Article
Environmental Sciences
Churaibhon Wisessaowapak, Apichaya Niyomchan, Daranee Visitnonthachai, Naphada Leelaprachakul, Piyajit Watcharasit, Jutamaad Satayavivad
Summary: Arsenic can lead to insulin and IGF-1 resistance in human neuroblastoma cells, disrupting cellular signaling and potentially affecting growth and neurite formation by inhibiting tyrosine kinase activity.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Medicine, Research & Experimental
Bin Zeng, Xiaoting Liao, Lei Liu, Caixia Zhang, Huaiyu Ruan, Bo Yang
Summary: The study found that T3 pretreatment significantly reduces myocardial infarct area, inhibits cardiomyocyte apoptosis and fibrosis, and improves left ventricular function post-MI. Furthermore, T3 can also promote angiogenesis, reduce inflammatory cytokines, and protect the heart from damage after MI.
Letter
Oncology
Wenjing Lei, Zhuoliang Liu, Zhiyuan Su, Panpan Meng, Chun Zhou, Xiaomei Chen, Zheng Hu, An Xiao, Miaomiao Zhou, Liping Huang, Yiyue Zhang, Xianhui Qin, Junping Wang, Fengxin Zhu, Jing Nie
Summary: Hyperhomocysteinemia (HHcy) is associated with thrombotic diseases and can increase platelet count. This study demonstrates that HHcy promotes megakaryocytes differentiation through the GH-PI3K-Akt axis and is independent of thrombopoietin (TPO). Melatonin administration effectively reverses Hcy-induced thrombopoiesis. This research provides new insight into the mechanisms by which HHcy confers an increased thrombotic risk.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2023)
Article
Cell & Tissue Engineering
Wen-Cheng Lo, Chi-Sheng Chiou, Feng-Chou Tsai, Chun-Hao Chan, Samantha Mao, Yue-Hua Deng, Chia-Yu Wu, Bou-Yue Peng, Win-Ping Deng
Summary: Nicotine may induce intervertebral disc degeneration, and platelet-derived biomaterials (PDB) can inhibit this process by modulating the IGF-1/AKT/IRS-1 signaling axis.
CELL TRANSPLANTATION
(2021)
Article
Hematology
Shilei Chen, Kangfu Sun, Baichuan Xu, Songlin Han, Song Wang, Yang Xu, Fang Chen, Mo Chen, Mingqiang Shen, Yukai Lu, Changhong Du, Mengjia Hu, Fengchao Wang, Junping Wang
Summary: Platelet shedding from mature megakaryocytes is crucial for increasing circulating platelets rapidly, and this study investigates the mechanisms behind it and identifies new therapeutic targets for thrombocytopenia.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Cell Biology
Jin-Feng Zhu, Yi Liu, Wen-Ting Li, Ming-Hui Li, Chao-Hui Zhen, Pei-Wei Sun, Ji-Xin Chen, Wen-Hao Wu, Wei Zeng
Summary: This study aimed to investigate the effect of Ibrutinib and ferroptosis inducers on colorectal cancer treatment and its underlying mechanism. The upregulation of Nrf2 was found to be correlated with CRC progression and antioxidant proteins. Ibrutinib increased the sensitivity of CRC cells to ferroptosis inducers by inhibiting Nrf2.
CELL DEATH & DISEASE
(2023)
Article
Agriculture, Dairy & Animal Science
Pradeep Krishna Javvaji, Arindam Dhali, Joseph Rabinson Francis, Atul P. Kolte, Sudhir C. Roy, Sellappan Selvaraju, Anjumoni Mech, Veerasamy Sejian
Summary: The study showed that IGF-1 treatment significantly improved the developmental competence of ovine oocytes, enhancing the maturation rate and embryo development. This effect was achieved through the regulation of the PI3K/Akt and apoptosis signaling pathways.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qin Zhao, Hong Su, Wei Jiang, Haodong Luo, Lu Pan, Yuan Liu, Ce Yang, Ying Yin, Lehua Yu, Botao Tan
Summary: This study investigated the effect of IGF-1 combined with OPN on neuronal axon growth and protein expression levels, and its underlying mechanism. The results showed that IGF-1 combined with OPN promoted axon growth through the IGF-1R/Akt/mTOR signaling pathway in lipid rafts, and the effect was superior to either agent alone. However, this effect was suppressed by the mTOR inhibitor rapamycin or the lipid raft cholesterol extraction agent M-beta-CD. Additionally, M-beta-CD significantly decreased the expression of p-IR. Western blot analysis revealed that the IGF-1 combined with OPN group had the highest expression levels of IR and p-IR in the neuronal lipid rafts. The enrichment of IR by IGF-1 combined with OPN was weakened, and p-IR was reduced when M-beta-CD was administered to the lipid rafts of neurons. This study demonstrated that IGF-1 combined with OPN promoted axon growth by activating the IGF-1R/Akt/mTOR signaling pathway in lipid rafts.
NEUROCHEMICAL RESEARCH
(2023)
Article
Cell Biology
Minle Li, Xue Zhao, Hongmei Yong, Jian Xu, Pengfei Qu, Shuxi Qiao, Pingfu Hou, Zhongwei Li, Sufang Chu, Junnian Zheng, Jin Bai
Summary: Transketolase (TKT), an important enzyme in the pentose phosphate pathway, plays a crucial role in maintaining ribose 5-phosphate levels and cell growth. Recent studies have shown that TKT is not only involved in liver cancer development but also in colorectal cancer progression. Abnormal upregulation of TKT expression is associated with poor prognosis in colorectal cancer, and TKT promotes colorectal cancer cell growth and metastasis by enhancing glycolysis through AKT phosphorylation.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Mei-Ling Li, Aparna Ragupathi, Nikhil Patel, Tatiana Hernandez, Jedrick Magsino, Guy Werlen, Gary Brewer, Estela Jacinto
Summary: mTORC2 regulates Akt phosphorylation by modulating the RNA-binding protein AUF1, which affects cellular metabolism and tumor progression.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Mei-Ling Li, Aparna Ragupathi, Nikhil Patel, Tatiana Hernandez, Jedrick Magsino, Guy Werlen, Gary Brewer, Estela Jacinto
Summary: The RNA-binding protein AUF1 plays a crucial role in modulating mTORC2/Akt signaling by promoting membrane localization of Akt and facilitating its phosphorylation. AUF1 also mediates the phosphorylation of the mTORC2-regulated metabolic enzyme glutamine fructose-6-phosphate amidotransferase 1. These findings have significant implications for the therapeutic targeting of cancers with upregulated mTORC2/Akt signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Tri Wahyuni, Arisa Kobayashi, Shota Tanaka, Yoshiaki Miyake, Ayaha Yamamoto, Anton Bahtiar, Shota Mori, Yusuke Kametani, Masashi Tomimatsu, Kotaro Matsumoto, Makiko Maeda, Masanori Obana, Yasushi Fujio
Summary: MaR1 induces cardiomyocyte hypertrophy through the RORa/IGF-1/PI3K/Akt pathway, which may play a key role in orchestrating the resolution of inflammation with myocardial repair.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Neeraj Singh, Marc R. Benoit, John Zhou, Brati Das, Jose Davila-Velderrain, Ma Nolis Kellis, Li-Huei Tsai, Xiangyou Hu, Riqiang Yan
Summary: BACE-1 plays a role in regulating the transition of microglia in Alzheimer's disease. Deficiency of BACE-1 leads to increased expression of certain transcription factors involved in the transition from homeostatic to highly phagocytic microglia. Similar transition-state microglia in AD brains are associated with decreased levels of BACE-1 expression.
Article
Biochemistry & Molecular Biology
Ting Liu, Yang Li, Baoyu Yang, Haozhen Wang, Chen Lu, Alan K. Chang, Xiuting Huang, Xiujin Zhang, Ziyin Lu, Xiuli Lu, Bing Gao
Summary: The study revealed that reducing cholesterol levels can lead to neural apoptosis by impairing the IGF-1-Akt survival signaling pathway in the brain, potentially contributing to the pathogenesis of Alzheimer's disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Han Dai, Wenjing Hu, Lianying Zhang, Feiyu Jiang, Xiongmin Mao, Gangyi Yang, Ling Li
Summary: Research showed that FGF21 was significantly downregulated in prostate cancer tissues and cell lines, and inhibited PCa cell proliferation and promoted apoptosis through facilitated autophagy.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Na Zhao, Guojian Wang, Shuang Long, Dengqun Liu, Jining Gao, Yang Xu, Cheng Wang, Aiping Wang, Fengchao Wang, Yuhui Hao, Xinze Ran, Junping Wang, Yongping Su, Tao Wang
Summary: The study identifies an endogenous pattern involving Spink7 in the immune compartment as a key player in controlling chemokines/cytokines production, contributing to the resolution of inflammation in colitis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Chemistry, Multidisciplinary
Cheng Wang, Shaobo Wang, Yin Chen, Jianqi Zhao, Songling Han, Gaomei Zhao, Jing Kang, Yong Liu, Liting Wang, Xiaoyang Wang, Yang Xu, Song Wang, Yi Huang, Junping Wang, Jinghong Zhao
Summary: Membrane nanoparticles derived from ACE2-rich cells were found to effectively block SARS-CoV-2 infection by trapping viral ligands and reducing viral recruitment to host cells. These ACE2-NPs also inhibited viral entry into cells and blocked infection, providing a promising avenue for COVID-19 treatment.
Article
Chemistry, Medicinal
Cheng Wang, Shaobo Wang, Daixi Li, Peiqin Chen, Songling Han, Gaomei Zhao, Yin Chen, Jianqi Zhao, Jiachuan Xiong, Jingfei Qiu, Dong-Qing Wei, Jinghong Zhao, Junping Wang
Summary: The study showed that human cathelicidin LL37 can block the viral S1 and cloak ACE2 simultaneously, providing a potential candidate for COVID-19 prevention and treatment.
ACS INFECTIOUS DISEASES
(2021)
Article
Mathematical & Computational Biology
Daixi Li, Cheng Wang, Shaobo Wang, Aamir Mehmood, Jiang Gu, Xin Cheng, Peiqin Chen, JingFei Qiu, Jinghong Zhao, Junping Wang, Dongqing Wei
Summary: This study proposed a potent peptide to prevent SARS-CoV-2 infection by inhibiting the binding of RBD to ACE2, which was found to suppress SARS-CoV-2 S pseudovirion infection with a half-maximal inhibitory concentration (IC50) of 18.52 μg/mL.
INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Songling Han, Gaomei Zhao, Zhuanzhuan Wei, Yin Chen, Jianqi Zhao, Yongwu He, Ying-Juan He, Jining Gao, Shilei Chen, Changhong Du, Tao Wang, Wei Sun, Yi Huang, Cheng Wang, Junping Wang
Summary: A short peptide inhibitor GK-7 was designed to reduce the binding of SARS-CoV-2 S1 to host cells and inhibit viral infection; Pulmonary delivery of GK-7 via intranasal administration showed no toxicity in mice; This study provides a promising candidate for COVID-19 treatment.
Article
Biophysics
Yuliang Li, Chengli Zhang, Yingjuan He, Jining Gao, Wei Li, Lin Cheng, Fengjun Sun, Peiyuan Xia, Qian Wang
Summary: MicroRNAs (miRNAs) have been extensively studied as circulating biomarkers for early diagnosis and prognosis of many human diseases. This study proposes a generic electrochemical strategy integrated with molecular beacon-like catalyzed hairpin assembly (mCHA) circuit and MOF@Au@G-triplex/hemin nanozyme for ultrasensitive detection of miR-721. With the assistance of the mCHA circuit, double-amplified nanozymes and sensing interface, this biosensor demonstrates a low detection limit and high specificity, providing a promising potential method for the early diagnosis of acute myocarditis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Virology
Zhuanzhuan Wei, Yiyi Jiang, Gaomei Zhao, Chenwenya Li, Songling Han, Yin Chen, Tao Wang, Tianmin Cheng, Junping Wang, Cheng Wang
Summary: This study investigated the influence of irradiation on the vulnerability of cancer cells to SARS-CoV-2 and revealed that irradiation promotes viral infection through enhanced sphingolipid metabolism. The study also uncovered the underlying mechanism of sphingolipid metabolism in irradiation-induced SARS-CoV-2 infection.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Xing Luo, Yanan Tong, Xiaoqiang Zeng, Yan Ye, Yun Yang, Zhen Song, Zelong Zhang, Haibo Li, Jining Gao, Xuhu Mao, Hao Zeng, Quanming Zou, Hongwu Sun
Summary: This article examines a series of in vitro protocols for evaluating the cellular response efficacy of a novel vaccine adjuvant, nanoemulsion ophiopogonin D (NOD). The protocols include cell cytotoxicity testing, detection of cytokine levels and immune cell numbers, as well as observation of antigen uptake ability in dendritic cells. The study confirms the activation of macrophages in response to the adjuvant. This research provides other researchers with direct and effective experimental approaches to evaluate novel vaccine adjuvants.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Gaomei Zhao, Changsheng Jia, Cheng Zhu, Minchao Fang, Chenwenya Li, Yin Chen, Yingjuan He, Songling Han, Yongwu He, Jining Gao, Tao Wang, Cheng Wang, Junping Wang
Summary: With the increase in infections caused by resistant bacteria globally, there is a need for alternative methods to antibiotics. This study truncated the structure of human defensin (HD) 5 by extracting the highly conserved gamma-core motif, leading to improved antibacterial activity. The derivative RC18, created by replacing Glu and Ser residues with Arg, exhibited stronger potency against methicillin-resistant S. aureus (MRSA) and showed promising results in in vivo experiments.
Article
Chemistry, Multidisciplinary
Yongwu He, Mo Chen, Jing Wang, Gaomei Zhao, Songling Han, Yang Xu, Yin Chen, Cheng Wang, Junping Wang
Summary: This article describes a comprehensive experiment designed for undergraduate chemistry courses, aiming to enhance students' understanding of the photocatalytic effect of ZnO nanomaterials. The experiment involves the degradation of organic dyes using a ZnO-coated graphene oxide nanocomposite and introduces various characterization techniques. By observing the degradation behavior and reusability of the nanocomposite, as well as investigating its mechanism using different scavengers, students can gain insights into the catalytic properties of ZnO/GO. This experiment is recommended for its potential to promote engagement in the field of applied chemistry among undergraduates.
JOURNAL OF CHEMICAL EDUCATION
(2023)
Article
Medicine, Research & Experimental
Shaobo Wang, Yin Chen, Songling Hang, Yong Liu, Jining Gao, Yinghui Huang, Wei Sun, Junping Wang, Cheng Wang, Jinghong Zhao
Summary: This study demonstrates an effective therapeutic option by using Se@BSA nanoparticles to treat IRI-induced AKI. By correcting selenium deficiency and inhibiting inflammatory responses, Se@BSA nanoparticles show promising results in mitigating AKI and preventing fibrosis formation.
Article
Engineering, Environmental
Yongwu He, Songling Han, Gaomei Zhao, Jiaohua Luo, Changsheng Jia, Yin Chen, Qi Liu, Jining Gao, Cheng Wang, Junping Wang
Summary: In this study, a nanohybrid composed of zinc oxide and carboxyl-riched graphene oxide was prepared, which could efficiently degrade microcystin-leucine arginine and three quinolone antibiotics in water. The material was non-toxic to cells and could alleviate the damage caused by the toxins to animals.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
