Review
Immunology
Charneal L. Dixon, Katrina Mekhail, Gregory D. Fairn
Summary: Phagocytosis is a process used by cells to engulf particles, with proteins modified by lipids to regulate signal transduction and immune functions. S-acylation, specifically S-palmitoylation, is a reversible modification that plays a role in regulating phagocytosis and phagosome biology in macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Soumya Kanti Gosh, Divanshu Shukla, Hima Mahour, Sunit Kumar Srivastava, Neelam Bodhale, Raja Banerjee, Bhaskar Saha
Summary: This study revealed that Leishmania parasite regulates TLR2 signaling through TPL2 when elicited by the interaction between surface lipophosphoglycan (LPG) and TLR2. Blocking TLR2 or TPL2 can differentially influence TLR2 signaling pathway and modulate the expression of inflammatory cytokines and pathogen activation in macrophages.
Article
Microbiology
Marie-Michele Guay-Vincent, Christine Matte, Anne-Marie Berthiaume, Martin Olivier, Maritza Jaramillo, Albert Descoteaux
Summary: In this study, we found that inhibiting the activity of GP63 during the processing of macrophages prevented the degradation of previously identified GP63 substrates. This highlights the importance of efficiently inhibiting GP63 activity during the preparation of Leishmania-infected host cell lysates.
Article
Immunology
Alonso da Silva Lira Filho, Emanuella Francisco Fajardo, Kwang Poo Chang, Pauline Clement, Martin Olivier
Summary: This study focuses on the role of GP63 in Leishmania spp.-derived exosomes/extracellular vesicles (EVs/LeishEXO) in modulating immune responses and exacerbating cutaneous leishmaniasis. The researchers confirmed the specificity of a novel model by analyzing the protein composition of the extracted EVs and found that GP63 was the primary virulence factor altered in the composition. They also demonstrated that EVs with different levels of GP63 cargo had distinct immunomodulatory capabilities and impact on the cutaneous pathology of Leishmania spp. infection. These findings provide new insights into the immune response and host-pathogen interaction in cutaneous leishmaniasis and have implications for the development of new treatments and vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Emily Naish, Alexander J. T. Wood, Andrew P. Stewart, Matthew Routledge, Andrew Conway Morris, Edwin R. Chilvers, Katharine M. Lodge
Summary: Neutrophils, the most abundant circulating leukocytes, play a crucial role in the initial innate immune response to infection. They eliminate pathogens through phagocytosis and a complex interplay of multiple systems and mechanisms within the phagosome creates an antimicrobial environment. However, gaps in knowledge and controversies exist regarding how neutrophil phagosomes optimize pathogen killing.
IMMUNOLOGICAL REVIEWS
(2023)
Review
Cell Biology
Johannes Westman, Sergio Grinstein
Summary: The ability of phagosomes to halt microbial growth is closely linked to their ability to acidify their luminal pH, but certain pathogens can survive and replicate inside phagosomes by targeting the pH-regulatory machinery of host cells to survive or escape.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Hamlet Acevedo Ospina, Marie-Michele Guay-Vincent, Albert Descoteaux
Summary: Leishmania parasites colonize host phagocytes by altering host cell metabolism and mitochondrial biogenesis, through the action of lipophosphoglycan and type I interferon receptor signaling. These changes create a metabolically adapted environment conducive to parasite replication.
Article
Immunology
Graziele Quintela-Carvalho, Astrid Madeleine Calero Goicochea, Vanessa Mancur-Santos, Sayonara de Melo Viana, Yasmin da Silva Luz, Beatriz Rocha Simoes Dias, Milena Lazaro-Souza, Martha Suarez, Camila Indiani de Oliveira, Elvira M. Saraiva, Claudia Brodskyn, Patricia T. Veras, Juliana P. B. de Menezes, Bruno B. Andrade, Jonilson Berlink Lima, Albert Descoteaux, Valeria M. Borges
Summary: Lipophosphoglycan (LPG) may play a critical role in promoting Leishmania survival in human neutrophils through cellular activation and generation of free radicals, thereby influencing the process of infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Somenath Banerjee, Dipayan Bose, Subhadip Das, Nabanita Chatterjee, Snehasish Mishra, Krishna Das Saha
Summary: In this study, the researchers investigated the generation and function of lipid droplets (LD) in Leishmania donovani infection. They found that infection with L. donovani triggers LD formation in macrophages in a time-dependent manner. The signaling molecules responsible for LD accumulation were also identified. Moreover, inhibition of phagosome maturation enhanced LD accumulation in certain strains of Leishmania. Treatment with aspirin not only reduced LD load but also improved phagolysosome biogenesis and cytokine balance. These findings suggest that manipulating LD generation could be a potential therapeutic strategy against parasite growth in the early stages of infection.
MOLECULAR IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Zhaolun Li, Yulu Gao, Gang Chen, Leili Wang, Jiaqi Chu, Hongyu Han, Yuting Chen, Guang Zhou
Summary: The immune system is a complex mechanism that has evolved to protect people from infectious pathogens. Phagocytosis plays a crucial role in both innate and acquired immunity, allowing for the consumption and elimination of microbial pathogens and apoptotic cells. However, some pathogens have developed strategies to evade detection and killing by phagocytosis. Understanding immune evasion is important in the face of increasing infectious diseases and antibiotic resistance, as it can lead to the development of new treatments and vaccines. Additionally, researchers often focus on the immune evasion of individual pathogens without summarizing the overall mechanisms from an immune perspective.
CELLULAR AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Felipe D. Rego, Camila D. A. Cardoso, Paulo Otavio L. Moreira, Paula M. Nogueira, Marcio S. Araujo, Valeria Matos Borges, Marcia D. Laurenti, Daniella C. Bartholomeu, Alexandre B. Reis, Rubens L. D. Monte-Neto, Rodrigo P. Soares
Summary: LPG, the major glycoconjugate of Leishmania, induces pro-inflammatory/immunosuppressive innate immune responses. This study evaluated the functional/biochemical properties of LPG from different strains of Leishmania amazonensis and found that they have different pro-inflammatory profiles and susceptibility to antileishmanial agents.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Aretha Chan, Jose-Mauricio Ayala, Fernando Alvarez, Ciriaco Piccirillo, George Dong, David Langlais, Martin Olivier
Summary: This study analyzed the early innate inflammatory events during L. major infection, confirming the significant role of the virulence factor GP63 in providing favorable conditions for host cell infection. The data suggest that GP63 does not greatly influence pathogen-induced inflammatory cell recruitment, but rather affects their activation status and effector function, as well as potentially influencing the internalization of promastigotes during early infection.
Article
Nanoscience & Nanotechnology
Wenhao Cheng, Masahiro Fukuda, Sundol Kim, Yuan Liu, Xingchi Chen, Christina Holmes, Yan Li, Hoyong Chung, Yi Ren, Jingjiao Guan
Summary: This study presents a robust engineering method for rupturing phagosomes based on a well-defined mechanism. The method uses microfabricated microparticles composed of uncrosslinked linear poly-(N-isopropylacrylamide) (PNIPAM) as phagocytic objects. By exposing the cells to a cold shock, the microparticle-containing phagosomes rupture. The osmotic pressure generated by dissolved microparticles is the probable cause of phagosomal rupture, as supported by modeling and experimental results.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Roni Levin-Konigsberg, Adriana R. Mantegazza
Summary: Phagocytosis is a key mechanism for immunity and homeostasis, involving the formation of specialized compartments called phagosomes. Phagosome maturation is associated with signaling pathways that link phagocytosis to antigen presentation and immune responses, while phagosome resolution involves the metabolism of degraded cargo and recycling of vesicles. Overall, phagosome maturation and resolution encompass dynamic events and organelle crosstalk that play crucial roles in cellular mechanisms and immune responses.
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Hematology
R. C. Hider, A. M. N. Silva, M. Podinovskaia, Yongmin Ma
COOLEY'S ANEMIA: NINTH SYMPOSIUM
(2010)
Article
Biochemistry & Molecular Biology
Yongmin Ma, Maria Podinovskaia, Patricia J. Evans, Giovanni Emma, Ulrich E. Schaible, John Porter, Robert C. Hider
BIOCHEMICAL JOURNAL
(2014)
Article
Cell Biology
Maria Podinovskaia, Wonsik Lee, Shannon Caldwell, David G. Russell
CELLULAR MICROBIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Ana Nunes, Maria Podinovskaia, Andreia Leite, Paula Gameiro, Tao Zhou, Yongmin Ma, Xiaole Kong, Ulrich E. Schaible, Robert C. Hider, Maria Rangel
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2010)
Article
Chemistry, Medicinal
Sarah Fakih, Maria Podinovskaia, Xiaole Kong, Helen L. Collins, Ulrich E. Schaible, Robert C. Hider
JOURNAL OF MEDICINAL CHEMISTRY
(2008)
Article
Chemistry, Medicinal
Sarah Fakih, Maria Podinovskaia, Xiaole Kong, Ulrich E. Schaible, Helen L. Collins, Robert C. Hider
JOURNAL OF PHARMACEUTICAL SCIENCES
(2009)
Article
Microbiology
Matthew Rogers, Pascale Kropf, Beak-San Choi, Rod Dillon, Maria Podinovskaia, Paul Bates, Ingrid Mueller
Article
Biochemistry & Molecular Biology
Yu Wu, Claire Boulogne, Stefan Carle, Maria Podinovskaia, Holger Barth, Anne Spang, Jean-Christophe Cintrat, Daniel Gillet, Julien Barbier
Article
Biology
Maria Podinovskaia, Cristina Prescianotto-Baschong, Dominik P. Buser, Anne Spang
Summary: This study introduces a novel live-cell imaging assay for monitoring endosome maturation process, applicable to various types of mammalian cells. It was found that different endosome conversion processes and acidification relied on different factors. This assay serves as a powerful tool to further unravel various aspects of endosome maturation.
Article
Biology
Evgeniya Nazarova, Maria Podinovskaia, David G. Russell, Brian C. VanderVen
Article
Microbiology
David G. Russell, Wonsik Lee, Shumin Tan, Neelima Sukumar, Maria Podinovskaia, Ruth J. Fahey, Brian C. Vanderven
MICROBIOLOGY SPECTRUM
(2014)
Meeting Abstract
Hematology
B Austen, M Podinovskaia, C Almond, G Fews, A Gardiner, D Oscier, P Moss, T Stankovic
Meeting Abstract
Hematology
B Austen, M Podinovskaia, J Starcynski, G Barone, A Reiman, P Byrd, G Pratt, M Taylor, T Stankovic
Article
Respiratory System
AA Awomoyi, S Nejentsev, A Richardson, J Hull, O Koch, M Podinovskaia, JA Todd, KPWJ McAdam, JM Blackwell, D Kwiatkowski, MJ Newport
