Article
Cell Biology
Moa Stenudd, Hanna Sabelstrom, Enric Llorens-Bobadilla, Margherita Zamboni, Hans Blom, Hjalmar Brismar, Shupei Zhang, Onur Basak, Hans Clevers, Christian Goritz, Fanie Barnabe-Heider, Jonas Frisen
Summary: Spinal cord ependymal cells can possess neural stem cell properties and have the potential to generate scar-forming astrocytes and remyelinating oligodendrocytes after injury. A small subpopulation of these cells with stem cell potential, called ependymal A (EpA) cells, can revert to a stem cell state and serve as a therapeutic target for spinal cord repair.
Review
Cell Biology
Shanshan Chen, Wenqi Wang, Hor-Yue Tan, Yuanjun Lu, Zhiping Li, Yidi Qu, Ning Wang, Di Wang
Summary: Autophagy is crucial for cellular homeostasis and senescence, with dysregulation implicated in various human diseases. This review highlights the role of autophagy in stem cell maintenance, focusing on its interaction with adult stem cells in disease initiation and progression. Understanding the mechanisms of autophagy in maintaining stemness may provide insights into disease pathogenesis and therapy development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Ying Liu, Leilei Jiang, Wenbo Song, Chenxi Wang, Shiting Yu, Juhui Qiao, Xinran Wang, Chenrong Jin, Daqing Zhao, Xueyuan Bai, Peiguang Zhang, Siming Wang, Meichen Liu
Summary: Recent studies have shown that stem cells have notable abilities of proliferation, differentiation, and self-renewal, making them important in regenerative medicine and anti-aging research. Therefore, there is a growing interest in identifying natural medicines that can influence the fate of stem cells. Ginsenosides, the key components of ginseng, have been extensively studied for their effects on stem cell function and aging resistance. This review aims to summarize the latest progress on the impact of ginsenosides on adult stem cell behavior, particularly in terms of proliferation, differentiation, and self-renewal.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xue Feng, Suisui Jiang, Fan Zhang, Runfang Wang, Yuanhui Zhao, Mingyong Zeng
Summary: The study demonstrates that WSMP extracted from oyster shells can promote the proliferation, differentiation, and mineralization of osteoblasts both in vitro and in vivo. This finding suggests a potential application of WSMP in bone regeneration and osteoporosis treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Cheng Jin, Zhipeng Wang, Pengyu Li, Jielin Tang, Tao Jiao, Yiran Li, Jinhuan Ou, Dingfeng Zou, Mengzhen Li, Xinyu Mang, Jun Liu, Yanni Ma, Xiaolong Wu, Jie Shi, Shitao Chen, Manman He, Yan Lu, Ning Zhang, Shiying Miao, Fei Sun, Linfang Wang, Kai Li, Jia Yu, Wei Song
Summary: This study investigates the intercellular interactions between spermatogonial stem cell (SSC) and testicular niche in both adult mice and humans, uncovering the crucial role of C-C motif chemokine ligand 24 and insulin-like growth factor binding protein 7 in maintaining SSC homeostasis. The clinical relevance of specific paracrine factors in human fertility is also established.
Article
Multidisciplinary Sciences
Azzurra Codino, Tomasz Turowski, Louie N. van de Lagemaat, Ivayla Ivanova, Andrea Tavosanis, Christian Much, Tania Auchynnikava, Lina Vasiliauskaite, Marcos Morgan, Juri Rappsilber, Robin C. Allshire, Kamil R. Kranc, David Tollervey, Donal O'Carroll
Summary: The RNA-binding protein NANOS2 plays a crucial role in maintaining spermatogonial stem cells (SSCs) by recognizing specific consensus motifs in the 3' untranslated region of target mRNAs. NANOS2 interacts with the CCR4-NOT deadenylase complex in SSCs, reducing the stability of target transcripts and affecting key self-renewal pathways related to SSC maintenance.
Article
Cell & Tissue Engineering
Shuai Ma, Si Wang, Yanxia Ye, Jie Ren, Ruiqing Chen, Wei Li, Jiaming Li, Liyun Zhao, Qian Zhao, Guoqiang Sun, Ying Jing, Yuesheng Zuo, Muzhao Xiong, Yuanhan Yang, Qiaoran Wang, Jinghui Lei, Shuhui Sun, Xiao Long, Moshi Song, Shuyang Yu, Piu Chan, Jianwei Wang, Qi Zhou, Juan Carlos Izpisua Belmonte, Jing Qu, Weiqi Zhang, Guang-Hui Liu
Summary: This study provides a comprehensive framework to explore aging and rejuvenating trajectories at single-cell resolution, revealing cellular and molecular programs that instruct systemic revitalization by blood-borne factors. Specifically, it identified hematopoietic stem and progenitor cells (HSPCs) as one of the most responsive cell types to young blood exposure, initiating a continuum of cell state changes and restoring a youthful transcriptional regulatory program and cell-cell communications in HSPCs.
Review
Pharmacology & Pharmacy
Xiaomeng Guo, Ruishuang Ma, Meng Wang, Benson Wui-Man Lau, Xiaopeng Chen, Yue Li
Summary: This review discusses the impact and mechanisms of CTS on various stem cells, as well as its involvement in the processes of stem cell behavior, providing new insights for the treatment of high-incidence diseases.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Shinnosuke Suzuki, John R. McCarrey, Brian P. Hermann
Summary: Spermatogonial stem cells maintain spermatogenesis by balancing self-renewal and differentiation, with mTORC1 signaling playing a crucial role in the process.
Review
Cell Biology
Jiawen Chen, Tianyu Sun, Yan You, Buling Wu, Xiaofang Wang, Jingyi Wu
Summary: Proteoglycans are essential for regulating stem cell homeostasis and show potential for being utilized as novel glycomics carriers or bio-active molecules in bone regeneration. Recent research has outlined their distinct profiles and functions in maintaining the balance between self-renewal and differentiation of stem cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Anna Altshuler, Aya Amitai-Lange, Noam Tarazi, Sunanda Dey, Lior Strinkovsky, Shira Hadad-Porat, Swarnabh Bhattacharya, Waseem Nasser, Jusuf Imeri, Gil Ben-David, Ghada Abboud-Jarrous, Beatrice Tiosano, Eran Berkowitz, Nathan Karin, Yonatan Savir, Ruby Shalom-Feuerstein
Summary: This study revealed the differences in LSC populations at different locations, maintaining corneal health in the outer and inner limbal regions. It also found that LSC populations are abundant, following stochastic rules and neutral drift dynamics.
Article
Immunology
Michael Solomon, Baobao Song, Vinothini Govindarajah, Samantha Good, Ashok Arasu, E. Broderick Hinton, Kairavee Thakkar, James Bartram, Marie-Dominique Filippi, Jose A. Cancelas, Nathan Salomonis, H. Leighton Grimes, Damien Reynaud
Summary: Using CITE-seq analysis, a distinct progenitor population with a cell cycle gene signature similar to quiescent hematopoietic stem cells was identified. This population can be phenotypically enriched using the CD62L marker in the Flt3(+) multipotent progenitor compartment. Functional analysis confirmed the heterogeneity of the progenitor compartment and established the quiescent/slow-cycling properties of CD62L(-) MPP4 cells. Additionally, the study revealed a hierarchical organization of the MPP compartments in native conditions.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Jingjing Hu, Ke Chen, Fangfang Hong, Guosheng Gao, Xiaoyu Dai, Hua Yin
Summary: METTL3 modifies SOCS3 mRNA with m6A to promote the stemness and tumorigenicity of LCSCs.
CANCER GENE THERAPY
(2023)
Review
Cell Biology
Toshihiro Mitaka, Norihisa Ichinohe, Naoki Tanimizu
Summary: Mature hepatocytes in the liver can be categorized into three subpopulations based on their proliferative capability: type I, type II, and type III cells. Small hepatocytes (SHs) are unique cells that appear in the liver and may play a role in liver regeneration. They can be derived from hepatocytic progenitors and are involved in the liver's development and growth.
Article
Cell Biology
Yosuke Fujita, Tomoki Nagakura, Hiroyuki Uchino, Masato Inazu, Tsuyoshi Yamanaka
Summary: The study found that CTL1 is responsible for extracellular choline uptake, while CTL2 may uptake choline in the mitochondria and be involved in DNA methylation via choline oxidation. Inhibition of extracellular choline uptake caused intracellular choline deficiency in hNSCs, suppressing cell proliferation, cell viability, and neurite outgrowth. The findings contribute to understanding the role of choline in neural development and the pathogenesis of neurological diseases caused by choline deficiency or impairment in choline uptake.
Article
Biochemistry & Molecular Biology
Hanieh Hadady, Fereshteh Karamali, Fatemeh Ejeian, Sareh Soroushzadeh, Mohammad Hossein Nasr-Esfahani
Summary: This study evaluated two methods (micro-electromechanical systems and ultrafiltration) for isolating extracellular vesicles (EVs) from stem cells from apical papilla (SCAP). It found that different methods enriched different subsets of EVs, which significantly impacted their biological and functional effects. Further experiments confirmed the neuroprotection and anti-inflammatory capacity of SCAP-EVs in vitro, as well as their potential therapeutic effects in a retinal degeneration model.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Correction
Oncology
Manizheh Sajadi, Mohammad Fazilati, Habibollah Nazem, Mohammad Mahdevar, Kamran Ghaedi
CANCER CELL INTERNATIONAL
(2023)
Article
Biology
M. Zanjirband, M. Baharlooie, Z. Safaeinejad, M. H. Nasr-Esfahani
Summary: This study investigated the novel therapeutics targets in PCOS by analyzing the transcriptome of granulosa cells using a drug repositioning approach. The results could provide valuable insights for the management of PCOS.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Clinical Neurology
Leila Asad Samani, Kamran Ghaedi, Ahmad Majd, Maryam Peymani, Masoud Etemadifar
Summary: This study aimed to assess the overlap of PD patients' transcriptomes in the substantia nigra (SN) with peripheral blood mononuclear cells (PBMCs) to discover potential biomarkers for diagnosis. Through the analysis of gene expression differences and validation using RT-qPCR, HSPA1A/B, DGKH, and NOTCH2 were identified as potential biomarkers for PD diagnosis.
NEUROLOGICAL SCIENCES
(2023)
Article
Pathology
Niloufar Salimian, Maryam Peymani, Kamran Ghaedi, Sepideh Mirzaei, Mehrdad Hashemi
Summary: Recent findings have indicated an increased expression of LINC01929 in various cancer types, accompanied by a higher mortality rate. The gene co-expression network revealed a strong association between LINC01929 and metastatic genes such as COL5A1. RT-qPCR confirmed the elevated expression of LINC01929 in colorectal, gastric, and breast cancer tissues. LINC01929 could serve as a potential candidate for diagnosis and therapy as an oncogene and regulator of metastatic pathways.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Multidisciplinary Sciences
Marjan Sadeghi, Mohsen Rahimi Andani, Mehdi Hajian, Nafiseh Sanei, Reza Moradi-Hajidavaloo, Nasrin Mahvash, Farnoosh Jafarpour, Mohammad Hossein Nasr-Esfahani
Summary: The role of the WNT/beta-catenin signaling pathway in preimplantation goat development was investigated. The expression of beta-CATENIN was observed in IVF and SCNT embryos, and inhibition of beta-catenin resulted in increased blastocyst formation rate. Different localization patterns of beta-catenin were observed in IVF and SCNT blastocysts. Inhibition of WNT signaling during the compact morula to blastocyst transition improved preimplantation embryonic development.
Article
Obstetrics & Gynecology
Aram Minas, Mazdak Razi, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Hashemi-Asl, Marziyeh Tavalaee
Summary: Varicocele (VCL) induces severe oxidative stress in testicular tissue, leading to infertility in 35% of males. This study compares the impact of varicose vessels on oxidative DNA damage and antioxidant reactions in the seminiferous tubules. The results show that varicocele causes significant DNA damage, reduces antioxidant reactions, and negatively affects the cell cycle progression.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maryam Mirani, Soghra Bahmanpour, Fatemeh Masjedi, Zahra Derakhshan, Mahintaj Dara, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Bagher Tabei
Summary: This study reveals the negative effects of AGEs-RAGE axis on steroidogenesis in luteinized GCs, which can be mitigated by PM treatment. Understanding the interaction between AGEs and PM in ovarian physiology and pathology may lead to more targeted therapy for ovarian dysfunction.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Psychology, Biological
Fatemeh Kazeminasab, Maryam Baharlooie, Hossein Rezazadeh, Nepton Soltani, Sara K. Rosenkranz
Summary: This study evaluated the effects of aerobic exercise on glucose metabolism and lipid profiles in healthy, prediabetic, and T2DM mice. The results showed that aerobic exercise can increase the expression of key genes, improve insulin resistance, and decrease the expression of genes related to gluconeogenesis in the liver.
PHYSIOLOGY & BEHAVIOR
(2023)
Article
Multidisciplinary Sciences
Ali Moghimi-Khorasgani, Farshad Homayouni Moghadam, Mohammad Hossein Nasr-Esfahani
Summary: Microglial cells (MGCs) are resident macrophages in the brain and spinal cord, playing a crucial role in immune defense and transition between different states. Increased Nurr1 expression is associated with anti-neuroinflammatory responses. This study investigates the potential role of ferulic acid (FA) in facilitating microglia transition by inducing Nurr1 expression.
Review
Biochemistry & Molecular Biology
Khatere Mokhtari, Maryam Peymani, Mohsen Rashidi, Kiavash Hushmandi, Kamran Ghaedi, Afshin Taheriazam, Mehrdad Hashemi
Summary: Methodological innovations over the last four decades have revolutionized transcriptome profiling, with RNA sequencing (RNA-seq) now enabling the sequencing and quantification of transcriptional outputs of individual cells or thousands of samples. This technology allows for a better understanding of the molecular mechanisms underlying cellular behaviors, particularly in the context of cancer, providing opportunities for unraveling tumor complexity and identifying novel biomarkers and treatment options. The transcriptome of colon cancer has been extensively studied in this review, considering various risk factors and non-coding RNAs, and holds promise for earlier and more accurate diagnosis, offering improved prognostic utility to medical teams and patients.
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2023)
Article
Obstetrics & Gynecology
Saeideh Abdollahzadeh, Ahmad Riasi, Marziyeh Tavalaee, Farnoosh Jafarpour, M. H. Nasr-Esfahani
Summary: An imbalance between omega-6 and omega-3 fatty acids in sperm is correlated with lipid peroxidation and DNA damage in sperm, indicating a possible link to fertility potential. This study found that men with high DNA fragmentation had higher levels of saturated, monounsaturated, and omega-6 to omega-3 fatty acid ratios in sperm and seminal plasma compared to men with low DNA fragmentation. Additionally, there were positive associations between the omega-6 to omega-3 fatty acid ratio and sperm lipid peroxidation, DNA fragmentation, and residual histones in both sperm and seminal plasma.
REPRODUCTIVE SCIENCES
(2023)
Article
Multidisciplinary Sciences
M. Zanjirband, R. Hodayi, Z. Safaeinejad, M. H. Nasr-Esfahani, R. Ghaedi-Heydari
Summary: This study investigated the impact of the p53 pathway in enhancing apoptosis and abnormal function of granulosa cells. Results suggest that p53 target genes play a critical role in the pathogenesis of PCOS, specifically through the enhancement of apoptosis in granulosa cells.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Behrouz Aghajanloo, Fatemeh Ejeian, Francesca Frascella, Simone L. L. Marasso, Matteo Cocuzza, Alireza Fadaei Tehrani, Mohammad Hossein Nasr Esfahani, David W. W. Inglis
Summary: Deterministic lateral displacement (DLD) is a passive separation method for particle and cell sorting. It offers high size resolution and operates using capillary pressure, making it suitable for point-of-care devices. The method achieves high separation efficiency and is simple to use, making it a valuable technique in the field of microfluidic technologies.
Review
Nutrition & Dietetics
Fatemeh Kazeminasab, Maryam Baharlooie, Bahareh Karimi, Khatereh Mokhtari, Sara K. Rosenkranz, Heitor O. Santos
Summary: This study aimed to determine whether combining IF with exercise interventions is more favorable in improving cardiometabolic health outcomes than a control diet plus exercise.
Article
Chemistry, Medicinal
Shuang Mei, Su Jiang, Yuting Wang, Han Jing, Peng Yang, Miao-Miao Niu, Jindong Li, Kai Yuan, Yan Zhang
Summary: This study identifies a dual-targeting peptide, AP-1, that effectively inhibits variants of concern (VOCs) of SARS-CoV-2 without impairing host cell viability. The findings suggest that AP-1 could be a promising broad-spectrum agent for treating emerging VOCs of SARS-CoV-2.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Hyeonjun Lee, Ju Yeon Lee, Hyunsoo Jang, Hye Young Cho, Minhee Kang, Sang Hyun Bae, Suin Kim, Eunji Kim, Jaebong Jang, Jin Young Kim, Young Ho Jeon
Summary: By using liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance experiments, we identified new chemical moieties that bind to the target sites of the protein of interest, allowing for reversible binding and protein degradation. This method has the potential to expand the application of PROTAC technology.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Yingying Li, Xiyou Du, Xinru Kong, Yuelin Fang, Zhijing He, Dongzhu Liu, Hang Wu, Jianbo Ji, Xiaoye Yang, Lei Ye, Guangxi Zhai
Summary: This study proposes a novel nanoplatform based on the autophagy cascade to overcome the obstacles in chemo-immunotherapy. The platform combines chemotherapy and starvation therapy to initiate pro-death autophagy and enhance antigen presentation, while also remodeling the immunosuppressive tumor microenvironment. Furthermore, the study discovers a new therapeutic direction for the respiration inhibitor 3-bromopyruvic acid (3BP) in cancer treatment. Overall, this study offers an opportunity to improve antitumor efficacy and boost immune responses.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Bingsi Wang, Mingxu Ma, Yusen Dai, Pengfei Yu, Liang Ye, Wenyan Wang, Chunjie Sha, Huijie Yang, Yingjie Yang, Yunjing Zhu, Lin Dong, Shujuan Wei, Linlin Wang, Jingwei Tian, Hongbo Wang
Summary: Breast cancer is a common malignant tumor in women, and drug resistance remains a clinical challenge. In this study, a novel compound, G-5b, was developed with potent antagonistic and degradation activities comparable to the current drug fulvestrant. G-5b also showed improved stability and solubility. Mechanistically, G-5b engages the proteasome pathway to degrade ER, inhibiting the ER signaling pathway and inducing apoptosis and cell cycle arrest. In animal models, G-5b exhibited superior pharmacokinetics and pharmacodynamics properties. Overall, G-5b is a promising long-acting SERD worthy of further investigation and optimization.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Karoline B. Waitman, Larissa C. de Almeida, Marina C. Primi, Jorge A. E. G. Carlos, Claudia Ruiz, Thales Kronenberger, Stefan Laufer, Marcia Ines Goettert, Antti Poso, Sandra V. Vassiliades, Vinicius A. M. de Souza, Monica F. Z. J. Toledo, Neuza M. A. Hassimotto, Michael D. Cameron, Thomas D. Bannister, Leticia Costa-Lotufo, Joa o A. Machado-Neto, Mauricio T. Tavares, Roberto Parise-Filho
Summary: A series of hybrid inhibitors combining pharmacophores of known kinase inhibitors and benzohydroxamate HDAC inhibitors were synthesized and evaluated for their anticancer activity and pharmacokinetic properties. Compounds 4d-f exhibited promising cytotoxicity against hematological cells and moderate activity against solid tumor models. Compound 4d showed potent inhibition of multiple kinase targets and had stable interactions with HDAC and members of the JAK family. These compounds showed selective cytotoxicity with minimal effects on non-tumorigenic cells and favorable pharmacokinetic profiles.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Michal Sulik, Diana Fontinha, Dietmar Steverding, Szymon Sobczak, Michal Antoszczak, Miguel Prudencio, Adam Huczynski
Summary: This study describes the synthesis of the first-in-class ivermectin derivatives obtained through derivatization of the C13 position, along with the unexpected rearrangement of the macrolide ring. These derivatives show potential for antiparasitic activity and are important for the development of new antiparasitic agents.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Jun Liu, Qiu-Xian Chen, Wen-Fu Wu, Dong Wang, Si -Yu Zhao, Jia-Hao Li, Yi-Qun Chang, Shao-Gao Zeng, Jia-Yi Hu, Yu-Jie Li, Jia-Xin Du, Shu-Meng Jiao, Hai-Chuan Xiao, Qiang Zhang, Jun Xu, Jian-Fu Zhao, Hai -Bo Zhou, Yong-Heng Wang, Jian Zou, Ping-Hua Sun
Summary: A new anti-infective drug strategy has been discovered to attenuate virulence and modulate inflammation caused by drug-resistant Pseudomonas aeruginosa infections. Compound 5f inhibits biofilm formation, macrophage migration, and inflammatory response induced by P. aeruginosa, showing potential as a novel candidate against drug-resistant infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Liuzeng Chen, Ke Wang, Lingyun Wang, Wei Wang, Lifan Wang, Jia Li, Xiaohan Liu, Mengya Wang, Banfeng Ruan
Summary: In this study, a series of novel anti-inflammatory compounds were designed and synthesized based on the natural product pterostilbene skeleton. Among them, compound 8 showed the highest activity and exhibited its effects through inhibition of pro-inflammatory cytokines by blocking the NF-KB/MAPK signaling pathway. Compound 8 also demonstrated a good relieving effect on acute colitis in mice and showed good safety in acute toxicity experiments.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Article
Chemistry, Medicinal
Si-Min Liang, Gui-Bin Liang, Hui-Ling Wang, Hong Jiang, Xian-Li Ma, Jian-Hua Wei, Ri-Zhen Huang, Ye Zhang
Summary: A series of novel multi-target antitumor agents were designed, synthesized, and evaluated. Some compounds exhibited significant antitumor activity and one compound showed excellent efficacy, limited toxicity, and low resistance. Further mechanism studies revealed that the compound exerted antitumor effects through multiple pathways.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
