Protective effects of citrus based mixture drinks (CBMDs) on oxidative stress and restraint stress

In the current study investigated the protective effects of citrus based mixture drinks (CBMDs) using oxidative stress in human dermal fibroblast (HDF) cells and restraint-stressed rats. The CBMDs contained citrus bioflavonoids including narirutin and hesperidin. The cell viability of HDF cells treated with H2O2 was observed at 53.9% but treated with CBMD-1 and CBMD-2 (500g/mL) on H2O2 exposed HDF cells significantly increased the relative cell viability at 65.0 and 72.2%, respectively. In the treadmill test, the time spent on the electrode plate in the restraint-stressed group was analyzed 24.1s, but restraint-stressed rats with administered CBMDs (300mg/kg) had significantly decreased the time at 2.4 (CBMD-1) and 4.7 (CBMD-2)s, respectively. In addition, number of touches the electrode plate in restraint-stressed group was observed at 42.4ea, but, restraint-stressed rats with administered CBMD-1 and CBMD-2 (300mg/kg) were significantly decreased at 7.0 and 10.2ea, respectively.