Article
Biochemistry & Molecular Biology
Adam Krzystyniak, Agata Gluchowska, Grazyna Mosieniak, Ewa Sikora
Summary: Cell senescence, associated with aging and chronic diseases, can be detected by the activity of SA-beta-gal. To overcome the limitations of manual counting, we developed a Fiji-based macro extension that automatically and unbiasedly analyzes the integrated density of SA-beta-gal signal. Our tool is faster and provides higher resolution than manual methods, making it valuable in senescence research.
Article
Hematology
Arthur Flohr Svendsen, Daozheng Yang, KyungMok Kim, Seka Lazare, Natalia Skinder, Erik Zwart, Anna Mura-Meszaros, Albertina Ausema, Bjoern von Eyss, Gerald de Haan, Leonid Bystrykh
Summary: Despite heterogeneity between individual studies, a core and robust signature of transcriptional deregulation in aging murine hematopoietic stem cells (HSC) was identified, characterized by increased transcriptional activation and membrane-associated transcripts. Additionally, the presence of transcriptionally young-like HSCs in aged bone marrow was revealed through single-cell transcriptomics analysis.
Article
Multidisciplinary Sciences
Qing Zhang, Xiao Lei, Fang Wang, Xiaoning He, Lu Liu, Yuxia Hou, Yuan Liu, Fang Jin, Chider Chen, Bei Li
Summary: Immune system disorders, especially T cell disorders, are important therapeutic targets of mesenchymal stem cells (MSCs) in many autoimmune diseases (ADs). ERK1 plays a crucial role in regulating T cells in MSCs, and MSCs treated with LA can improve the efficacy of MSC-based treatments for ADs.
Article
Cell Biology
Jose Antonio de Mera-Rodriguez, Guadalupe Alvarez-Hernan, Yolanda Ganan, Ana Santos-Almeida, Gervasio Martin-Partido, Joaquin Rodriguez-Leon, Javier Francisco-Morcillo
Summary: The histochemical detection of beta-galactosidase enzymatic activity in the developing postnatal olfactory epithelium in mice suggests a correlation with olfactory marker protein, indicating a potential link to neuronal differentiation rather than cellular senescence.
Article
Endocrinology & Metabolism
Sneha S. Varghese, Sangeeta Dhawan
Summary: Cellular senescence is a complex process that results in permanent cell-cycle arrest in response to various stressors. It plays a crucial role in aging, disease development, embryogenesis, tissue regeneration, and tissue repair. Senescence is particularly important in pancreatic beta-cells, where it contributes to the decline in regenerative capacity and failure in diabetes. Understanding the molecular mechanisms of beta-cell senescence and its role in growth and development is significant for potential therapeutic interventions.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chung-Da Yang, Shu-Chun Chuang, Tsung-Lin Cheng, Mon-Juan Lee, Hui-Ting Chen, Sung-Yen Lin, Hsuan-Ti Huang, Cheng-Jung Ho, Yi-Shan Lin, Lin Kang, Mei-Ling Ho, Je-Ken Chang, Chung-Hwan Chen
Summary: The study introduces a new culture formula for human bone marrow stem cells, which allows for maintaining and expanding the cells with high proliferation rate and multiple lineage differentiation ability. The new formula also retains stable chromosome, DNA, telomere length, and telomerase activity of the cells even after long-term culture, minimizing senescence and preventing carcinogenesis. These modifications enhance the survival rate, growth rate, and basal characteristics of isolated HBMSCs, providing valuable insights for stem cell research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jinwoo Nah, Rho H. Seong
Summary: This study identifies KLF4 as a key regulator of CD8 T cell exhaustion and cytolytic effector function, promoting the differentiation of transient effector CD8 T cells and enhancing antitumor immunity. High KLF4 expression is positively correlated with a favorable prognosis in human cancer patients.
Review
Biochemistry & Molecular Biology
Qiang Zong, Katrin Bundkirchen, Claudia Neunaber, Sandra Noack
Summary: Bone marrow-derived mesenchymal stem cells (BMSCs) have potential for cell-based therapies, but their properties can be affected by the overweight/obesity microenvironment. This review summarizes evidence on the effects of overweight/obesity on BMSC characteristics. Existing studies show inconsistent results and the mechanisms involved are still unclear. Further research is needed to improve the functions of overweight/obesity-derived BMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Jin-Ran Chen, Oxana P. Lazarenko, Michael L. Blackburn, Jennifer F. Chen, Christopher E. Randolph, Jovanny Zabaleta, Katrin Schroder, Kim B. Pedersen, Martin J. J. Ronis
Summary: Nox4 expression and reactive oxygen species signaling play a key role in osteoblast differentiation, proliferation, and maturation. Nox4 expression and ROS signaling in bone and osteoblastic cells coordinately contribute to osteoblast differentiation, proliferation, and maturation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mei Ge, Xuehan Bai, Aoyi Liu, Lingjuan Liu, Jie Tian, Tiewei Lu
Summary: Left ventricular noncompaction (LVNC) is a heterogeneous disorder with unclear genetic causes, and eIF3a gene mutation may contribute to LVNC development by affecting processes such as cell proliferation, apoptosis, migration, and differentiation. This study revealed that the eIF3a gene mutation may disrupt the ERK1/2 pathway leading to pathological changes in LVNC.
Article
Physiology
Fan Yang, Elyce Ozols, Frank Y. Ma, Khai Gene Leong, Greg H. Tesch, Xiaoyun Jiang, David J. Nikolic-Paterson
Summary: The JNK signaling pathway plays a role in acute high dose AA-induced kidney injury, but is not essential in promoting chronic kidney disease caused by ongoing DNA damage from chronic low dose AA exposure.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Geriatrics & Gerontology
An Yu, Ruofan Yu, Haiying Liu, Chenliang Ge, Weiwei Dang
Summary: Adipose tissue is an important endocrine organ that regulates metabolism, immune response, and aging in mammals. SIRT1 is a crucial regulator that negatively regulates adipogenic differentiation. Knocking out SIRT1 in mesenchymal stem cells causes defects in osteogenesis and loss of adipose tissue. Impairment of SIRT1 function in MSCs during adipogenic differentiation leads to significant defects and cellular senescence.
Article
Cell Biology
Arnaud Delpoux, Nimi Marcel, Rodrigo Hess Michelini, Carol D. Katayama, Karmel A. Allison, Christopher K. Glass, Sergio M. Quinones-Parra, Cornelis Murre, Liyen Loh, Katherine Kedzierska, Martha Lappas, Stephen M. Hedrick, Andrew L. Doedens
Summary: This study demonstrates the crucial role of FOXO1 in maintaining cellular vitality and response to antigenic stress in T cells. Different cellular age states may lead to alterations in levels of FOXO1, TCF7, and AP-1 factors. These characteristics are also associated with the formation of T cells with features of cellular senescence.
Article
Multidisciplinary Sciences
Jingjing Wang, Maureen Mongan, Xiang Zhang, Ying Xia
Summary: The study developed sustainable mouse keratinocyte lines that exhibited different characteristics under different culture conditions, providing important resources for skin research. The cells derived in low-Ca2+ medium showed enriched stem/proliferation markers, while the cells adapted in Defined-KSFM media had epithelial progenitor signatures and could differentiate to suprabasal epithelial cell types in 3D organoids.
Article
Cell Biology
Luiza Steffens Reinhardt, Kira Groen, Xiajie Zhang, Brianna C. Morten, Anna Wawruszak, Kelly A. Avery-Kiejda
Summary: In breast cancer, dysregulated TP53 expression signatures are more accurate predictors of chemotherapy response and survival outcomes than TP53 mutations. High levels of & UDelta;40p53 are associated with worse disease-free survival and disruption of p53-induced DNA damage response. We found that genes related to cell differentiation were downregulated, while those associated with stem cell regulation were upregulated in invasive ductal carcinomas expressing high levels of & UDelta;40p53. Furthermore, high levels of & UDelta;40p53 induce tumor growth, promote chemoresistance, and enhance the stemness phenotype in breast cancer. Targeting tumors with a high & UDelta;40p53:p53 ratio could improve the efficacy of standard-of-care therapies.
CELL DEATH & DISEASE
(2023)
Review
Cell & Tissue Engineering
Yuliya Safarova, Bauyrzhan Umbayev, Gonzalo Hortelano, Sholpan Askarova
REGENERATIVE MEDICINE
(2020)
Review
Nutrition & Dietetics
Assylzhan Yessenkyzy, Timur Saliev, Marina Zhanaliyeva, Abdul-Razak Masoud, Bauyrzhan Umbayev, Shynggys Sergazy, Elena Krivykh, Alexander Gulyayev, Talgat Nurgozhin
Review
Immunology
Sholpan Askarova, Bauyrzhan Umbayev, Abdul-Razak Masoud, Aiym Kaiyrlykyzy, Yuliya Safarova, Andrey Tsoy, Farkhad Olzhayev, Almagul Kushugulova
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Review
Cell Biology
Bauyrzhan Umbayev, Sholpan Askarova, Aigul Almabayeva, Timur Saliev, Abdul-Razak Masoud, Denis Bulanin
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2020)
Article
Biochemistry & Molecular Biology
Tulendy Nurkenov, Andrey Tsoy, Farkhad Olzhayev, Elvira Abzhanova, Anel Turgambayeva, Aizhan Zhussupova, Bharathi Avula, Samir Ross, Aigerim Aituarova, Dariya Kassymova, Galiya Zhusupova, Tamara Shalakhmetova, Tursonjan Tokay, James C. Lee, Sholpan Askarova
Summary: Evidence suggests that plant polyphenols, particularly those from L. gmelinii roots, have neuroprotective effects by reducing oxidative stress and inflammatory responses in the brain. Experiments conducted in vitro and in vivo demonstrated that the plant extract suppressed oxidative stress and pro-inflammatory reactions in neurons, astrocytes, and cerebral endothelial cells. Additionally, treatment with the L. gmelinii extract improved motor function in rats with middle cerebral artery occlusion.
Meeting Abstract
Biochemistry & Molecular Biology
Abdul Masoud, David K. Mills
Article
Multidisciplinary Sciences
Aiym Kaiyrlykyzy, Samat Kozhakhmetov, Dmitriy Babenko, Gulnaz Zholdasbekova, Dinara Alzhanova, Farkhad Olzhayev, Aida Baibulatova, Almagul R. Kushugulova, Sholpan Askarova
Summary: This study investigated the diversity and composition of gut microbiota in AD patients and healthy seniors from Kazakhstan. The results showed substantial differences in bacterial abundance at various taxonomic levels between AD patients and healthy individuals.
SCIENTIFIC REPORTS
(2022)
Review
Geriatrics & Gerontology
Bauyrzhan Umbayev, Yuliya Safarova (Yantsen), Aislu Yermekova, Assem Nessipbekova, Aizhan Syzdykova, Sholpan Askarova
Summary: Cdc42 is an important member of the Rho family, involved in various cellular functions and critical for the development of aging-related pathologies. Dysregulation of Cdc42 has been implicated in cellular and tissue aging. However, its expression and activity are complex and regulated by multiple factors.
Article
Genetics & Heredity
Aiym Kaiyrlykyzy, Bauyrzhan Umbayev, Abdul-Razak Masoud, Aida Baibulatova, Andrey Tsoy, Farkhad Olzhayev, Dinara Alzhanova, Gulnaz Zholdasbekova, Kairat Davletov, Ainur Akilzhanova, Sholpan Askarova
Summary: This study investigated the levels of adiponectin, methylation of the adiponectin gene promoter, and expression of adiponectin receptors in blood samples from Alzheimer's disease (AD) patients and healthy controls. The results showed higher levels of adiponectin in AD patients, with a positive correlation between adiponectin and MMSE scores and high-density lipoprotein cholesterol. The study also found differences in methylation of the adiponectin gene promoter and higher levels of AdipoR1 in AD patients. These findings contribute to a better understanding of the factors contributing to dementia and the development of predictive biomarkers for AD.
BMC MEDICAL GENOMICS
(2022)
Review
Materials Science, Multidisciplinary
Abdul-Razak Masoud, Femi Alakija, Mohammad Jabed Perves Bappy, Patrick A. S. Mills, David K. Mills
Summary: Halloysite nanotubes (HNTs) are versatile nanoparticles with applications in polymer reinforcement, drug release, synthesis, material removal, medicine, and coatings. This review focuses on metallizing the HNT surfaces and explores the use of metal-coated HNTs in nanomedicine. The potential applications of metal-coated HNTs in anti-infective therapy, immunotherapy, dentistry, regenerative medicine, and wound healing are discussed. The importance of HNTs in aerospace, defense, industry, and the market potential of metal oxide nanoparticles is also highlighted. The commercialization potential of metal-coated HNTs is evaluated.
Review
Materials Science, Composites
Mohammadmahdi Mobaraki, Meichen Liu, Abdul-Razak Masoud, David K. Mills
Summary: Human tissues and disease models require biomimetic microenvironments. Solution blow spinning is a facile method for fabricating nanofibers and films, with various applications in areas such as tissue engineering and wound healing. This review discusses fiber fabrication methods and explores the potential of blow-spinning in biomedical applications.
JOURNAL OF COMPOSITES SCIENCE
(2023)
Article
Oncology
D. Alimbetov, B. Umbayev, A. Tsoy, D. Begimbetova, T. Davis, D. Kipling, Sh. Askarova
Summary: The study investigates the potential of using small molecule inhibitors of p38MAPK/MK2 stress signaling pathways to enhance the sensitivity of cancer cells with abrogated G1 checkpoint to the DNA damaging agent etoposide.
Article
Biotechnology & Applied Microbiology
Yuliya Safarova Yantsen, Farkhad Olzhayev, Bauyrzhan Umbayev, Andrey Tsoy, Gonzalo Hortelano, Tursonjan Tokay, Hironobu Murata, Alan Russell, Sholpan Askarova
BIOENGINEERING-BASEL
(2020)