Article
Chemistry, Multidisciplinary
Thien-Kim Le, Jinhyun Kim, Ngoc Anh Nguyen, Thi Huong Ha Nguyen, Eun-Gene Sun, Su-Min Yee, Hyung-Sik Kang, Soo-Jin Yeom, Chan Beum Park, Chul-Ho Yun
Summary: Photobiocatalysis is a green platform for driving redox enzymatic reactions using solar energy without high-cost cofactors. Natural flavins were used as photosensitizers to develop a visible light-driven whole-cell platform for human cytochrome P450 (CYP) photobiocatalysis. The research showed that flavins can mediate light-driven biocatalysis by human CYPs in whole-cell systems, demonstrating the general applicability of solar-powered P450 photobiocatalytic system.
Article
Chemistry, Physical
Christian R. Zwick III, Hans Renata
Summary: Modified amino acids play crucial roles in natural biomolecules and synthetic small-molecule drugs. This review focuses on the roles of iron-and alpha-ketoglutarate-dependent dioxygenases in the oxidative modification of amino acids, particularly C-H hydroxylation and halogenation. Recent advances in engineering approaches, such as altering substrate specificity and improving catalytic efficiency, are also discussed, along with their applications in complex molecule synthesis. The review concludes with a brief discussion on the discovery of unique oxidative cyclization activities by certain family members and the challenges in expanding the biocatalytic utility and reaction scope of the enzyme family.
Review
Chemistry, Multidisciplinary
Hossay Abas, Peter Blencowe, Joanna L. Brookfield, Lucy A. Harwood
Summary: Steroids are common structures in small-molecule therapeutics, and their oxidation level is crucial for their biological activity and physicochemical properties. These tetracyclic structures with multiple stereocenters play a vital role in creating specific vectors and protein binding orientations. Therefore, the ability to selectively hydroxylate steroids is essential for researchers in this field. This review will discuss three main methods for hydroxylation: biocatalysis, metal-catalyzed C-H hydroxylation, and organic oxidants such as dioxiranes and oxaziridines.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Kun Zhang, Aiqin Yu, Xuan Chu, Fudong Li, Juan Liu, Lin Liu, Wen-Ju Bai, Chao He, Xiqing Wang
Summary: Selective hydroxylation of non-activated aliphatic C-H bonds was achieved through directed evolution of P450(BS beta) hydroxylase. The reaction showed high selectivity towards various aliphatic carboxylic acids with different chain lengths, functional groups, and aromatic moieties. The X-ray structure of the evolved enzyme variant provided insights into the regio- and enantioselectivity of the reaction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Jeffrey Bruffaerts, Inbar Kesten, Keren Buhnik-Rosenblau, Anthony Cohen, Nurit Edri, Morgan Cormier, Yuanfei Zhang, Guo-Ming Ho, Itai Massad, Hila Halfon-Verner, Yechezkel Kashi, Ilan Marek
Summary: A two-step sequential strategy involving a biocatalytic dehydrogenation/remote hydrofunctionalization is reported for selectively converting linear alkanes into valuable functionalized aliphatic derivatives. The dehydrogenation is conducted by a mutant strain of Rhodococcus bacteria, and the resulting alkenes are further functionalized through a metal-catalyzed hydrometalation/migration sequence with various electrophiles. This combined biocatalytic and organometallic approach enables high-yielding site-selective functionalization of unreactive primary C-H bonds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Joshua M. Paolillo, Alana D. Duke, Emma S. Gogarnoiu, Dan E. Wise, Marvin Parasram
Summary: A photoexcited-nitroarene-mediated anaerobic C-H hydroxylation of aliphatic systems was reported. The success of this reaction was attributed to the dual functionality of the photoexcited nitroarene as both the C-H bond activator and the oxygen atom source. Compared to previous methods, this approach is cost-effective and atom-economical due to the commercial availability of the nitroarene. The anaerobic conditions of the transformation allow for a significant expansion in substrate scope compared to prior reports. Mechanistic studies revealed the involvement of successive hydrogen atom transfer and radical recombination events, leading to N-arylhydroxylamine ether intermediates and key oxygen atom transfer products.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Elijah N. Kissman, Monica E. Neugebauer, Kiera H. Sumida, Cameron Swenson, Nicholas A. Sambold, Jorge A. Marchand, Douglas C. Millar, Michelle C. Y. Chang
Summary: This study investigates the application of enzyme-catalyzed C-H activation in selective halogenation, and elucidates the crucial role of substrate-binding lid in selective halogenation through crystal structures of HalB and HalD, providing guidance for the development of halogenases with biocatalytic applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Florian Leisinger, Dzmitry A. Miarzlou, Florian P. Seebeck
Summary: Molecular oxygen is a sustainable oxidation reagent with strong oxidizing properties and stability, ultimately forming water as the reaction product. The activation of O-2 can occur through various pathways, with enzymes typically forming metal-oxygen coordination complexes to facilitate the process. The formylglycine generating enzyme (FGE) demonstrates a unique strategy for activating O-2, involving binding of O-2 near, but not coordinated to, its catalytic copper atom.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Mario A. Cribari, Maxwell J. Unger, Jeffrey D. Martell
Summary: Enzyme-mediator systems, such as the horseradish peroxidase (HRP), H2O2, and N-hydroxyphthalimide (NHPI) system, are effective for the functionalization of alkylbenzenes. The HRP-NHPI system shows superior activity compared to existing enzyme-mediator systems, converting alkylbenzenes to ketones and aldehydes. This system operates under mild conditions and can be used in aqueous-organic solvent mixtures. It also enables the conversion of alkylbenzenes to benzylic amines in a one-pot, two-step enzymatic cascade.
Article
Chemistry, Inorganic & Nuclear
Roman V. Ottenbacher, Alexander G. Medvedev, Andrey A. Nefedov, Konstantin P. Bryliakov
Summary: In the past decade, manganese complexes with bis-amino-bis-pyridylmethyl and related N4 donor ligands have been found to be efficient catalysts for selective epoxidations and C-H oxidations using H2O2. However, the use of (quinolin-2-yl)methyl groups in constructing such catalysts has not been explored. In this study, we report the crystal structures of several Mn complexes with (quinolin-2-yl)methyl and discuss the possible reasons for their inactivity in oxidation catalysis.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Nagaraju Mupparapu, Lauren Brewster, Katrina F. Ostrom, Sherif I. Elshahawi
Summary: A method for late-stage functionalization of indole- and tryptophan-containing compounds with reactive moieties to alter their biological properties was studied. Two hydroxy-bearing allyl pyrophosphates were synthesized. A chemo-enzymatic approach using a promiscuous indole prenyltransferase enzyme was demonstrated, introducing a dual reactive hydroxy-bearing allyl moiety directly onto the indole ring of tryptophan-containing peptides. This is the first report of late-stage indole modifications with this reactive group.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Alexander Swoboda, Lukas Johannes Pfeifenberger, Zerina Duhovic, Moritz Buergler, Isabel Oroz-Guinea, Klara Bangert, Florian Weissensteiner, Lena Parigger, Katharina Ebner, Anton Glieder, Wolfgang Kroutil
Summary: A high-throughput, stereoselective screening assay is reported for identifying peroxygenases that display high enantio-/stereoselectivity in hydroxylation reactions. The assay provides information on enantioselectivity, stereopreference, and activity and was used to identify previously non-described wild-type peroxygenases with (S)- and (R)-stereoselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Multidisciplinary
Maoyao Wang, Xiaojian Zhou, Zhongqiang Wang, Yongzheng Chen
Summary: Chiral allylic oxidized products play a significant role in the pharmaceutical, agrochemical, and pharmaceutical industries. Biocatalytic C-H oxyfunctionalization serves as a simplified synthesis method. New enzymes and mutants have been discovered through techniques of gene mining and enzyme-directed evolution.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Physical
Ke Zheng, Song Shi, Hongchuan Xin, Jin Gao, Zengjian An
Summary: A method for selectively activating the C-H bond of phenol into a C-OH bond was developed using Cu2+ ions and hydrogen peroxide in a two-phase system. Addition of alcohols increased the selectivity for catechol in the product. This offers an effective hydroxylation method for producing catechol from phenol.
CATALYSIS COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Zuzanna Wojdyla, Tomasz Borowski
Summary: Clavaminic acid synthase is a crucial Fe-II/2-oxoglutarate-dependent dioxygenase from Streptomyces clavuligerus, essential for biosynthesis of the beta-lactamase inhibitor clavulanic acid. The enzyme's versatility and selectivity depend on the binding cavity, which interacts differently with substrates for various reactions. Enzyme-substrate interactions affect substrate re-positioning during reactions, with differential effects on hydroxylation and desaturation due to specific residues in the binding cavity.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Deepankar Gahloth, Karl Fisher, Karl A. P. Payne, Matthew Cliff, Colin Levy, David Leys
Summary: This study provides structural and biochemical insights into the indole-3-carboxylic acid decarboxylase, a member of the UbiD enzyme family. The enzyme likely undergoes an open-closed transition as a consequence of domain motion, which is coupled to catalysis. The study also demonstrates the coupling of indole-3-carboxylic acid decarboxylase with carboxylic acid reductase to produce indole-3-carboxyaldehyde from indole + CO2 under ambient conditions, providing evidence for a common mode of action in the UbiD enzyme family.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Editorial Material
Chemistry, Applied
Carlos A. Martinez, Nicholas J. Turner, Zhi Li
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Article
Chemistry, Multidisciplinary
Johannes Buchler, Eimear Hegarty, Kirsten Schroer, Radka Snajdrova, Nicholas J. J. Turner, Olivier Loiseleur, Rebecca Buller, Camille Le Chapelain
Summary: Novartis AG and Syngenta AG collaborated with the Competence Center for Biocatalysis (CCBIO) to develop a sustainable approach to late-stage halogenation using machine-learning guided protein engineering. They successfully evolved versatile catalysts capable of selectively chlorinating complex molecules, demonstrating the synthetic usefulness of such a strategy.
HELVETICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Fei-Fei Chen, Xue-Feng He, Xin-Xin Zhu, Zhi Zhang, Xin-Yuan Shen, Qi Chen, Jian-He Xu, Nicholas J. Turner, Gao-Wei Zheng
Summary: The direct synthesis of a wide range of sterically demanding secondary amines using imine reductases has been achieved, enabling the production of important pharmaceutical ingredients and intermediates.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Physical
Amelia K. Gilio, Thomas W. Thorpe, Alex Heyam, Mark R. Petchey, Balazs Pogranyi, Scott P. France, Roger M. Howard, Michael J. Karmilowicz, Russell Lewis, Nicholas Turner, Gideon Grogan
Summary: Imine reductases (IREDs) can catalyze the reduction of cyclic imines and the coupling of ketones and amines to form secondary amine products. The larger amines used in the coupling reactions were likely recruited from solution for enzyme reduction. Mutant IR77-A208N showed improved activity for amine product formation.
Article
Chemistry, Physical
Christian Schnepel, Laura Rodriguez Perez, Yuqi Yu, Antonio Angelastro, Rachel S. Heath, Max Lubberink, Francesco Falcioni, Keith Mulholland, Martin A. Hayes, Nicholas J. Turner, Sabine L. Flitsch
Summary: The activation of carboxylic acids to thioesters, especially Coenzyme A-based ones, is important but challenging. A generic thioester recycling enzyme, CARsr-A, was developed to catalyze the conversion of carboxylic acids to thioesters. This recycling system can regenerate thioesters for various acyl-S-Coenzyme A-dependent enzymes and enable the formation of diverse amides and non-native acylation reactions.
Article
Chemistry, Physical
Ashleigh J. Burke, Thomas M. Lister, James R. Marshall, Murray J. B. Brown, Richard Lloyd, Anthony P. Green, Nicholas J. Turner
Summary: Reductive aminases (RedAms) have shown potential in catalyzing coupling reactions between primary amines and aldehydes/ketones, leading to the synthesis of chiral secondary amines. However, the synthesis of tertiary amines, especially from coupling ketones with secondary amines, has been challenging. In this study, we demonstrated that RedAms, specifically RedAm-361 engineered through directed evolution, can selectively catalyze reductive aminations of cyclic secondary amines with carbonyl partners, including dynamic kinetic resolutions and enantioselective aminations. These biotransformations provide access to important structural motifs found in active pharmaceutical ingredients and other bioactive molecules. The discovery and engineering of RedAms have significant implications for the development of industrial biocatalysts in the production of key pharmaceutical intermediates.
Article
Chemistry, Multidisciplinary
Jack J. Sangster, Rebecca E. Ruscoe, Sebastian C. Cosgrove, Juan Mangas-Sanchez, Nicholas J. Turner
Summary: We present a one-pot chemoenzymatic process that combines monoamine oxidase (MAO-N)-catalyzed oxidation with metal-catalyzed allylboration and biocatalytic deracemization to efficiently synthesize enantioenriched C(1)-allylated tetrahydroisoquinolines. This cascade reaction is simple to operate, with all components added at the beginning of the reaction, and provides high yields and good to high enantiomeric excess of allylic amine derivatives, which can serve as important building blocks for further elaboration.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Physical
Haoyue Xiang, Salvatore Ferla, Carmine Varricchio, Andrea Brancale, Nicola L. Brown, Gary W. Black, Nicholas J. Turner, Daniele Castagnolo
Summary: Two enzymatic strategies were developed to oxidize 1,2,3,4-tetrahydroquinolines (THQs) and N-cyclopropyl-N-alkylanilines into quinolines and 2-quinolones, respectively. Whole cells and monoamine oxidase (MAO-N) enzymes were used for the biotransformation of THQs, while horseradish peroxidase (HRP)-catalyzed annulation/aromatization reaction followed by Fe-mediated oxidation was employed for the conversion of N-cyclopropyl-N-alkylanilines into 2-quinolone compounds.
Article
Multidisciplinary Sciences
Harshwardhan Poddar, Ronald Rios-Santacruz, Derren J. J. Heyes, Muralidharan Shanmugam, Adam Brookfield, Linus O. O. Johannissen, Colin W. W. Levy, Laura N. N. Jeffreys, Shaowei Zhang, Michiyo Sakuma, Jacques-Philippe Colletier, Sam Hay, Giorgio Schiro, Martin Weik, Nigel S. S. Scrutton, David Leys
Summary: This study provides insights into the mechanism of CarH, a photoreceptor involved in transcriptional regulation. The authors show that the response of CarH to light integrates both B-12 photochemistry and redox changes to drive large-scale conformational changes.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Yuqi Yu, Arnau Rue Casamajo, William Finnigan, Christian Schnepel, Rhys Barker, Charlotte Morrill, Rachel S. Heath, Leonardo De Maria, Nicholas J. Turner, Nigel S. Scrutton
Summary: Biocatalysis plays a crucial role in the discovery, development, and manufacturing of pharmaceuticals. A structure-based computational workflow called IREDFisher has been developed to prioritize protein sequences based on predicted activities, reducing the need for resource-intensive laboratory-based screening. IREDFisher successfully identified highly active imine reductases for various reactions, significantly reducing the number of samples that need to be tested in vitro. With a user-friendly web interface, IREDFisher enables rapid discovery of biocatalysts with minimal time and resource expenditure.
Article
Chemistry, Physical
Amelia K. Gilio, Thomas W. Thorpe, Alex Heyam, Mark R. Petchey, Balazs Pogranyi, Scott P. France, Roger M. Howard, Michael J. Karmilowicz, Russell Lewis, Nicholas Turner, Gideon Grogan
Summary: Imine reductases (IREDs) catalyze the reduction of cyclic imines and the coupling of ketones and amines, forming secondary amine products. IR77 enzyme was found to catalyze the coupling of larger bicyclic amines with cyclohexanone. Mutant IR77-A208N with improved activity for amine product formation was successfully synthesized using structure-guided mutagenesis.
Article
Chemistry, Multidisciplinary
Jack J. Sangster, Rebecca E. Ruscoe, Sebastian C. Cosgrove, Juan Mangas-Sanchez, Nicholas J. Turner
Summary: Here, we present a one-pot, chemoenzymatic process for synthesizing enantioenriched C(1)-allylated tetrahydroisoquinolines. This process involves the oxidation of monoamine oxidase (MAO-N) catalyst and the allylboration of a metal catalyst, followed by a biocatalytic deracemization, resulting in high yields and good to high enantiomeric excess of allylic amine derivatives. This cascade reaction is simple to operate, with all components added at the beginning of the reaction, and can produce key building blocks for further elaboration.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Physical
Amelia K. Gilio, Thomas W. Thorpe, Alex Heyam, Mark R. Petchey, Balazs Pogranyi, Scott P. France, Roger M. Howard, Michael J. Karmilowicz, Russell Lewis, Nicholas Turner, Gideon Grogan
Summary: Imine reductases (IREDs) can catalyze the reduction of cyclic imines and the coupling of ketones and amines to form secondary amine products. Previous studies mainly focused on using small hydrophobic amines, but for certain pharmaceutical targets, larger amines are required. In this study, the enzyme IR77 from Ensifer adhaerens was found to be a promising biocatalyst for the reductive amination of cyclohexanone with pyrrolidine. The mutant IR77-A208N showed improved activity for amine product formation, leading to isolated yields of up to 93% for the amination of cyclohexanone with larger amines.
Review
Chemistry, Multidisciplinary
Bo Yuan, Dameng Yang, Ge Qu, Nicholas J. Turner, Zhoutong Sun
Summary: This review summarizes the advancements in NAD(P)H-dependent amine dehydrogenases and imine reductases as biocatalysts for asymmetric reductive amination to produce optically active amines. These enzymes exhibit high activity and stereoselectivity, and can utilize a wide range of substrates. By discussing the evolutionary history, substrate scope, and applications of these enzymes, the review provides an outlook on emerging industrial biotechnologies for chiral amine production.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
