Article
Food Science & Technology
Monica Jimenez-Carretero, Javier Rodriguez-Lopez, Cristina Ropero-Moreno, Juan Granada, Josemaria Delgado-Martin, Manuel Martinez-Bueno, Antonia Fernandez-Vivas, Concepcion Jimenez-Lopez
Summary: The development of improved systems for rapid detection of trace infectious agents is essential. Magnetic nanoparticles (MNPs) are an interesting alternative due to their large surface area for maximizing interaction with microorganisms and their magnetic susceptibility for concentration using an external magnetic field. This study synthesized biomimetic magnetic nanoparticles (BMNPs) using MamC protein and demonstrated their efficacy in concentrating and detecting bacteria without the need for post-production coating. The system achieved specific detection of Staphylococcus aureus down to 10 CFU/mL using qPCR, making it a cost-effective alternative for bacteria detection in fluid samples.
Article
Chemistry, Analytical
Sizhe Xie, Hairui Wang, Nannan Li, Yulong Liu, Jianfeng Wu, Yongnan Xu, Jianwei Xie
Summary: A facile, rapid, and sensitive surface enhanced Raman spectroscopy (SERS) method for the analysis of cyanide in blood samples has been developed, which could be a powerful tool for the diagnosis of cyanide poisoning in clinical and forensic applications.
MICROCHEMICAL JOURNAL
(2022)
Article
Engineering, Biomedical
Bernhard Friedrich, Stefan Lyer, Christina Janko, Harald Unterweger, Regine Brox, Sarah Cunningham, Silvio Dutz, Nicola Taccardi, Floris J. Bikker, Katrin Hurle, Heidi Sebald, Malte Lenz, Erdmann Spiecker, Lars Fester, Holger Hackstein, Richard Strauss, Aldo R. Boccaccini, Christian Bogdan, Christoph Alexiou, Rainer Tietze
Summary: This study demonstrates an efficient strategy for the clearance of bacterial pathogens in bloodstream infections using magnetic particles conjugated with specific peptides, resulting in reduced cytokine release. The approach shows good compatibility with cells and blood, without interfering with host structures. It also has the potential to shorten diagnostic times for bloodstream infections and accelerate targeted antibiotic therapy.
ACTA BIOMATERIALIA
(2022)
Article
Materials Science, Multidisciplinary
Cheng Gao, Cheryl H. T. Kwong, Mian Tang, Jinwei Liu, Hiotong Kam, Shengke Li, Simon M. Y. Lee, Chunhai Fan, Hua-Zhong Yu, Ruibing Wang
Summary: This study develops intracellularly gelated macrophages (GMs) to neutralize bacteria and endotoxins and achieve efficient management of bacterial infection and targeted alleviation.
Article
Chemistry, Analytical
Francois Huber, Hans Peter Lang, Stefanie Heller, Julia Anna Bielicki, Christoph Gerber, Ernst Meyer, Adrian Egli
Summary: This study demonstrates the use of a rapid and sensitive nanomechanical sensor array for early detection of bacterial sepsis. By extracting total RNA from patient blood samples and utilizing 16S-rRNA hybridization and species-specific probes on the sensor array, the presence of clinically relevant bacterial species such as Escherichia coli and Staphylococcus aureus was successfully identified. This technology shows potential for revolutionizing sepsis diagnostics.
Article
Chemistry, Multidisciplinary
Sidi Liu, Huibo Wang, Le Yu, Yijin Ren, Hjalmar R. Bouma, Jian Liu, Henny C. van der Mei, Henk J. Busscher
Summary: In this study, the authors used a microfluidic device equipped with a bacterially activated, macrophage-membrane-coating on nanowired-Si adsorbent surfaces to achieve rapid bacterial detection and Gram-identification in bacterially contaminated blood. The results showed the potential of this platform for rapid diagnosis of bacterial sepsis, with accurate identification of bacterial strains and guidance for antibiotic selection.
Article
Biochemical Research Methods
Chang He, Zhenyi Ke, Kai Liu, Jiasheng Peng, Qinghui Yang, Lixiang Wang, Guangfu Feng, Jun Fang
Summary: In this study, a colorimetric and photothermal dual-signal point-of-care testing (POCT) sensing platform based on CeO2-TMB was developed for visualizing acetylcholinesterase (AChE) activity in liver-injured mice. This method compensates for false positives of single signal detection, enabling rapid, low-cost portable detection of AChE. Furthermore, the CeO2-TMB sensing platform allows for the diagnosis of liver injury and provides an effective tool for studying liver disease in basic medicine and clinical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Na Wang, Juan Yang, Zhi Luo, Dongmiao Qin, Yusheng Wu, Biyang Deng
Summary: A dual-emitting electrochemiluminescence (ECL) immunosensor was constructed using manganese dioxide nanoflowers (MnO(2)NFs) and zinc sulfide quantum dots (ZnSQDs), which sensitively detected procalcitonin (PCT) in human serum. The nanocomposite of MnO(2)NFs, ZnSQDs, and silver nanoparticles acted as cathodic ECL emitters to enhance the detection sensitivity of PCT by shortening the electron-transfer path and reducing energy loss.
Article
Biotechnology & Applied Microbiology
Yanqi Wu, Liping Bai, Chengfu Ye, Yuhong Guan, Kunming Yan, Hui Chen, Zhihong Jiang
Summary: A real-time fluorescent loop-mediated isothermal amplification (LAMP) detection system has been developed in this study, which is rapid and accurate for pathogen detection. The system shows consistent results with quantitative PCR (qPCR) and clinical results, with an accuracy of 100%.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jinyeop Lee, Abdurhaman Teyib Abafogi, Sujin Oh, Ho Eun Chang, Wu Tepeng, Daekyu Lee, Sungsu Park, Kyoung Un Park, Yun Ji Hong
Summary: Bacterial contamination is a significant problem in transfusion medicine, but real-time PCR using antibiotic-conjugated magnetic nanobeads offers a rapid and sensitive detection method with minimal blood components.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Analytical
Ying Lan, Baixun He, Cherie S. Tan, Dong Ming
Summary: Aptamers are functional recognition ligands with high specificity and affinity, which can be utilized in biosensors for sensing biomolecules. Aptasensors, based on aptamers, are specific, sensitive, and cost-effective, and have shown advantages when combined with smartphone devices for point-of-care testing.
Article
Chemistry, Analytical
Hyo Geun Lee, Wook Choi, Seung Yun Yang, Dong-Ho Kim, Sung-Gyu Park, Min-Young Lee, Ho Sang Jung
Summary: A paper-based surface-enhanced Raman scattering (SERS) substrate was developed for rapid detection of respiratory bacterial DNA, coupled with polymerase chain reaction (PCR). The PCR-coupled SERS method showed enhanced detection capability after 10 amplification cycles, enabling low-cycle amplification and sensitive DNA sensing.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Xuekun Bai, Zhengzheng Wang, Weiqiang Li, Fangbin Xiao, Jin Huang, Qian Xu, Hengyi Xu
Summary: Listeria monocytogenes is a serious food-borne pathogen that poses a threat to global public health. This study successfully developed an Amp-MBs-qPCR strategy for the rapid and specific detection of L. monocytogenes in milk samples. The strategy demonstrated high sensitivity and specificity, with a capture efficiency higher than 90% and a limit of detection of 101-102 CFU/mL.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Xinghui Yu, Yongqiang Ma, Siyuan Liu, Chunchun Qi, Weiqi Zhang, Wen Xiang, Zhaoxian Li, Kai Yang, Shaoxian Duan, Xinrao Du, Jian Yu, Yan Xie, Zicheng Wang, Wentao Jiang, Li Zhang, Xiaodong Lin
Summary: Post-operative pathogenic infections in liver transplantation pose a serious threat to human health. Researchers have developed a chemiluminescence-based method for the rapid and sensitive detection of Staphylococcus aureus. By integrating specific capture and separation techniques, this portable device, integrated with a smartphone, enables specific detection of S. aureus with high antibacterial efficiency. The method is simple to use and cost-effective, demonstrating great potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Applied
Nipaporn Sriplai, Supree Pinitsoontorn
Summary: Bacterial cellulose possesses unique and interesting properties, and can be endowed with magnetic characteristics by incorporating magnetic nanoparticles, offering promising potential in medical, environmental, and electronic applications.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Multidisciplinary
Angelo Frei, Maite Amado, Matthew A. Cooper, Mark A. T. Blaskovich
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Biology
Miranda E. Pitt, Son H. Nguyen, Tania P. S. Duarte, Haotian Teng, Mark A. T. Blaskovich, Matthew A. Cooper, Lachlan J. M. Coin
Article
Biochemistry & Molecular Biology
Amelia A. Fuller, Amy B. Dounay, Douglas Schirch, Daniel G. Rivera, Karl A. Hansford, Alysha G. Elliott, Johannes Zuegg, Matthew A. Cooper, Mark A. T. Blaskovich, Jacob R. Hitchens, Sarah Burris-Hiday, Kristiana Tenorio, Yanira Mendez, J. Geno Samaritoni, Martin J. O'Donnell, William L. Scott
ACS CHEMICAL BIOLOGY
(2020)
Article
Chemistry, Medicinal
Nusaibah Abdul Rahim, Yan Zhu, Soon-Ee Cheah, Matthew D. Johnson, Heidi H. Yu, Hanna E. Sidjabat, Mark S. Butler, Matthew A. Cooper, Jing Fu, David L. Paterson, Roger L. Nation, John D. Boyce, Darren J. Creek, Phillip J. Bergen, Tony Velkov, Jian Li
Summary: Carbapenem-resistant Klebsiella pneumoniae is classified as an Urgent Threat by the CDC, while the combination of polymyxin and chloramphenicol has a synergistic killing effect against NDM-producing K. pneumoniae. Metabolomic and GSMN modeling results highlight the potential significance of this synergistic mechanism in developing precision antimicrobial chemotherapy in patients.
ACS INFECTIOUS DISEASES
(2021)
Review
Pharmacology & Pharmacy
Sarah E. Corcoran, Reena Halai, Matthew A. Cooper
Summary: Activation of the NLRP3 inflammasome can lead to inflammatory responses and cell death, and is associated with various diseases. Inhibitors of NLRP3 have potential clinical value in preventing the harmful effects of NLRP3 activation on human diseases.
PHARMACOLOGICAL REVIEWS
(2021)
Article
Materials Science, Biomaterials
Chee Wei Ang, Lendl Tan, Zhi Qu, Nicholas P. West, Matthew A. Cooper, Amirali Popat, Mark A. T. Blaskovich
Summary: Mesoporous silica nanoparticles, particularly amino-functionalized MCM-41, show promise as carriers for improving the solubility and pharmacological activity of poorly soluble antitubercular agents such as Pretomanid and MCC7433. These nanoparticles were able to enhance the systemic exposure of MCC7433 in mice, suggesting their potential for oral delivery of tuberculosis treatments.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Microbiology
Marwa M. Hassan, Lucy F. Grist, Aurore C. Poirier, Roberto M. La Ragione
Summary: LAMP is a rapid alternative to PCR, allowing the reaction to occur at a single temperature using a polymerase with high displacement activity. Its advantages include the ability to rapidly detect nucleic acid targets within 30 minutes and its ease of use, making it suitable for various diagnostic settings.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Marwa M. Hassan, Arnoud H. M. van Vliet, Owen Higgins, Liam P. Burke, Alexandra Chueiri, Louise O'Connor, Dearbhaile Morris, Terry J. Smith, Roberto M. La Ragione
Summary: Environmental water is a major route for the transmission of antimicrobial resistance (AMR) and poses a growing threat to human and animal health. This study used comparative genomics to identify the prevalence of AMR genes in several bacteria and developed a rapid and sensitive method for detecting these genes in water samples. The developed assays successfully detected five AMR gene markers within 1 hour and can be easily implemented in resource-limited areas to enhance AMR surveillance and diagnostics.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Microbiology
Owen Higgins, Alexandra Chueiri, Louise O'Connor, Sinead Lahiff, Liam Burke, Dearbhaile Morris, Nicola Maria Pfeifer, Belen Gonzalez Santamarina, Christian Berens, Christian Menge, Manuela Canica, Vera Manageiro, Veljo Kisand, Marwa M. M. Hassan, Brian Gardner, Arnoud H. M. van Vliet, Roberto M. La Ragione, Bruno Gonzalez-Zorn, Terry J. J. Smith
Summary: CTX-M ESBL-producing E. coli is an increasing public health concern, and accurate identification of CTX-M variants is crucial for disease monitoring and treatment. CTX-M-1 and CTX-M-15 are the main ESBL-producing Enterobacteriaceae associated with animal and human infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Computer Science, Information Systems
Hardik A. Jain, Vinti Agarwal, Chaarvi Bansal, Anupama Kumar, Faheem FaheemFaheem, Muzaffar-Ur-Rehman Mohammed, Sankaranarayanan Murugesan, Moana M. Simpson, Avinash V. Karpe, Rohitash Chandra, Christopher A. MacRaild, Ian K. Styles, Amanda L. Peterson, Matthew A. Cooper, Carl M. J. Kirkpatrick, Rohan M. Shah, Enzo A. Palombo, Natalie L. Trevaskis, Darren J. Creek, Seshadri S. Vasan
Summary: Repurposing approved drugs is an excellent strategy to quickly obtain safe and effective COVID-19 treatments. To address this, the CoviRx.org platform provides a user-friendly interface for analyzing and filtering large quantities of data, while encouraging users to submit their findings. This will help combat the pandemic and expand to other diseases.
Article
Biochemistry & Molecular Biology
Ramar Perumal Samy, Stephen P. P. Mackessy, Alagarmalai Jeyasankar, Mano Ranjana Ponraj, Octavio Luiz Franco, Matthew A. A. Cooper, Matheswaran Kandasamy, Tapan Kumar Mohanta, Jebasingh Bhagavathsingh, Sakthivel Vaiyapuri
Summary: PaTx-II, an active component from the venom of the Mulga Snake, showed potent antimicrobial activity and promoted wound healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Bing Zhang, Wanida Phetsang, M. Rhia L. Stone, Sanjaya Kc, Mark S. S. Butler, Matthew A. A. Cooper, Alysha G. G. Elliott, Urszula Lapinska, Margaritis Voliotis, Krasimira Tsaneva-Atanasova, Stefano Pagliara, Mark A. T. Blaskovich
Summary: Antimicrobial resistance is a critical concern for human health, and this study presents the development of vancomycin fluorescent probes that can be used for detecting and visualizing Gram-positive bacteria as well as for measuring outer membrane permeabilization in Gram-negative bacteria. The probes were synthesized by modifying vancomycin with an azide substituent, enabling it to undergo copper-catalyzed azide-alkyne cycloaddition reactions. The probes exhibited similar antibacterial profiles to the parent vancomycin antibiotic and were versatile tools for various detection and imaging techniques.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Esther Sweeney, Niamh E. Harrington, Alicia G. Harley Henriques, Marwa M. Hassan, Branagh Crealock-Ashurst, Alan R. Smyth, Matthew N. Hurley, Maria Angeles Tormo-Mas, Freya Harrison
Summary: Research shows that Staphylococcus aureus demonstrates significant clinical characteristics in an ex vivo model of CF infection, including colonization of the airway lumen, localization as multicellular aggregates in mucus, initiation of a small colony variant phenotype, and increased antibiotic tolerance of tissue-associated aggregates, without tissue invasion or abscess formation.
Article
Biochemistry & Molecular Biology
M. Rhia L. Stone, Urszula Lapinska, Stefano Pagliara, Muriel Masi, Joanne T. Blanchfield, Matthew A. Cooper, Mark A. T. Blaskovich
RSC CHEMICAL BIOLOGY
(2020)
Article
Chemistry, Medicinal
Chee Wei Ang, Lendl Tan, Melissa L. Sykes, Neda AbuGharbiyeh, Anjan Debnath, Janet C. Reid, Nicholas P. West, Vicky M. Avery, Matthew A. Cooper, Mark A. T. Blaskovich
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)