Article
Biotechnology & Applied Microbiology
Feiyan Xue, Zihui Liu, Yue Yu, Yangjie Wu, Yuxin Jin, Mingfeng Yang, Lanqing Ma
Summary: Researchers cloned the cDNA of Rhodotorula glutinis PAL (RgPAL) and introduced codon optimization to enhance its protein expression and enzyme activity in Escherichia coli. This improvement allowed for the successful expression and purification of the recombinant enzyme PAL-opt, which exhibited high specific activity for substrate L-phenylalanine and L-tyrosine. This bifunctional enzyme provides a promising foundation for future applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu Qin, Qiue Li, Qiuju An, Dexin Li, Sipei Huang, Yongyang Zhao, Weijia Chen, Jiayu Zhou, Hai Liao
Summary: Drought is a key threat to plants and agriculture, and the role of the enzyme phenylalanine ammonia lyase (PAL) in drought tolerance has been explored in this study. A PAL member called FuPAL1 was isolated from the alpine pharmaceutical plant Fritillaria unibracteata. FuPAL1 was found to play a role in the ammonization process and was involved in substrate binding and catalysis. Transgenic Arabidopsis plants with FuPAL1 showed increased levels of lignin and salicylic acid and enhanced drought tolerance. These findings suggest that FuPAL1 could be a valuable target for improving drought resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Hassan S. Al-Zahrani, Hesham E. Alharby, Khalid Rehman Hakeem, Reiaz Ul Rehman
Summary: The study found that the application of zinc can alleviate the negative effects of salt stress on plant growth to a certain extent by improving physiological and biochemical changes, including increased shoot and root length, enhanced chlorophyll and carotenoid contents, and increased enzymatic antioxidant activities.
Article
Chemistry, Physical
Hanan L. L. Messiha, Nigel S. S. Scrutton, David Leys
Summary: Biosynthetic routes for cost-efficient, eco-friendly, and sustainable production of compounds like styrene are urgently needed. The traditional biosynthesis of styrene from L-phenylalanine via trans-cinnamate is limited by styrene toxicity. This study demonstrates that using an E. coli consortium, whole-cell cascade biotransformation can efficiently convert L-phenylalanine to styrene, avoiding the issue of styrene toxicity and the need for enzyme purification.
Article
Chemistry, Physical
Chun-Yen Hsieh, Yi-Hao Huang, Hui-Hsuan Yeh, Pei-Yu Hong, Che-Jen Hsiao, Lu-Sheng Hsieh
Summary: Bambusa oldhamii BoPAL4 exhibits broad substrate specificity, with optimal reaction pH and temperature varying for different substrates. A major substrate specificity site mutant, BoPAL4-H123F, shows improved affinity for L-phenylalanine. BoPAL4 is confirmed to utilize L-phenylalanine, L-tyrosine, and L-DOPA as substrates.
Article
Multidisciplinary Sciences
Kristin J. Adolfsen, Isolde Callihan, Catherine E. Monahan, Per Greisen, James Spoonamore, Munira Momin, Lauren E. Fitch, Mary Joan Castillo, Lindong Weng, Lauren Renaud, Carl J. Weile, Jay H. Konieczka, Teodelinda Mirabella, Andres Abin-Fuentes, Adam G. Lawrence, Vincent M. Isabella
Summary: In this study, a more potent EcN-based PKU strain was developed through the optimization of whole cell PAL activity, showcasing approximately two-fold increase in in vivo PAL activity compared to the previous strain. The biosensor-based ultra-high-throughput screening approach utilized in this study could potentially lead to improved in vivo performance for PKU treatment in the future.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Guohui Li, Cheng Song, Muhammad Aamir Manzoor, Daoyuan Li, Yunpeng Cao, Yongping Cai
Summary: This study identified three PAL genes in the pear genome and revealed their significant impact on stone cell and lignin contents in pear fruits. Additionally, a transcription factor PbWLIM1 that regulates the transcription level of PbPAL2 was also discovered.
Article
Plant Sciences
Kazem Ghassemi-Golezani, Salar Farhangi-Abriz
Summary: Biochar reduces lignification and enhances nutrient uptake by plant roots. Addition of biochar promotes root growth, activity, and the absorption of essential elements, while reducing oxidative damages caused by toxic substances.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Engineering, Environmental
Jialian Wei, Sisi Liao, Muzi Li, Bin Zhu, Hongcheng Wang, Lei Gu, Huayan Yin, Xuye Du
Summary: Cadmium is a toxic heavy metal that poses a serious threat to crop safety and human health. This study investigated the molecular mechanisms underlying the response of Aegilops tauschii, a common wheat donor, to cadmium stress. Key genes involved in cadmium stress response were identified, including AetSRG1, which played a crucial role in reducing cadmium accumulation and increasing antioxidant production. These findings provide new insights into the development of low cadmium wheat.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Plant Sciences
AiLian Liu, Yue Zhu, YuHao Wang, TianYu Wang, ShuPing Zhao, Kai Feng, LiangJun Li, Peng Wu
Summary: In this study, PAL genes in E. ferox were identified and functionally analyzed. EfPAL1 and EfPAL2 were found to be key genes in flavonoid biosynthesis, and a regulatory network for EfPAL2 was established. These findings provide novel information for understanding the mechanism of flavonoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Fangyu Mo, Long Li, Chao Zhang, Chenghui Yang, Gong Chen, Yang Niu, Jiaxin Si, Tong Liu, Xinxin Sun, Shenglan Wang, Dongdong Wang, Qin Chen, Yue Chen
Summary: This study identified 14 StPAL genes in potato for the first time and investigated their expression patterns under different stresses and hormone treatments. The results suggest that these genes may be involved in stress defense and chemical defense mechanisms in potato.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Fariborz Habibi, Ali Sarkhosh, Fabian Guillen, Maria Serrano, Daniel Valero
Summary: The effects of glycine betaine (GB) and methyl salicylate (MeSA) treatments, alone and in combination, on the chilling tolerance and bioactive compounds of blood orange cv. Moro during cold quarantine storage were investigated. The combined MeSA and GB treatment showed the highest effectiveness in enhancing the fruit's chilling tolerance and preserving bioactive compounds.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yannik Brack, Chenghai Sun, Dong Yi, Uwe T. Bornscheuer
Summary: p-Coumaric acid (p-CA) is a key precursor for flavonoid biosynthesis, and tyrosine ammonia lyases (TALs) catalyze the synthesis of p-CA from l-tyrosine. In this study, 875 putative TALs and 46 putative phenylalanine/tyrosine ammonia lyases (PTALs) were analyzed, and 5 TALs and 3 PTALs were characterized and found to exhibit the proposed enzymatic activity. The TAL from Chryseobacterium luteum sp. nov (TAL(clu)) showed the highest affinity and conversion efficiency towards l-tyrosine. Optimization of reaction conditions resulted in p-CA yields of 2.03 g/L after 8 hours by TAL(clu) and 2.35 g/L after 24 hours by TAL from Rivularia sp. PCC 7116 (TAL(rpc)). These TALs are potential candidates for the production of flavonoid precursor p-CA using whole-cell systems.
Article
Biochemistry & Molecular Biology
Lu Yao, Huanyu Zhang, Yirong Liu, Qiushuang Ji, Jing Xie, Ru Zhang, Luqi Huang, Kunrong Mei, Juan Wang, Wenyuan Gao
Summary: In this study, we developed a multi-modular strategy to enhance the yield of ginsenoside Rg(3) in Panax ginseng. By improving the enzymatic efficiency and metabolic flux, we successfully increased the accumulation of ginsenoside Rg(3). The established high-production system could potentially be used for commercial production of ginsenoside Rg(3) for pharmaceutical purposes.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Agronomy
Yang Yu, Yingjie Wang, Yue Yu, Peiyong Ma, Zhaodong Jia, Xiaoding Guo, Yizhi Xie, Xiaofeng Bian
Summary: The study identified IbPAL1 gene in sweetpotato which is associated with CGA accumulation. Overexpression of IbPAL1 promoted CGA accumulation and affected different tissues, providing a theoretical foundation for sweetpotato CGA biosynthesis.
Article
Food Science & Technology
Xiaoqi Zhao, Guoqiang Chen, Fan Wang, Han Zhao, Yuxi Wei, Lijuan Liu, Haibo Zhang
Summary: In this study, a novel exopolysaccharide (EPS) secreted by the extreme salt-tolerant Bacillus subtilis LR-1 was researched. The EPS showed strong antioxidant activity and thickening performance, suggesting its potential application in functional food and related industries.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Wenzhi Bao, Xing Li, Jinfeng Liu, Rong Zheng, Lijuan Liu, Haibo Zhang
Summary: KDC4427 is a highly efficient PPDC enzyme that plays a crucial role in the biosynthesis of 2-phenylethanol (2-PE) and exhibits high decarboxylation activity for IPyA. Its 3D structure is 96% similar to EcIPDC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Guoqiang Chen, Zhenxiang Xu, Fan Wang, Lijuan Liu, Yuxi Wei, Jinglong Li, Liang Zhang, Kai Zheng, Lijun Wu, Xiao Men, Haibo Zhang
Summary: The extraction and composition of a new type of microbial polysaccharide FM-EPS were explored in this study, and its antioxidant and antiproliferative activities were investigated. The results indicate that FM-EPS has potential applications.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xuemin Ding, Weixia Ye, Bin Tan, Qiaoyun Song, Yuchan Chen, Wei Liu, Lili Sun, Wei Tang, Yulei Qiao, Qingbo Zhang, Haibo Zhang, Ying Wang, Weimin Zhang, Changsheng Zhang, Wenjun Zhang
Summary: Three new cytochalasins, talachalasins A-C (1-3), were discovered from a deep-sea-derived fungus called Talaromyces muroii sp. SCSIO 40439. The structures and absolute configurations of 1-3 were determined using various analytical techniques. Talachalasins A-C (1-3) are unique cytochalasans with a 168-methyl group, which distinguishes them from over 500 known CYTs. Talachalasin C (3) is a novel 20,21-seco-cytochalasans with a 2-oxabicyclo-[3.3.1]nonan-3-one unit. Talachalasin A (1) exhibited moderate cytotoxic activity against four tumor cell lines (IC50: 3.40-10.02 μM). Talachalasin B (2) demonstrated comparable anti-RSV and HSV-1 activities (IC50: 12.5 p.M or IC50: 20.0 μM).
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Guoqiang Chen, Xin Liang, Xiao Men, Lijuan Liu, Fan Wang, Xichang Bao, Haibo Zhang
Summary: Researchers have developed a novel high-conductivity thin film by spin-coating a layer of polyvinyl alcohol (PVA) on the surface of bacterial cellulose (BC) infused with silver nanowires (AgNWs). This thin film addresses the issues of high AgNWs-AgNWs contact resistance and poor long-term stability, making it suitable for manufacturing a new generation of electronic skin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yajing Yang, Guoqiang Chen, Xiaoqi Zhao, Xiaohe Cao, Lei Wang, Jingjiu Mu, Fenghui Qi, Lijuan Liu, Haibo Zhang
Summary: Exopolysaccharides produced by edible microorganisms, such as S1 and S2, have excellent physicochemical properties and significant biological activity, making them advantageous for the food and pharmaceutical industries. Both S1 and S2 exhibited good thermal stability and potent hydroxyl radical scavenging activity, with S1 also possessing strong iron-reducing capacity. In vitro antitumor assays showed that S1 and S2 significantly inhibited the proliferation of Hela, HepG2, and PC-9 cancer cells, with PC-9 being more sensitive to S1. These findings suggest that S1 and S2 have great potential as natural antioxidants and candidates for cancer treatment in the food and pharmaceutical industries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Bin Tan, Qingbo Zhang, Jieyi Li, Yiguang Zhu, Liping Zhang, Wenjun Zhang, Haibo Zhang, Yang Chen, Changsheng Zhang
Summary: The installation of halogen atoms into aromatic and less activated polyketide substrates by halogenases is a powerful strategy to tune the bioactivity, bioavailability, and reactivity of compounds. In this study, the halogenase TotH was confirmed to catalyze the C-4 chlorination in the biosynthetic pathway of totopotensamide A (1), leading to the formation of ClMeDPG. Six new deschloro totopotensamide (TPM) congeners TPMs H2-H7 (5-10) were isolated and their structures were elucidated. Additionally, the cytotoxicity of compounds 5, 6, 8, and 9 against several cell lines was evaluated.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Environmental Sciences
Xiaoling Liu, Guangmei Wang, Haibo Zhang, Guangxuan Han, Kexin Li, Andong Wang
Summary: The availability and stoichiometry ratio of nitrogen (N) and phosphorus (P) are important factors in plant trophic dynamics and primary production. However, the response of supratidal wetland herbs to varying N and P supplies is not well understood. In this 4-year field manipulation experiment, the aboveground biomass, leaf N and P concentrations, and N:P ratios of two dominant herbs were examined under different nutrient supply levels and N:P supply ratios. The results showed that the leaf trophic dynamics and aboveground biomass of the herbs were influenced by both overall supply level and N:P supply ratio.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Plant Sciences
Lijuan Wang, Mengyi Zhu, Qingbo Zhang, Shilan Zhai, Yiguang Zhu, Haibo Zhang, Changsheng Zhang
Summary: In this study, an alternative biological route to produce structurally diverse fidaxomicin aglycones was developed by refactoring the promoter and expressing it in a different host. The resulting titers of the five fidaxomicin aglycones ranged from 4.9 to 15.0 mg L-1. Overall, these findings demonstrate the potential of using a biological strategy to construct and diversify fidaxomicin aglycones.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemical Research Methods
Mengyi Zhu, Lijuan Wang, Haibo Zhang, Liping Zhang, Bin Tan, Qi Huang, Yiguang Zhu, Changsheng Zhang
Summary: Ribosome-targeting oligosaccharides, everninomicins (EVNs), are promising drug leads with a unique mode of action. By duplicating the biosynthetic gene cluster (BGC) in Micromonospora sp. SCSIO 07395, the production of EVNs was enhanced, allowing for bioactivity evaluation. EVNs showed significant inhibition against multidrug-resistant bacteria, with potency comparable or superior to vancomycin, linezolid, and daptomycin. The BGC duplication strategy successfully increased the titer of bioactive EVN M from trace amounts to 98.6 mg L-1, demonstrating the utility of a bioengineering approach for enhanced production and chemical diversification of EVNs.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ge Zhang, Fan Wang, Shuaijun Deng, Guoqiang Chen, Haobao Liu, Haibo Zhang
Summary: This study aimed to obtain a strain capable of producing pectate lyase with good pH stability. Bacillus altitudinis CAS-WZS-08, producing pectate lyase with good pH stability, was isolated. The optimal fermentation conditions for pectate lyase production were determined, and the purified protein was identified as pectate lyase with excellent juice extraction ability.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Hongshuang Liu, Senbiao Fang, Lin Zhao, Xiao Men, Haibo Zhang
Summary: This study identified a novel key site, T182A, in the pentalenene synthase enzyme, which not only affected the solubility and activity of the enzyme, but also influenced the specificity of the product. The study demonstrated the crucial role of the 182 active site in stabilizing and guiding the regioselectivity of carbocations in the reaction.
BIOENGINEERING-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Meijie Li, Rumeng Yang, Jing Guo, Min Liu, Jianming Yang
Summary: This study successfully improved the thermostability of isoprene synthase through directed evolution. A tripartite protein folding system was used for high-throughput screening, resulting in the identification of three variants with higher stability. In vitro stability tests confirmed the increased stability of the variants. One of the variants also showed improved isoprene production.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Food Science & Technology
Feng Zeng, Meiying Lai, Quancen Li, Haibo Zhang, Zhixian Chen, Shiyu Gong, Xiaoyan Liu, Bin Liu
Summary: In this study, the antioxidant and anti-aging activities of mannoprotein-rich yeast cell wall enzymatic hydrolysate (MYH) were investigated using the Caenorhabditis elegans (C. elegans) model. The results showed that MYH could increase the lifespan and anti-stress ability of C. elegans by enhancing the activity of antioxidant enzymes and decreasing levels of oxidative stress and apoptosis. Additionally, MYH was found to modulate the expression of specific mRNAs related to antioxidant and anti-aging pathways. Furthermore, MYH was shown to improve the gut microbiota composition and metabolite levels in C. elegans. This study contributes to understanding the antioxidant and anti-aging activities of microorganisms and the development of functional foods.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Computer Science, Information Systems
Xiao Liu, Xingyao Zeng, Chengxiang Hao, Haibo Zhang, Zhongjun Yu, Ting Lv, Meng Li, Zhen Zhang
Summary: In this paper, a novel structure of Ka-band based phased array antenna with calibration function is proposed. The antenna adopts the active phased array system and works in the dual polarization separation mode. The schematic diagram and design details of the Ka-band antenna are provided. The transceiver network and calibration network are discussed, and simulation and measurement results are shown.
CMC-COMPUTERS MATERIALS & CONTINUA
(2023)