期刊
FOOD CHEMISTRY
卷 254, 期 -, 页码 340-347出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.02.051
关键词
In silico proteolysis; Yak milk casein; Angiotensin I-converting enzyme inhibitory; peptide; Bioactive peptides
资金
- Science and Technology Major Project of Gansu Province, China [17ZD2NP025]
- Major Program for the Applied Technology Research and Development Plan of Heilongjiang Province, China [GA16B201-2]
- National Dairy Processing Technology Research Center of China [HL2015-1]
Yak milk casein was selected as a potential precursor of bioactive peptides based on in silico analysis. Most notable among these are the angiotensin I-converting enzyme (ACE) inhibitory peptides. First, yak milk casein has high homology with cow milk casein by homologous analysis. The potential of yak milk casein for the releasing bioactive peptides was evaluated by determining the frequency of occurrence of fragments with a given activity. Through the BIOPEP database analysis, there are many bioactive peptides in yak milk casein sequences. Then, an in silico proteolysis using single or combined enzymes to obtained ACE inhibitory peptides was investigated. Cytotoxicity analysis using the online toxic prediction tool ToxinPred revealed that all in silico proteolysis derived ACE inhibitory peptides are non-cytotoxic. Overall, the present study highlights a in silico proteolysis approach to assist the yak milk casein releasing ACE inhibitory peptides and provides a guidance for the actual hydrolysis of proteins for the production of bioactive peptides.
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