Article
Cell Biology
Elisabeth Naschberger, Christian Flierl, Jinghao Huang, Lena Erkert, Reyes Gamez-Belmonte, Miguel Gonzalez-Acera, Magdalena Bober, Martin Mehnert, Christoph Becker, Vera S. Schellerer, Nathalie Britzen-Laurent, Michael Stuerzl
Summary: The development of inflammatory bowel diseases (IBD) involves the breakdown of two barriers: the epithelial barrier and the gut-vascular barrier (GVB). Interferon (IFN)-? is a major pathogenesis factor in IBD and can impair both barriers. Secreted factors from the GVB and their impact on communication between the barriers in IBD are discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Julian Heuberger, Jakob Trimpert, Daria Vladimirova, Christian Goosmann, Manqiang Lin, Rosa Schmuck, Hans-Joachim Mollenkopf, Volker Brinkmann, Frank Tacke, Nikolaus Osterrieder, Michael Sigal
Summary: The study suggests that elevated IFN-gamma in COVID-19 can promote differentiation of intestinal epithelial cells into mature enterocytes expressing high levels of ACE2, increasing susceptibility to SARS-CoV-2 infection.
EMBO MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Xiaolei Wang, Terrence Tsz-Tai Yuen, Ying Dou, Jingchu Hu, Renhao Li, Zheng Zeng, Xuansheng Lin, Huarui Gong, Celia Hoi-Ching Chan, Chaemin Yoon, Huiping Shuai, Deborah Tip-Yin Ho, Ivan Fan-Ngai Hung, Bao-Zhong Zhang, Hin Chu, Jian-Dong Huang
Summary: Researchers demonstrate the importance of cellular immunity in protecting against SARS-CoV-2 by showing that mice lacking B cells can still develop a robust immune response through vaccination or viral challenge. This study has important implications for understanding the impact of SARS-CoV-2 variants on the immune system.
NATURE COMMUNICATIONS
(2023)
Article
Agriculture, Dairy & Animal Science
Huaiyong Zhang, Maryam Majdeddin, Djoere Gaublomme, Bernard Taminiau, Matthieu Boone, Dirk Elewaut, George Daube, Ivan Josipovic, Keying Zhang, Joris Michiels
Summary: The study found that heat stress had detrimental effects on intestinal integrity and bone health in broilers, while dietary supplementation of 25-OH-D3 was identified as a potential therapy to prevent these adverse effects.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Mingxing Su, Huixiu Qi, Qingzhen Huang, Lili Wang, Xueqi Guo, Qiang Wang
Summary: Inhalation of arsenic can exacerbate the development of bacterial pneumonia, but the exact mechanism remains unclear. Exposure to arsenic or lipopolysaccharide alone produced different effects, while combined exposure increased inflammatory factors and lung damage. The expression of certain proteins involved in epithelial barrier integrity was further disrupted by combined exposure.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Immunology
Lihong Yao, Ying Tang, Junjie Chen, Jiahui Li, Hua Wang, Mei Lu, Lijuan Gao, Fang Liu, Ping Chang, Xingxing Liu, Haixiong Tang
Summary: Cell-cell junctions are crucial for cellular and tissue polarity and integrity maintenance. Dysfunction of airway epithelial barrier is associated with acute lung injury (ALI) pathogenesis. In this study, it was found that phosphatidylinositol 3-kinase delta (PI3K delta) plays a role in the dysregulation of airway epithelial barrier integrity in ALI. Pharmacological inhibitors of PI3K delta attenuated LPS-induced lung injury and inflammation, restored epithelial cell junction protein expression, and reduced total protein concentration and cytokine secretion in BALF.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lokman Pang, Jennifer Huynh, Mariah G. Alorro, Xia Li, Matthias Ernst, Ashwini L. Chand
Summary: Reduced STAT3 activity in mice leads to increased susceptibility to DSS-induced colitis, indicating that the IL-6/gp130/STAT3 signaling pathway orchestrates intestinal barrier function by modulating cytokine secretion and promoting epithelial integrity to maintain defense against bacteria. Therapeutically targeting this signaling axis may serve as a treatment strategy for IBD patients.
Review
Medicine, General & Internal
Michael Stuerzl, Meik Kunz, Susanne M. Krug, Elisabeth Naschberger
Summary: Inflammatory bowel disease is a chronic inflammatory disorder with a rising incidence. Disease development involves the breakdown of the epithelial cell barrier, where blood vessels play a significant role in disease progression. The communication between the gut-vascular barrier and the epithelial barrier may contribute to the pathogenesis of IBD.
FRONTIERS IN MEDICINE
(2021)
Article
Medicine, General & Internal
Yang Cheng, Tyler R. Hall, Xiao Xu, Ivy Yung, Donald Souza, Jie Zheng, Felix Schiele, Matthias Hoffmann, M. Lamine Mbow, James P. Garnett, Jun Li
Summary: This study reveals that the coordinated upregulation of uPA and uPAR in IBD mucosa is associated with the breakdown of the intestinal epithelial barrier. Inhibiting the interaction between uPA and uPAR through genetic and pharmacological approaches can protect the epithelial barrier. Additionally, uPAR deficiency enhances EGF/EGFR signaling, promoting epithelial homeostasis and repair.
Article
Multidisciplinary Sciences
Keng Ioi Vong, Tsz Ching Ma, Baiying Li, Thomas Chun Ning Leung, Wenyan Nong, Sai Ming Ngai, Jerome Ho Lam Hui, Liwen Jiang, Kin Ming Kwan
Summary: The study reveals that genetic ablation of Sox9 in the choroid plexus results in a hyperpermeable blood-CSF barrier, disrupting CSF electrolyte balance and protein composition. This disruption is mediated by the down-regulation of Col9a3 expression, leading to structural integrity loss of the epithelial basement membrane and perturbed microtubule dynamics necessary for correct tight junction assembly in the CP epithelium.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Medicine, General & Internal
Iaroslav Semin, Justus Ninnemann, Marina Bondareva, Ilia Gimaev, Andrey A. Kruglov
Summary: The microbiota in the intestine plays a crucial role in maintaining the health of the body, but imbalance can lead to inflammation. Epithelial cells act as a barrier to isolate microorganisms, and the nature and duration of stress and inflammation can influence cell responses.
FRONTIERS IN MEDICINE
(2021)
Review
Immunology
Gerasimos Eleftheriotis, Efthymios P. Tsounis, Ioanna Aggeletopoulou, Periklis Dousdampanis, Christos Triantos, Athanasia Mouzaki, Markos Marangos, Stelios F. Assimakopoulos
Summary: COVID-19 can directly infect the gastrointestinal tract or indirectly affect it through viremia and inflammatory mediators, leading to a compromised gut immunological barrier. This barrier dysfunction allows for the translocation of microbes and toxins, triggering a strong systemic immune response. The dysbiotic gut microbiota and proinflammatory environment further contribute to the disruption of the intestinal epithelium. This review highlights the changes in the gut immunological barrier during SARS-CoV-2 infection and their prognostic implications.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Luz del Carmen Martinez-Sanchez, Phuong Anh Ngo, Rashmita Pradhan, Lukas-Sebastian Becker, David Boehringer, Despina Soteriou, Marketa Kubankova, Christine Schweitzer, Tatyana Koch, Veronika Thonn, Lena Erkert, Iris Stolzer, Claudia Guenther, Christoph Becker, Benno Weigmann, Monika Klewer, Christoph Daniel, Kerstin Amann, Stefan Tenzer, Raja Atreya, Martin Bergo, Cord Brakebusch, Alastair J. M. Watson, Jochen Guck, Ben Fabry, Imke Atreya, Markus F. Neurath, Rocio Lopez-Posadas
Summary: Abnormal cell shedding is associated with intestinal barrier dysfunction and inflammatory bowel diseases. Deletion of PGGTase and RAC1 in intestinal epithelial cells leads to cell overcrowding and epithelial leakage, resulting in chronic intestinal inflammation. RAC1 plays a crucial role in regulating cytoskeletal dynamics, cell mechanics, and intestinal cell shedding.
Article
Agriculture, Dairy & Animal Science
Malgorzata Gierynska, Lidia Szulc-Dabrowska, Justyna Struzik, Matylda Barbara Mielcarska, Karolina Paulina Gregorczyk-Zboroch
Summary: This article discusses the importance of the gastrointestinal tract and the key mechanisms of communication between the gut microbiota and epithelial cells in maintaining intestinal homeostasis. The article emphasizes the role of the mucosal barrier and how intestinal epithelial cells maintain barrier integrity through mucus secretion, formation of intercellular junctions, and production of antimicrobial peptides. The article also discusses how imbalances in regulation can lead to barrier damage and dysbiosis, contributing to inflammatory and metabolic diseases.
Article
Agriculture, Multidisciplinary
Jinfan Liu, Lu Fu, Fei Yin, Li Yin, Xiaomei Song, Hong Guo, Jianhui Liu
Summary: Crohn's disease (CD) is a chronic inflammatory bowel disease caused by dysfunction in the barrier function formed by polarized monolayer of epithelial cells. Diosmetin has been found to increase cell viability, maintain barrier integrity, and decrease the protein content of ABCG2 in Caco-2 cells and CD mice. Mechanistically, diosmetin attenuates the phosphorylation of AMPK, PI3K/AKT, and CREB, while AMPK inhibitor prevents the effect of diosmetin on tight junction proteins. These findings suggest that diosmetin improves barrier dysfunction in CD through the AMPK/AKT/CREB-mediated expression of ABCG2.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Mireille Alhouayek, Linda Stafberg, Jessica Karlsson, Sofia Halin Bergstrom, Christopher J. Fowler
SCIENTIFIC REPORTS
(2020)
Editorial Material
Biochemistry & Molecular Biology
Mireille Alhouayek, Gerard Bannenberg, Giulio G. Muccioli, Xavier Norel, Chengcan Yao, Nils Helge Schebb
PROSTAGLANDINS & OTHER LIPID MEDIATORS
(2020)
Review
Biochemistry & Molecular Biology
Mireille Alhouayek, Hafsa Ameraoui, Giulio G. Muccioli
Summary: Inflammatory bowel diseases (IBDs) such as Crohn's disease and ulcerative colitis are chronic conditions that are difficult to treat. Research has shown that lipid mediators play a crucial role in inflammation and immunity, and can have a significant impact on the pathophysiology of IBDs.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biochemistry & Molecular Biology
Pauline Bottemanne, Adrien Paquot, Hafsa Ameraoui, Owein Guillemot-Legris, Mireille Alhouayek, Giulio G. Muccioli
Summary: Inflammation is a critical component of many lung diseases, and studies have shown that only one type of oxysterol levels change during lung inflammation, with different effects on its levels in different disease models. Experiments demonstrated that this oxysterol levels increased in the acute lung inflammation model, leading to a decrease in inflammatory cytokine secretion.
Article
Biochemistry & Molecular Biology
Baptiste Buisseret, Owein Guillemot-Legris, Youssef Ben Kouidar, Adrien Paquot, Giulio G. Muccioli, Mireille Alhouayek
Summary: Pain is a cardinal sign of inflammation, with prostaglandins being major bioactive lipids involved in this process. The enzyme COX-2 not only synthesizes prostaglandins, but also metabolizes endocannabinoids to produce derivatives that may play a role in nociception. Further research on these endocannabinoid metabolites may provide insights into inflammatory pain mechanisms.
Article
Gastroenterology & Hepatology
Lina M. Olsson, Gudrun E. Lindmark, Anne C. E. Israelsson, Dejan Korkocic, Sten G. Hammarstrom, Marie-Louise K. C. Hammarstrom
Summary: This study compared the effectiveness of analyzing a selected group of biomarker mRNAs with routine methods for detecting lymph node metastases in colon cancer patients. The results showed that analyzing CEACAM5 and KLK6 mRNA levels improved the detection of tumor cells in lymph nodes, and increasing the tissue volume analyzed for CEACAM5 led to an increase in the number of positive nodes detected.
DISEASES OF THE COLON & RECTUM
(2021)
Article
Clinical Neurology
Pauline Bottemanne, Owein Guillemot-Legris, Adrien Paquot, Julien Masquelier, Michael Malamas, Alexandros Makriyannis, Mireille Alhouayek, Giulio G. Muccioli
Summary: The study reveals that NAAA inhibition significantly reduces pathological changes in a multiple sclerosis model, while FAAH inhibition is less effective, and simultaneous inhibition of NAAA and FAAH does not provide additional benefits.
Article
Biochemistry & Molecular Biology
Manar AbdelMageed, Hager Tarek H. Ismail, Lina Olsson, Gudrun Lindmark, Marie-Louise Hammarstrom, Sten Hammarstrom, Basel Sitohy
Summary: The study demonstrates the utility of measuring mRNA expression levels of CSC markers in predicting survival outcome and recurrence risk in colon cancer patients. LGR5 is identified as a particularly useful prognostic biomarker, and combining it with other biomarkers can enhance the accuracy of prediction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hafiz Abdul Khaliq, Sergio Ortiz, Mireille Alhouayek, Giulio G. Muccioli, Joelle Quetin-Leclercq
Summary: The anti-inflammatory potential of Convolvulus arvensis was evaluated and the results showed that the extracts and major constituents of the plant can significantly inhibit the expression of pro-inflammatory mediators, supporting its use in the treatment of inflammatory diseases.
Article
Biochemistry & Molecular Biology
Hager Tarek H. Ismail, Manar AbdelMageed, Gudrun Lindmark, Marie-Louise Hammarstrom, Sten Hammarstrom, Basel Sitohy
Summary: This study investigated the prognostic value of GPR55 in colon cancer. Combining GPR55 with CEA, CXCL16, or CXCL17 improved the discrimination between high and low expression groups. The GPR55/CEA ratio showed the best result, with a significant increase in mean survival time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Peter Flood, Aine Fanning, Jerzy A. Woznicki, Tadhg Crowley, Andrea Christopher, Alessandra Vaccaro, Aileen Houston, Sheila McSweeney, Sarah Ross, Aileen Hogan, Elizabeth Brint, Agnieszka Skowyra, Milan Bustamante, Monica Ambrose, Gerard Moloney, John MacSharry, Marie-Louise Hammarstrom, Margot Hurley, Christine Fitzgibbons, Eamonn M. M. Quigley, Fergus Shanahan, Syed A. Zulquernain, Jane McCarthy, G. Steven Dodson, Karim Dabbagh, Bradford L. McRae, Silvia Melgar, Ken Nally
Summary: The DNA sensor pathways play a crucial role in the pathogenesis of ulcerative colitis (UC), and are associated with the overactivation of inflammasome and type I interferon (IFN) signaling pathways. Furthermore, the study discovered that the combination of type I IFN (IFN-beta) and TNF-alpha can induce JAK-dependent inflammatory cell death in colonic organoids, independent of the inflammasome.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2022)
Review
Food Science & Technology
Hafiz Abdul Khaliq, Mireille Alhouayek, Joelle Quetin-Leclercq, Giulio G. G. Muccioli
Summary: Inflammation is a defensive response of the organism to various injuries. Chronic inflammatory diseases are challenging to treat, and phytochemicals may provide an interesting alternative approach. AMP-activated protein kinase plays an important role in regulating inflammatory responses. This review summarizes studies on phytochemicals that activate AMPK to improve chronic inflammatory disease models, and highlights factors to consider when investigating AMPK in the context of phytochemicals.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Nanoscience & Nanotechnology
Ariane Mwema, Pauline Bottemanne, Adrien Paquot, Bernard Ucakar, Kevin Vanvarenberg, Mireille Alhouayek, Giulio G. Muccioli, Anne des Rieux
Summary: Prostaglandin D2-glycerol ester (PGD2-G) was used as a targeting agent for neuroinflammation, and lipid nanocapsules (LNC) were used as a safe vehicle to transport PGD2-G to the central nervous system (CNS). PGD2-G-loaded LNC (PGD2-G-LNC) effectively reduced pro-inflammatory cytokine expression in activated microglial cells and the olfactory bulb. Coating LNC's surface with the cell-penetrating peptide, transactivator of transcription (TAT), increased its accumulation in the brain and reduced the expression of pro-inflammatory chemokines in the CNS.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Oncology
Gudrun Lindmark, Lina Olsson, Basel Sitohy, Anne Israelsson, Joel Blomqvist, Sara Kero, Tamer Roshdy, Mattias Soderholm, Annamaria Turi, Jessica Isaksson, Thorbjorn Sakari, Michiel Dooper, George Dafnis, Pehr Forsberg, Susanne Skovsted, Maria Wallden, Chih-Han Kung, Martin Rutegard, Johanna Nordmyr, Mans Muhrbeck, Sten Hammarstrom, Marie-Louise Hammarstrom
Summary: One fourth of colorectal cancer patients undergoing curative surgery will relapse, and lymph node metastasis is the most important prognostic factor. However, the current histopathological examination method is subjective and unable to provide information on tumor aggressiveness. The researchers developed a qRT-PCR test called ColoNode, which detects mRNA levels of specific biomarkers to estimate tumor cell load and aggressiveness. In a study of 196 colon cancer patients, ColoNode identified all patients detected by histopathology and an additional 9 patients. ColoNode was found to be a highly significant risk factor and surpassed histopathology in identifying patients at risk of relapse.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Emilie Moens de Hase, Camille Petitfils, Mireille Alhouayek, Clara Depommier, Pauline Le Faouder, Nathalie M. Delzenne, Matthias Van Hul, Giulio G. Muccioli, Nicolas Cenac, Patrice D. Cani
Summary: The newly identified bacterium D. welbionis J115(T) improves host metabolism in high-fat diet-fed mice through the production of bioactive lipids. These metabolic effects are associated with a whitening of brown adipose tissue and upregulation of genes related to PPAR-γ agonists.
JOURNAL OF LIPID RESEARCH
(2023)