An effective computational‐screening strategy for simultaneously improving both catalytic activity and thermostability of α‐ l ‐rhamnosidase

Enzyme engineering: Reshaping the biocatalytic functions

(2020) Misha Ali et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enhancement in affinity of Aspergillus niger JMU-TS528 α-L-rhamnosidase (r-Rha1) by semiconservative site-directed mutagenesis of (α/α)6 catalytic domain

(2020) Lijun Li et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Improved Activity of Sulfolobus acidocaldarius Maltooligosyltrehalose Synthase through Directed Evolution

(2020) Lingqia Su et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Improving the Performance of Horseradish Peroxidase by Site-Directed Mutagenesis

(2019) Diana Humer et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Rational design to improve activity of the Est3563 esterase from Acinetobacter sp. LMB-5

(2019) Li-Quan Ren et al.   ENZYME AND MICROBIAL TECHNOLOGY

Improving the catalytic efficiency of thermostable Geobacillus stearothermophilus xylanase XT6 by single amino acid substitution

(2019) Rasha A M Azouz et al.   JOURNAL OF BIOCHEMISTRY

Protein engineering of GH11 xylanase from Aspergillus fumigatus RT-1 for catalytic efficiency improvement on kenaf biomass hydrolysis

(2019) Siti Intan Rosdianah Damis et al.   ENZYME AND MICROBIAL TECHNOLOGY

Simultaneous Enhancement of Thermostability and Catalytic Activity of a Metagenome-Derived β-Glucosidase Using Directed Evolution for the Biosynthesis of Butyl Glucoside

(2019) Bangqiao Yin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Thermal Inactivation of a Cold-Active Esterase PMGL3 Isolated from the Permafrost Metagenomic Library

(2019) M.V. Kryukova et al.   Biomolecules

Development and characterization of an α- l -rhamnosidase mutant with improved thermostability and a higher efficiency for debittering orange juice

(2018) Li Jun Li et al.   FOOD CHEMISTRY

Improving the thermostability by introduction of arginines on the surface of α-L-rhamnosidase (r-Rha1) from Aspergillus niger

(2018) Lijun Li et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Crystal structure of native α-L-rhamnosidase from Aspergillus terreus

(2018) Petr Pachl et al.   Acta Crystallographica Section D-Structural Biology

Engineering of serine protease for improved thermostability and catalytic activity using rational design

(2018) Naeem Mahmood Ashraf et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Site-directed mutagenesis of α-l-rhamnosidase from Alternaria sp. L1 to enhance synthesis yield of reverse hydrolysis based on rational design

(2016) Li Xu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Exploiting non-conserved residues to improve activity and stability of Halothermothrix orenii β-glucosidase

(2016) Sushant K. Sinha et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expression and biochemical characterization of recombinant α-l-rhamnosidase r-Rha1 from Aspergillus niger JMU-TS528

(2016) Lijun Li et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Thermostability of Enzymes from Molecular Dynamics Simulations

(2016) Tim Zeiske et al.   Journal of Chemical Theory and Computation

Thermostability enhancement of xylanase Aspergillus fumigatus RT-1

(2016) Mohd Khairul Hakimi bin Abdul Wahab et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

The coming of age of de novo protein design

(2016) Po-Ssu Huang et al.   NATURE

Truncation of the unique N-terminal domain improved the thermos-stability and specific activity of alkaline α-amylase Amy703

(2016) Zhenghui Lu et al.   Scientific Reports

Crystal structure of a novel two domain GH78 family α-rhamnosidase fromKlebsiella oxytocawith rhamnose bound

(2015) Ellis C. O'Neill et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Molecular dynamics study of enhanced Man5B enzymatic activity

(2014) Rafael C Bernardi et al.   Biotechnology for Biofuels

Molecular docking and dynamics simulation analyses unraveling the differential enzymatic catalysis by plant and fungal laccases with respect to lignin biosynthesis and degradation

(2014) Manika Awasthi et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

The Structure of aStreptomyces avermitilisα-l-Rhamnosidase Reveals a Novel Carbohydrate-binding Module CBM67 within the Six-domain Arrangement

(2013) Zui Fujimoto et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Stability Mechanisms of a Thermophilic Laccase Probed by Molecular Dynamics

(2013) Niels J. Christensen et al.   PLoS One

Industrial biotechnology – past, present and future

(2011) Roland Wohlgemuth   New Biotechnology

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Assessing the Performance of the MM/PBSA and MM/GBSA Methods. 1. The Accuracy of Binding Free Energy Calculations Based on Molecular Dynamics Simulations

(2010) Tingjun Hou et al.   Journal of Chemical Information and Modeling

Role of conformational sampling in computing mutation-induced changes in protein structure and stability

(2010) Elizabeth H. Kellogg et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Model and Molecular Dynamic Simulations of Active and Inactive Endo-β-1,4-Mannanase in Tomato Fruit

(2008) Jiahuang Li et al.   PROTEIN JOURNAL