Article
Chemistry, Applied
Alejandra Aragon-Leon, Lorena Moreno-Vilet, Marisela Gonzalez-Avila, Pedro Martin Mondragon-Cortez, Guilherme Lanzi Sassaki, Raul Balam Martinez-Perez, Rosa Maria Camacho-Ruiz
Summary: This study evaluated the production, chemical characterization, biological and technological properties of inulin biosynthesized by a halophilic archaeon. The inulin showed high production, emulsifying index, and prebiotic capacity.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Multidisciplinary
Yueqiang Xu, Yan Li, Xin You, Caixia Pei, Zhuo Wang, Siming Jiao, Xin Zhao, Xuan Lin, Yang Lu, Cheng Jin, George Fu Gao, Jianjun Li, Qi Wang, Yuguang Du
Summary: This study found that exopolysaccharides produced by the halophilic archaeon Haloarcula hispanica ATCC33960 can inhibit the binding of SARS-CoV-2 to host cells, providing a novel antiviral mechanism and a guide for designing new drugs.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Saghar Shahbazi, Mohsen Zargar, Mohammad Reza Zolfaghari, Mohammad Ali Amoozegar
Summary: Carotenoids extracted from native Iranian halophilic microorganisms have been found to possess anticancer properties, particularly bacterioruberin from strain A15, which demonstrated significant cytotoxicity against breast cancer cells.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Microbiology
Ana Sofia Vazquez-Madrigal, Alejandra Barbachano-Torres, Melchor Arellano-Plaza, Manuel Reinhart Kirchmayr, Ilaria Finore, Annarita Poli, Barbara Nicolaus, Susana De la Torre Zavala, Rosa Maria Camacho-Ruiz
Summary: This study identified seventeen halophilic archaea from the Santa Barbara saltern in Sonora, Mexico, belonging to Haloarcula, Halolamina, and Halorubrum genera. The presence of neutral lipids and different phospholipids in these strains was confirmed. Carotenoid production in response to glucose, succinic acid, and glutamic acid varied among the strains, with Halorubrum sp. M5 showing negative effects from certain carbon sources. This work highlights the diversity in growth and carotenoid production of haloarchaea under different carbon sources.
Article
Biotechnology & Applied Microbiology
Magdalena Kowalewicz-Kulbat, Krzysztof T. Krawczyk, Izabela Szulc-Kielbik, Sebastian Rykowski, Marta Denel-Bobrowska, Agnieszka B. Olejniczak, Camille Locht, Magdalena Klink
Summary: Halorhabdus rudnickae and Natrinema salaciae, isolated from a Polish salt mine and Lake Medee in the Mediterranean Sea, secrete metabolites that are active against ovarian cancer cells, including those that are resistant to cisplatin, suggesting potential new possibilities for the treatment of these gynaecological cancers.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Rin Sueda, Kento Yoshida, Masahiko Onodera, Toshiaki Fukui, Rie Yatsunami, Satoshi Nakamura
Summary: The study revealed the presence of a gene cluster analogous to the bacterial glycogen synthesis operon in the genome of the extremely halophilic archaeon Haloarcula japonica. By focusing on a GlgC homolog of Ha. japonica, the recombinant enzyme showed high activity towards specific substrates under certain conditions, indicating its potential role as a GDP-glucose pyrophosphorylase in Ha. japonica.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mar Martinez Pastor, Saaz Sakrikar, Deyra N. Rodriguez, Amy K. Schmid
Summary: Due to the lack of commercial and specifically designed rRNA depletion kits, the study of genome-scale gene expression in archaea has faced challenges. Different hybridization-based kits from different suppliers have varying efficiency in removing rRNA from archaea. The selection of appropriate reagents and strategies is crucial for the success of transcriptomic research in archaea.
Article
Biochemistry & Molecular Biology
Caixia Pei, Hua Lu, Jiayin Ma, Jerry Eichler, Ziqiang Guan, Linlu Gao, Li Liu, Hui Zhou, Jinghua Yang, Cheng Jin
Summary: AepG is identified as a dolichol phosphate glucuronosyltransferase involved in the synthesis of acidic exopolysaccharide in H. hispanica, which is vital for the adaptation to high salt environment.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jing Hou, Xue-Meng Yin, Yang Li, Dong Han, Bu Lu, Jia-Yi Zhang, Heng-Lin Cui
Summary: The extracellular protease HlyA from Halococcus salifodinae exhibited good temperature, pH, and salinity tolerance, with significant enhancements in activity by Ca2+, Mg2+, and Sr2+. It was completely inhibited by phenylmethanesulfonyl fluoride (PMSF), indicating it is a serine protease. Additionally, HlyA showed good tolerance to some surfactants and organic solvents, making it a promising candidate for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Maryam Siroosi, Farshad Borjian Borujeni, Mohammad Ali Amoozegar, Hamid Babavalian, Mehdi Hassanshahian
Summary: The study focused on the production and partial purification of amylase from a halophilic archaeon, identifying optimal conditions for enzyme production and activity. A strain of Haloarcula sp. D61 was found to produce amylase under stress conditions with 23% salts, with peak production at 3-4 M NaCl, 40-45°C, and pH 7.0. Different carbon sources affected amylase production, with maltose inducing production while lactose, sucrose, and glucose repressed it.
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Marc Kenneth M. Cajili, Eloise Prieto
Summary: This study investigated the role of archaeal architectural proteins Alba and Cren7 in chromatin folding and dynamics. The results showed that Alba can mediate the formation of folded DNA structures to regulate chromatin compaction, while Cren7 affects the formation of Alba-mediated higher-order chromatin structures.
Article
Food Science & Technology
Wei Wei, Xinyu Hu, Sai Yang, Kaili Wang, Chunlin Zeng, Zhaozhi Hou, Henglin Cui, Shenkui Liu, Lin Zhu
Summary: This study identified the microbial groups responsible for the natural degradation of nitrite in salted radish and elucidated the associated metabolic mechanism. Halophilic archaea from pickling salt dominate the denitrification pathway, with key genera including Haloarcula, Halolamina, and Halobacterium. These archaea cope with high salt stress through a salt-in mechanism and efficiently reduce nitrite to nitrogen, providing important insights for nitrite hazard prevention in high-salinity salted vegetables.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Po-Jung Pao, Min-Feng Hsu, Ming-Hui Chiang, Chun-Ting Chen, Cheng-Chung Lee, Andrew H-J Wang
Summary: Specific antibody interactions with short peptides have led to the development of epitope tagging systems used in various biological research fields. A novel epitope tagging system using the monoclonal antibody GD-26 was developed, recognizing the TD peptide with a 3(10) helix conformation. The strong binding between the TD peptide and GD-26 Fab is attributed to both polar and nonpolar forces.
Article
Genetics & Heredity
Mike Dyall-Smith, Friedhelm Pfeiffer, Pei-Wen Chiang, Sen-Lin Tang
Summary: Hardycor1 virus, isolated in 1998 infecting Halorubrum coriense, has a large genome with low similarity to known viruses. The virus stock also contains two induced proviruses of the host.
Article
Environmental Sciences
Bing-Bing Liu, Rajivgandhi Govindan, Maruthupandy Muthuchamy, Shuang Cheng, Xuebin Li, Lijing Ye, Lai-you Wang, Shu-xian Guo, Wen-Jun Li, Naiyf S. Alharbi, Jamal M. Khaled, Shine Kadaikunnan
Summary: In this study, 75 halophilic archaeal strains were isolated from Wuyongbulake salt lake sediment sample using phenol enrichment method. The majority of the identified strains were phenol-tolerant. The suspension of strains 869-1 and 121-1 exhibited the highest phenol degradation rate, while the supernatant of strain 630-3 showed the highest rate in a simulated artificial wastewater experiment. The EPS produced by strains A00135, 558-1, 869-1, 121-1, and A387 showed excellent phenol removal ability.
Article
Chemistry, Inorganic & Nuclear
Deeb Taher, Firas F. Awwadi, Mousa Al-Noaimi, Lina K. Khader, Hassan K. Juwhari, Hazem Amarne, Mohammed H. Kailani, Abdellatif Ibdah
INORGANICA CHIMICA ACTA
(2019)
Article
Materials Science, Multidisciplinary
Ammar Mahmoud Al-Husseini, Bashar Lahlouh
BULLETIN OF MATERIALS SCIENCE
(2019)
Article
Engineering, Electrical & Electronic
Shadia J. Ikhmayies, Sufian Abedrabbo, Bashar Lahlouh
JOURNAL OF ELECTRONIC MATERIALS
(2019)
Article
Nanoscience & Nanotechnology
Bashar Lahlouh, Ammar Al-Husseini, Aminah Eniyazi
APPLIED NANOSCIENCE
(2020)
Article
Environmental Sciences
Othman Al-Mashaqbeh, Diya Alsafadi, Sahar Dalahmeh, Shannon Bartelt-Hunt, Daniel Snow
Article
Materials Science, Multidisciplinary
Ebraheem O. Hawarat, Mousa M. A. Imran, Omar A. Lafi, Hassan K. Juwhari, Bashar Lahlouh, N. Chandel C. N. Mehta
Correction
Environmental Sciences
Othman Al-Mashaqbeh, Diya Alsafadi, Sahar Dalahmeh, Shannon Bartelt-Hunt, Daniel Snow
Article
Chemistry, Multidisciplinary
Zakariyya Ishtaiwi, Deeb Taher, Marcus Korb, Wissam Helal, Afnan Al-Hunaiti, Hassan K. Juwhari, Hazem Amarne, Mohammad W. Amer, Yaser A. YouSef, Sami Klaib, Sultan Abu-Orabi
Summary: The electronic effects of electron donating and withdrawing groups on the properties of N-(4-R-phenyl)-N'-(4-nitrophenyl)oxamito zincate(II) complexes were investigated. The synthesis, spectroscopic characterization, crystal structure, and luminescent properties of the complexes were studied. The results show that the complexes exhibit stable and unique optical properties.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Physical
Randa M. Al-As'ad, Abdel-Aziz Abu-Yamin, Marcus Korb, Khaled Al Khalyfeh, Ismael A. Elayan, Mansour H. Almatarneh, Hassan K. Juwhari, Hazem Amarne, Deeb Taher, Zakariyya Ishtaiwi, Heinrich Lang
Summary: The luminescent title complex [Dy(NO3)3L3(H2O)].C2H5OH; C34H38DyN5O17 ( L = ethyl (E)-4-((2-hydroxybenzylidene)amino)benzoate) (1) was prepared and characterized using single-crystal X-ray diffraction, FTIR, UV-vis, and PL. The Schiff-base ligands are in a zwitterionic form and coordinate through the phenolic oxygen only. The complex exhibits a triclinic crystal structure and shows potential as a light emitting material in the visible region.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Materials Science, Composites
Ahmed Ali Husein Qwasmeh, Batool A. A. Abu Saleh, Mohammed Al-Tweissi, Mou'ad A. Tarawneh, Ziad M. M. Elimat, Ruba I. I. Alzubi, Hassan K. K. Juwhari
Summary: This study investigated the impact of gamma irradiation on the optical properties of polymer electrolyte thin films composed of poly(ethylene oxide) (PEO) doped with different concentrations of potassium iodide (KI) salt. The optical absorption spectrum of each film was measured using a UV-Vis spectrophotometer. The results showed that increasing the KI concentration and gamma irradiation dose influenced the properties, such as energy gap, absorption coefficient, extinction coefficient, refractive index, and dielectric constant of the PEO electrolyte. These findings have potential applications in optimizing polymers for optical devices and energy storage systems.
JOURNAL OF COMPOSITES SCIENCE
(2023)
Article
Materials Science, Composites
Batool A. A. Abusaleh, Ziad M. M. Elimat, Ruba I. I. Alzubi, Hassan K. K. Juwhari
Summary: This study investigates the changes in the AC electrical properties of epoxy nano-silica composite sheets after exposure to gamma radiation. The results show that gamma irradiation enhances the AC electrical conductivity and dielectric constant values of the nano-silica composites due to the release of more free electrons. The AC electrical properties of the epoxy nano-silica composites were studied and discussed before and after gamma radiation with different dosages, and were found to marginally increase with increasing doses of gamma irradiation.
JOURNAL OF COMPOSITES SCIENCE
(2023)
Article
Chemistry, Physical
Zakariyya Ishtaiwi, Deeb Taher, Marcus Korb, Wissam Helal, Hassan K. Juwhari, Afnan Al-Hunaiti, Hazem Amarne, Khaleel Assaf, Lubna Alrawashdeh, Mohammad W. Amer, Yaser A. Yousef, Heinrich Lang
Summary: A series of N-(3-NO2-C6H4)-N'-(4-R-C6H4)oxamato zincate(II) complexes were synthesized to study the influence of different electron-withdrawing and electron-donating groups R on their electronic properties. The crystal structures of 3c and 3f were determined, confirming the tetrahedral environment around Zn(II) set by the oxamato ligands. UV-Vis spectra and TD-DFT calculations confirmed the presence of LLCT (π -> π*) bands in the complexes, indicating their potential as luminescent materials.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Engineering, Chemical
Othman A. Al-Mashaqbeh, Ayoup M. Ghrair, Diya Alsafadi, Sahar S. Dalahmeh, Shannon L. Bartelt-Hunt, Daniel D. Snow
DESALINATION AND WATER TREATMENT
(2020)
Proceedings Paper
Materials Science, Multidisciplinary
Shadia J. Ikhmayies, Hassan K. Juwhari, Bashar Lahlouh
CHARACTERIZATION OF MINERALS, METALS, AND MATERIALS 2019
(2019)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
