期刊
CHEMICAL SOCIETY REVIEWS
卷 47, 期 7, 页码 2454-2484出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cs00404d
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资金
- US National Institutes of Health [GM122567, NS103573]
- EU FP7 program [ERC-2013-ADG-340233]
- Academy of Finland [263371, 266992]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R35GM122567] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [U01NS103573] Funding Source: NIH RePORTER
Protein kinases are involved in the regulation of many cellular processes including cell differentiation, survival, migration, axon guidance and neuronal plasticity. A growing set of optogenetic tools, termed opto-kinases, allows activation and inhibition of different protein kinases with light. The optogenetic regulation enables fast, reversible and non-invasive manipulation of protein kinase activities, complementing traditional methods, such as treatment with growth factors, protein kinase inhibitors or chemical dimerizers. In this review, we summarize the properties of the existing optogenetic tools for controlling tyrosine kinases and serine-threonine kinases. We discuss how the opto-kinases can be applied for studies of spatial and temporal aspects of protein kinase signaling in cells and organisms. We compare approaches for chemical and optogenetic regulation of protein kinase activity and present guidelines for selection of opto-kinases and equipment to control them with light. We also describe strategies to engineer novel opto-kinases on the basis of various photoreceptors.
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