time by two times.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Fisheries
Xingchen Huo, Lingjie Tang, Qian Liu, Wentao Zhu, Jingjing Zhang, Meidi Hu, Fengxia Zhao, Pengxu Wang, Gailing Yuan, Chunrong Yang, Jianguo Su
Summary: In this study, oral DNA nano vaccines were developed using chitosan/sodium alginate nanoparticles as carriers to effectively prevent grass carp hemorrhagic disease caused by grass carp reovirus. The results showed that the oral nano vaccines could enhance the fish's immune response, inhibit the replication of the virus, and have the potential to be used in the aquaculture industry for developing multiple DNA vaccines.
Article
Engineering, Biomedical
Nourhan H. AbdelAllah, Yasser Gaber, Sameh AbdelGhani, Mohamed E. Rashed, Ahmed F. Azmy
Summary: Chitosan and alginate salt, as natural adjuvants, significantly improved the immune response to the injectable killed cholera vaccine (KCV), demonstrating increased specific antibody levels, strong proliferation effects, and high protection rates against different cholera strains. These findings highlight the potential for further vaccine development.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Chemistry, Multidisciplinary
Zhe Jing, Shuqing Wang, Keyuan Xu, Qian Tang, Wenjing Li, Wei Zheng, Haobo Shi, Kailing Su, Yanting Liu, Zhangyong Hong
Summary: A safe and broad-spectrum neoantigen vaccine delivery system (GP-Neoantigen) based on beta-1,3-glucan particles (GPs) has been proposed in this study. The system induces strong immune responses and inhibits tumor growth, and can be further enhanced by using toll-like receptors (TLRs) agonists. These results provide possibilities for further clinical promotion and personalized vaccine treatment.
Article
Pharmacology & Pharmacy
Joao Panao Costa, Sofia Carvalho, Sandra Jesus, Edna Soares, Ana Patricia Marques, Olga Borges
Article
Biochemistry & Molecular Biology
Edna Soares, Rosemeyre Cordeiro, Henrique Faneca, Olga Borges
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Pharmacology & Pharmacy
Dulce Bento, Sandra Jesus, Filipa Lebre, Teresa Goncalves, Olga Borges
Article
Medicine, Research & Experimental
Edna Soares, Zwier M. A. Groothuismink, Andre Boonstra, Olga Borges
MOLECULAR PHARMACEUTICS
(2019)
Article
Immunology
Debora Botura Scariot, Helito Volpato, Nilma de Souza Fernandes, Edna Filipa Pais Soares, Tania Ueda-Nakamura, Benedito Prado Dias-Filho, Zia Ud Din, Edson Rodrigues-Filho, Adley Forti Rubira, Olga Borges, Maria Do Ceu Sousa, Celso Vataru Nakamura
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Jessica Da Silva, Sandra Jesus, Natalia Bernardi, Mariana Coleco, Olga Borges
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2019)
Review
Pharmacology & Pharmacy
Edna Soares, Frederico C. Pereira
EXPERT OPINION ON PHARMACOTHERAPY
(2019)
Review
Biotechnology & Applied Microbiology
Sandra Jesus, Melanie Schmutz, Claudia Som, Gerrit Borchard, Peter Wick, Olga Borges
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Sandra Jesus, Ana Patricia Marques, Alana Duarte, Edna Soares, Joao Panao Costa, Mariana Colaco, Melanie Schmutz, Claudia Som, Gerrit Borchard, Peter Wick, Olga Borges
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Edna Soares, Julie Reis, Mariana Rodrigues, Carlos Fontes Ribeiro, Frederico C. Pereira
Summary: Depression is closely associated with physical exercise and extracellular vesicles, which help reduce systemic inflammation. New discoveries in this field provide new insights into the diagnosis and treatment of depression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Mafalda Campeao, Luciana Fernandes, Ines R. Pita, Cristina Lemos, Syed F. Ali, Felix Carvalho, Paulo Rodrigues-Santos, Carlos A. Fontes-Ribeiro, Edna Soares, Sofia D. Viana, Frederico C. Pereira
Summary: This study found that binge exposure to MDPV did not result in evident changes in behavior, neurochemistry, and glial cells compared to METH. Further profiling of MDPV's neuropharmacological profile at different time-points, regimens, and brain regions is needed.
Article
Immunology
Joao Panao Costa, Sandra Jesus, Mariana Colaco, Alana Duarte, Edna Soares, Olga Borges
Summary: The increasing awareness of endotoxin contamination has raised important questions during the study of the mechanism of action of the vaccine adjuvants. This short communication discusses the suitability of the Limulus amebocyte lysate assay to quantify chitosan nanoparticle endotoxin contamination and its impact on in vitro immunotoxicology studies. The study found that chit nanoparticles had a masking effect on endotoxin levels, making it difficult to draw reliable conclusions about their contamination effects. Non-LF and LF Chit NPs did not induce the production of certain immune response markers. Overall, nanomaterials should be produced under endotoxin-free conditions to prevent questionable results.
Article
Engineering, Biomedical
Natalia Bernardi, Sandra Jesus, Olga Borges
Summary: The small size and large surface area of nano- and microparticles make them unique for drug delivery and have the potential to overcome limitations of conventional therapeutic medicine. Polycaprolactone (PCL) nanoparticles are widely studied due to their excellent biocompatibility and degradability, while glucan has emerged as a promising natural polymer for drug delivery. This comprehensive review summarizes the production methods, physicochemical characteristics, and immunotoxicity profiles of PCL and glucan particles developed for drug delivery.
JOURNAL OF BIOMIMETICS BIOMATERIALS AND BIOMEDICAL ENGINEERING
(2023)
Article
Pharmacology & Pharmacy
Aikaterini-Theodora Chatzitaki, Sandra Jesus, Christina Karavasili, Dimitrios Andreadis, Dimitrios G. Fatouros, Olga Borges
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)