期刊
FOOD CHEMISTRY
卷 173, 期 -, 页码 210-217出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.10.030
关键词
Zinc; Rapeseed protein hydrolysates; Chelating peptides; Immobilized metal affinity chromatography; Alcalase
资金
- Natural Science Foundation of China [31401620]
- Funds for Distinguished Young Scientists of Anhui Academy of Agricultural Sciences [14B1211]
- Twelfth Five-Year Plan for Science & Technology Support Program Project in China [2012BAD14B13]
Zinc is an essential trace element for human growth and development. In this work, zinc-chelating peptides from rapeseed protein hydrolysates produced with alcalase were investigated by affinity chromatography with immobilized zinc and Sephadex G-25 gel filtration. Four small peptides, namely, Ala-Arg, Asn-Ser-Met (NSM), Gly-Lys-Arg, and Glu-Pro-Ser-His, were obtained and identified by reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry. The zinc-chelating ability of the four peptides was further validated by inductively coupled plasma atomic emission spectrometry (ICP-AES). NSM was found to exhibit the highest zinc-chelating rate, which was better than that of reduced glutathione. We speculated that the Asn residue at the amino-terminus might facilitate this zinc-chelating ability. Therefore, utilizing small peptides from rapeseed protein as novel carriers for zinc supplement was feasible. (C) 2014 Elsevier Ltd. All rights reserved.
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