Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Review
Food Science & Technology
Zhaolin Huang, Dawei Ni, Ziwei Chen, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Summary: Enzymes can produce high-quality food with low pollution, high function, high acceptability, and medical aid. However, most enzymes, in their native form, do not meet the industrial requirements. Sequence-based and structure-based methods are the two main strategies used for enzyme modification. Molecular Dynamics (MD) simulation, as a comprehensive technology, has been widely used for structure information analysis and enzyme modification. This review focuses on the progress and development of MD simulation, its application in various food enzymes for thermostability and catalytic improvement, and the limitations and prospects of MD simulation in food enzyme modification research.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Lilang Li, Yonghua Wang, Ruiguo Cui, Fanghua Wang, Dongming Lan
Summary: A more thermostable variant of Malassezia globose lipase (SMG1) was designed, which showed improved activity towards diacylglycerols (DAGs) and increased half-life. The immobilized M5D variant demonstrated higher DAG content and residual activity in esterification reactions.
Article
Food Science & Technology
Weijia Zhu, Liqin Qin, Youqiang Xu, Hongyun Lu, Qiuhua Wu, Weiwei Li, Chengnan Zhang, Xiuting Li
Summary: Three strategies were investigated to improve the thermostability of xylanase, including reducing surface entropy, constructing intramolecular disulfide bonds, and molecular cyclization. Molecular simulations showed that all mutants exhibited improved thermostability and catalytic efficiency compared to the wild-type enzyme.
Article
Biochemistry & Molecular Biology
Licheng Zhou, Qing Meng, Ran Zhang, Bo Jiang, Qun Wu, Jingjing Chen, Tao Zhang
Summary: In this study, a comprehensive strategy was proposed to improve the thermostability of alginate lyase with strict substrate specificity. Through sequence-based and structure-based analysis, as well as computer-aided Delta Delta Gfold value calculation, four point mutations were selected and combined to generate a mutant with increased thermostability and retained enzyme activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Multidisciplinary
Dawei Ni, Shuqi Zhang, Onur Kirtel, Wei Xu, Qiuming Chen, Ebru Toksoy Oner, Wanmeng Mu
Summary: Thermostability and enzymatic activity are crucial for industrial enzyme applications. Rational design based on sequence alignment and structure modeling improved the properties of a truncated inulosucrase, with variants exhibiting enhanced denaturation temperature and activity. Combined mutagenesis and truncation of N-terminal residues further enhanced the enzyme's stability and relative activity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Fangfang Fan, Chunyan Liu, Jiaren Cao, Changjiang Lyu, Shuai Qiu, Sheng Hu, Tingting Sun, Jiaqi Mei, Hongpeng Wang, Ye Li, Weirui Zhao, Lehe Mei, Jun Huang
Summary: Synthetic shuffling technology was used to enhance the thermostability of (R)-selective transaminase. The best mutant M14 showed improved catalytic efficiency and higher enzyme activity towards acetophenone and 1-acetylnaphthalene.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Yakun Fang, Fan Liu, Yi Shi, Ting Yang, Chaojuan Liang, Yu Xin, Guiyang Shi, Liang Zhang
Summary: This study investigated the thermostable skeleton and mechanism of action of the lipase TrLipB from Thermomicrobium roseum. The results showed that substitutions at specific sites affected the thermostability and catalytic efficiency of the enzyme.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Zhi-Yuan Bai, Shuai You, Fang Zhang, Zhi-Wei Dong, Yi-Fan Zhao, Hong-Jian Wen, Jun Wang
Summary: The study modified xylanase through rational design and used it with cellulase to synergistically degrade mulberry branches pretreated with seawater. The mutant S21Y/N318W showed improved thermostability and specific activity. Under the condition of seawater immersion pre-treatment, the highest yield of fermentable sugar and degree of synergy were achieved. This study provides an efficient method of biomass saccharification for biofuel production.
Article
Biotechnology & Applied Microbiology
Lijun Li, Wenjing Li, Jianye Gong, Yanyan Xu, Zheyu Wu, Zedong Jiang, Yi-Sheng Cheng, Qingbiao Li, Hui Ni
Summary: This study introduced a computational strategy to enhance both catalytic efficiency and thermostability of enzymes simultaneously. Mutants developed through this strategy showed improved enzyme activity and thermostability in experiments, indicating the effectiveness of the approach.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Energy & Fuels
Pingping Qu, Dongdong Li, Raudah Lazim, Rui Xu, Dunchi Xiao, Fei Wang, Xun Li, Yu Zhang
Summary: The thermostability of Thermomyces lanuginosus lipase was improved through molecular dynamics simulation and in silico mutation prediction. The engineered G91C mutant showed enhanced catalytic efficiency and increased biodiesel yield at high temperatures.
Article
Food Science & Technology
Mengliang Yang, Sai Yang, Zhiwei Deng, Yan Zhang, Zhenbo Yuan, Yijian Rao
Summary: In this study, a thermostable and catalytically improved variant of the enzyme for synthesizing rebaudioside M was obtained through mutagenesis. Molecular dynamics simulations and mechanism analysis revealed the improved thermostability and enhanced catalytic activity. This work not only provides a potential method for large-scale production of rebaudioside M, but also develops strategies to enhance enzyme thermostability.
Article
Biotechnology & Applied Microbiology
Fucheng Zhu, Guosi Li, Peipei Wei, Cheng Song, Qilin Xu, Menghua Ma, Jingbo Ma, Ping Song, Sen Zhang
Summary: The rational design of enzymes with enhanced thermostability is efficient. This study successfully improved the thermostability and synthetic activity of a metalloprotease through a rational strategy, resulting in mutants with increased stability and enhanced Z-aspartame synthesis activity. Molecular dynamics simulation revealed that increased hydrogen bond interactions and a regional hydration shell were key factors in enhancing thermostability. This research provides valuable insights for enzyme engineering and has significant implications in industrial applications.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yawei Wang, Jing Wang, Zhongqiang Zhang, Jiangke Yang, Ossi Turunen, Hairong Xiong
Summary: This study investigates the characteristics of a hyperthermostable xylanase from Thermotoga maritima. Site-directed mutagenesis was performed to improve enzyme half-life, and error-prone PCR generated a mutant with improved stability and lower K-m. Additional mutations decreased thermostability and temperature optimum while increasing K-m. The study reveals the importance of active site hydrophobicity for high activity at extreme temperatures.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Yuan Li, Liangqi Chen, Xiyu Tang, Wenhui Zhu, Aixia Ma, Changyu Shi, Jinyao Li
Summary: Food-derived nattokinase has strong thrombolytic activity and few side effects, with potential applications in medicine as an adjuvant drug for thrombosis treatment and in the development of nattokinase-rich beverages and health foods. In this study, by using thermostability-prediction algorithms, two variants (S33T and T174V) of nattokinase with increased thermostability and fibrinolytic activity were screened. These variants have the potential for industrial production and application.
Article
Biotechnology & Applied Microbiology
Sofia Magalhaes Moreira, Tiago Antonio de Oliveira Mendes, Hilario Cuquetto Mantovani
Summary: Through adaptive laboratory evolution (ALE), a variant of Streptococcus equinus (Streptococcus bovis) HC5 with enhanced bacteriocin production was successfully selected; the adapted variant exhibited characteristics such as changes in cell envelope, enhanced bacteriocin release, and increased expression levels of biosynthetic genes.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Zoology
Silvia Altoe Falqueto, Bruno Faria Pitaluga, Janaina Rosa de Sousa, Sabrina Ketrin Targanski, Mateus Gandra Campos, Tiago Antonio de Oliveira Mendes, Gilvan Ferreira da Silva, Dulce Helena Siqueira Silva, Marcos Antonio Soares
Summary: This study identified novel larvicidal agents from mutualistic or soil bacteria that effectively killed Ae. aegypti larvae without affecting non-target organisms. The study also found that culture supernatants or crude lipopeptide extracts from certain bacterial strains were able to effectively kill the larvae, with observed weak-to-non-toxic effects on mammalian cells and non-target organisms. Whole genome sequencing of the best performing bacteria strains revealed biosynthetic gene clusters related to secondary metabolism, which may provide insight into the mechanisms by which these extracts kill Ae. aegypti larvae.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Soil Science
Larissa Cassemiro Pacheco Monteiro, Christiano da Conceicao de Matos, Amarilson de Oliveira Candido, Tiago Antonio de Oliveira Mendes, Mateus Ferreira Santana, Mauricio Dutra Costa
Summary: Increases in salt concentration in the soil can alter the structure of rhizosphere microbial communities, affecting the outcomes of crop-weed ecological interactions. This study found that the interaction between maize and weeds may shift from competition to facilitation with NaCl addition, likely due to changes in the rhizosphere microbial community structure.
APPLIED SOIL ECOLOGY
(2021)
Article
Biochemical Research Methods
Murillo Peterlini Tavares, Tulio Morgan, Riziane Ferreira Gomes, Marina Quadrio Raposo Branco Rodrigues, William Castro-Borges, Sebastiao Tavares de Rezende, Tiago Antonio de Oliveira Mendes, Valeria Monteze Guimaraes
Summary: The phytopathogenic fungus Chrysoporthe cubensis has a high capacity to produce efficient enzymes for lignocellulosic biomass hydrolysis. The study identified a diverse set of enzymes in the fungal extract, indicating versatility in acting on complex biomasses. This research provides insights into lignocellulose degradation mechanisms and the potential industrial applications of C. cubensis enzymes.
JOURNAL OF PROTEOMICS
(2021)
Review
Microbiology
Higor Sette Pereira, Thaysa Leite Tagliaferri, Tiago Antonio de Oliveira Mendes
Summary: In the post-genomic era, molecular treatments and diagnostics, particularly aptamers and CRISPR-Cas, have shown promise in addressing antimicrobial resistance crisis by offering practicality, sensitivity, and flexibility. While these techniques have the potential to develop quick diagnostic tools and alternative treatments for pan-resistant bacterial infections, more research is needed to ensure their successful implementation in combating AMR. Collaboration with existing diagnostic and treatment tools is also emphasized to maximize the efficacy of aptamers and CRISPR-Cas.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Pamela B. Vilela, Alessandra S. Martins, Maria Clara V. M. Starling, Felipe A. R. de Souza, Giovana F. F. Pires, Ananda P. Aguilar, Maria Eduarda A. Pinto, Tiago A. O. Mendes, Camila C. de Amorim
Summary: This study evaluated the elimination and inactivation of resistance-conferring plasmids (RCPs) in secondary wastewater using solar photo-Fenton, showing enhanced performance compared to the dark Fenton process. Solar photo-Fenton completely removed RCPs and rapidly inactivated target RCPs, demonstrating the potential to improve wastewater quality and reduce the spread of antimicrobial resistance in the environment.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Biotechnology & Applied Microbiology
Mariana Dias, Sandra Aparecida dos Reis Louzano, Lisiane Lopes da Conceicao, Rayssa da Conceicao Fernandes, Tiago Antonio de Oliveira Mendes, Solange Silveira Pereira, Leandro Licursi de Oliveira, Maria do Carmo Gouveia Peluzio
Summary: This study investigated the adjuvant treatment of obesity through potential probiotics and antibiotics, along with caloric restriction. The treatment was able to reduce biometric measures, increase brown adipose tissue, and alter the composition of the intestinal microbiota, showing promise as a treatment for obesity.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Biochemistry & Molecular Biology
Cinthia das Dores Aguiar, Yara Luiza Coelho, Hauster Maximiler Campos de Paula, Livia Neves Santa Rosa, Luciano Sindra Virtuoso, Tiago Antonio de Oliveira Mendes, Ana Clarissa dos Santos Pires, Luis Henrique Mendes da Silva
Summary: The study investigated the dynamics and energetics of interactions between human serum albumin (HSA) and CdTe quantum dots (QDs) functionalized with different ligands, showing that the functionalization of QDs can significantly impact the kinetics and thermodynamics of interactions with HSA proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Physical
Otavio Fernandes Magalhaes, Hauster Maximiler Campos De Paula, Jaqueline de Paula Rezende, Yara Luiza Coelho, Tiago Antonio de Oliveira Mendes, Luis Henrique Mendes Da Silva, Ana Clarissa dos Santos Pires
Summary: This study investigated the dynamics and thermodynamics of lysozyme (LYS) and beta-carotene (bCAR) complex formation at physiological pH using various techniques. The results showed that the interaction between LYS and bCAR is entropically driven, with the rate of intermediate complex formation depending on the association of free molecules. Additionally, temperature-dependent changes in LYS binding sites were observed during intermediate complex formation.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Agriculture, Dairy & Animal Science
Mariana Mescouto Lopes, Thais Ribeiro Brito, Josiane Fonseca Lage, Thais Correia Costa, Marta Maria dos Santos Fontes, Nick Vergara Lopes Serao, Tiago Antonio de Oliveira Mendes, Ricardo Andrade Reis, Renata Veroneze, Fabyano Fonseca e Silva, Marcio de Souza Duarte
Summary: This study investigated the effects of supplementing rumen-protected B-vitamin blend and hydroxy trace minerals on the hepatic proteome of finishing steers. The results indicated that the supplementation altered the abundance of proteins associated with the electron transport chain and other oxidation-reduction pathways, leading to the production of reactive oxygen species and modulation of proteins linked to oxidative-damage responses for maintaining cellular homeostasis.
Article
Biochemistry & Molecular Biology
Fernanda Rodrigues Nascimento, Jefferson Viktor de Paula Barros Baeta, Andressa Antunes Prado de Franca, Maria Aparecida Braga Rocha E Oliveira, Virginia Ramos Pizziolo, Anesia Aparecida dos Santos, Tiago Antonio de Oliveira Mendes, Gaspar Diaz-Munoz, Marisa Alves Nogueira Diaz
Summary: DPBP has been found to have high in vitro antineoplastic activity against melanoma cells, inducing tumor cell death through apoptosis pathways with high selectivity. Treatment with DPBP reduced tumor volume and did not cause hepatomegaly or nephrotoxicity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Pharmacology & Pharmacy
Sandra Aparecida dos Reis Louzano, Mariana de Moura e Dias, Lisiane Lopes da Conceicao, Tiago Antonio de Oliveira Mendes, Maria do Carmo Gouveia Peluzio
Summary: The study aimed to investigate the treatment of obesity with antimicrobial and potential probiotics. Results indicated that using ceftriaxone alone or in combination with the potential probiotic reduced body fat, but the negative impact of ceftriaxone on the intestinal microbiota composition may have led to intestinal dysbiosis, destroying villi structure and reducing absorptive surface.
PHARMACOLOGICAL REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Mariana de Fatima Albuquerque Pereira, Larissa Gabriela Morais de Avila, Gabriela de Cassia Avila Alpino, Bruna Cristina dos Santos Cruz, Lucas Filipe Almeida, Jordana Macedo Simoes, Andressa Ladeira Bernardes, Iasmim Xisto Campos, Andrea de Oliveira Barros Ribon, Tiago Antonio de Oliveira Mendes, Maria do Carmo Gouveia Peluzio
Summary: This study analyzed the microbial community profile of milk kefir and its effects on metabolism, oxidative stress, and the microbiota-gut-brain axis in a mouse model. The results showed that kefir improved gut microbiota, increased production of short-chain fatty acids, and positively influenced brain health. Therefore, kefir has potential benefits for maintaining gut and brain health.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Bianca L. de Sousa, Joao P. V. Leite, Tiago A. O. Mendes, Eduardo V. V. Varejao, Anna C. S. Chaves, Junio G. da Silva, Ana P. Agrizzi, Priscila G. Ferreira, Eduardo J. Pilau, Evandro Silva, Marcelo H. dos Santos
Summary: The study synthesized analogues of compound 10 and evaluated the impact of various pharmacophoric groups on AChE inhibition. It was found that aromatic amino acids were more effective for enzymatic inhibition compared to glycine. Additionally, the increase in inhibitory activity was associated with a decrease in hydrophobicity of the molecules.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2021)
Article
Agriculture, Multidisciplinary
Denia Pires de Almeida, Isabel Samila Lima Castro, Tiago Antonio de Oliveira Mendes, Danubia Rodrigues Alves, Geleta Dugassa Barka, Pedro Ricardo Rossi Marques Barreiros, Laercio Zambolim, Ney Sussumu Sakiyama, Eveline Teixeira Caixeta
Summary: This study sequenced and characterized candidate genes from coffee plants potentially involved in resistance to coffee leaf rust, identifying two RGAs with leucine-rich repeat-like kinase (LRR-RLK) motifs. These RGAs did not correspond to any resistance gene already characterized, and showed specific expression patterns in compatible and incompatible interactions with the pathogen, providing valuable information for breeding programs and understanding disease resistance mechanisms.
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)