期刊
FOOD RESEARCH INTERNATIONAL
卷 76, 期 -, 页码 518-526出版社
ELSEVIER
DOI: 10.1016/j.foodres.2015.06.025
关键词
Hydrophobicity; Bioavailability; Antioxidant activity; Gastrointestinal digestion; Caco-2 cell model
资金
- Natural Science Foundation of China [31271846, 31401620]
- Program for New Century Excellent Talents in University of the Ministry of Education of China [2010JS078]
- Funds for Distinguished Young Scientists of Anhui Academy of Agricultural Sciences [14B1211]
- Natural Science Foundation of Anhui Province [1508085QC54]
Alcalase-treated casein hydrolysates were separated into three hydrophobic chromatography fractions (HC-F1, HC-F2 and HC-F3) using YMC ODS C18 column. Simulated gastrointestinal (GI) digestion (stage I digestion) and Caco-2 cell absorption (stage II digestion) were sequentially applied for producing corresponding digests and adsorbates of collected peptide fractions. ANS probe and reverse-phase (RP)-HPLC were applied to detect peptide hydrophobicity, whereas trolox equivalent antioxidant capacity and oxygen radical antioxidant capacity were used to determine their antioxidant activity. Peptide nitrogen was used to evaluate bioavailability (BA) and remaining peptide content. In addition, RP-HPLC and electrospray ionization mass spectrometry were performed to obtain peptide sequences. Results showed that high hydrophobic peptide fractions (HC-F3) had excellent BA and remaining antioxidant activity, but poor digestive stability in stage I digestion. Two peptides, NTVP and IV, were identified from the adsorbate of HC-F3 to be GI-resistant peptides. Therefore, utilizing high hydrophobic peptides from casein as potential functional foods or nutraceuticals was feasible. (C) 2015 Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据