Article
Biotechnology & Applied Microbiology
Anna Ylinen, Jorg C. de Ruijter, Paula Jouhten, Merja Penttila
Summary: This study demonstrated the production of poly(hydroxybutyrate) (PHB) in yeast Saccharomyces cerevisiae using cellobiose as a sole carbon source. Utilizing different cellobiose utilization routes led to increased PHB production and accumulation percentage, offering a promising solution for sustainable polymer production using microbial cells.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Tao Sheng, Qingbin Meng, Zhiling Li, Caiyu Sun, Lixin Li, Lilai Liu
Summary: This study investigates the mechanism of cellulose degradation in strain M3 and reveals the presence of unique beta-glucosidases and related proteins in R. thermocellum M3 compared to other strains. These proteins contribute to improved cellulose hydrolysis efficiency. Additionally, key genes involved in eliminating feedback inhibition of cellobiose in R. thermocellum are predicted.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Zedong Jiang, Liufei Long, Meifang Liang, Hebin Li, Yanhong Chen, Mingjing Zheng, Hui Ni, Qingbiao Li, Yanbing Zhu
Summary: In this study, a new beta-glucosidase MaGlu1A was cloned from a marine bacterium, showing optimal activity at 40 degrees C and pH 4.5, substrate preference for aryl-beta-glycosidic bonds with glucose, fucose, and galactose moieties, and strong stimulation by supplemental glucose. The enzymatic characterization of MaGlu1A provides valuable information for its practical applications.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Wei Xia, Yingguo Bai, Pengjun Shi
Summary: By analyzing and designing the active center of Bgl3A, this study found that enhancing hydrophobic interactions and hydrogen-bonding networks can strengthen substrate binding, resulting in four dominant mutants with significantly lower K-m values and higher catalytic efficiencies. These findings may serve as a valuable reference for the design of other beta-glucosidases and glycoside hydrolases.
Article
Nanoscience & Nanotechnology
Yang Zhao, Mingzhao Yang, Man Li, Hongsheng Dong, Yang Ge, Qingping Li, Lunxiang Zhang, Yu Liu, Lei Yang, Yongchen Song, Jiafei Zhao
Summary: Efficient gas enrichment methods are crucial for the storage, transportation, and sequestration of clean energy and carbon dioxide. A novel recyclable hydrate promoter has been developed, which effectively reduces the induction time of methane hydrate formation, increases gas storage capacity, and inhibits foam during hydrate decomposition. Additionally, this promoter enhances CO2 storage capacity and shows potential for sequestering CO2 in the form of gas hydrate under the seafloor.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Anica Dadwal, Vishal Singh, Shilpa Sharma, Tulasi Satyanarayana
Summary: This study analyzed the structure of a cellulase enzyme from a thermophilic fungus using computational methods. The study provided insights into the structural characteristics of the enzyme and predicted its binding sites and tolerance to different sugars.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Chemistry, Applied
Xin Li, Danlin Zeng, Zhiyong He, Ping Ke, Yongsheng Tian, Guanghui Wang
Summary: Efficient and recyclable magnetic chitosan microspheres (MCMs) were synthesized for removing iodide from nuclear wastewater. MCMs demonstrated high removal potential for iodide anions under different conditions and showed good regeneration and reusability.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Environmental
Hyeongoo Kim, Gang Zhang, Min Wu, Jinshan Guo, Changwoo Nam
Summary: A novel flake type polyolefin-based magnetic absorbent (PMA) hybridized with magnetic nanoparticle has been developed for oil spill cleanup, showing convenient collection, efficient oil absorption, and effective recycling capabilities.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Yang Zhao, Yanzhen Liu, Hongsheng Dong, Chong Chen, Tianxiang Zhang, Lei Yang, Lunxiang Zhang, Yu Liu, Yongchen Song, Jiafei Zhao
Summary: This paper proposes a new method for coating inhibitors, which allows for the magnetic recovery of inhibitory particles and demonstrates good cycle performance. The experimental results show that this method can effectively extend the formation time of hydrates and solve pipeline blockage issues by preventing further growth of hydrates.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Rafael Leandro Fernandes Melo, Misael Bessa Sales, Viviane de Castro Bizerra, Paulo Goncalves de Sousa Junior, Antonio Luthierre Gama Cavalcante, Tiago Melo Freire, Francisco Simao Neto, Muhammad Bilal, Teofil Jesionowski, Joao Maria Soares, Pierre Basilio Almeida Fechine, Jose Cleiton Sousa dos Santos
Summary: Magnetic biocatalysts, combining magnetic properties and enzyme catalytic activity, offer easy recovery and reuse. Lipases immobilized by magnetic nanoparticles dominate research in this field. This review provides a bibliometric analysis and overview of the area, highlighting research advances and hotspots, as well as the diverse applications of magnetic biocatalysts.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biophysics
Wenrui Huang, Wei Zheng, Juan Han, Jiacong Wu, Yuanyuan Li, Yanli Mao, Lei Wang, Yun Wang
Summary: In this study, UCST-responsive polymers were synthesized and used to immobilize beta-glucosidase, resulting in significantly improved temperature, pH, storage, and operation stabilities. The one-step immobilization of crude enzyme solution can save purification steps and reduce experimental costs, showing potential for practical green biocatalysis.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biotechnology & Applied Microbiology
Girija Kaushal, Amit K. Rai, Sudhir P. Singh
Summary: A novel beta-glucosidase gene, bgl(M), was identified through computational screening of an extreme temperature aquatic habitat metagenomic resource. The expressed and purified enzyme, Bgl(M), exhibited significant hydrolytic potential towards cellobiose and lactose, showing stable activity in a wide range of pH and temperature. It also displayed high tolerance to glucose and ethanol, making it a promising enzyme for cellulose saccharification and enhancement of nutritional values in soya and wine industries.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Review
Agricultural Engineering
Jon Del Arco, Andres R. Alcantara, Roberto Fernandez-Lafuente, Jesus Fernandez-Lucas
Summary: The use of magnetic biocatalysts simplifies operational processing, increases efficiency, and reduces costs. Small-size matrices as carriers for enzyme immobilization help maximize surface area and catalysts loading, while reducing diffusion limitations.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Chemical
Clara Yepes, Juliana Estevez, Miguel Arroyo, Miguel Ladero
Summary: In this study, various covalent immobilization methods were used to immobilize a commercial cocktail with high beta-glucosidase activity. The immobilized enzyme showed higher activity at high enzyme loadings and stability similar to the free enzyme. A kinetic model based on the Michaelis-Menten hypothesis was selected and compared to the model for the free enzyme, indicating different kinetic properties of the immobilized enzyme.
Article
Chemistry, Multidisciplinary
Shuheng Wei, Bo Liu, Xiaonan Huang, Yongkang Xi, Shuxin Wang, Like Wang, Shouwei Yin, Xiaoquan Yang
Summary: CO2/N-2 responsive sodium caseinate-enzyme conjugates were designed as catalytic sites and stabilizers for Pickering interfacial biocatalysis, resulting in improved enzyme stability and catalytic efficiency in harsh environments.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)