Article
Fisheries
Diana Bastos Almeida, Catarina Magalhaes, Zelia Sousa, Maria Teresa Borges, Eliane Silva, Isidro Blanquet, Ana Paula Mucha
Summary: Recirculating aquaculture systems (RAS) manage waste and nutrient recycling to make intensive fish production environmentally sustainable. This study investigated the dynamics of prokaryotic communities in relation to water parameters, finding that shifts in the community were modeled by factors such as salinity and pH. The research provides new knowledge essential for understanding microbial community balance in aquaculture and serves as a valuable tool for overall aquaculture system management.
Article
Environmental Sciences
Julia Clols-Fuentes, Julien A. Nguinkal, Patrick Unger, Bernd Kreikemeyer, Harry W. Palm
Summary: Fish farming in Recirculating Aquaculture Systems (RAS) is a sustainable and efficient practice. This study analyzed the influence of different stocking densities on the water microbiome composition and identified bacterial pathogens associated with common fish farming practices. The results showed that microbiomes differ across systems and sampling points, and fish biomass and water physicochemical properties affect the microbiome.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Environmental Sciences
Eldar Lien, Geir Valsvik, Jan Vidar Nordstrand, Vincent Martinez, Vegard Rogne, Ola Hafsas, Sara Queralt, Behnood Sjastad Fathi, Morten Aga
Summary: Hydrogen sulfide (H2S) is toxic to aquaculture species, causing significant economic losses. Current sensors struggle to detect low concentrations of H2S. This paper introduces a real-time monitoring system for sub mu g/L levels of H2S in RAS, providing reliable data on multiple water quality parameters. The system has implications for risk reduction and mitigation of H2S-induced hazards.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Abanaoub Efraim, Mai Saeed, Mariam Ahmed Elbaz, Mohamed Alaa, Noran Ahmed, Rana Adel, Yara Hazem, Einas Elshatoury, Ola M. Gomaa
Summary: This study aims to remove the toxic compound malachite green (MG) in aquaculture using a microbial fuel cell (MFC) as a sustainable solution. The highest degradation of MG was achieved by an MFC inoculated with the bacteria Shewanella chilikensis strain MG22, which resulted in electricity production and an average decolorization efficiency of almost 88%. The treated water was successfully reused multiple times with the help of sterilization methods.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Kittikoon Sucunthowong, Jay H. Lee, Sorawit Powtongsook, Kasidit Nootong
Summary: By cultivating tilapia in an air-tight tank of a recirculating aquaculture system, CO2 is produced for the growth of Scenedesmus armatus in photobioreactors. It was found that the CO2 concentrations in the tank were higher than in the atmosphere, and using the captured CO2 and nitrate from tilapia farm effluent enhanced algal biomass production. This study demonstrates the potential of repurposing CO2 from aquaculture systems to enhance algal cultivation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Review
Fisheries
Stefanos Moschos, Konstantinos Ar. Kormas, Hera Karayanni
Summary: This review examines the microbiome of recirculating aquaculture systems, focusing on dominant genera and families and their metabolic functions. It also highlights the lack of information on archaeal and microeukaryotic communities and prokaryotic-protist interactions in these systems.
REVIEWS IN AQUACULTURE
(2022)
Article
Environmental Sciences
Bao-shan Shi, Xiang-ju Cheng, Hong-zhan Chen, Jun Xie, Zhi-hong Zhou, Shen-qiong Jiang, Xiao-ming Peng, Yu-da Zhang, Dan-tong Zhu, Zhuo-yin Lu
Summary: The study found that 11 antibiotics were detected in RAS, with 11 detected in water samples and 12 in sediment samples, while no antibiotics were found in fish muscles and feed. TWPPs were effective in removing antibiotics from water. Antibiotic concentrations in RAS were lower than those in nearby open aquaculture ponds, indicating better removal efficiency of TWPPs.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Chemistry, Analytical
Alba Romano, Inmaculada Ortiz, Ane M. Urtiaga
Summary: This study presents a comprehensive kinetic analysis and modelling of electrochemically assisted ammonia removal from marine aquaculture waters. The proposed model combines kinetics of chlorine electrogeneration with experimentally determined mechanism and kinetic parameters of break point chlorination reactions. The model has been validated through laboratory experiments, confirming its validity and robustness in analyzing, predicting, and explaining ammonia removal performance in electrochemical treatment of marine RAS water.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Krzysztof Okarma, Piotr Lech, Darius Andriukaitis, Dangirutis Navikas, Agata Korzelecka-Orkisz, Adam Tanski, Krzysztof Formicki
Summary: This paper investigates the use of optical flow algorithms for estimating water flow in fish breeding tanks. A method based on the features of optical flow algorithms is proposed and verified experimentally, showing high correlation with measurement results obtained using professional water-flow meters.
APPLIED SCIENCES-BASEL
(2022)
Article
Engineering, Chemical
Jonathan A. C. Roques, Federico Micolucci, Suguru Hosokawa, Kristina Sundell, Tomonori Kindaichi
Summary: Recirculating aquaculture systems (RAS) are promising for sustainable aquaculture development, but nitrogenous waste accumulation is a limitation. The anammox process shows potential for treating marine wastewater, with marine anammox bacteria Candidatus Scalindua as a promising candidate. However, anammox activity is affected by unknown compounds in RAS wastewater and/or sub-optimum trace elements content, and TE supplementation can help recover activity. Future studies need to identify the unknown factors and determine specific TE requirements for optimal RAS wastewater treatment by Candidatus Scalindua.
Article
Agricultural Engineering
Jani T. Pulkkinen, Anna-Kaisa Ronkanen, Antti Pasanen, Sepideh Kiani, Tapio Kiuru, Juha Koskela, Petra Lindholm-Lehto, Antti-Jussi Lindroos, Muhammad Muniruzzaman, Lauri Solismaa, Bjorn Klove, Jouni Vielma
Summary: The discharge management of Recirculating Aquaculture Systems is crucial for the development of the aquaculture sector, as RAS do not naturally result in low nutrient emissions. Danish commercial trout farms have successfully adopted wetlands and woodchip bioreactors for effluent treatment. The novel "zero-discharge" RAS system in Denmark has shown promising results in reducing nutrient effluent levels and reusing water effectively.
AQUACULTURAL ENGINEERING
(2021)
Article
Agriculture, Multidisciplinary
Rakesh Ranjan, Kata Sharrer, Scott Tsukuda, Christopher Good
Summary: Artificial intelligence can assist in fish production and management decisions in recirculating aquaculture systems. However, the accuracy of machine learning models in this context is dependent on the quality of input images, which face challenges due to underwater conditions. This study explores the effect of various factors on model accuracy for fish detection under RAS production conditions.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2023)
Article
Environmental Sciences
Alireza Neissi, Gholamreza Rafiee, Shadi Rahimi, Hamid Farahmand, Santosh Pandit, Ivan Mijakovic
Summary: The aim of this study was to enrich high-performance microbial communities for the removal of ammonium and nitrite from aquaculture water. Ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were enriched from different environmental water samples and adapted to different temperatures. The microbial communities containing Nitrosomonas oligotropha and Nitrobacter winogradskyi showed the highest removal activity at all temperatures and do not contain any pathogenic bacteria. Using the enriched microbial communities, a significant improvement in the removal of ammonium and nitrite was achieved compared to commonly used communities in biofilters.
Article
Fisheries
Ruixiang Zhao, Jane E. Symonds, Seumas P. Walker, Konstanze Steiner, Chris G. Carter, John P. Bowman, Barbara F. Nowak
Summary: The feed ration is crucial for fish growth performance and gut health, while temperature significantly affects water microbiota. The faecal microbiota is stable, distinct from water or feed microbiota, and dominated by Photobacterium piscicola in samples with higher water content.
Article
Fisheries
Meritxell Grau Butinyac, Valeria Arenas Montano, Jamie Downes, Neil M. Ruane, Elizabeth Ryder, Thomas Staessen, Fintan Egan, Brett Paull, Eoin Murray
Summary: In this study, automated and portable analyzers were used to analyze nitrite and nitrate in situ in freshwater and saline recirculating aquaculture systems. The analyzers used ion chromatography and UV-LED absorbance detection, and provided real-time concentration data through a cellular IoT module and cloud-based dashboard. The performance of the analyzers was comparable to that of accredited lab-based instrumentation.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Leila Jafari, Kim Joao de Jesus Gregersen, Olav Vadstein, Lars-Flemming Pedersen
Summary: This study investigated the treatment efficiency of foam fractionation (FF) in freshwater and found that it can effectively reduce the concentration of microparticles and turbidity. Addition of hydrogen peroxide (H2O2) had a significant antimicrobial effect, and the combination of H2O2 and FF resulted in a significant reduction in bacterial activity. Furthermore, the efficiency of FF was improved by the addition of salt (NaCl).
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Sanni L. Aalto, Elisavet Syropoulou, Kim Joao de Jesus Gregersen, Marja Tiirola, Per Bovbjerg Pedersen, Lars-Flemming Pedersen
Summary: This study examined the effects of ozone treatment and foam fractionation, alone and combined, on the microbiology of recirculating aquaculture systems (RAS) with rainbow trout. The results showed that foam fractionation had minimal effects on microbial abundance and overall community composition. In contrast, ozone treatment had a significant impact on microbial abundance and composition in both free-living and biofilm communities. Combining the two methods did not provide any additional benefits compared to ozone treatment alone. Additionally, foam fractionation led to changes in the nitrifying microbial community in the biofilm. Overall, the results suggest that these two water treatment methods have different mechanisms and may yield different outcomes under long-term application.
Article
Fisheries
Manuel Gesto, Kim Joao de Jesus Gregersen, Lars -Flemming Pedersen
Summary: The combined use of ozonation and foam fractionation significantly improves water quality in freshwater recirculating aquaculture systems (RAS), but has modest effects on the welfare of rainbow trout juveniles.
Article
Fisheries
Wanhe Qi, Peter Vilhelm Skov, Kim Joao de Jesus Gregersen, Lars -Flemming Pedersen
Summary: Biofilm plays a crucial role in the removal and conversion of nutrients in recirculating aquaculture systems (RAS), but monitoring the bacterial activity in biofilm is difficult. In this study, a new method based on intermittent respirometry was demonstrated to selectively estimate bacterial activity in biofilm, and its feasibility and robustness were confirmed through experiments.
Article
Agricultural Engineering
Sanni L. Aalto, Carlos O. Letelier-Gordo, Lars-Flemming Pedersen, Per Bovbjerg Pedersen
Summary: This study compared the performance of biocarriers made of polyethylene (PE) and polypropylene (PP) in recirculating aquaculture systems. The results showed that PP biocarriers had a shorter maturation time and higher removal rates for total ammonium nitrogen (TAN) and nitrite-nitrogen (NO2--N) compared to PE biocarriers. Pigmentation level had minimal effect on nitrification.
AQUACULTURAL ENGINEERING
(2022)
Article
Fisheries
Francisco Furtado, Mette W. Breiland, David Strand, Gerrit Timmerhaus, Danilo Carletto, Lars-Flemming Pedersen, Fernando Afonso, Carlo C. Lazado
Summary: The study investigated the involvement of oxidative stress in amoebic gill disease in Atlantic salmon and evaluated the response of molecular biomarkers to the chemotherapeutic peracetic acid. Results showed that the disease did not cause systemic oxidative stress, but the expression of oxidative stress biomarkers changed when affected fish were exposed to the chemotherapeutic. The gills and olfactory organ demonstrated distinct patterns of gene expression in AGD-infected-PAA-treated fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Kylian Manon Eggink, Ivar Lund, Per Bovbjerg Pedersen, Benni Winding Hansen, Johanne Dalsgaard
Summary: This study found that different rearing substrates can affect the body composition and growth performance of black soldier fly larvae. There is a clear relationship between the nutritional composition of certain waste substrates and the composition of larvae, particularly for fatty acids. The ash content in the substrate is positively correlated with larval weight, while the lipid, protein, amino acid, and chitin content of larvae seem to be more affected by larval development. These findings suggest that the nutritional value of black soldier fly larvae can be tailored by selecting appropriate rearing substrates for further food and feed applications.
Article
Agriculture, Dairy & Animal Science
Julia Eidsmo, Lone Madsen, Lars-Flemming Pedersen, Alfred Jokumsen, Manuel Gesto
Summary: Enriching the living environment of captive fish can improve their welfare, but studies on the feasibility of implementation in real farming scenarios are limited. This study tested the feasibility of using plastic shelters to improve rainbow trout welfare in an organic fish farm. The shelters had no negative effects on fish performance, health, or mortality, but there were no clear shelter-seeking behavioral responses from the fish when disturbed. It is recommended to test enrichment strategies in real scenarios for a relevant part of the fish's life cycle to fully understand their potential for improving welfare in aquaculture.
Article
Fisheries
Christian Skov, Keno Ferter, Niels Jepsen, Lars -Flemming Pedersen, Casper Gundelund, Marc Simon Weltersbach
Summary: Sea trout is a popular recreational angling target species in the Baltic Sea region and beyond. Information about the postrelease impacts of catch and release (C&R) on survival and growth in saline waters is largely missing. This study investigated the impacts on survival and growth of angled and released sea trout in a flow-through seawater raceway, and found that adverse effects of C&R on coastal sea trout may not pose a significant problem for sea trout stocks.
FISHERIES RESEARCH
(2023)
Article
Environmental Sciences
Johanne Dalsgaard, Kim Schon Ekmann, Mikkel Detz Jensen, Per Bovbjerg Pedersen
Summary: This study aimed to develop and document a diet concept for large rainbow trout in brackish water to reduce the environmental impact of net cage fish farming in terms of phosphorous emissions. By reducing the total dietary P content and matching it to fish requirements, the excretion of dissolved P could be reduced by 87% without compromising fish performance.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Agricultural Engineering
Kim Joao de Jesus Gregersen, Lars -Flemming Pedersen
Summary: This study compared the water quality and fish performance between traditional RAS with nitrifying biofilters and systems using heterotrophic N assimilation. The results showed that RAS with carbon addition had better water quality, but the addition of acetate led to feed spill and reduced fish growth.
AQUACULTURAL ENGINEERING
(2023)
Article
Agricultural Engineering
Bence Daniel Kovacs, Kim Joao de Jesus Gregersen, Florian Ruppel, Arndt von Danwitz, Lars-Flemming Pedersen
Summary: Combining protein skimming with carefully selected O3 doses can improve water quality and control critical factors such as bacterial activity and microparticles in commercial seawater RAS facilities.
AQUACULTURAL ENGINEERING
(2023)
Article
Agricultural Engineering
Xiaoyu Huang, Johanne Dalsgaard, Sanni L. Aalto, Ivar Lund, Per Bovbjerg Pedersen
Summary: Dietary phosphorus levels can affect the concentration of PO4-P and microbial activity in RAS water. Lowering dietary phosphorus levels might be an effective tool for microbial management in RAS.
AQUACULTURAL ENGINEERING
(2023)
Article
Microbiology
Wanhe Qi, Peter Vilhelm Skov, Kim Joao de Jesus Gregersen, Lars-Flemming Pedersen
Summary: This study introduces a new method for rapid assessment of microbial activity in biofilms from aquaculture systems. It involves measuring the release of oxygen from the decomposition of hydrogen peroxide to evaluate microbial enzymatic activity in biofilms. This method can be used to assess the colonization status of biofilters or the effect of disinfectants on biofilm activity.
Article
Fisheries
Sanni L. Aalto, Lone Madsen, Lars-Flemming Pedersen
Summary: Dual-staining flow cytometry allows accurate quantification of live and dead cells, making it a potential tool for studying the mode-of-action of disinfection chemicals and supporting microbial control in aquaculture systems.
